| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,489,199 | T→C | Q3R (CAA→CGA) | glpR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,489,199 | 0 | T | C | 98.9% | 764.5 / ‑2.3 | 184 | Q3R (CAA→CGA) | glpR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (86/95); total (88/96) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
Only 100 of 194 total aligned reads displayed.
TCCACCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAA > REL606/3489127‑3489270 | ccaccaGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTgttcgttt > 1:3050046/1‑76 (MQ=255) ccaccaGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTgttcgttt > 2:5530047/1‑76 (MQ=255) accaGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCa < 1:5942264/76‑1 (MQ=255) caGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCAtt > 2:230507/1‑76 (MQ=255) caGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCAtt < 1:5058550/76‑1 (MQ=255) caGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCAtt > 1:807596/1‑76 (MQ=255) aGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCAttt < 2:5831390/76‑1 (MQ=255) gCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTc < 1:4002690/76‑1 (MQ=255) ctcctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCt < 1:4352025/76‑1 (MQ=255) ctcctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCt < 1:4019007/76‑1 (MQ=255) ctcctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCt < 1:6231801/76‑1 (MQ=255) tcctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTa > 2:1850060/1‑76 (MQ=255) cctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTaa > 1:1879003/1‑76 (MQ=255) cctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTaa < 1:4983582/76‑1 (MQ=255) cctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTaa < 1:5696771/76‑1 (MQ=255) ctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTaaa < 2:1174114/76‑1 (MQ=255) ctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTaaa < 1:943250/76‑1 (MQ=255) ctcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTaaa > 1:456472/1‑76 (MQ=255) tcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAAt < 1:3671258/76‑1 (MQ=255) tcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAAt < 2:149681/76‑1 (MQ=255) tcGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTGCATTTCTAAAt < 1:2217619/76‑1 (MQ=255) gTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATccc > 1:5637215/1‑76 (MQ=255) gTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATccc < 1:1410866/76‑1 (MQ=255) aCTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTg < 1:6003324/76‑1 (MQ=255) aCTAACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTg < 1:1717127/76‑1 (MQ=255) cTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTgg > 1:70241/1‑76 (MQ=255) cTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTgg < 2:294862/76‑1 (MQ=255) gACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGaa > 2:4843297/1‑76 (MQ=255) aCATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAat < 1:1091671/76‑1 (MQ=255) aCATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAat < 2:6178103/76‑1 (MQ=255) cATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAatt < 1:1609596/76‑1 (MQ=255) cATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAatt > 2:5804383/1‑76 (MQ=255) cATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAatt < 1:4660158/76‑1 (MQ=255) cATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTCGTGTTCGTTTCATTTCTAAATCCCTGGAatt < 2:4909395/76‑1 (MQ=255) aaCCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAattatt > 2:5256069/1‑76 (MQ=255) ctgctgTTTAACCAGTTCGATAATACCGTTGTGACGTGGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcg < 2:44070/76‑1 (MQ=255) gctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtt < 1:1209337/76‑1 (MQ=255) gctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtt > 1:851754/1‑76 (MQ=255) gctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtt > 1:1352024/1‑76 (MQ=255) gctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtt > 1:2444121/1‑76 (MQ=255) gctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtt > 2:5871600/1‑76 (MQ=255) tgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtttt < 2:4509617/76‑1 (MQ=255) tgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtttt > 1:4681325/1‑76 (MQ=255) tgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgtttt > 1:6128488/1‑76 (MQ=255) gTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgttttc > 2:5980370/1‑76 (MQ=255) gTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTAttttcgttttc > 1:322059/1‑76 (MQ=255) ttAACCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTcgc > 2:4446864/1‑76 (MQ=255) aaCCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCgcgc < 2:4351365/76‑1 (MQ=255) aCCAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCa < 1:1002165/76‑1 (MQ=255) ccAGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCAt > 1:5335304/1‑76 (MQ=255) aGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg < 1:6182479/76‑1 (MQ=255) aGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg < 1:2273644/76‑1 (MQ=255) aGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg < 1:3201096/76‑1 (MQ=255) aGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg < 2:3088022/76‑1 (MQ=255) aGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg < 1:1467129/76‑1 (MQ=255) aGTTCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg < 2:462009/76‑1 (MQ=255) tCGATAATACCGTTGTGACGTTGTGTTCGTTTCATTTTTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGc > 2:3517180/1‑76 (MQ=255) ataataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGaa > 1:5471160/1‑76 (MQ=255) ataataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGaa > 1:4929482/1‑76 (MQ=255) taataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt < 2:1299982/76‑1 (MQ=255) taataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt < 2:1630089/76‑1 (MQ=255) aataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATc < 1:3470224/76‑1 (MQ=255) aataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATc > 2:4235821/1‑76 (MQ=255) aataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATc > 1:2550461/1‑76 (MQ=255) ataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATca < 1:5495984/76‑1 (MQ=255) ataCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATca < 1:4417708/76‑1 (MQ=255) taCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa > 1:1821849/1‑76 (MQ=255) taCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa > 1:3709876/1‑76 (MQ=255) taCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa > 1:2525761/1‑76 (MQ=255) taCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa > 1:3183275/1‑76 (MQ=255) taCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa > 2:2343860/1‑76 (MQ=255) aCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaac > 2:777893/1‑76 (MQ=255) aCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaac > 1:2542413/1‑76 (MQ=255) aCCGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaac < 1:2589176/76‑1 (MQ=255) ccGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaaca < 1:5852543/76‑1 (MQ=255) cGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaacaa > 1:3871701/1‑76 (MQ=255) cGTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaacaa > 2:3957767/1‑76 (MQ=255) gTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa > 1:1992194/1‑76 (MQ=255) gTTGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa < 1:4222779/76‑1 (MQ=255) ttGTGACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaaa < 2:1499047/76‑1 (MQ=255) tgtgACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg < 2:1987754/76‑1 (MQ=255) tgtgACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg > 1:4012323/1‑76 (MQ=255) tgtgACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg > 2:3297361/1‑76 (MQ=255) gtgACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGc < 2:2171627/76‑1 (MQ=255) tgACGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc < 1:3325863/76‑1 (MQ=255) aCGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCAt < 2:2812520/76‑1 (MQ=255) cGTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATc < 1:2151103/76‑1 (MQ=255) gTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATca < 1:4164339/76‑2 (MQ=255) gTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg > 2:5489394/1‑76 (MQ=255) gTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg > 2:1126780/1‑76 (MQ=255) gTTGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg < 1:3571037/76‑1 (MQ=255) ttGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGc > 2:4879042/1‑76 (MQ=255) ttGTGTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGc > 2:5843166/1‑76 (MQ=255) tgtgTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt > 1:5981156/1‑76 (MQ=255) tgtgTTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt > 1:6116334/1‑76 (MQ=255) gtgtTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCGATCGCTa > 1:6250090/1‑76 (MQ=255) gtgtTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTa < 1:2739613/76‑1 (MQ=255) gtgtTCGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTa > 1:4052589/1‑76 (MQ=255) tgttcgttTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaa < 2:3345345/76‑1 (MQ=255) tgttcgttTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaa < 1:2407415/76‑1 (MQ=255) | TCCACCAGCTCCTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAA > REL606/3489127‑3489270 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |