| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,202,309 | A→G | I84T (ATC→ACC) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,202,309 | 0 | A | G | 99.5% | 902.4 / ‑5.1 | 216 | I84T (ATC→ACC) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (110/104); total (110/106) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
Only 100 of 221 total aligned reads displayed.
GATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGG > REL606/4202236‑4202381 | gATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGtt < 2:799788/76‑1 (MQ=255)gATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGtt < 2:2437539/76‑1 (MQ=255)gATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGtt < 2:6132492/76‑1 (MQ=255) aTAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTg > 1:1207430/1‑76 (MQ=255) aTAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTg < 2:735892/76‑1 (MQ=255) aTAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTg < 1:4188209/76‑1 (MQ=255) tAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGc > 1:4446810/1‑76 (MQ=255) tAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGc > 1:3548877/1‑76 (MQ=255) aGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGcc > 1:6943029/1‑76 (MQ=255) aGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGcc < 1:5319279/76‑1 (MQ=255) aGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGcc > 1:1855083/1‑76 (MQ=255) aGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGcc > 2:2901820/1‑76 (MQ=255) ggTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCa < 1:4290310/76‑1 (MQ=255) ggTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCa < 2:517173/76‑1 (MQ=255) ggTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGGTGCCCa > 1:5920084/1‑76 (MQ=255) gTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCaa > 2:6679472/1‑76 (MQ=255) tGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAAt < 2:3975706/76‑1 (MQ=255) tGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAAt < 2:127230/76‑1 (MQ=255) tGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAAt > 1:1649129/1‑76 (MQ=255) tGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAAt < 2:3488729/76‑1 (MQ=255) aaCAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGt < 2:4868964/76‑1 (MQ=255) cAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTcc > 2:1062402/1‑76 (MQ=255) cAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTcc > 1:3438313/1‑76 (MQ=255) aTCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCa > 1:6878785/1‑76 (MQ=255) aTCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCa < 2:2967067/76‑1 (MQ=255) tCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAg > 1:2746312/1‑76 (MQ=255) tCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAg < 1:132544/76‑1 (MQ=255) cGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAgc < 1:500794/76‑2 (MQ=255) cGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGt < 2:1977217/76‑1 (MQ=255) gCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGtt < 1:3236587/76‑1 (MQ=255) gCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGtt < 2:1126992/76‑1 (MQ=255) cTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTc > 1:3793943/1‑76 (MQ=255) cTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTc > 2:6736692/1‑76 (MQ=255) tAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCg < 2:1793295/76‑1 (MQ=255) tAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCg > 1:105461/1‑76 (MQ=255) tAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCg < 2:3196449/76‑1 (MQ=255) aCAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGcc < 1:1124112/76‑1 (MQ=255) cAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCg < 2:91484/76‑1 (MQ=255) cAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCg > 1:3891666/1‑76 (MQ=255) aaaTTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGa < 2:2971460/76‑1 (MQ=255) aTTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGAcc > 1:2554528/1‑76 (MQ=255) aTTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGAcc < 2:2765784/76‑1 (MQ=255) aTTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGAcc > 1:4395262/1‑76 (MQ=255) aTTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGAcc > 1:4650229/1‑76 (MQ=255) ttACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCt < 1:5793430/76‑1 (MQ=255) ttACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCt < 2:5102180/76‑1 (MQ=255) tACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTg < 2:5859659/76‑1 (MQ=255) tACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTg < 2:4683997/76‑1 (MQ=255) tACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTg < 2:4376716/76‑1 (MQ=255) tACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTg < 2:4314665/76‑1 (MQ=255) tACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTg > 2:413737/1‑76 (MQ=255) tACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTg > 2:1546381/1‑76 (MQ=255) ggCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACg > 2:3424352/1‑76 (MQ=255) ggCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACg > 1:2192451/1‑76 (MQ=255) gcctctGGAGAAATCTGCTGCCGACCATAAAGGCGTGTGCGCCGTTTGCCCAATGTCCCAGTTCGCCGACCTGACg < 1:1643384/74‑1 (MQ=255) ctctGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCa < 2:6584349/76‑1 (MQ=255) tctGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCAc > 1:1476853/1‑76 (MQ=255) tctGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCAc > 1:6391919/1‑76 (MQ=255) tctGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCAc > 2:3808618/1‑76 (MQ=255) ctGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACg < 1:1010609/76‑1 (MQ=255) ctGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACg < 1:4103226/76‑1 (MQ=255) ctGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACg < 2:6005586/76‑1 (MQ=255) ctGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACg > 2:4790506/1‑76 (MQ=255) ctGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACg < 1:5820699/76‑1 (MQ=255) ggAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGaa < 1:887265/76‑1 (MQ=255) agaAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAAc > 2:6420876/1‑76 (MQ=255) aaaGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAAccc > 1:4743560/1‑76 (MQ=255) aaaGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCAGACCTGACGCCCTAAAccc > 2:530756/1‑76 (MQ=255) aaGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCct < 2:703319/76‑1 (MQ=255) aaGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACAAAACCct < 1:1906556/76‑1 (MQ=255) aaGCTCCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCct < 2:4393154/76‑1 (MQ=255) aGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCctg < 2:2452366/76‑1 (MQ=255) aGCTGCTGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCctg < 1:778010/76‑1 (MQ=255) gctgctGCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCctgc > 1:2332655/1‑76 (MQ=255) ctgctgCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCctgct > 2:5117696/1‑76 (MQ=255) ctgctgCCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCctgct > 1:6623059/1‑76 (MQ=255) gctgcCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGt > 2:6171635/1‑76 (MQ=255) gctgcCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGt < 2:5060360/76‑1 (MQ=255) ctgcCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGtt > 2:5918862/1‑76 (MQ=255) ctgcCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGtt < 1:3991736/76‑1 (MQ=255) tgcCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTg < 2:2152118/76‑1 (MQ=255) ggcCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTg < 1:3489241/75‑1 (MQ=255) gcCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGc > 2:2552050/1‑76 (MQ=255) gcCGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGc > 2:3277446/1‑76 (MQ=255) ccGACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCa < 2:6289095/76‑1 (MQ=255) gACCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCAtt > 1:5381597/1‑76 (MQ=255) aCCATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATtg < 2:2885491/76‑1 (MQ=255) ccATAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATtgt > 1:5128357/1‑76 (MQ=255) aTAAAGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTgg < 1:5637258/76‑1 (MQ=255) aaaGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGtt > 1:6754011/1‑76 (MQ=255) aaGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTa > 2:1621191/1‑76 (MQ=255) aagCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAg < 2:2282324/74‑1 (MQ=255) aGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAg < 1:2358018/76‑1 (MQ=255) aGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAg < 1:3966954/76‑1 (MQ=255) aGGCATGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAg < 1:4883663/76‑1 (MQ=255) aTGTGCGCGGGTTGCCCAATGTCCCAGTAAGCTGGCCTGGCGCGCGAAACCCTGCTGTTGCATTGTGGTTAGCAgg < 1:338267/76‑1 (MQ=255) aTGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAgg > 2:4595193/1‑76 (MQ=255) aTGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAgg > 1:6254519/1‑76 (MQ=255) aTGTGCGCCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAgg > 2:2133569/1‑76 (MQ=255) gcCGGTTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTgg > 2:1143924/1‑76 (MQ=255) | GATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGATTGCCCAATGTCCCAGTTCGCCGACCTGACGCACGAAACCCTGCTGTTGCATTGTGGTTAGCAGGCGGTGGG > REL606/4202236‑4202381 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |