| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 452,666 | 0 | A | C | 34.9% | 462.1 / 37.0 | 212 | V37G (GTA→GGA) | ybaJ | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (107/31); new base C (0/74); total (107/105) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-32 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 246 total aligned reads displayed.
TAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTG > REL606/452591‑452741 | tAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTa > 1:3992357/1‑76 (MQ=255)tAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTa < 1:4742096/76‑1 (MQ=255)tAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTa < 1:1341711/76‑1 (MQ=255)tAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTa > 1:2363865/1‑76 (MQ=255) aTTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAcc > 2:4719975/1‑76 (MQ=255) ttAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAccc > 1:1877083/1‑76 (MQ=255) tAAGTGCGAAGGTCGCAATATGCTCAATCGGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCa > 1:1231675/1‑76 (MQ=255) gTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGcc < 1:2860882/76‑1 (MQ=255) tGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCa < 2:1513189/76‑1 (MQ=255) tGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCa < 2:4047468/76‑1 (MQ=255) gCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCAt > 2:1069925/1‑76 (MQ=255) cGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATg < 2:4524322/76‑1 (MQ=255) gAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGa < 2:1896564/76‑1 (MQ=255) gAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGa < 2:6094698/76‑1 (MQ=255) aaGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCGGAGGTTGGGTCGTTTACCCAGCCATGAt < 2:6256362/76‑1 (MQ=255) aaGGTCGCAATATGCTCAATCAGTTCATTCAACGGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGAt < 2:5888117/76‑1 (MQ=255) aGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGAtt < 2:2512350/76‑1 (MQ=255) ggTCGCAATATGCTCAATCAGTTCATTCAACGGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTg < 2:3021645/76‑1 (MQ=255) ggTCGAAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTg < 2:1684937/76‑1 (MQ=255) ggTCGAAATATGCTCAATCAGTTCATTCAACTGGAGGTGGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTg < 2:5575691/76‑1 (MQ=255) gTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGc > 1:1477185/1‑76 (MQ=255) gCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCttt < 2:2456630/76‑1 (MQ=255) gCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCttt < 2:6166061/76‑1 (MQ=255) gCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCttt < 2:5434035/76‑1 (MQ=255) cAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTttc < 2:3130217/76‑1 (MQ=255) aaTATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTttct > 2:5675318/1‑76 (MQ=255) aaTATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTttct > 2:4987216/1‑76 (MQ=255) atatGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTttctt < 2:726637/76‑1 (MQ=255) tatGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTttcttc > 1:5578798/1‑76 (MQ=255) atGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCa > 2:3766616/1‑76 (MQ=255) gCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCTTTTACCCAGCCATGATTGCTTTCTTCAAg < 2:82714/76‑1 (MQ=255) cTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAgg < 1:2279239/76‑1 (MQ=255) cTCAATCAGTTCATTCAACGGGAGTTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAgg < 2:1121286/76‑1 (MQ=255) cAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGtt > 2:5910255/1‑76 (MQ=255) cAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGtt > 1:4213648/1‑76 (MQ=255) cAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGtt > 1:1182686/1‑76 (MQ=255) aaTCAGTTCATTCAACGGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGttt < 2:1994321/76‑1 (MQ=255) tCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGc < 2:647354/76‑1 (MQ=255) cAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCa > 2:3220035/1‑76 (MQ=255) cAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCa > 1:2670888/1‑76 (MQ=255) cAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCa > 2:1777739/1‑76 (MQ=255) cAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCa < 1:6053552/76‑1 (MQ=255) cAGTTCATTCAACTGGAGGTTGATCGCCGAGGTGGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCa < 2:5244022/76‑1 (MQ=255) cAGTTCATTCAACGGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCa < 2:4546320/76‑1 (MQ=255) gTTCATTGAACGGGAGGTTGAGCGCCGGGGTGGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAg < 2:2156626/76‑1 (MQ=255) gTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAg > 1:3588326/1‑76 (MQ=255) ttcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAgg < 2:3378052/76‑1 (MQ=255) tcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGc < 2:2262267/76‑1 (MQ=255) tcattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGc < 1:3573624/76‑1 (MQ=255) cattcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCa > 1:1522635/1‑76 (MQ=255) attcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAg < 2:1610618/76‑1 (MQ=255) tcaACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTc < 1:2129024/76‑1 (MQ=255) tcaACGGGAGGTTGGTCGCCGAGTTTGGTTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTc < 2:2363007/76‑1 (MQ=255) gaaaCTGGAGGTTGACCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTc < 1:3217746/74‑1 (MQ=255) caACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCa > 1:4133718/1‑76 (MQ=255) caACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCa > 1:1714469/1‑76 (MQ=255) caACTGGAGGTTGACCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCa < 2:6020884/76‑1 (MQ=255) aaCGGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCAt < 2:415460/76‑1 (MQ=255) aCTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATg < 1:1019150/76‑1 (MQ=255) aCTGGAGGTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATg < 1:3182280/76‑1 (MQ=255) aCTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATg > 2:5970098/1‑76 (MQ=255) aCTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATg > 1:4413302/1‑76 (MQ=255) tGGAGGTTGATCGCCGAGGTTGGGTCTTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGAt < 1:2440249/76‑1 (MQ=255) ggggTTGATCCCCGAGTTTGGGTCTTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATAc < 2:3850268/74‑1 (MQ=255) gggTTGATCGCCGAGGTTGGGTCTTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACa < 1:111763/75‑1 (MQ=255) gTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAgg < 1:677117/76‑1 (MQ=255) gTTGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAgg < 1:136086/76‑1 (MQ=255) ttGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAggg > 1:5033843/1‑76 (MQ=255) tGATCGCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGt < 1:2604491/76‑1 (MQ=255) tGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGt > 1:2198764/1‑76 (MQ=255) gATCGCCGAGGTTGGGTCTTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGtt < 1:3521022/76‑1 (MQ=255) aTCGCGGAGGTTGGGTCTTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGttt < 2:3462875/76‑1 (MQ=255) aTCGCCGAGTTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGttt < 2:5834550/76‑1 (MQ=255) tccccGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTc < 1:343895/73‑1 (MQ=255) gCCGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcac < 2:5292400/76‑1 (MQ=255) gCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcac > 1:4983598/1‑76 (MQ=255) gCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcac > 2:3661860/1‑76 (MQ=255) gCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcac < 1:2977372/76‑1 (MQ=255) ccGAGGTTGGGTCTTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcaca < 2:173685/76‑1 (MQ=255) cGAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACag < 1:387211/76‑1 (MQ=255) gAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACaga < 2:6282549/76‑1 (MQ=255) gAGGTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACaga < 1:3032715/76‑1 (MQ=255) gAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACaga > 1:4507402/1‑76 (MQ=255) gAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACaga > 1:3407712/1‑76 (MQ=255) gtgTTGGTTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACagag < 1:5311176/74‑1 (MQ=255) gggTTGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACagag < 1:4095574/75‑1 (MQ=255) aGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACagag > 2:1306128/1‑76 (MQ=255) aGGTGGGGTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACagag < 1:5866168/76‑1 (MQ=255) ggttggttCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAgaga < 2:5137502/76‑1 (MQ=255) ggTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAgaga > 1:116759/1‑76 (MQ=255) gTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGaa > 2:1357897/1‑76 (MQ=255) tGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAAc > 1:5468781/1‑76 (MQ=255) ggTCGTTTCCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACtt < 1:5135191/76‑1 (MQ=255) ggTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACtt > 1:5599000/1‑76 (MQ=255) tCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTaa > 2:275809/1‑76 (MQ=255) cGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAg > 2:907374/1‑76 (MQ=255) gTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGc > 2:4115735/1‑76 (MQ=255) gTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGc > 2:3879580/1‑76 (MQ=255) tACCCAGCCATGATTGCTTTCTTCAAGGTTTGCGAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCtgt > 1:6179041/1‑76 (MQ=255) tACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCtgt > 1:2900564/1‑76 (MQ=255) | TAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTG > REL606/452591‑452741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |