BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	527.7 / NA	139	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (49/90); total (49/90)

Only 100 of 163 total aligned reads displayed.

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851

gcggATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATcc                                                                        <  2:5986415/73‑1 (MQ=255)

gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATcc                                                                        <  2:4971749/76‑1 (MQ=255)

gCCGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGGTGT‑CGGGAATCAGCCCCCATAACAAC‑AAAAAGCCAAAcc                                                                        >  1:1181918/1‑76 (MQ=255)

gCCGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑CGGGAATCAGCCCCAATAACAGC‑AAAAAGCCAATcc                                                                        >  1:2649144/1‑76 (MQ=255)

gCCGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑CGGGAAACAACCCCCAAAACAAC‑AAAACGCCAAccc                                                                        >  1:4937117/1‑76 (MQ=255)

gCCGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGG‑CGGGAAACAGCCCCCATAAAAGC‑AAAAAGCCAAAcc                                                                        >  1:2506412/1‑76 (MQ=255)

gCCGATGCAGCCGGCGTCCAGGCGCGGCTGTTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATcc                                                                        <  2:4179958/76‑1 (MQ=255)

  cGATGCATCCGGCGTCCAGGCGCGGCTGGGGTGGCTGG‑CGGGAAACCGCCCCAATAACATC‑AAAAAGCCAAAcccc                                                                      >  1:4549554/1‑76 (MQ=255)

  cGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑CGGGAAACAGCCCCAAAAACAGC‑AAAAAGCCAAAcccc                                                                      >  2:2931769/1‑76 (MQ=255)

   gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCcca                                                                     <  2:3621910/76‑1 (MQ=255)

   gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGGTGT‑CGGGAATTCGCCCCAATAAACGC‑AAAAAGCCAAAccccc                                                                     >  2:1664772/1‑75 (MQ=255)

       cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAg                                                                 <  2:2062193/76‑1 (MQ=255)

       cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAg                                                                 <  1:1667258/76‑1 (MQ=255)

       cATCCGGCGTCCAGGCGCGGCTGGGGTGGTTGTGCGGAAGTCAGCCCCATCAAC‑GC‑CAAACGCCAAACCccacccg                                                                 >  2:2559403/1‑74 (MQ=255)

         tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGAt                                                               <  1:4969917/76‑1 (MQ=255)

           cGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaa                                                             <  2:2047090/76‑1 (MQ=255)

           cGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaa                                                             <  2:5935258/76‑1 (MQ=255)

           cGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑TGGGAAACAACCCCAAAAAAAACAAAAACACCAACCCCCCCCAgtaa                                                              >  2:5212737/1‑73 (MQ=255)

           cGGCGTCCAGGCGCGGCTGGGCTGGCTGT‑CGGGAATCAGGCCCCAAAACAGC‑AAAACGCCAATCCCCACCCGATaa                                                             >  2:4158787/1‑76 (MQ=255)

            ggCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaaa                                                            <  2:3881625/76‑1 (MQ=255)

            ggCGTCCAGGCGCGGCTGGGCTGGCTGT‑CGGGAATCAGCCCCCATAACAGC‑AAAACGCCAAACCCCACCAGATaaa                                                            >  1:5926869/1‑76 (MQ=255)

              cGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGc                                                          <  1:3817829/76‑1 (MQ=255)

              cGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGc                                                          <  1:3643180/76‑1 (MQ=255)

              cGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGc                                                          <  1:1326908/76‑1 (MQ=255)

              cGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGc                                                          <  1:4702681/76‑1 (MQ=255)

               gTCCAGGCGCGGCTGGGCTGGCTGT‑CGGGAAACAGCCCCAATAACAAC‑AAAAAGCCAATCCCCACCCGATAAAGCa                                                         >  1:5953674/1‑76 (MQ=255)

                tCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCAt                                                        <  1:723790/76‑1 (MQ=255)

                tCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCAt                                                        <  1:1558639/76‑1 (MQ=255)

                 ccAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATg                                                       <  2:58022/76‑1 (MQ=255)

                 ccAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATg                                                       <  1:1580703/76‑1 (MQ=255)

                  cAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGa                                                      <  1:1740460/76‑1 (MQ=255)

                  cAGGCGCGGCTGGGCTGGCTGG‑CGGGAAAAAACCCCCATAAAAAC‑CAAACACCAAACCCCCCCACATCAAACAAGa                                                      >  2:3237316/1‑76 (MQ=255)

                   aGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGAc                                                     <  1:532035/76‑1 (MQ=255)

                        cgGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  2:2548936/76‑1 (MQ=255)

                         ggctgggctgGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                               >  1:2218512/1‑76 (MQ=255)

                         ggCTGGTCTGGCGGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                               <  2:2797880/76‑1 (MQ=255)

                            tGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  2:1758173/76‑1 (MQ=255)

                            tGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  1:233592/1‑76 (MQ=255)

                             ggTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           <  1:1350995/76‑1 (MQ=255)

                             ggTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  2:5116707/1‑76 (MQ=255)

                              gTCTGGCTGT‑CGGCAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATACAGCATGACGCCTGTATTaa                                          >  2:5011497/1‑76 (MQ=255)

                               tCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         >  1:4161184/1‑76 (MQ=255)

                                cTGGGTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCTCCCCAGATAAAGCATGACGCCTGTATTAAAc                                        >  2:100140/1‑76 (MQ=255)

                                cTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  2:4623243/76‑1 (MQ=255)

                                cTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        >  1:3524916/1‑76 (MQ=255)

                                cTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  2:4798232/76‑1 (MQ=255)

                                cTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  1:4201545/76‑1 (MQ=255)

                                  ggCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      >  1:1036201/1‑76 (MQ=255)

                                  ggCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      >  1:3279915/1‑76 (MQ=255)

                                    cTGT‑CGGTAATCAGCCACAAAAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    <  1:6346499/76‑1 (MQ=255)

                                    cTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    <  2:3968164/76‑1 (MQ=255)

                                    cTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    <  1:1277083/76‑1 (MQ=255)

                                    cTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    <  1:6211920/76‑1 (MQ=255)

                                    cTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    >  1:5010743/1‑76 (MQ=255)

                                      gT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  2:2397282/1‑76 (MQ=255)

                                       t‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:1305713/76‑1 (MQ=255)

                                       t‑CGGAAATCAGCCCCAATAACAGC‑AAAACACCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 >  1:621021/1‑76 (MQ=255)

                                         cGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  1:5442782/76‑1 (MQ=255)

                                         cGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  2:4607230/76‑1 (MQ=255)

                                          ggAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:4667220/76‑1 (MQ=255)

                                          ggAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:2282214/76‑1 (MQ=255)

                                           gAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:2618555/76‑1 (MQ=255)

                                           gAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:1957675/76‑1 (MQ=255)

                                            aaaTCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  2:712144/1‑76 (MQ=255)

                                              aTCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  1:3636781/76‑1 (MQ=255)

                                               tCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          <  2:3222200/76‑1 (MQ=255)

                                                cAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         >  2:971744/1‑76 (MQ=255)

                                                cAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         <  2:5035356/76‑1 (MQ=255)

                                                cAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         >  2:3760790/1‑76 (MQ=255)

                                                 aGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  2:3763037/76‑1 (MQ=255)

                                                 aGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  2:5408232/76‑1 (MQ=255)

                                                  gCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                       <  1:2876338/76‑1 (MQ=255)

                                                  gCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                       <  2:2181488/76‑1 (MQ=255)

                                                   ccccAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      <  1:4675217/76‑1 (MQ=255)

                                                   ccccAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      >  1:5522378/1‑76 (MQ=255)

                                                   ccccAATAACAGC‑‑AAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                     >  2:6204130/1‑76 (MQ=255)

                                                      cAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTATCGATGGCGCa                   >  2:5878565/1‑76 (MQ=255)

                                                      cAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                   >  1:577670/1‑76 (MQ=255)

                                                      cAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                   <  2:1433411/76‑1 (MQ=255)

                                                           aCAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg              <  2:2642795/76‑1 (MQ=255)

                                                             aGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa            >  1:2353324/1‑76 (MQ=255)

                                                              gC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           <  2:818881/76‑1 (MQ=255)

                                                               c‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAACCAGGCTTTTTACCGATGGCGCATCGCGCGcc          <  2:2464187/76‑1 (MQ=255)

                                                               c‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCTCatc          >  2:3149674/1‑74 (MQ=255)

                                                               c‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc          <  2:3711307/76‑1 (MQ=255)

                                                                 aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:747336/76‑1 (MQ=255)

                                                                 aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:1275963/76‑1 (MQ=255)

                                                                 aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:5663907/76‑1 (MQ=255)

                                                                  aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        >  2:4059917/1‑76 (MQ=255)

                                                                   aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  2:2984636/1‑76 (MQ=255)

                                                                   aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  2:3566953/1‑76 (MQ=255)

                                                                   aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  1:3511132/1‑76 (MQ=255)

                                                                   aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  2:4509470/1‑76 (MQ=255)

                                                                   aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       <  2:4902023/76‑1 (MQ=255)

                                                                    aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      <  2:5414066/76‑1 (MQ=255)

                                                                    aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      <  2:1659325/76‑1 (MQ=255)

                                                                      gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgc    >  2:6206308/1‑76 (MQ=255)

                                                                       ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGAGCATCGCGCATCAgccccc   >  2:1788919/1‑76 (MQ=255)

                                                                        cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:585797/1‑76 (MQ=255)

                                                                        cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:5083244/1‑76 (MQ=255)

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGT‑CGGAAATCAGCCCCAATAACAGC‑AAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`