| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,305,660 | 0 | A | C | 35.6% | 418.1 / 19.6 | 177 | intergenic (‑342/+53) | sspA/rpsI | stringent starvation protein A/30S ribosomal protein S9 |
| Reads supporting (aligned to +/- strand): ref base A (105/9); new base C (0/63); total (105/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.23e-38 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 203 total aligned reads displayed.
TTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGA > REL606/3305585‑3305734 | tttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATaaaaaaa < 1:1018896/76‑1 (MQ=255)tttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATaaaaaaa > 2:5610259/1‑76 (MQ=255)tttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATaaaaaaa > 1:4942364/1‑76 (MQ=255) tACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAAcc > 1:4561605/1‑76 (MQ=255) tACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAAcc > 1:4655058/1‑76 (MQ=255) ccTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACccg > 2:152102/1‑76 (MQ=255) ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACccgc > 1:6160745/1‑76 (MQ=255) ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACccgc < 1:1684560/76‑1 (MQ=255) tgtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACccgcc < 1:2424638/76‑1 (MQ=255) gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACccgccg > 2:5336384/1‑76 (MQ=255) gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACccgccg > 2:3140362/1‑76 (MQ=255) gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACccgccg > 2:1223496/1‑76 (MQ=255) gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACccgccg > 1:5055767/1‑76 (MQ=255) gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACccgccg > 1:1748337/1‑76 (MQ=255) gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACccgccg > 2:842389/1‑76 (MQ=255) gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACccgccg > 1:3121464/1‑76 (MQ=255) tctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGa > 1:1190843/1‑76 (MQ=255) tctgtcAGGTGGCAGAAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGTTTTTATCTAAAAAAAACCCCGCCGa < 2:1742457/76‑1 (MQ=255) ctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGaa > 2:290844/1‑76 (MQ=255) ctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGaa > 2:1827344/1‑76 (MQ=255) ctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGaa > 1:255587/1‑76 (MQ=255) ctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGaa > 1:6062125/1‑76 (MQ=255) gtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGc > 1:5856820/1‑76 (MQ=255) tcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCg < 2:2396007/76‑1 (MQ=255) tcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCg > 1:1520329/1‑76 (MQ=255) cAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCgg > 1:3835209/1‑76 (MQ=255) aGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCggg > 2:3504549/1‑76 (MQ=255) ggTGGAAGCAAAAAGTAACTTTCCAGTTTTTCCGCTGATTCATATTTTAACTAAAAAAAACCCCGCCGAAGCGGGt < 2:4697835/76‑1 (MQ=37) gTGGCAGAAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGGtt < 2:3386043/76‑1 (MQ=255) tGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGttt > 1:3939527/1‑76 (MQ=255) ggCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGtttt > 1:2698742/1‑76 (MQ=255) gcagcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGGttttt < 2:866213/76‑1 (MQ=255) gcagcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGGttttt < 2:1990675/76‑1 (MQ=255) gcagcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGGttttt < 2:2412786/76‑1 (MQ=255) gcagcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGttttt > 2:4115036/1‑76 (MQ=255) gcagaaAAAACAAACTTTCCATTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGGttttt < 2:5037576/76‑1 (MQ=37) agcaAAAAGCAATTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGGTTTTTTc < 2:1312486/76‑1 (MQ=255) agcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTc > 1:1362787/1‑76 (MQ=255) gcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGGTTTTTTCg < 2:1366617/76‑1 (MQ=255) aaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGaaa > 2:3936670/1‑76 (MQ=255) aaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGaaa > 2:2736498/1‑76 (MQ=255) aaaaGAAATTTTCCATTTTTTACGTTGTTTCAGTTTTTAGCTAAAAAAAACCCCGCCGAAGCGGGTTTTTTCGaaa < 1:3683655/76‑1 (MQ=25) gCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTg < 2:1592399/76‑1 (MQ=255) cAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGt > 1:3751098/1‑76 (MQ=255) aCTTTCCAGTTTTTACGCTGATTCAGTTTTTAGCTATAAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGttt < 2:4387175/76‑1 (MQ=255) ttttCCATTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGtttt < 1:6118865/75‑1 (MQ=255) ttttCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGtttt < 2:3297120/75‑1 (MQ=255) cTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGtttt < 2:3545597/76‑1 (MQ=255) cTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGtttt > 1:1878304/1‑76 (MQ=255) tttCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTc > 1:3178154/1‑76 (MQ=255) ttCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCt > 1:5364248/1‑76 (MQ=255) tCCATTTTTTACGCTGATTCAGATTTTACCTATAAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTg < 2:732962/76‑1 (MQ=38) tCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTg < 2:1027552/76‑1 (MQ=255) ccAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGc < 2:6072747/76‑1 (MQ=255) ccAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGc > 2:3561735/1‑76 (MQ=255) aGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCg < 2:417769/76‑1 (MQ=255) gTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCgg > 2:6106495/1‑76 (MQ=255) gTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCgg < 2:3098710/76‑1 (MQ=255) tttACGCTGCTTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGCTTTTTCGAAAATTGTTTTCTGCCGGAGc > 2:1444100/1‑76 (MQ=255) tttACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGc > 1:2141011/1‑76 (MQ=255) tttACGCTGATTCAGATTTTAGCTAAAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGc < 1:4723320/76‑1 (MQ=255) tttACGCTGATTCAGATTTTACCAAAAAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGc < 1:4534757/76‑1 (MQ=38) ttACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCa > 1:65840/1‑76 (MQ=255) tACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAg > 1:2217987/1‑76 (MQ=255) tACGCTGATTCAGATTTTAGCTAAAAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAg < 1:2279752/76‑1 (MQ=255) taaTTAAGTTTTAACCTAAAAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGcc < 2:3886494/74‑1 (MQ=25) tGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGcc > 1:4134109/1‑76 (MQ=255) tGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGcc > 2:2447838/1‑76 (MQ=255) tGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGcc > 2:3978742/1‑76 (MQ=255) tGATTAAGTTTTTAGTTAAAAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGcc < 1:3372081/76‑1 (MQ=37) tCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAAtt > 1:4427588/1‑76 (MQ=255) gATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAAc > 2:4053451/1‑76 (MQ=255) ttAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGttt > 2:554447/1‑76 (MQ=255) tAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTg > 1:2540245/1‑76 (MQ=255) tAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTg > 1:2477941/1‑76 (MQ=255) tAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTg > 1:5194539/1‑76 (MQ=255) tAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTg > 1:201206/1‑76 (MQ=255) tAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTg > 1:2846335/1‑76 (MQ=255) atcTAAAAAAACCCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTgg < 2:5562705/74‑1 (MQ=38) aGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTgg > 2:5666670/1‑76 (MQ=255) aGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTgg > 1:5562069/1‑76 (MQ=255) gCTAAAAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGga < 2:5128786/76‑1 (MQ=255) cTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgag > 1:3978912/1‑76 (MQ=255) cTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgag > 2:226035/1‑76 (MQ=255) cTAAAAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgag < 2:527166/76‑1 (MQ=255) atataAAAAACCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgag < 1:3513644/75‑1 (MQ=255) tataAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgaga > 2:21550/1‑76 (MQ=255) taaaaaaaacCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgaga < 2:2330829/73‑1 (MQ=255) taAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAAc > 2:4565794/1‑76 (MQ=255) taAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAAc > 2:1739751/1‑76 (MQ=255) aaaaaaaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACt > 2:2617571/1‑76 (MQ=255) aaaaaaaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACt > 2:3019383/1‑76 (MQ=255) aaaaacCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTg < 1:4110273/76‑1 (MQ=255) aaaaaaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTg > 1:948171/1‑76 (MQ=255) aaaaaaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTg > 1:4240768/1‑76 (MQ=255) aaaaaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGc > 2:576392/1‑76 (MQ=255) aaaaaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGc > 2:4995589/1‑76 (MQ=255) aaaaaCCCGCCGAAGCGGGTTTTTTCGAAAAGTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGc > 2:4920706/1‑76 (MQ=255) aaaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCgg > 2:2785182/1‑76 (MQ=255) aaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGga > 2:5187363/1‑76 (MQ=255) | TTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGA > REL606/3305585‑3305734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |