| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,853,067 | 0 | A | C | 38.6% | 255.7 / 136.0 | 179 | T56P (ACC→CCC) | yeaN | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (94/16); new base C (0/69); total (94/85) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-34 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 214 total aligned reads displayed.
ACCGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACGTA > REL606/1852992‑1853142 | aCCGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGacca < 1:2828503/76‑1 (MQ=255)aCCGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGacca > 1:1785440/1‑76 (MQ=255) ccGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGaccac < 2:823051/76‑1 (MQ=255) ccGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGAAAGCGCAAACC‑GG‑CTTATTGaccac > 1:5119142/1‑76 (MQ=255) ggCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCAccc < 1:6727987/76‑1 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCt < 1:1656640/76‑1 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCt < 2:7742277/76‑1 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCt < 1:6210899/76‑1 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCt < 2:1262589/76‑1 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCt < 2:1603102/76‑1 (MQ=255) gcgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCt < 2:1679048/76‑1 (MQ=255) cgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTg < 1:337322/76‑1 (MQ=255) cgcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTg < 1:3899486/76‑1 (MQ=255) cgcAGCACCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTg < 1:5961862/76‑1 (MQ=255) gcAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGc < 1:2356447/76‑1 (MQ=255) cAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGcc < 2:2135039/76‑1 (MQ=255) cAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGcc > 1:5838138/1‑76 (MQ=255) aGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGccc > 1:617158/1‑75 (MQ=255) aGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCa > 1:1340864/1‑76 (MQ=255) aGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCa > 1:1521718/1‑76 (MQ=255) aGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAAAC‑CG‑CCTATTGACCACCCCGccc > 2:5228490/1‑75 (MQ=255) cgcCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCatt > 1:2512092/1‑76 (MQ=255) ccGTTACTGGATACGATTCGTTCCGCTTCCTCGCGGACGACAGCGCAACCC‑GG‑TTTATTGCCCCCCCTGCCattat < 2:134981/76‑1 (MQ=255) ccGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAACGCAAACC‑GG‑CTTATTTACCACCCTGCCattat > 1:6599385/1‑76 (MQ=255) cGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAAAC‑GG‑CTTATTGACCACCCCGCCcttatt > 2:4466200/1‑76 (MQ=255) tACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCAACCTGCCATTATTGGc > 1:122785/1‑76 (MQ=255) cTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCt > 2:6264217/1‑76 (MQ=255) tgaaTAGGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGCCCCCCCTGCCATTATTGGCCtt < 2:662925/73‑1 (MQ=255) tGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCtt > 1:4731179/1‑76 (MQ=255) ggATACGATTCGTTCCTCTTCCTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGCCCCCCCTGCCATTATTGGCCttt < 1:4868378/76‑1 (MQ=255) ggATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGCCCCCCCTGCCATTATTGGCCttt < 1:5022626/76‑1 (MQ=255) ggATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCttt > 2:7095352/1‑76 (MQ=255) ggATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCttt > 1:7247447/1‑76 (MQ=255) ggATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCttt > 1:7162956/1‑76 (MQ=255) ggATAATATTCGTTCCCCTTCCCCGTAGGCGACAGAGCAAACC‑GG‑CTTTTTGACCCCCCTGCCATTATTGGCCttt < 1:3665683/76‑1 (MQ=255) gATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAACCC‑GG‑CTTATTGCCCCCCCTGCCATTATTGGCCTTTg < 2:4148754/76‑1 (MQ=255) atccGATTCTTGCCGTTTCCCCGCTGACGACACCGCAACCC‑GG‑CTTTTTGCCCCCCCTGCCATTATTGGCCTTTgc < 2:647478/73‑1 (MQ=255) aTACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGCCCCCCCTGCCATTATTGGCCTTTgc < 1:1975281/76‑1 (MQ=255) aTACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTgc > 2:5903758/1‑76 (MQ=255) aCGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTgcgc > 2:7431710/1‑76 (MQ=255) cGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCt > 2:3147084/1‑76 (MQ=255) cGATTCGTCCCGCTTCCTCGCTGACGACACCGCAAACC‑GG‑CTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCt < 1:2871137/76‑1 (MQ=255) gATTCGTCCCGTTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTa < 1:4838996/76‑1 (MQ=255) gATCCGTTCCGCTTACTCGCTGACGACAGCCCAAACC‑GG‑CTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTa < 2:3896831/76‑1 (MQ=255) aTTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTaa > 2:287575/1‑76 (MQ=255) aTTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTaa > 2:1878671/1‑76 (MQ=255) ttCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAAt > 2:5478511/1‑76 (MQ=255) cGTTCCGTTTCCTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCCCCCTGCCATTATTGGCCTTTGCGCTAAtct < 2:1132303/76‑1 (MQ=255) gTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAAtctc > 2:1864265/1‑76 (MQ=255) gTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAAtctc > 2:6881651/1‑76 (MQ=255) gTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATAGACCACCCTGCCATTATTGGCCTTTGCGCTAAtctc > 1:544389/1‑76 (MQ=255) ttCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCa > 2:4307660/1‑76 (MQ=255) ttCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCa > 1:3813199/1‑76 (MQ=255) ttCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCa > 2:205609/1‑76 (MQ=255) tcccGCTTCCCCGCTGACGACAGCGCAACCC‑GG‑CTTATTGACCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCa < 2:5507219/74‑1 (MQ=255) tCCTCTTCCTCGCTGACGACAGCGCAACCC‑GG‑CTTATTCCCCCCGCTGCCATTATTGGCCTTTGCGCTAATCTCAc < 2:6084044/76‑1 (MQ=255) tCCTCTTACCCGCTGACGACACAGAAAGCC‑GG‑CTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCAc < 2:7344465/76‑1 (MQ=255) cGCTTCCTCGCGGACGACAGCGCAACCC‑GG‑CTTATTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCt < 2:5009366/76‑1 (MQ=255) ccTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCtt < 2:5330531/75‑1 (MQ=255) acTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCtt > 2:2393575/2‑76 (MQ=255) ttccTCGCTGACGACAGCGCAACCC‑GG‑TTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTg < 1:1497798/73‑1 (MQ=255) ttACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTg > 1:742172/1‑76 (MQ=255) cTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCt > 1:4475021/1‑76 (MQ=255) cGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGc > 2:4945470/1‑76 (MQ=255) cGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGc > 2:681416/1‑76 (MQ=255) cGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGc > 1:1919784/1‑76 (MQ=255) tggcgacAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGcccc > 2:7530628/4‑76 (MQ=255) tGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGcccc > 2:2508820/1‑76 (MQ=255) tgcgacAGCGCAAACC‑GG‑CTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCg < 2:7861510/74‑1 (MQ=255) gacAGCGCAACCC‑GG‑TTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTg < 1:3177689/76‑1 (MQ=255) gacAGCGCAACCC‑GG‑CTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTg < 1:827963/76‑1 (MQ=255) acAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCcggcgg > 1:3682523/1‑76 (MQ=255) cAGCGCAACCC‑GG‑CTTATTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGgc < 2:5784637/76‑1 (MQ=255) aGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGgcg > 2:5625643/1‑76 (MQ=255) cgcAACCC‑GG‑CTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGcgcg < 1:5548994/76‑1 (MQ=255) cccAACCC‑GG‑CTTATGCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGcgcg < 1:684626/74‑1 (MQ=255) gcAAACC‑GG‑CTTATTGACCACCCTGCCATTACTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGa > 2:4917855/1‑76 (MQ=255) caaaaCCGGG‑CTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGcgcg < 2:1319958/74‑1 (MQ=255) cAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGAc > 2:1963062/1‑76 (MQ=255) cAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGAc > 1:4147589/1‑76 (MQ=255) aaccc‑GT‑CTTATTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACg < 2:5285491/73‑1 (MQ=255) aaaCC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACg > 2:3052229/1‑76 (MQ=255) aaCC‑GGTCTTTTTGACCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACg < 2:5600893/76‑1 (MQ=255) cc‑GT‑CTTATTCCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGttt < 2:3364185/76‑1 (MQ=255) c‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGtttt > 2:3176085/1‑76 (MQ=255) gttttATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTg < 2:2793470/73‑1 (MQ=255) cTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGGCGTTTTggg > 2:1744537/1‑75 (MQ=255) cTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGt > 1:7744108/1‑76 (MQ=255) cTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGt > 2:7660727/1‑76 (MQ=255) cTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGt > 1:4767495/1‑76 (MQ=255) tATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTAt > 2:5412162/1‑76 (MQ=255) tATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTAt > 2:3725179/1‑76 (MQ=255) aTTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATg > 1:6061585/1‑76 (MQ=255) tGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGa > 1:5583548/1‑76 (MQ=255) ggACCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGa < 2:5195128/75‑1 (MQ=255) taccaccCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGGCCCGGAGGCGAGACGTTTTGGTATGGaa > 2:3671202/2‑76 (MQ=255) accaccCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAAc > 1:2072027/1‑76 (MQ=255) accaccCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAAc > 1:1088145/1‑76 (MQ=255) accCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACGTa > 2:4612812/1‑76 (MQ=255) accCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACGTa > 2:2631299/1‑76 (MQ=255) | ACCGGCGCAGCGCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACC‑GG‑CTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTGGCGCGACGTTTTGGTATGGAACGTA > REL606/1852992‑1853142 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |