| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,911,972 | (T)7→8 | intergenic (‑173/‑150) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,911,965 | 1 | . | T | 98.5% | 569.1 / 0.7 | 132 | intergenic (‑166/‑157) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base T (30/100); total (30/102) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01 | |||||||||||
Only 100 of 160 total aligned reads displayed.
GAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAA > REL606/3911891‑3912035 | gAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑t < 1:3254467/76‑1 (MQ=255)gAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑t > 1:4842098/1‑76 (MQ=255) aaTGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATCCTGAAATTGAA‑tt > 2:4145052/1‑76 (MQ=255) aaTGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tt > 1:4840386/1‑76 (MQ=255) atgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt < 1:4080152/76‑1 (MQ=255) atgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt < 2:4012745/76‑1 (MQ=255) tgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttt < 1:92934/76‑1 (MQ=255) atgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttttt < 1:356164/76‑1 (MQ=255) atgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttttt > 2:3821267/1‑76 (MQ=255) atgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttttt > 1:7393329/1‑76 (MQ=255) gCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTTAA‑tttttttt < 2:7211224/76‑2 (MQ=255) gCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttttttt > 2:6666120/1‑75 (MQ=255) gCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttttttt < 1:4144335/76‑2 (MQ=255) cTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttttttttc < 1:6758459/76‑3 (MQ=255) cTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttttttttc < 2:5320391/76‑3 (MQ=255) tCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttttttttca < 1:7021603/76‑4 (MQ=255) cAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactc < 1:967113/76‑1 (MQ=255) cAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactc < 1:6087993/76‑1 (MQ=255) cAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactc > 1:4607944/1‑76 (MQ=255) cAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactc < 1:4555087/76‑1 (MQ=255) aaaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactca > 2:2817707/1‑76 (MQ=255) aaaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactca < 2:3089032/76‑1 (MQ=255) aaaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactca > 1:4276260/1‑76 (MQ=255) aaaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactca > 1:1053659/1‑76 (MQ=255) aaaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactca < 1:1515275/76‑1 (MQ=255) aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta < 2:7652978/76‑1 (MQ=255) aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta < 1:3275348/76‑1 (MQ=255) aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta < 1:7784167/76‑1 (MQ=255) aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta > 2:6177194/1‑76 (MQ=255) aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta < 1:1866120/76‑1 (MQ=255) aTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACtatt < 2:667385/76‑1 (MQ=255) tGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACtattt < 1:6589937/76‑1 (MQ=255) gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattt < 2:4337393/76‑1 (MQ=255) gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattt < 2:6002096/76‑1 (MQ=255) gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattt < 2:3755311/76‑1 (MQ=255) gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattt < 2:2754570/76‑1 (MQ=255) gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattt < 2:2110674/76‑1 (MQ=255) gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattt > 2:1371598/1‑76 (MQ=255) cAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatttt < 2:5917706/76‑1 (MQ=255) cAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatttt > 2:1298249/1‑76 (MQ=255) cAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatttt < 1:4801004/76‑1 (MQ=255) aGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatttta < 1:381867/76‑1 (MQ=255) aGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatttta < 1:4863554/76‑1 (MQ=255) gCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattttat < 1:4036763/76‑1 (MQ=255) cGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattttatt < 1:3143781/76‑1 (MQ=255) ggACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattttattt < 2:4182611/76‑1 (MQ=255) ggACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattttattt < 1:2798753/76‑1 (MQ=255) aCAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTAttttt < 2:7486275/76‑1 (MQ=255) aCAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTAttttt < 2:2662125/76‑1 (MQ=255) cAAAGGATAAACTACGAGGAAGGGACCAACATTCATACTGAAATTGAT‑TTTTTTTCACTCACTATTTTATTTTTaa < 2:5558217/76‑1 (MQ=255) aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaa < 2:195498/76‑1 (MQ=255) aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaa < 2:486875/76‑1 (MQ=255) aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaa < 1:74034/76‑1 (MQ=255) aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaa < 1:3800117/76‑1 (MQ=255) aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaa < 1:5104126/76‑1 (MQ=255) aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaac > 1:2702822/1‑76 (MQ=255) tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaaca < 1:7792812/76‑1 (MQ=255) tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaaca > 2:1734846/1‑76 (MQ=255) tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaaca < 2:1463077/76‑1 (MQ=255) tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaaca < 2:6117484/76‑1 (MQ=255) gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaa < 2:4543866/76‑1 (MQ=255) gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaa < 1:1031169/76‑1 (MQ=255) aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAacaaca < 1:5695853/76‑1 (MQ=255) aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAacaaca < 2:1625393/76‑1 (MQ=255) aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAacaaca < 1:5479263/76‑1 (MQ=255) cTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAAcaacaa < 2:1089915/76‑1 (MQ=255) cTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAAcaacaa < 1:2421604/76‑1 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAt > 2:3507236/1‑76 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAt < 2:5902428/76‑1 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAt < 1:6988482/76‑1 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAt > 1:5979166/1‑76 (MQ=255) cGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAttt < 2:2588270/76‑1 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat < 1:759953/76‑1 (MQ=255) ggAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtata < 1:2887356/76‑1 (MQ=255) gAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTatat < 1:2974050/76‑1 (MQ=255) gAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTatat < 2:255528/76‑1 (MQ=255) gAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTatat < 1:2615345/76‑1 (MQ=255) gggAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGa < 1:5274224/76‑1 (MQ=255) aacaacATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAat > 1:3154159/1‑76 (MQ=255) acaacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatt < 2:3947025/76‑1 (MQ=255) acaacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatt < 2:6586652/76‑1 (MQ=255) acaacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatt < 1:1735770/76‑1 (MQ=255) aacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattat > 2:3177123/1‑76 (MQ=255) caTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAAttatta < 1:2209938/76‑1 (MQ=255) caTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAAttatta < 1:6421951/76‑1 (MQ=255) caTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAAttatta < 1:7500968/76‑1 (MQ=255) caTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAAttatta < 1:5535388/76‑1 (MQ=255) aTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaa > 1:7259309/1‑76 (MQ=255) aTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaa > 1:1897944/1‑76 (MQ=255) cATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACg > 1:5966924/1‑76 (MQ=255) cATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACg < 1:7568204/76‑1 (MQ=255) tACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGca > 2:6354731/1‑76 (MQ=255) tACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGca < 1:2549703/76‑1 (MQ=255) gAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat < 1:5191392/76‑1 (MQ=255) gAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat > 2:563838/1‑76 (MQ=255) gAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat < 1:4894215/76‑1 (MQ=255) gAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat < 1:3400042/76‑1 (MQ=255) aTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa > 1:6029785/1‑76 (MQ=255) aTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 1:2567511/76‑1 (MQ=255) ttGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 2:6928012/76‑1 (MQ=255) | GAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAA > REL606/3911891‑3912035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |