| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,928,343 | G→A | A43T (GCA→ACA) | rho → | transcription termination factor Rho |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,928,343 | 0 | G | A | 99.6% | 951.5 / ‑5.4 | 224 | A43T (GCA→ACA) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (110/113); total (110/114) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 236 total aligned reads displayed.
CGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTT > REL606/3928270‑3928415 | cGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGcacaca < 2:2482233/76‑1 (MQ=255)cGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGcacaca < 2:1353931/76‑1 (MQ=255) aaaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACCCaaa > 2:3740872/1‑76 (MQ=255) aaaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACaaa < 2:4436388/76‑1 (MQ=255) aaaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACaaa < 2:5409855/76‑1 (MQ=255) aaaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACaaa < 2:2370997/76‑1 (MQ=255) aaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAag < 1:1121178/76‑1 (MQ=255) aaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAag > 2:6226419/1‑76 (MQ=255) aaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAag < 1:59620/76‑1 (MQ=255) atatGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAagag > 1:3595093/1‑76 (MQ=255) atatGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAagag > 2:1208639/1‑76 (MQ=255) atGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCCCACAAagaggg > 1:2754526/1‑74 (MQ=255) atGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTg < 1:4897469/76‑1 (MQ=255) tGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCCTCCTGAAGCAGCACACCAAGAgggg > 1:3321952/1‑76 (MQ=255) tGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTgg < 2:2654583/76‑1 (MQ=255) ggggCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGc < 1:3878329/76‑1 (MQ=255) gggCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCg < 1:1515731/76‑1 (MQ=255) gggCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCg < 2:4290645/76‑1 (MQ=255) ggCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGa < 1:4772815/76‑1 (MQ=255) gCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGaa < 2:1645461/76‑1 (MQ=255) cTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAg < 1:4579578/76‑1 (MQ=255) tGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGa < 2:1108520/76‑1 (MQ=255) tGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGa > 2:6001729/1‑76 (MQ=255) ggAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGat < 1:3402613/76‑1 (MQ=255) aaaaCCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGatat < 2:5819169/76‑1 (MQ=255) aaaaCCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGatat < 1:5675695/76‑1 (MQ=255) aaCCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCt < 1:138164/76‑1 (MQ=255) ccTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCCTCCTGAAGCAGCACACAAAGAGTGGCGGAGATATCttt > 2:458510/1‑76 (MQ=255) tGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTgg < 2:2757149/76‑1 (MQ=255) ggCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTggg > 2:3116325/1‑75 (MQ=255) ggCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGt < 1:2149537/76‑1 (MQ=255) ggCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGAAATCttttgg > 2:337311/1‑73 (MQ=255) gCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTg < 1:1816457/76‑1 (MQ=255) gCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTg > 2:1928287/1‑76 (MQ=255) cTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGa > 1:4514928/1‑76 (MQ=255) cTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGa > 2:3617458/1‑76 (MQ=255) cTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGa > 1:5323279/1‑76 (MQ=255) cGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATg > 2:1630874/1‑76 (MQ=255) gTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATgg > 1:608742/1‑76 (MQ=255) tATGCGTAAGCAGGACATTATTTTTGTCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGc > 2:6365291/1‑76 (MQ=255) tATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGc > 1:5180188/1‑76 (MQ=255) tATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGc < 2:2933938/76‑1 (MQ=255) tATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGc > 2:2157721/1‑76 (MQ=255) tATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGc < 1:2472848/76‑1 (MQ=255) tATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGc > 2:6895905/1‑76 (MQ=255) aTGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCg > 1:2374294/1‑76 (MQ=255) aTGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCg > 1:4327407/1‑76 (MQ=255) aTGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCg < 1:2775310/76‑1 (MQ=255) aTGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCg > 2:3063351/1‑76 (MQ=255) aTGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCg < 2:2782654/76‑1 (MQ=255) gCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGTGTa < 1:3350881/76‑1 (MQ=255) gCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTa < 2:5304353/76‑1 (MQ=255) cGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGGACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTAc > 1:6391227/1‑76 (MQ=255) tAAGCAGGACATTATTTTTGCCATCCTGAAGGAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTg > 1:5805392/1‑76 (MQ=255) tAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTg > 1:4665827/1‑76 (MQ=255) aaGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTgg > 2:5001064/1‑76 (MQ=255) aaGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTgg > 2:5939052/1‑76 (MQ=255) aaGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTgg < 2:3982926/76‑1 (MQ=255) gCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGgag < 1:3865215/76‑1 (MQ=255) cAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGgaga < 2:1755867/76‑1 (MQ=255) cAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGgaga > 2:4279936/1‑76 (MQ=255) aGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGat > 2:4133138/1‑76 (MQ=255) aGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGat < 1:1682468/76‑1 (MQ=255) aGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGat < 2:2321603/76‑1 (MQ=255) ggACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGata > 2:3001188/1‑76 (MQ=255) aCATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATAtt > 2:4472531/1‑76 (MQ=255) ttattTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCa < 1:6513596/76‑1 (MQ=255) tattTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAg < 2:3263887/76‑1 (MQ=255) attTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAgg < 2:1909393/76‑1 (MQ=255) tttttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAgga < 2:729511/76‑1 (MQ=255) ttttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggat < 2:3925381/76‑1 (MQ=255) ttttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggat < 2:4819127/76‑1 (MQ=255) ttttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggat > 2:240815/1‑76 (MQ=255) tttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatg < 2:2248392/76‑1 (MQ=255) tttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatg < 2:2541029/76‑1 (MQ=255) ttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgg > 1:3089018/1‑76 (MQ=255) ttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgg < 1:1764038/76‑1 (MQ=255) ttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgg > 1:6760716/1‑76 (MQ=255) ttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAAATATTGCAggatgg > 1:1453574/1‑76 (MQ=255) tGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgga > 1:1076688/1‑76 (MQ=255) tGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgga > 2:866086/1‑76 (MQ=255) tGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgga < 1:1955324/76‑1 (MQ=255) tGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatgga > 1:3962204/1‑76 (MQ=255) gCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatggat < 1:4038237/76‑1 (MQ=255) gCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatggat > 1:4429135/1‑76 (MQ=255) ccATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGAtt > 1:3263856/1‑76 (MQ=255) aTCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTg < 2:922675/76‑1 (MQ=255) aTCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTg < 1:1970198/76‑1 (MQ=255) tCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTgg > 1:1982755/1‑76 (MQ=255) ccTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGt < 1:3766308/76‑1 (MQ=255) gAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGgttt > 1:5445837/1‑75 (MQ=255) gAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGGTtc > 2:787175/1‑76 (MQ=255) aaGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTTGttttc > 2:2832972/1‑74 (MQ=255) aaGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGttttc > 2:6195799/1‑74 (MQ=255) agcagcACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGGTTTGGTTtcct > 1:4457224/1‑76 (MQ=255) agcagcACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTtcct < 2:4871708/76‑1 (MQ=255) agcagcACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGGTtcct > 1:1020525/1‑76 (MQ=255) agcagcACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGCAGATATTGCAGGATGGATTTGGTTtcct < 2:3810866/76‑1 (MQ=255) cagcaCACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTtcctcc > 1:5039727/1‑76 (MQ=255) agcaCACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCg > 1:6934215/1‑76 (MQ=255) | CGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTT > REL606/3928270‑3928415 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |