| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 430,318 | 0 | T | C | 53.3% | ‑5.9 / 11.5 | 15 | D157G (GAC→GGC) | insL‑2 | putative transposase insL for insertion sequence IS186 |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base C (0/8); total (0/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGATACATTCGGGACGCGAACCGAATCCCCGGTCAGC‑AATGCGTATCTCGTCTGCCGTTTGCGCAAATCGGTCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTGAACTGACAGGTATGAGGATCATATC > REL606/430246‑430387 | cgtaTACATTCGGGACGCGAACCGAATCCCCGGTCAGC‑AATGCGTATCTCGTCTGCCGTTTGCGCAAATCGGTCCa < 1:2110423/73‑1 (MQ=1) atgcGGGACGCGAACCGAACCCCCGGTCAGC‑AATGCGTATCTCGTCTGCCGTTTGCGCAAATCGGTCCAGCCGTTc < 1:2768938/73‑1 (MQ=0) atgcGGGACGCGAACCAAATCCCCGGTCAGC‑AATGCGTATCTCGTCTGCCGTTTGCGCAAATCGGTCCAGCCGTTc < 1:4310304/73‑1 (MQ=0) ttggggACGGGAACCGAATCCCGGGTCAGC‑AAGGCGTATCTCGTCTGCCGTTTGCGCAAATCGGCCCAGCCGTTCa < 1:4739813/73‑1 (MQ=1) ggccgcgAACCAAATCCCCGGTCAGC‑AATGTGTATCTCGCCTGCCGTTTGCGCAAATCGGTCCAGCCGTTCAGCGt < 1:6338442/73‑1 (MQ=1) gcaaaCCGAACCCCCGG‑CAGCAAAGGCTTGTCCCGCCTGCCGTTGGCGCAAATCGGCCCAGCCGTTCAGCGTctct < 2:4815798/73‑1 (MQ=0) gatccGAACCCCCGGTCAGC‑AGTGCGTATCTCGTCTGCCGTTTGCGCAAATCGGTCCAGCCGTTCAGCGTCTctgc < 1:4485819/73‑1 (MQ=1) gacccGAACCCCGGGTCAGA‑AATGCGTTTCCCGTCTGCCGTTTGCGCAAATCGGCCCAGCCGTTCAGCGTCTctgc < 2:4025015/73‑1 (MQ=1) ccgggTCAGC‑AATGCGTCTCTCGCCTGCCGTTTGCGCAAATCGGTCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAg < 1:1208834/73‑1 (MQ=1) ccgggTCAGA‑AATGCGTATCTCGTCTGCCGTTTGCGCAAATCGGCCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAg < 2:3379794/73‑1 (MQ=1) ccgggTAAGA‑AATGGTTACCTCGCCTGCCGTTTGCCCAAATCGGCCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAg < 1:2140571/73‑1 (MQ=1) cacc‑AATCCTTATCTCGTCTGCCGTTTGCGCAAATCGGCCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCaaa < 1:3774979/73‑1 (MQ=1) cattGCGTTCCTCGCCTGCCGTTTGCGCAAATCGGTCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATc < 2:138238/73‑1 (MQ=1) gcttATCTCGTCTGCCGTTTGCGAAAACCGGTCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTg < 2:3777848/73‑1 (MQ=1) gtttctcGTCTGCCGTTTGCGCAAATCGGCCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTGaa < 2:5298404/73‑1 (MQ=0) cgccTGCCGTTTGCACAAATCGGCCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTGAACTGACa < 1:2728198/73‑1 (MQ=1) ctcccGTTGCCGCAAACCGCCCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTGAACTGACAGGt < 1:4000700/73‑1 (MQ=1) gcggTTTGCGCACATGGGCCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTGAACTGACAGGTAt < 2:1510742/73‑1 (MQ=1) gcggTTTGCGAAAATCGGTCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTGAACTGACAGGTAt < 2:2407733/73‑1 (MQ=0) ccttttGCGCAAATCGGTCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTGAACTGACAGGTATg < 2:5079287/73‑1 (MQ=1) ccttttGCGCAAAGGGGCCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTGAACTGACAGGTATg < 1:5958792/73‑1 (MQ=1) ccttttGCGCAAACCGCTCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTGAACTGACAGGTATg < 1:725229/73‑1 (MQ=1) gtgtGCGCAAATCGGTCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTGAACTGACAGGTATGAg < 1:1826192/73‑1 (MQ=1) gcccAAATCGGCCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTGAACTGACAGGTATGAGGATc < 1:2329582/73‑1 (MQ=0) cgaaaaTCGGTCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTGAACTGACAGGTATGAGGATCa < 2:2248456/73‑1 (MQ=1) aagcGGCCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTGAACTGACAGGTATGAGGATCATATc < 1:4778871/73‑1 (MQ=0) | CGGATACATTCGGGACGCGAACCGAATCCCCGGTCAGC‑AATGCGTATCTCGTCTGCCGTTTGCGCAAATCGGTCCAGCCGTTCAGCGTCTCTGCTGTCGGTTAGCTCAAAATCAGTGAACTGACAGGTATGAGGATCATATC > REL606/430246‑430387 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |