BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	569.8 / NA	149	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (57/92); total (57/92)

Only 100 of 175 total aligned reads displayed.

CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193707‑2193851

ccGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATccc                                                                       <  2:2764154/76‑1 (MQ=255)

 cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATcccc                                                                      <  2:4340095/76‑1 (MQ=255)

 cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATcccc                                                                      <  1:2345160/76‑1 (MQ=255)

   aTGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAGCCCCCAAAAAAACAAAAACC‑CCAACccccc                                                                     >  1:5720615/1‑75 (MQ=255)

    tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCccacc                                                                   <  1:3465138/76‑1 (MQ=255)

    tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCccacc                                                                   <  1:1727067/76‑1 (MQ=255)

    tGCATCCGGCGTCCAGGCGCGGCTGGGCTTGCTGGTGGGAATCAGCCCCCAAAAAAAGCAAAACG‑CCCACcccccc                                                                    >  2:599546/1‑74 (MQ=255)

    tGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAG‑CCCCCATAAAAACCAAAAG‑CCAAACcccccc                                                                   >  2:1652795/1‑76 (MQ=255)

     gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCccacca                                                                  <  1:6343032/76‑1 (MQ=255)

      cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAG‑CCCCAATAAAACCAAAACG‑CCAAACccccccca                                                                 >  1:4071785/1‑74 (MQ=255)

      cATCCGGCGTCCAGGCGCGGCTGGGCTGGATGGCGGGAATTAT‑ACCCACTAAAAACAAAACC‑ACAAACCCCTCCAg                                                                 >  2:3252931/1‑76 (MQ=255)

       aTCCGGCGTCCAGGCGCGGCTGGGCTGGGTGTCGGGAAACAG‑CCCCCAAAACAGCAAAAAG‑CCAATCCccacccaa                                                                >  2:3546954/1‑73 (MQ=255)

       aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAG‑CCCCCATAAAAGCCAAACC‑CCAAACCccacccaa                                                                >  1:4271491/1‑73 (MQ=255)

       aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAG‑CCCCAATAAAAGCAAAACG‑CCAAACCCCACCAGa                                                                >  1:2079068/1‑76 (MQ=255)

        tCCGGCGTGCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGAt                                                               <  2:2572385/76‑1 (MQ=255)

        tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGGAAACAG‑CCCCCATAACAGCAAAACG‑CCAATCCCCCCCCGAt                                                               >  2:5032554/1‑76 (MQ=255)

         ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATa                                                              <  1:1021583/76‑1 (MQ=255)

         ccGGCGTCCAGGCGCGGCTGGGCTGGCTTGCTGAAATAAC‑CCCCCATTACAACGAAAAC‑CCCATCCCCCCCAGATa                                                              >  1:321296/1‑76 (MQ=255)

          cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATaa                                                             <  2:225840/76‑1 (MQ=255)

          cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATaa                                                             <  1:4911773/76‑1 (MQ=255)

           ggCGTCCAGGCGCGGCTGTTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATaaa                                                            <  2:1928263/76‑1 (MQ=255)

             cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGc                                                          <  1:5480232/76‑1 (MQ=255)

              gTCCAGGCGCGGCTGGTCTGGCTGTCGGGAAAAAT‑CCCCCATAACATCAAAAAG‑CCAATCCCCACCCGATAAAGCa                                                         >  1:1447179/1‑76 (MQ=255)

              gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCa                                                         <  2:908450/76‑1 (MQ=255)

               tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCAt                                                        <  1:5754903/76‑1 (MQ=255)

                ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATg                                                       <  2:6579699/76‑1 (MQ=255)

                ccAGGCGCGGCTGGGCTTGCCGTCGGGAATCAG‑GCCCAAAAACAGAAAAACGCCCACACCCCACCA‑ATAAAACacg                                                       >  2:6153590/1‑74 (MQ=255)

                ccAGGCGCGGCTGGGCTGGCTGTCGGGAATCAG‑CCCCAAAAACAACAAAAAG‑CCAATCCCCACCAGATAAAACATg                                                       >  2:6789207/1‑76 (MQ=255)

                ccAGGCGCGGCTGGGCTGGCTGTCGGAAAACAG‑CCCCAAAAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATg                                                       >  1:5038324/1‑76 (MQ=255)

                   ggCGCGGCTGGACTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACg                                                    <  2:2924517/76‑1 (MQ=255)

                     cgcgGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGcc                                                  <  1:6439965/76‑1 (MQ=255)

                      gcgGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCt                                                 <  1:4674474/76‑1 (MQ=255)

                        ggCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGt                                               >  1:2805130/1‑76 (MQ=255)

                          cTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             >  1:4990671/1‑76 (MQ=255)

                          cTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             <  2:2822176/76‑1 (MQ=255)

                           tGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  2:3768875/1‑76 (MQ=255)

                            ggTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  1:1780687/1‑76 (MQ=255)

                            ggTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           <  1:4008709/76‑1 (MQ=255)

                              tCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         <  2:6877275/76‑1 (MQ=255)

                              tCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         >  1:3787861/1‑76 (MQ=255)

                               cTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  2:4275371/76‑1 (MQ=255)

                                tGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       <  1:1109456/76‑1 (MQ=255)

                                tGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       <  2:3719581/76‑1 (MQ=255)

                                tGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       >  1:1183707/1‑76 (MQ=255)

                                  gCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                     >  2:5181890/1‑76 (MQ=255)

                                   cTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    >  1:5085143/1‑76 (MQ=255)

                                   cTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    >  2:1868112/1‑76 (MQ=255)

                                     gTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  1:3289408/1‑76 (MQ=255)

                                     gTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  2:3440498/1‑76 (MQ=255)

                                     gTCGGAAAGCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:863229/76‑1 (MQ=255)

                                      tCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  1:3391982/76‑1 (MQ=255)

                                       cGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  1:2840338/76‑1 (MQ=255)

                                         gAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  1:3912395/76‑1 (MQ=255)

                                         gAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              >  2:1041656/1‑76 (MQ=255)

                                         gAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  1:751764/76‑1 (MQ=255)

                                         gAAATCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:4907028/76‑1 (MQ=255)

                                          aaaTCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  2:3001192/1‑76 (MQ=255)

                                          aaaTCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             <  1:1916990/76‑1 (MQ=255)

                                          aaaTCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  1:5890669/1‑76 (MQ=255)

                                           aaTCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            >  2:3164507/1‑76 (MQ=255)

                                           aaTCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            >  1:2044882/1‑76 (MQ=255)

                                           aaTCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            <  2:3904436/76‑1 (MQ=255)

                                           aaTCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            <  2:6040170/76‑1 (MQ=255)

                                            aTCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           >  2:1433533/1‑76 (MQ=255)

                                            aTCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  1:164906/76‑1 (MQ=255)

                                            aTCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  1:4209781/76‑1 (MQ=255)

                                             tCAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          >  1:4414863/1‑76 (MQ=255)

                                              cAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  2:4552177/76‑1 (MQ=255)

                                              cAG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  1:6765376/76‑1 (MQ=255)

                                               aG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  2:2973587/76‑1 (MQ=255)

                                               aG‑CCCCAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  2:6112237/76‑1 (MQ=255)

                                                 gcaccAATAACAGCAAAACG‑CCTATGCCCTGCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                       <  2:4223358/73‑1 (MQ=255)

                                                  ccccAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      >  2:1180050/1‑76 (MQ=255)

                                                    ccAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                    >  1:1477077/1‑76 (MQ=255)

                                                    ccAATAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                    >  2:4108400/1‑76 (MQ=255)

                                                      aaTAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  >  1:4681970/1‑76 (MQ=255)

                                                      aaTAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  >  2:4795183/1‑76 (MQ=255)

                                                       aTAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 <  2:533378/76‑1 (MQ=255)

                                                        tAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                <  1:927243/76‑1 (MQ=255)

                                                        tAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                <  2:3984204/76‑1 (MQ=255)

                                                        tAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                >  2:6168999/1‑76 (MQ=255)

                                                        tAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                <  2:4990671/76‑1 (MQ=255)

                                                        tAACAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                >  2:1820736/1‑76 (MQ=255)

                                                          aCAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg              <  2:2426986/76‑1 (MQ=255)

                                                           cAGCAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             <  2:5440985/76‑1 (MQ=255)

                                                              cAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGGGCATCGCGCAcc          <  1:1090499/76‑1 (MQ=255)

                                                              cAAAACG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc          >  2:5308183/1‑76 (MQ=255)

                                                               aaaaCG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  1:6132006/1‑76 (MQ=255)

                                                               aaaaCG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGAGCATCGCGCACCa         >  2:2289883/1‑76 (MQ=255)

                                                                aaaCG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        <  2:549957/76‑1 (MQ=255)

                                                                aaaCG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        >  2:1283139/1‑76 (MQ=255)

                                                                 aaCG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  1:3707760/1‑76 (MQ=255)

                                                                  aCG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      <  2:2037033/76‑1 (MQ=255)

                                                                   cG‑CCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg     >  2:3968629/1‑76 (MQ=255)

                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  1:5336947/1‑76 (MQ=255)

                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  1:6381408/1‑76 (MQ=255)

                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  2:6298114/1‑76 (MQ=255)

                                                                       cAATCCCCACCAGCTCCCGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:1281471/1‑76 (MQ=255)

                                                                       cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACTGATAGCGCATCGCGCACCAGCCGCca  >  2:6075694/1‑76 (MQ=255)

                                                                       cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:2292744/1‑76 (MQ=255)

CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACG‑CCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193707‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`