Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 1,304,917 Δ1 bp coding (1323/1461 nt) cls ← cardiolipin synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,304,9140A.99.4% 786.8 / ‑1.6 158coding (1326/1461 nt)clscardiolipin synthetase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base . (71/85);  total (72/86)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 187 total aligned reads displayed.

TTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGT  >  REL606/1304839‑1304985
                                                                           |                                                                       
ttttAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACCa                                                                         <  1:153834/76‑1 (MQ=255)
 tttAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACCaa                                                                        <  1:1183470/76‑1 (MQ=255)
   tAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACCaaac                                                                      <  1:3196664/76‑2 (MQ=255)
    aGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACCaaacc                                                                     >  1:2624163/1‑74 (MQ=255)
    aGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACCaaacc                                                                     >  1:160418/1‑74 (MQ=255)
    aGCCACAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCCCCCAAAc                                                                     >  1:4553114/1‑76 (MQ=255)
     gCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACCcaacct                                                                    >  1:3335673/1‑73 (MQ=255)
     gCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACCaaacct                                                                    <  2:4936465/76‑4 (MQ=255)
        aTAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTa                                                                >  2:6010013/1‑76 (MQ=255)
         tAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGGAAGGGCTGGACACAAACCttaa                                                                >  2:542430/1‑74 (MQ=255)
         tAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTAt                                                               <  1:1731484/76‑1 (MQ=255)
         tAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTAt                                                               >  2:5838450/1‑76 (MQ=255)
         tAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTAt                                                               <  1:5459130/76‑1 (MQ=255)
         tAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTAt                                                               <  1:1367765/76‑1 (MQ=255)
          aaaCGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTAtc                                                              >  1:1157139/1‑76 (MQ=255)
          aaaCGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTAtc                                                              >  2:185549/1‑76 (MQ=255)
          aaaCGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTAta                                                              <  1:5232330/76‑2 (MQ=255)
           aaCGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTAtcg                                                             <  1:2835256/76‑1 (MQ=255)
            aCGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTAtcgt                                                            >  2:2679267/1‑76 (MQ=255)
             cGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTAtcgtc                                                           >  1:1712916/1‑76 (MQ=255)
              gggCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTAtcgtcg                                                          <  2:1593066/76‑1 (MQ=255)
               ggCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGa                                                         <  2:4442847/76‑1 (MQ=255)
                  aTCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTg                                                      <  2:1012820/76‑1 (MQ=255)
                   tCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGc                                                     >  1:3976388/1‑76 (MQ=255)
                    cGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGcc                                                    >  1:2892386/1‑76 (MQ=255)
                    cGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGcc                                                    >  1:2513804/1‑76 (MQ=255)
                      aGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCag                                                  <  1:4321297/76‑1 (MQ=255)
                      aGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCCGCACC‑AAACCTTTATCGTCGATTGCCag                                                  <  1:3626338/76‑1 (MQ=255)
                       gCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCaga                                                 <  1:6126125/76‑1 (MQ=255)
                        cAGACGTGAACGTGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCagag                                                <  2:2438013/76‑1 (MQ=255)
                        cAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCagag                                                <  1:763468/76‑1 (MQ=255)
                        cAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCagag                                                >  2:5714506/1‑76 (MQ=255)
                         aGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGt                                               <  1:5613990/76‑1 (MQ=255)
                         aGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGt                                               <  1:4818743/76‑1 (MQ=255)
                         aGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGt                                               <  1:4793498/76‑1 (MQ=255)
                          gACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTa                                              <  2:93088/76‑1 (MQ=255)
                          gACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTa                                              <  1:303114/76‑1 (MQ=255)
                            cGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAAt                                            <  1:830286/76‑1 (MQ=255)
                            cGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAAt                                            <  1:224589/76‑1 (MQ=255)
                              tGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAAtct                                          >  2:2529054/1‑76 (MQ=255)
                              tGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAAtct                                          <  1:3572090/76‑1 (MQ=255)
                               gAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAAtctc                                         >  1:4457346/1‑76 (MQ=255)
                               gAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAAtctc                                         >  1:568519/1‑76 (MQ=255)
                               gAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGACTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAAactc                                         >  2:1773356/1‑76 (MQ=255)
                                aaCGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCg                                        <  1:5719847/76‑1 (MQ=255)
                                     gAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAAtt                                   <  2:5325106/76‑1 (MQ=255)
                                     gAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGGAATCTCGAAAtt                                   >  2:3825213/1‑76 (MQ=255)
                                      aaaTATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAAttt                                  <  2:1234824/76‑1 (MQ=255)
                                      aaaTATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAAttt                                  <  2:4992296/76‑1 (MQ=255)
                                      aaaTATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAAttt                                  <  2:5034476/76‑1 (MQ=255)
                                       aaTATAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTa                                 <  2:1717366/76‑1 (MQ=255)
                                        atatAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGGAAttttg                                >  2:367218/1‑74 (MQ=255)
                                        atatAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAAATTAg                                >  2:2975397/1‑76 (MQ=255)
                                        atatAATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGGAATCTCGAAATTTAg                                >  1:412622/1‑76 (MQ=255)
                                         tataATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGc                               <  2:3689854/76‑1 (MQ=255)
                                         tataATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGc                               <  2:2202285/76‑1 (MQ=255)
                                           taATCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCca                             <  2:4899613/76‑1 (MQ=255)
                                            aaTCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCcac                            <  2:2189820/76‑1 (MQ=255)
                                            aaTCGTCCTGAACGGCGGCAAGGGCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCcac                            >  2:5164276/1‑76 (MQ=255)
                                             aTCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCcaca                           >  1:3385704/1‑76 (MQ=255)
                                             aTCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCcaca                           <  2:4500405/76‑1 (MQ=255)
                                              tCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAg                          <  1:122950/76‑1 (MQ=255)
                                              tCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAg                          <  1:205154/76‑1 (MQ=255)
                                              tCGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAg                          >  1:5983222/1‑76 (MQ=255)
                                               tgTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGa                         >  2:4829625/2‑76 (MQ=255)
                                               cGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGa                         >  1:4169818/1‑76 (MQ=255)
                                               cGTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGGAATCTCGAAATTTAGCCACAGa                         >  1:1438699/1‑76 (MQ=255)
                                                gTCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGAc                        >  1:4361667/1‑76 (MQ=255)
                                                 tCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACt                       <  1:5048165/76‑1 (MQ=255)
                                                 tCCTGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACt                       <  2:4129499/76‑1 (MQ=255)
                                                  ccTGAACGGCGGCGAGGGCTGCATCAAAA‑CTTTATCCTCGCTTGCCAGAGTAATCTCGACATTTCGCCACAGGCTc                      >  2:1307157/1‑76 (MQ=255)
                                                    tGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATTGTCGATTTCCAGAGTAATCTCGAAATTTAGCCACAGACTCCg                    >  2:2287239/1‑76 (MQ=255)
                                                    tGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTccc                    >  1:2441581/1‑75 (MQ=255)
                                                    tGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCg                    <  2:2065021/76‑1 (MQ=255)
                                                    tGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCg                    >  1:2546704/1‑76 (MQ=255)
                                                    tGAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCg                    <  2:2849034/76‑1 (MQ=255)
                                                     gAACGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGc                   <  1:664201/76‑1 (MQ=255)
                                                       aCGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTGGCCACAGACTCCGCat                 <  1:2142791/76‑1 (MQ=255)
                                                       aCGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCat                 <  1:4665956/76‑1 (MQ=255)
                                                       aCGGCGGCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCat                 <  1:2278996/76‑1 (MQ=255)
                                                        cggcggCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCata                >  2:5501158/1‑76 (MQ=255)
                                                        cggcggCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCata                <  1:4969019/76‑1 (MQ=255)
                                                        cggcggCAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCata                >  1:958510/1‑76 (MQ=255)
                                                         ggcggcAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCatat               >  2:2024603/1‑76 (MQ=255)
                                                         ggcggcAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCatat               >  2:145735/1‑76 (MQ=255)
                                                           cggcAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCa             <  1:3105581/76‑1 (MQ=255)
                                                            ggcAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCaa            >  2:4182894/1‑76 (MQ=255)
                                                            ggcAAGGTCTGC‑CCCAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCaa            <  1:3275271/76‑1 (MQ=255)
                                                             gcAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAg           <  1:3672258/76‑1 (MQ=255)
                                                             gcAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAg           <  1:6303265/76‑1 (MQ=255)
                                                              cAAGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAgg          >  2:6230722/1‑76 (MQ=255)
                                                               aaGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGt         >  2:1986520/1‑76 (MQ=255)
                                                               aaGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGt         >  2:3428123/1‑76 (MQ=255)
                                                                aGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGtt        >  1:1886017/1‑76 (MQ=255)
                                                                aGGTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGtt        >  1:583790/1‑76 (MQ=255)
                                                                  gTCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGTTaa      <  1:5919756/76‑1 (MQ=255)
                                                                   tCTGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGTTAAc     <  2:5538213/76‑1 (MQ=255)
                                                                     tGCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACgg   >  2:2150796/1‑76 (MQ=255)
                                                                      gCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGt  >  2:4392092/1‑76 (MQ=255)
                                                                      gCACC‑AAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATAACAAGGTTAACggc  >  2:5787508/1‑75 (MQ=255)
                                                                           |                                                                       
TTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGT  >  REL606/1304839‑1304985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑