| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,163,752 | T→G | I966S (ATC→AGC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,163,752 | 0 | T | G | 98.6% | 598.8 / ‑1.8 | 139 | I966S (ATC→AGC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base G (109/28); total (111/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
Only 100 of 163 total aligned reads displayed.
ACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA > REL606/4163680‑4163824 | aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCt < 2:3232556/76‑1 (MQ=255) cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTc > 2:4169196/1‑76 (MQ=255) cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTc < 2:5441580/76‑1 (MQ=255) aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg > 2:2202442/1‑76 (MQ=255) aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg < 1:1361810/76‑1 (MQ=255) aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCg > 2:1288222/1‑76 (MQ=255) aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGa > 1:2036448/1‑76 (MQ=255) aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGa < 2:4790029/76‑1 (MQ=255) aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGa < 2:2680466/76‑1 (MQ=255) aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGa < 1:1607522/76‑1 (MQ=255) cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAg < 2:5606451/76‑1 (MQ=255) cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAg < 2:5044130/76‑1 (MQ=255) cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGACTCGAAg > 2:4490530/1‑76 (MQ=255) gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCCCGAAGc > 1:3998954/1‑76 (MQ=255) gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGc > 2:3464651/1‑76 (MQ=255) gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGc < 1:1181693/76‑1 (MQ=255) tGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCg < 2:1662759/76‑1 (MQ=255) gcgcTGGGAATCGAAGAAATGCAGCTCAAGCAGGCGGAGAAAGGCCTGTCTGAAGAACTGCAGAGCCTCGAAGCgg < 2:2582946/76‑1 (MQ=255) gcgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCgg < 2:4539985/76‑1 (MQ=255) cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCggg > 2:2488738/1‑76 (MQ=255) cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCggg < 2:4047576/76‑1 (MQ=255) cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCggg < 2:5330202/76‑1 (MQ=255) cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCggg > 1:1781771/1‑76 (MQ=255) gcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGt > 1:2121010/1‑76 (MQ=255) cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTc > 1:3840992/1‑76 (MQ=255) tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCt > 1:4304598/1‑76 (MQ=255) ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTg > 2:5402553/1‑76 (MQ=255) ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTg > 2:3966454/1‑76 (MQ=255) aaaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGtt > 1:2846627/1‑76 (MQ=255) aaaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGtt < 2:1215451/76‑1 (MQ=255) aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑c > 2:5122034/1‑76 (MQ=255) aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑c < 2:887016/76‑1 (MQ=255) aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑c < 2:11250/76‑1 (MQ=255) aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑Ca > 2:1005822/1‑76 (MQ=255) tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAg < 1:4822709/76‑1 (MQ=255) tCGAAGAAATGCAGATCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGTGGGCCTGttaac > 2:6268301/1‑73 (MQ=255) cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGc > 1:2322072/1‑76 (MQ=255) aagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCg < 1:5209511/76‑1 (MQ=255) agaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGt > 2:1456619/1‑76 (MQ=255) aaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATc < 1:1031662/76‑1 (MQ=255) aTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATcc > 1:2504664/1‑76 (MQ=255) tGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCg > 2:3273433/1‑76 (MQ=255) gCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgt > 1:1040728/1‑76 (MQ=255) gCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgt < 1:2977366/76‑1 (MQ=255) cAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtg > 2:4597215/1‑76 (MQ=255) cAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtg > 1:3563811/1‑76 (MQ=255) cAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtg > 2:1147749/1‑76 (MQ=255) gCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgct > 1:3634827/1‑76 (MQ=255) cTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctg > 1:1448733/1‑76 (MQ=255) cTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctg > 2:5220339/1‑76 (MQ=255) tCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgt > 1:4686290/1‑76 (MQ=255) cAAACGGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCttgctg < 2:5225871/74‑1 (MQ=255) cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCttgctg > 1:5225871/1‑74 (MQ=255) cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgtg > 1:4028449/1‑76 (MQ=255) aaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgtgct > 2:942288/1‑76 (MQ=255) cAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTgg > 1:3419762/1‑76 (MQ=255) aGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGt < 1:5578518/76‑1 (MQ=255) aGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGt < 1:468886/76‑1 (MQ=255) ggCGAAGAAAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTa > 1:5836982/1‑76 (MQ=255) gaaaagaaagcCCGGTCTGAAGACCTGCAGACCCTCGAAGGGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAg < 2:2342732/73‑1 (MQ=255) gaagaaAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGcc > 1:5553024/1‑76 (MQ=255) gaagaaAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGcc > 1:4859698/1‑76 (MQ=255) gaagaaAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGcc > 2:3550687/1‑76 (MQ=255) gaagaaAGACCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGCT‑CAGCCGTATCCGTGCTGTGCTGGTAGcc > 1:1315890/1‑76 (MQ=255) aagaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCg > 2:916504/1‑76 (MQ=255) agaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCgg > 1:1838724/1‑76 (MQ=255) gaaagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGt > 2:1572253/1‑76 (MQ=255) acgaCCTGTGAGAAGCCCGGCAGA‑CCCCGGAGGGGGTTTTTTCCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg < 2:2023286/74‑1 (MQ=255) aagaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg > 1:4194119/1‑76 (MQ=255) gaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg > 2:1114075/1‑76 (MQ=255) gaCCTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg > 2:5717173/1‑76 (MQ=255) ccTGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGtt > 2:1215982/1‑76 (MQ=255) tGTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGa > 1:4795468/1‑76 (MQ=255) gttCTGAAGAACGGCAGACCCTGGAAGGGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGa < 1:2884191/74‑1 (MQ=255) gTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa > 2:4755272/1‑76 (MQ=255) gTCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa > 2:5484825/1‑76 (MQ=255) tCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg > 2:5011246/1‑76 (MQ=255) tCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg > 1:526213/1‑76 (MQ=255) tCTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg > 1:4949965/1‑76 (MQ=255) gcagaagaaCTGCAGTGCCCGGAAGGGGTTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg < 1:4085329/73‑1 (MQ=255) cTGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc > 1:3907385/1‑76 (MQ=255) tGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt > 2:6267259/1‑76 (MQ=255) tGAAGAACTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt > 2:3377288/1‑76 (MQ=255) gaagaaCTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg > 2:651603/1‑76 (MQ=255) gaagaaCTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg > 1:6135010/1‑76 (MQ=255) gaagaaCTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg > 2:1766470/1‑76 (MQ=255) agaaCTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag > 2:2904902/1‑76 (MQ=255) aaaaCTGCAGAGCCTCGAAGGGGTTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag < 1:3316125/74‑1 (MQ=255) gaaCTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgaga > 1:2174235/1‑76 (MQ=255) aaccTGCGGAGCCTGGAAGGGGTTTTTTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgaga < 1:1134941/73‑1 (MQ=255) aaccTGCGGAGCCTCGAGGCGGTTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgaga < 1:4451891/73‑1 (MQ=255) aCTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAg > 1:5915409/1‑76 (MQ=255) cTGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGc > 1:5906836/1‑76 (MQ=255) tGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGCAGCt > 2:3769971/1‑76 (MQ=255) tGCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGGGGCGTTGAAGCTGAGAAGCt > 1:497154/1‑76 (MQ=255) ggCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt < 1:6318704/75‑1 (MQ=255) gCAGAGCCTCGAAGGGGGTTTTTC‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc < 1:3702656/76‑1 (MQ=255) gCAGAGCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc > 2:1624522/1‑76 (MQ=255) gCAGACCCTCGAAGGGGGTCTTTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc < 1:488055/76‑1 (MQ=255) agagCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa > 1:2220661/1‑76 (MQ=255) | ACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA > REL606/4163680‑4163824 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |