Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,515,9090AC52.7% 72.2 / 118.9 74V260G (GTG→GGG) ypfIpredicted hydrolase
Reads supporting (aligned to +/- strand):  ref base A (23/12);  new base C (0/39);  total (23/51)
Fisher's exact test for biased strand distribution p-value = 1.01e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-11
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 113 total aligned reads displayed.

GTTAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATT  >  REL606/2515834‑2515984
                                                                            |                                                                                
gTTAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGacca                                                                                  <  2:322023/76‑1 (MQ=255)
gTTAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGacca                                                                                  <  2:5468410/76‑1 (MQ=255)
  tAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGaccacc                                                                                <  2:235557/76‑1 (MQ=255)
    acaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGGGGCTTCTTccaccaccaa                                                                              >  2:2528043/1‑75 (MQ=255)
    acaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccag                                                                              <  2:3189543/76‑1 (MQ=255)
    acaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccag                                                                              <  1:3419872/76‑1 (MQ=255)
    acaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccag                                                                              <  2:4737282/76‑1 (MQ=255)
    acaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccag                                                                              <  2:1658370/76‑1 (MQ=255)
      aacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGGGGCTTTATTCACCAccagcc                                                                            >  1:4637657/1‑76 (MQ=255)
      aacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccagcc                                                                            <  1:6003089/76‑1 (MQ=255)
      aacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccagcc                                                                            <  1:5249852/76‑1 (MQ=255)
      aacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccagcc                                                                            <  2:2364180/76‑1 (MQ=255)
        cGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAccagccag                                                                          <  2:3999040/76‑1 (MQ=255)
         gTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGGGGGTTTCTCCTCCAccagcccag                                                                         >  1:5591961/1‑73 (MQ=255)
         gTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGGGGCTTTCTCGACCAccacccccg                                                                         >  1:848732/1‑73 (MQ=255)
               ggAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTTCTCCAACACCAACCAGGCgggct                                                                   >  1:5937314/1‑73 (MQ=255)
               ggAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTTATCGCCCACCCGCCCggcggcct                                                                   >  1:1053088/1‑74 (MQ=255)
               gaaaaaCGCGATCCCAGTTGATCCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCtt                                                                   <  2:5721867/74‑1 (MQ=255)
               gaaaaaCGCGAGTCCGTTTGAGGCAACAATGGCACAGGTATGGCTGCGGTTTCAGCGCCCCCCAGCCAGTCGGCtt                                                                   <  2:4614470/74‑1 (MQ=255)
                gAAACCGCGATCCCAGTTAATGCAACAATGGCGCAGGTAGGGCTGCGGCTTCACCGCCCCCCAGCCAGTCGGCTTg                                                                  <  2:5336138/76‑1 (MQ=255)
                gAAACCGCGATACCAGTTGATGCAACAAGGGCGCGGTTTTGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTg                                                                  <  2:6193965/76‑1 (MQ=255)
                 aaaaCGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTTATCCACCACCAGCCCGACGGGTTGc                                                                 >  1:4595157/1‑76 (MQ=255)
                 aaaaCGCGATACCAGTGGATGAAACAATGGCGCGGGTAGGCCGGCGGCTTCATCGCCCACCAGCCAGTCGGCTTGc                                                                 <  2:4537489/76‑1 (MQ=255)
                      gcgATCCCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATc                                                           <  1:5104559/76‑1 (MQ=255)
                       ctaTACGAGTTGATCCAACATTGGGCGGGGTTTGCCTCCGGTTCCAGCGCCCCCCAGCCAGTCGGCTTGCTCATCg                                                          <  2:5997864/74‑1 (MQ=255)
                         atcccAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTGCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCt                                                       <  1:3091258/73‑1 (MQ=255)
                         atcccAGTGGATGCACAAGTGGCGCGGTTATGGCTGCGTCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCt                                                       <  2:56034/73‑1 (MQ=255)
                         aTACCAGTTGATGCAACAGTGGCGCAGGTTTGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCt                                                       <  2:2484893/76‑1 (MQ=255)
                          tcccAGTTGATGCAACAATGGCGCAGGTATGGCGGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTg                                                      <  1:4300631/74‑1 (MQ=255)
                          tcccAGTTGATGCAACAATGCCGCAGTTATGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTg                                                      <  1:1279626/74‑1 (MQ=255)
                          tcccAGTTGATGCAACAAGGGCGCAGGTAGGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTg                                                      <  2:4983034/74‑1 (MQ=255)
                            ccAGTTGATGAAACAATGGCGCAGGTAGGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGc                                                   <  2:4975568/76‑1 (MQ=255)
                             cAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCCCCAGCCCATCGGCTTTCTCATCGCTgggg                                                   >  1:5459915/1‑74 (MQ=255)
                              aGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCACCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTa                                                <  2:4352821/76‑1 (MQ=255)
                               gNTGATGCAACAAGGGCGAAGGTAGGGCTGCGTCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTaa                                               <  1:1389744/76‑1 (MQ=255)
                                 tGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCCACCACCAACCCAGCGGCTTTCTCCACGCCGGGTAACa                                            >  1:2249080/1‑76 (MQ=255)
                                 tGATGCAACAAGGGCGCAGTTTTGCCTGCGGCTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACa                                            <  1:4433109/76‑1 (MQ=255)
                                   atccaacaaGGGCGCGGGTATGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAg                                          <  1:232569/73‑1 (MQ=255)
                                   atacaacaaGGGCGAAGGTATGGCTGCGGCTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAg                                          <  2:1392265/73‑1 (MQ=255)
                                   agccaaaaaTGGCGCAGGTTTGGTGGCGGCTTCATCACCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAg                                          <  1:635987/73‑1 (MQ=255)
                                   aTGCAAGAGTGGCCCATTTATGGCTGCGTCTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAg                                          <  1:4016003/76‑1 (MQ=255)
                                     gCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCCCCAGCCAGTCGGCCTGCTCCTCCCTGGGCAACAAAGc                                        >  1:3307104/1‑76 (MQ=255)
                                     gCAACAAGGCCGCGGTTATGGCTGCGGTTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGc                                        <  2:4672552/76‑1 (MQ=255)
                                     gCAAAAAGGGCGCAGGTATGGCTGCGGCTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGc                                        <  2:3242757/76‑1 (MQ=255)
                                     ccaacaaCGCCGCAGTTATGGCTGCGGCTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGc                                        <  1:4004259/75‑1 (MQ=255)
                                      caacaaTGGCGCAGGTATGGCTGGGGCTTCCTCGAACCCCACCCCGTCCGCCTTGTCATCCCCGGGCCAACAAGGc                                        >  2:1485155/1‑76 (MQ=255)
                                       aacaaTGGCGCAGGTATGGCTGCGGCGTTCACCGCCCCCCAACCAGGCGGCTTTCCCCCCCCTGGCTACCAAggaa                                       >  2:4563760/1‑74 (MQ=255)
                                       aacaaTGGCGCAGGTATGGCTGCGGCTTCATCGACCCCCAGCCCGTCGGCTTGCTCATTGCTGGCCTAAAAAGGCa                                       >  1:3117550/1‑76 (MQ=255)
                                       aacaaGGGCGCGGGTTGGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCAt                                      <  1:2615313/76‑1 (MQ=255)
                                        acaaTGGCGCGGGTATGGCTGCGGCTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATc                                     <  2:5194866/76‑1 (MQ=255)
                                          aagggCGGATGTAGGGCGCCGCCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCg                                   <  1:1375117/73‑1 (MQ=255)
                                          aaTGGCGCAGTTATGGCGGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCg                                   <  1:3602895/76‑1 (MQ=255)
                                          aaTGGCGCAGGTATGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCg                                   <  1:2659820/76‑1 (MQ=255)
                                            gggCGGAGGTAGGCCTGCGTCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGgc                                 <  1:4892398/75‑1 (MQ=255)
                                                  gggTGTGTCTGCGGCTTCACCCCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATa                           <  1:3630750/75‑1 (MQ=255)
                                                  gggTGTGGCTGCGGCTTCATCCCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATa                           <  2:2337434/75‑1 (MQ=255)
                                                  gggTATGGCGGCTGCTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATa                           <  1:178993/75‑1 (MQ=255)
                                                   ggTATGGCTGCGGCTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATaa                          <  1:3061867/76‑1 (MQ=255)
                                                   ggTAGGGCTGCGGCTCCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATaa                          <  1:4052797/76‑1 (MQ=255)
                                                   ggTAGGGCGGCGTCTTCATCCCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATaa                          <  2:2517413/76‑1 (MQ=255)
                                                     tgtGGCTGCGGCTTCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAg                        <  2:3272809/74‑1 (MQ=255)
                                                      aTGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCCTCGCTGGGCAAACAGGCCACCGGGGGCATAAAGc                       >  1:1217003/1‑76 (MQ=255)
                                                      aTGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCCGCGCCATaaaac                       >  1:363544/1‑74 (MQ=255)
                                                       tcgCTGCGCCTCCCTCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCg                      <  1:2839628/74‑1 (MQ=255)
                                                       tGGCTGCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCg                      <  2:5699291/76‑1 (MQ=255)
                                                       tGGCTGCGGCTTCATCGCCCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCg                      <  2:4066226/76‑1 (MQ=255)
                                                       tGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTTCTCCTCGCTGGGTAAACAGGGCACCCGCGCaaaaaaaac                      >  2:1245048/1‑73 (MQ=255)
                                                       gggCTGCGGCTTCACCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCg                      <  1:4914290/75‑1 (MQ=255)
                                                       gggCTGCGCCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCg                      <  1:20665/75‑1 (MQ=255)
                                                        ggCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTTCTCATCGCTGGCTAAACAAGGATCCCGCGCCATAAAACGa                     >  2:1759323/1‑76 (MQ=255)
                                                        ggCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGGTCATCGCCGGGCTAAAAAGGATACCGGGCAATCAAGCGa                     >  2:5910123/1‑76 (MQ=255)
                                                         gCTGCGGCTTCAGCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaa                    <  1:1127074/76‑1 (MQ=255)
                                                         gCTGCGCCTTATTCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaa                    <  1:1479935/76‑1 (MQ=255)
                                                          cggCGGCTTCACCCCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaaa                   <  1:1759323/74‑1 (MQ=255)
                                                           tGCGGCTTCATCGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAAc                  <  2:6283885/76‑1 (MQ=255)
                                                            gCGGCTTCATCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACt                 <  2:5541127/76‑1 (MQ=255)
                                                            gCGGCTTCATCGACCACCAGCCAGTCGGCTTGGTCATCGCCTGGTAACAAAGGCATCCGCGCCAAAAAACGaaaac                 >  1:5167390/1‑74 (MQ=255)
                                                             gggCTCCACCGCCCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACtt                <  2:3553336/75‑1 (MQ=255)
                                                             cGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCCATAAAGCGaaaact                >  2:892676/1‑73 (MQ=255)
                                                             cGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAAGCATCCGGCGCAATAAAGCGAAAAtt                >  2:2549474/1‑76 (MQ=255)
                                                              ggCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTtc               >  1:4513001/1‑76 (MQ=255)
                                                               gCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTtct              >  1:4142677/1‑76 (MQ=255)
                                                                cTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCAACCGGCGCAATAAAGCGAAACCtccc             >  1:5961044/1‑74 (MQ=255)
                                                                  ttCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg            >  2:2917246/1‑76 (MQ=255)
                                                                  ttCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg            >  1:5719535/1‑76 (MQ=255)
                                                                  ttCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAAAAAGGCATCCGGCGCGATAAAGCGAAAATTCTCg            >  1:5387174/1‑76 (MQ=255)
                                                                  ttCACCGCCCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg            <  1:5692960/76‑1 (MQ=255)
                                                                    cATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGcc          >  2:5442580/1‑76 (MQ=255)
                                                                    cATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGcc          >  2:3140976/1‑76 (MQ=255)
                                                                      ccGACCCCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCg        <  2:1624095/75‑1 (MQ=255)
                                                                       cGACCGCCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGc       <  2:1748587/76‑1 (MQ=255)
                                                                       cGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGc       >  1:718159/1‑76 (MQ=255)
                                                                       cGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGc       >  1:179412/1‑76 (MQ=255)
                                                                       cGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGc       >  1:1292614/1‑76 (MQ=255)
                                                                        gACCACCAGCCAGTCGGCTTGCTCATCGCTGGGTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCa      >  2:1536664/1‑76 (MQ=255)
                                                                        gACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGGGCAATAAAGCGAAACTTCTCGCCCGCa      >  1:5671588/1‑76 (MQ=255)
                                                                        gACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCa      >  1:1990401/1‑76 (MQ=255)
                                                                           caccaGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAAt   >  2:1147948/1‑76 (MQ=255)
                                                                            accaGCCAGTCGGCTTGTTCATCGCTGGCCAACAAGGCATCCGGCGCCATAAAACGGAACATCTCGCCCGCaaaat  >  2:216870/1‑74 (MQ=255)
                                                                            accaGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCCAAtt  >  1:249756/1‑76 (MQ=255)
                                                                            |                                                                                
GTTAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCACCAGCCAGTCGGCTTGCTCATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATT  >  REL606/2515834‑2515984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: