BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	475,158	A→G	F50L (TTT→CTT)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	475,158	0	A	G	100.0%	625.6 / NA	156	F50L (TTT→CTT)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (99/57); total (99/57)

Only 100 of 180 total aligned reads displayed.

AACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATT  >  REL606/475086‑475230

aaCCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGcc                                                                       <  2:1862150/76‑1 (MQ=255)

  ccGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCg                                                                     >  1:3133326/1‑76 (MQ=255)

  ccGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCg                                                                     >  1:2296253/1‑76 (MQ=255)

   cGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCgg                                                                    <  1:845983/76‑1 (MQ=255)

    gACGCCACACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAAACAGGCCCGGa                                                                   >  2:4815395/1‑76 (MQ=255)

    gACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGa                                                                   <  1:4800863/76‑1 (MQ=255)

     aCGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAg                                                                  >  2:4051326/1‑76 (MQ=255)

      cGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGt                                                                 <  2:2502544/76‑1 (MQ=255)

       gCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTg                                                                <  2:5623037/76‑1 (MQ=255)

        ccAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGa                                                               >  2:5438640/1‑76 (MQ=255)

         cAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGaa                                                              >  1:2499617/1‑76 (MQ=255)

          aaaCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAAt                                                             >  1:1178365/1‑76 (MQ=255)

           aaCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATg                                                            >  1:682451/1‑76 (MQ=255)

            aCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATgg                                                           <  2:5379062/76‑1 (MQ=255)

            aCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATgg                                                           <  2:4243562/76‑1 (MQ=255)

            aCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATgg                                                           >  2:4530110/1‑76 (MQ=255)

             cATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGGATAGGCAACAAGGCCCGGAGGGAATGGt                                                          >  2:3684331/1‑76 (MQ=255)

               tCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGCGAATGGTcc                                                        >  2:2858422/1‑76 (MQ=255)

                cAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGGGAATGGTCCt                                                       >  1:1574261/1‑76 (MQ=255)

                cAACAGAATGACGCCCAGTCCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCt                                                       <  1:1500044/76‑1 (MQ=255)

                 aaCAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTg                                                      >  2:2474181/1‑76 (MQ=255)

                 aaCAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTg                                                      >  2:1243346/1‑76 (MQ=255)

                 aaCAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTg                                                      <  2:5728136/76‑1 (MQ=255)

                  aCAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGc                                                     <  2:5081194/76‑1 (MQ=255)

                   cAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGcc                                                    <  2:836336/76‑1 (MQ=255)

                   cAGAATGACGCCCAGTTCAGCCAGTTCCGGGACAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGGCCTGcc                                                    >  2:4919957/1‑76 (MQ=255)

                    aGAAGGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCTGCAACAAGGCCCGGAGTGAATGGTCCTGCCa                                                   <  2:4907163/76‑1 (MQ=255)

                     gAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAg                                                  >  2:3210082/1‑76 (MQ=255)

                     gAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAg                                                  >  1:3512354/1‑76 (MQ=255)

                      aaTGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGc                                                 >  2:4597369/1‑76 (MQ=255)

                        tGACGCCCAGTCCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGcac                                               <  1:4795384/76‑1 (MQ=255)

                         gACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGcacc                                              >  2:1330628/1‑75 (MQ=255)

                         gACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGGCCTGCCAGcaca                                              >  2:4165364/1‑76 (MQ=255)

                          ccGCCCGGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCacac                                             <  2:689546/75‑1 (MQ=255)

                          ccGCCCAGTTCAGCCAGTCCCGGGGCAATCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCacac                                             <  2:5871392/75‑1 (MQ=255)

                           cGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACAcc                                            >  2:5564/1‑76 (MQ=255)

                            gCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCCGCACAccc                                           >  2:3563324/1‑76 (MQ=255)

                            gCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACAccc                                           >  1:3910324/1‑76 (MQ=255)

                            gCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACAccc                                           >  2:6073442/1‑76 (MQ=255)

                            gCCAAGTCCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACAccc                                           <  2:1420297/76‑1 (MQ=255)

                             cccAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGGCCTGCCAGCACACCCg                                          >  1:4173327/1‑76 (MQ=255)

                              ccAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGc                                         >  2:2179423/1‑76 (MQ=255)

                              ccAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGGCCTGCCAGCACACCCGc                                         >  1:1887574/1‑76 (MQ=255)

                              caaGTTCAGCCAGTTCCGGGACAACTTTGGTATCGGCAAAAAGGCCCGGAGTGAATGTTCCTGCCAGCACACCCGc                                         <  2:4667000/74‑1 (MQ=255)

                               ctgTTCAGCCGGTTCCGGGGCAAGCTGGGTACCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCt                                        <  2:3863393/74‑1 (MQ=255)

                               cggTTCAGCCGGTTCCGGGGCAGGCTTGGTATCGGCAACAAGCCCGGGAGTGAAGGGTCCTGCCAGCACACCCGCt                                        <  2:2227197/74‑1 (MQ=255)

                               cAGTTCGGCCAGTTCCGGGCCAACCTGGGTATCGCCAACAGGGCCCGGAGTGAATGGCCCTGCCAGCACACCCGCt                                        <  2:2648174/76‑1 (MQ=255)

                               cAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGGCCTGCCAGAACACCCGCt                                        >  2:632745/1‑76 (MQ=255)

                                  ttCAGCCGGTCCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAAc                                     <  1:2484994/76‑1 (MQ=255)

                                  ttCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAAc                                     >  2:4000321/1‑76 (MQ=255)

                                  ttCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAAc                                     <  2:3083595/76‑1 (MQ=255)

                                  tccAGCCAGTTCGGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAAc                                     <  1:1911730/74‑1 (MQ=255)

                                   tCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTTCTGCCAGCACAACCGCTAACa                                    >  1:3885683/1‑76 (MQ=255)

                                   tCAGCCAGTGCCGGGGCAAGCTTGGTATCGGCAACAAGGCCGGGAGTAAATGGTCCTGCCAGCACACCCGCTAACa                                    <  2:2137217/76‑1 (MQ=255)

                                    cAGCCAGTCCGGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAg                                   <  2:4958803/76‑1 (MQ=255)

                                     aGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGa                                  >  2:1798110/1‑76 (MQ=255)

                                     aGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGa                                  >  1:3628393/1‑76 (MQ=255)

                                     aGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTAATGTCAGCAAACCCCCTAACAGa                                  >  1:5311699/1‑76 (MQ=255)

                                      gCCTGTCCCGGGGCAAGTTTGGTATCGGCAACAAGGCCCGGAGTAAAGGGTCCTGCCAGCACACCCGCTAACAGat                                 <  2:5532165/76‑1 (MQ=255)

                                      gCCAGTTCCGGGGCAAGCTTGGTAGCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGat                                 <  2:4491996/76‑1 (MQ=255)

                                        cAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGatat                               >  2:3488727/1‑76 (MQ=255)

                                        cAGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGatat                               <  1:5207589/76‑1 (MQ=255)

                                         aGTTCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGGCCTGCCAGCACACCCGCTAACACATATc                              >  1:95334/1‑76 (MQ=255)

                                         aGTCCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATc                              <  1:377763/76‑1 (MQ=255)

                                          gTTCGGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATcc                             <  2:1522103/76‑1 (MQ=255)

                                            tCCGGGGCAGGCTTGGTACCGGAAACAAGGCCCGGAGTGAATGGTCCTTCCAGCACACCCGCTAACAGATATCcca                           <  1:4558809/76‑1 (MQ=255)

                                            tCCGGGGCAATCTTGGTTTCGGCACCAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCcca                           <  1:2160898/76‑1 (MQ=255)

                                            tCCGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCcca                           <  1:5489412/76‑1 (MQ=255)

                                            gcgggggCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCcca                           <  1:6135742/73‑1 (MQ=255)

                                             ccGGGGCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCccac                          <  1:4897414/76‑1 (MQ=255)

                                                gggCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCag                       <  1:4523942/76‑1 (MQ=255)

                                                 ggaaaGCTGGGTACCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCaga                      <  1:4853362/73‑1 (MQ=255)

                                                 ggCAAGCTTGGTATCGTAAACAAAGCCGGGAGTGAAGGGGCCTGCCAGCACACCCGCTAACAGATATCCCACCaga                      <  1:2388905/76‑1 (MQ=255)

                                                  gCAAGTTTGGTATCGGCAACAAGGCCCGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCagag                     <  1:4417659/76‑1 (MQ=255)

                                                  gCAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCagag                     >  2:3540276/1‑76 (MQ=255)

                                                   cAAGCTTGTTATCGGCAACAAGGCCCGGAGGAAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAgagg                    <  1:3238551/76‑1 (MQ=255)

                                                   cAAGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAgagg                    >  2:1094992/1‑76 (MQ=255)

                                                    aaGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAgagga                   >  2:6166543/1‑76 (MQ=255)

                                                    aaGCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCACCCCAgagga                   >  2:1600871/1‑76 (MQ=255)

                                                     atcTTGTTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAgaggag                  <  1:1188438/74‑1 (MQ=255)

                                                     aGCTGGGTATCGGCAACAAGGCCCGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAgaggag                  <  2:358322/76‑1 (MQ=255)

                                                      gCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGgaga                 >  2:3273488/1‑76 (MQ=255)

                                                      gCTTGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGgaga                 >  2:5499175/1‑76 (MQ=255)

                                                       tttGTTATCGGCAACAGGGCCCGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaa                <  1:438906/75‑1 (MQ=255)

                                                        ttGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaaa               >  2:1147931/1‑76 (MQ=255)

                                                        ttGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaaa               >  1:588096/1‑76 (MQ=255)

                                                         tGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAAt              >  2:5200326/1‑76 (MQ=255)

                                                         tGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAAt              >  1:1653765/1‑76 (MQ=255)

                                                         tGGTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAAt              >  2:3522263/1‑76 (MQ=255)

                                                          ggTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATa             >  2:5355251/1‑76 (MQ=255)

                                                          ggTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATa             >  1:5082257/1‑76 (MQ=255)

                                                          ggTATCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATa             >  1:4252274/1‑76 (MQ=255)

                                                           gTATCGGCAACAAGGCCCGGAGTGAAGGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATAc            <  1:5592508/76‑1 (MQ=255)

                                                            tATCGGCAACAAGGCCCGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACg           <  2:4265022/76‑1 (MQ=255)

                                                             aTCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGt          >  2:5505184/1‑76 (MQ=255)

                                                             aTCGGCAACAAGGCACGGAGTGAATGGTCCTGCCAGCCCACCCGCTACTCCACATCCCAACAGAGGAGAAAAACGt          >  2:2386603/1‑76 (MQ=255)

                                                              tCGGCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTa         <  1:4630541/76‑1 (MQ=255)

                                                                 gCAACAAGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGtt      <  1:3631810/76‑1 (MQ=255)

                                                                  caccaaGGCCCGGATGGAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGttt     <  1:44508/73‑1 (MQ=255)

                                                                  caacaaGGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGttt     >  2:4085941/1‑76 (MQ=255)

AACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATT  >  REL606/475086‑475230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`