| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,004,243 | A→G | intergenic (‑169/+433) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,004,243 | 0 | A | G | 98.4% | 523.0 / ‑2.5 | 121 | intergenic (‑169/+433) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (43/76); total (44/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 139 total aligned reads displayed.
TGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTT > REL606/1004170‑1004315 | gtCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaggat < 1:6212666/76‑1 (MQ=255) gtCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaggat < 1:1806171/76‑1 (MQ=255) tCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGAtt < 2:1037495/76‑1 (MQ=255) tCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGAtt < 1:4371577/76‑1 (MQ=255) cTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGAttt < 1:5434330/76‑1 (MQ=255) cTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGAttt > 1:3837790/1‑76 (MQ=255) cTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGAttt < 2:5632754/76‑1 (MQ=255) cTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGAttt < 1:2321262/76‑1 (MQ=255) cTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGAttt < 1:304312/76‑1 (MQ=255) tttATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTg > 1:1860740/1‑76 (MQ=255) tttATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTg < 2:1263027/76‑1 (MQ=255) tttATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTg > 2:2812418/1‑76 (MQ=255) tttATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTg > 2:3411473/1‑76 (MQ=255) ttATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTgg > 2:1778285/1‑76 (MQ=255) tATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGt < 2:732830/76‑1 (MQ=255) gtgtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTc > 1:3854599/1‑76 (MQ=255) gtgtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTc < 1:2495802/76‑1 (MQ=255) tgtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCa < 2:4449749/76‑1 (MQ=255) tgtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCa > 2:1742861/1‑76 (MQ=255) gtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaa > 2:6743188/1‑76 (MQ=255) gtCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaa < 2:4785091/76‑1 (MQ=255) cTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaaaa > 1:2563747/1‑76 (MQ=255) cTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaaaa < 1:6799753/76‑1 (MQ=255) cTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaaaa < 1:2111038/76‑1 (MQ=255) cTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaaaa < 1:2565436/76‑1 (MQ=255) cTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCaaaa < 2:2305071/76‑1 (MQ=255) aGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaa < 2:1719156/76‑1 (MQ=255) aGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaa < 2:5230570/76‑1 (MQ=255) aGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaa > 1:1523404/1‑76 (MQ=255) aGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaa < 1:5015113/76‑1 (MQ=255) ggCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaaa < 1:2043561/76‑1 (MQ=255) gCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaaaa > 1:6597309/1‑76 (MQ=255) cATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaaaaa > 1:5984457/1‑76 (MQ=255) cATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGaaaaa > 2:3229161/1‑76 (MQ=255) aTCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAat > 2:1650763/1‑76 (MQ=255) aTCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAat < 1:5666796/76‑1 (MQ=255) aTCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAat > 2:1213758/1‑76 (MQ=255) aTCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAat < 2:6169718/76‑1 (MQ=255) cTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAatat > 1:1321683/1‑76 (MQ=255) tttCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATg > 2:6942806/1‑76 (MQ=255) tttCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATg < 2:2661491/76‑1 (MQ=255) ttCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGt > 2:112711/1‑76 (MQ=255) ttCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGt > 1:5884993/1‑76 (MQ=255) tCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTa < 1:1400425/76‑1 (MQ=255) ccATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTaa < 1:5197024/76‑1 (MQ=255) ccATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTaa < 2:308475/76‑1 (MQ=255) ccATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTaa < 1:5047491/76‑1 (MQ=255) cATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAAc < 1:437910/76‑1 (MQ=255) tAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCaa > 2:249627/1‑76 (MQ=255) aaaaCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCaaa < 2:4392702/76‑1 (MQ=255) aaaaCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCaaa < 2:3120381/76‑1 (MQ=255) aaaaCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCaaa < 1:470071/76‑1 (MQ=255) aaaaCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCaaa > 1:3447218/1‑76 (MQ=255) aaaaCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCaaa < 1:3462902/76‑1 (MQ=255) aaaaCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATATGTAACCaaa > 2:3754396/1‑76 (MQ=255) aaCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAg < 2:1196081/76‑1 (MQ=255) aaCTAACGTTTCACTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAg < 2:4957280/76‑1 (MQ=255) aCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGt < 2:3572811/76‑1 (MQ=255) cTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTa < 2:1776372/76‑1 (MQ=255) tAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTaa > 1:5840391/1‑76 (MQ=255) tAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTaa > 1:2842416/1‑76 (MQ=255) aaCGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTaaa > 1:2617220/1‑76 (MQ=255) aaCGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTaaa > 1:3265154/1‑76 (MQ=255) aaCGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTaaa < 1:5053117/76‑1 (MQ=255) cGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAAt > 2:3385201/1‑76 (MQ=255) tttCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAAttt < 2:6405600/76‑1 (MQ=255) tttCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAAttt < 1:4771851/76‑1 (MQ=255) ttCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTa > 1:318552/1‑76 (MQ=255) tCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTaa < 1:2388907/76‑1 (MQ=255) tCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTaa < 2:5871112/76‑1 (MQ=255) gCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAgg > 1:2190409/1‑76 (MQ=255) tACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGaa < 2:1178730/76‑1 (MQ=255) tACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGaa < 2:5232915/76‑1 (MQ=255) cGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACtt < 1:1719312/76‑1 (MQ=255) cGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACtt < 1:4965253/76‑1 (MQ=255) tGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtg > 1:998379/1‑76 (MQ=255) gAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgt > 1:6482143/1‑76 (MQ=255) gAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgt > 1:6837824/1‑76 (MQ=255) aaaGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgtg < 1:5877528/76‑1 (MQ=255) aGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa > 1:5377105/1‑76 (MQ=255) gTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAac < 2:5010675/76‑1 (MQ=255) tGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca > 1:5682555/1‑76 (MQ=255) tGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca < 1:4652794/76‑1 (MQ=255) gCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAacac < 1:3448341/76‑1 (MQ=255) tACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCg > 2:3013544/1‑76 (MQ=255) tACAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCg < 2:5913411/76‑1 (MQ=255) aCAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGt > 1:1307127/1‑76 (MQ=255) aCAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGt < 1:4258112/76‑1 (MQ=255) cAAAGATAAGGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTc < 1:610900/76‑1 (MQ=255) aagataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCat > 1:4061023/1‑76 (MQ=255) aagataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCat > 1:5497469/1‑76 (MQ=255) agataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCata < 2:3731441/76‑1 (MQ=255) gataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCatat > 2:5557287/1‑76 (MQ=255) gataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCatat < 1:4212068/76‑1 (MQ=255) gataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCatat < 2:6514762/76‑1 (MQ=255) ataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATAtt > 2:4710995/1‑76 (MQ=255) ataaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATAtt > 2:4020451/1‑76 (MQ=255) taaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATAttt > 2:4934838/1‑76 (MQ=255) taaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATAttt > 1:3062477/1‑76 (MQ=255) taaggatTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATAttt > 1:869443/1‑76 (MQ=255) | TGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTT > REL606/1004170‑1004315 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |