| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,995,676 | (C)8→9 | intergenic (+103/+351) | yeeN → / ← asnW | hypothetical protein/tRNA‑Asn |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,995,668 | 1 | . | C | 81.0% | 265.9 / 57.2 | 79 | intergenic (+95/+359) | yeeN/asnW | hypothetical protein/tRNA‑Asn |
| Reads supporting (aligned to +/- strand): ref base . (9/6); new base C (44/20); total (53/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAG > REL606/1995593‑1995740 | gTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATta‑t‑a > 1:2289377/1‑76 (MQ=255) cTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑cc > 1:4058132/1‑76 (MQ=255) cGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑ccccccc < 2:1996997/76‑1 (MQ=255) ggCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑cccccccc < 1:5201634/76‑1 (MQ=255) gCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑ccccccccc > 1:1294641/1‑75 (MQ=255) gCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑ccccccccc < 1:1567858/76‑2 (MQ=255) gCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑ccccccccc < 1:192126/76‑2 (MQ=255) aGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑cccccccccgt < 2:6023991/76‑4 (MQ=255) aGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑cccccccccgt > 2:7716152/1‑73 (MQ=255) ttCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTccc > 2:6727487/1‑76 (MQ=255) cTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCag > 1:3093581/1‑76 (MQ=255) tttAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑ACCCCCCCCCC‑‑G‑TTCCCag > 1:918152/1‑76 (MQ=255) ttAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCagag < 2:2002538/76‑1 (MQ=255) ttAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCagag > 2:713756/1‑76 (MQ=255) ttAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCagag < 2:1851935/76‑1 (MQ=255) tAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑TCC‑‑CCCCCCCC‑‑G‑TTCCCAgaga < 1:7569994/76‑1 (MQ=255) tAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAgaga > 2:3967850/1‑76 (MQ=255) tAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAgaga < 2:3767963/76‑1 (MQ=255) aaTCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGaa < 1:3573587/76‑1 (MQ=255) ctcATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATT‑C‑‑CCCCCCCC‑‑G‑TTCCCAGAGAaata < 1:7716152/76‑1 (MQ=255) ctcATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAaata < 1:6738035/76‑1 (MQ=255) ctcATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAaata > 2:3358487/1‑76 (MQ=255) ctcATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAaataa > 2:5800618/1‑76 (MQ=255) tcATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAaataa > 2:3827651/1‑76 (MQ=255) cATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAaataat > 2:6424007/1‑76 (MQ=255) cATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAaataat > 1:3844062/1‑76 (MQ=255) atatAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAatat < 1:7624003/76‑1 (MQ=255) tataATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAatat > 2:6566002/1‑76 (MQ=255) tataATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAatat < 1:7725703/76‑1 (MQ=255) tataATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAatat < 1:6786030/76‑1 (MQ=255) aaTTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAAtattta > 2:4060416/1‑76 (MQ=255) ttCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTTCCCGAGAAATAAtatttatt > 1:2559928/1‑76 (MQ=255) ttCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATttatta < 1:5274179/76‑1 (MQ=255) tCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATttatta > 1:5546999/1‑76 (MQ=255) tCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATttatta < 2:7107537/76‑1 (MQ=255) tCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATttatta < 1:2963682/76‑1 (MQ=255) tCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTaa > 2:3813555/1‑76 (MQ=255) cTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCCGAGAAATAAtattttttta > 1:4561410/1‑74 (MQ=255) cTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTaa < 1:2279074/76‑1 (MQ=255) ttATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTaaa > 2:38525/1‑76 (MQ=255) ttATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTaaa > 1:2615593/1‑76 (MQ=255) ttATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTaaaa > 2:3686631/1‑76 (MQ=255) tataAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑GCTTCCCA‑AGAAAAAATATTTTTTaaaa > 1:711694/1‑76 (MQ=255) tataAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAAt > 2:1706653/1‑76 (MQ=255) ataAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAAt > 2:3990754/1‑76 (MQ=255) taAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAAtt > 2:3972386/1‑76 (MQ=255) taAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTc < 2:6629477/76‑1 (MQ=255) aaaaaaTATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTc > 2:7867232/1‑76 (MQ=255) aaaaaaTATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTc > 2:7790450/1‑76 (MQ=255) aaaaaaTATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTcc > 2:7790181/1‑76 (MQ=255) aaaaaTATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTcc < 2:3569415/76‑1 (MQ=255) aaaaTATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCa < 2:3273434/76‑1 (MQ=255) aaaTATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAg < 1:648523/76‑1 (MQ=255) aaaTATAATATTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAg > 1:2570369/1‑76 (MQ=255) aatataatatTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGt < 1:1219734/76‑1 (MQ=255) aatataatatTCAACTCGTCATATTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGtt > 2:6740379/1‑76 (MQ=255) atataatatTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGtt < 2:1245907/76‑1 (MQ=255) ataatatTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGttct > 2:4212067/1‑76 (MQ=255) ataatatTCAACTCGTCATATTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGttctt < 1:4932951/76‑1 (MQ=255) atatTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTtcttct > 1:1666611/1‑76 (MQ=255) tatTCAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTcttctt < 1:1266404/76‑1 (MQ=255) cAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTc > 1:2322165/1‑76 (MQ=255) cAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTc > 1:7319398/1‑76 (MQ=255) cAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTc > 1:7425964/1‑76 (MQ=255) cAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTc > 2:7265400/1‑76 (MQ=255) cAACTCGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTc > 2:7695/1‑76 (MQ=255) aCTCGTCATATTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGa > 2:5939388/1‑76 (MQ=255) cTCGTCATATTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAt < 1:7606784/76‑1 (MQ=255) cGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAtt > 1:7747563/1‑76 (MQ=255) cGTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAtt < 1:748427/76‑1 (MQ=255) gTCATATTGATTA‑T‑ACCCCCCCCCC‑‑G‑TTCCCACGGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTTAtt > 1:524761/1‑76 (MQ=255) gTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGGTTa > 1:1121797/1‑76 (MQ=255) gTCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTa > 1:3654875/1‑76 (MQ=255) tCATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAc > 2:3573412/1‑76 (MQ=255) cATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACa < 1:3346668/76‑1 (MQ=255) cATATTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACa > 2:2599140/1‑76 (MQ=255) cATATTGATTA‑T‑A‑‑CCCCCCCCCAG‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAc > 2:2397084/1‑76 (MQ=255) atatTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCCGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTTATTACAg > 1:2308949/1‑76 (MQ=255) atatTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAg > 1:7851665/1‑76 (MQ=255) atatTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAg > 2:1423026/1‑76 (MQ=255) atatTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGa > 1:99255/1‑76 (MQ=255) tatTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGa > 1:67156/1‑76 (MQ=255) tatTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGa > 2:4690155/1‑76 (MQ=255) atTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGaa < 2:6671166/76‑1 (MQ=255) atTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGaa > 2:4499976/1‑76 (MQ=255) atTGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGaa < 2:3185549/76‑1 (MQ=255) ttGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg > 1:5196689/1‑76 (MQ=255) ttGATTA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg > 2:221290/1‑76 (MQ=255) ttA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCaa > 1:3930524/1‑76 (MQ=255) ttA‑T‑AC‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCaa < 1:7615009/76‑1 (MQ=255) ta‑t‑a‑‑CCCCCCCC‑‑G‑TTCCCCGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAg > 1:7014727/1‑76 (MQ=255) | GTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTA‑T‑A‑‑CCCCCCCC‑‑G‑TTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAG > REL606/1995593‑1995740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |