Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 1,496,226 C→A intergenic (‑134/‑133) ansP ← / → yncG L‑asparagine transporter/predicted enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,496,2260CA98.9% 1180.7 / ‑1.6 281intergenic (‑134/‑133)ansP/yncGL‑asparagine transporter/predicted enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (132/146);  total (132/149)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

ATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGAAAAAATGACAAATCC  >  REL606/1496153‑1496298
                                                                         |                                                                        
aTCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGa                                                                        <  2:1274842/76‑1 (MQ=255)
aTCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGa                                                                        >  2:555740/1‑76 (MQ=255)
aTCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGa                                                                        <  1:1172715/76‑1 (MQ=255)
aTCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGa                                                                        <  2:4451511/76‑1 (MQ=255)
aTCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGa                                                                        <  1:2467072/76‑1 (MQ=255)
aTCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGa                                                                        >  1:2184471/1‑76 (MQ=255)
 tCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGAt                                                                       <  1:1506584/76‑1 (MQ=255)
 tCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGAt                                                                       <  1:4138997/76‑1 (MQ=255)
 tCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGAt                                                                       >  2:802102/1‑76 (MQ=255)
  ccTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATa                                                                      >  2:2781033/1‑76 (MQ=255)
  ccTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATa                                                                      <  1:404840/76‑1 (MQ=255)
   cTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATaa                                                                     <  1:1475367/76‑1 (MQ=255)
   cTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATaa                                                                     >  2:749531/1‑76 (MQ=255)
    tGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATaaa                                                                    <  2:244084/76‑1 (MQ=255)
    tGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATaaa                                                                    <  1:383632/76‑1 (MQ=255)
     ggCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAAc                                                                   >  1:3128742/1‑76 (MQ=255)
       cGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGa                                                                 <  1:1897561/76‑1 (MQ=255)
        gatgatAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGAt                                                                >  2:4649868/1‑76 (MQ=255)
         atgatAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATg                                                               <  2:2421801/76‑1 (MQ=255)
           gatAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCa                                                             >  1:1707676/1‑76 (MQ=255)
           gatAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCa                                                             <  1:1119750/76‑1 (MQ=255)
             tAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCaaa                                                           <  2:5567906/76‑1 (MQ=255)
              aGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCaaaa                                                          <  1:5828882/76‑1 (MQ=255)
                ttATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACa                                                        >  1:5059736/1‑76 (MQ=255)
                 tATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACAt                                                       >  1:2956297/1‑76 (MQ=255)
                  aTCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATc                                                      >  1:3049539/1‑76 (MQ=255)
                    cACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATccc                                                    >  1:2661619/1‑76 (MQ=255)
                      ccATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCt                                                  <  2:4863047/76‑1 (MQ=255)
                      ccATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCt                                                  <  2:3626313/76‑1 (MQ=255)
                      ccATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCt                                                  <  1:2377146/76‑1 (MQ=255)
                      ccATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCt                                                  <  2:89896/76‑1 (MQ=255)
                       cATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCtt                                                 <  1:2348948/76‑1 (MQ=255)
                        attattCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTa                                                <  1:4463768/76‑1 (MQ=255)
                         ttattCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTAc                                               >  1:3720660/1‑76 (MQ=255)
                         ttattCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTAc                                               >  1:6114884/1‑76 (MQ=255)
                           attCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACaa                                             <  2:4278520/76‑1 (MQ=255)
                            ttCTTTTTGGGTATTAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAAt                                            >  2:1630268/1‑76 (MQ=255)
                             tCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATc                                           >  1:5775630/1‑76 (MQ=255)
                             tCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATc                                           >  1:1004462/1‑76 (MQ=255)
                             tCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATc                                           <  1:3768435/76‑1 (MQ=255)
                              ttttGTGGGAATAAAACTATCGGAGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATcc                                          <  2:5742287/75‑1 (MQ=255)
                              cTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATcc                                          >  1:4942743/1‑76 (MQ=255)
                                ttATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTg                                        >  1:4728465/1‑76 (MQ=255)
                                  aTGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGaa                                      <  1:5865846/76‑1 (MQ=255)
                                  aTGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGaa                                      >  1:3507835/1‑76 (MQ=255)
                                   tGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAg                                     >  2:1982352/1‑76 (MQ=255)
                                    gggTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAgg                                    >  2:599920/1‑76 (MQ=255)
                                    gggTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAgg                                    >  1:1246378/1‑76 (MQ=255)
                                     ggTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAggg                                   <  2:2518907/76‑1 (MQ=255)
                                      gTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAgggg                                  <  2:5810947/76‑1 (MQ=255)
                                      gTATAAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAgggg                                  <  1:716638/76‑1 (MQ=255)
                                       tataAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGa                                 <  1:4851299/76‑1 (MQ=255)
                                       tataAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGa                                 <  2:149036/76‑1 (MQ=255)
                                       tataAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGa                                 <  1:1363699/76‑1 (MQ=255)
                                       tataAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGa                                 >  1:3675220/1‑76 (MQ=255)
                                        ataAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGCAGGGGaa                                >  2:4983742/1‑76 (MQ=255)
                                        ataAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGaa                                >  2:3285018/1‑76 (MQ=255)
                                        ataAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGaa                                >  1:6256324/1‑76 (MQ=255)
                                         taAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGaat                               >  2:2455682/1‑76 (MQ=255)
                                         taAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGaat                               >  2:1347074/1‑76 (MQ=255)
                                         taAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGaat                               <  1:1036915/76‑1 (MQ=255)
                                         taAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGaat                               <  1:5257431/76‑1 (MQ=255)
                                         taAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGaat                               <  1:4203452/76‑1 (MQ=255)
                                         taAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGaat                               <  1:6333448/76‑1 (MQ=255)
                                         taAAACTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGaat                               <  1:5290607/76‑1 (MQ=255)
                                         caaagCTGTCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGaat                               <  1:5824030/75‑1 (MQ=255)
                                          aaaaCTATCGGCCCAGCTTCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGaata                              <  1:554463/76‑1 (MQ=255)
                                           aaccTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGaataa                             <  1:282419/73‑1 (MQ=255)
                                            aaCTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGaataat                            >  1:5151052/1‑76 (MQ=255)
                                             aCTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAataata                           <  1:440670/76‑1 (MQ=255)
                                              cTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATAc                          >  1:3352847/1‑76 (MQ=255)
                                              cTATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATAc                          >  2:1695828/1‑76 (MQ=255)
                                               tATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACa                         <  2:5355523/76‑1 (MQ=255)
                                               tATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACa                         >  1:836939/1‑76 (MQ=255)
                                               tATCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACa                         >  1:4225837/1‑76 (MQ=255)
                                                aTCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGACTAATACaa                        >  2:4570425/1‑76 (MQ=255)
                                                aTCGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACaa                        <  1:3412167/76‑1 (MQ=255)
                                                aTCGGCGCAGCATCCTACCCGTAATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACac                        >  2:6128567/1‑75 (MQ=255)
                                                  cGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACt                      >  1:317257/1‑76 (MQ=255)
                                                  cGGCGCAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACt                      >  1:2027618/1‑76 (MQ=255)
                                                    gcgcAGCATCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGa                    <  2:1137559/76‑1 (MQ=255)
                                                      gcagcaTCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACg                  <  2:3422899/76‑1 (MQ=255)
                                                      gcagcaTCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACg                  <  1:2784178/76‑1 (MQ=255)
                                                      gcagcaTCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACg                  >  2:3123207/1‑76 (MQ=255)
                                                      gcagcaTCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACg                  <  1:5460420/76‑1 (MQ=255)
                                                       cagcaTCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGa                 <  2:3839060/76‑1 (MQ=255)
                                                        agcaTCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGaa                >  1:645841/1‑76 (MQ=255)
                                                          caTCCTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGaaaa              >  2:2050580/1‑76 (MQ=255)
                                                             ccTACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGAAAAAAt           >  1:3861727/1‑76 (MQ=255)
                                                               tACCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGAAAAAATGa         <  1:5945909/76‑1 (MQ=255)
                                                                aCCCGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGAAAAAATGAc        <  2:4535303/76‑1 (MQ=255)
                                                                 cccGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGAAAAAATGACa       <  1:4341705/76‑1 (MQ=255)
                                                                 cccGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGAAAAAATGACa       <  1:3522518/76‑1 (MQ=255)
                                                                 cccGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGAAAAAATGACa       <  1:5206920/76‑1 (MQ=255)
                                                                 cccGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGAAAAAATGACa       <  1:2292264/76‑1 (MQ=255)
                                                                  ccGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGAAAAAATGACaa      >  1:255915/1‑76 (MQ=255)
                                                                   cGTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGAAAAAATGACaaa     <  1:4162655/76‑1 (MQ=255)
                                                                    gTTATAGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGAAAAAATGACAAAt    <  2:1063035/76‑1 (MQ=255)
                                                                      tataGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGAAAAAATGACAAATcc  >  1:4502895/1‑76 (MQ=255)
                                                                      tATCGATAAACGATGCAAAACATCCCCATACAATCCTGAAGGGGAATAATACAACTGACGAAAAAATGACAAATcc  <  1:6354583/76‑1 (MQ=255)
                                                                         |                                                                        
ATCCTGGCGATGATAGTTATCACCATTATTCTTTATGGGTATAAAACTATCGGCGCAGCATCCTACCCGTTATCGATAAACGATGCAAAACATCCCCTTACAATCCTGAAGGGGAATAATACAACTGACGAAAAAATGACAAATCC  >  REL606/1496153‑1496298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: