| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,854,401 | 0 | A | C | 48.6% | 111.5 / 54.7 | 109 | K88N (AAA→AAC) | yeaO | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (18/38); new base C (0/53); total (18/91) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.66e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 145 total aligned reads displayed.
CTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGT > REL606/1854326‑1854476 | cTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCaaaaaa < 1:7750424/76‑1 (MQ=255) tCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAg < 1:7202467/76‑1 (MQ=255) cgcgAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGc < 1:4131809/76‑1 (MQ=255) cgcgAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGc < 2:6369234/76‑1 (MQ=255) cgcgAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGc < 1:2142315/76‑1 (MQ=255) cgcgAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGc < 2:737773/76‑1 (MQ=255) gcgAGCAAGATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGcc < 1:1199118/76‑1 (MQ=255) cgAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCg < 2:5609737/76‑1 (MQ=255) gaaaTATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCGGGCGGAC‑ATCGCCAAAAACCAGCCGCTg < 2:1639122/74‑1 (MQ=255) aaTATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGAc < 1:124133/76‑1 (MQ=255) atatCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGCCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGAcc < 1:1156521/76‑1 (MQ=255) tatCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGGGGGCGACGGCCAAAAAAAACCCGC‑GAccc > 2:1304114/1‑76 (MQ=255) atCTTGCAGAACGGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCt < 1:7052305/76‑1 (MQ=255) tCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTg < 1:1050260/76‑1 (MQ=255) tCTGGCAGACCGGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ACCGCCAAAAACCAGCCGCTGACCCTg < 2:2747430/76‑1 (MQ=255) ttGCAGACCGGGCGCAACAGGAGCAAGAGGGAAAGCGGCGGGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGct < 2:6682834/76‑1 (MQ=255) gaaGAACTGGCGAAACACGAAAAAGAAGGAAAGGGGCTGGCGGAC‑ATCGCAAAAAAACAGCCGCTGACCCTGctct < 2:1203480/74‑1 (MQ=255) gaaGAACGGCCGCAACACGAGAAAGAAGGAAGGCGGCTGGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGctct < 2:5085647/74‑1 (MQ=255) gCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGGGGGG‑AACGCCCAAAAACAACCGCCGATCACGctct > 1:1510893/1‑76 (MQ=255) gCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGctct < 1:5309419/76‑1 (MQ=255) gCAGAACGGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGctct < 1:311716/76‑1 (MQ=255) gCAAAACTGGCGCAACGCGAGCAAGAGGGAAAGCGGCTGGCGGAC‑ATCGCAAAAAAACAGCCGCTGACCCTGctct < 1:3490345/76‑1 (MQ=255) cAGACCTGGCGCAACACGAGAAAGAAGGAAAGCGGCGGGCGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTa < 2:285834/76‑1 (MQ=255) aaaaCGGGCGCAACACGAGAAAGAAGAAAAGCGGCGGGCGGAC‑ACCGCAAAAAACCAGCCGCTGACCCTGCTCTAc < 2:7752806/74‑1 (MQ=255) gAACTGGCGCAACACGAGAAAGAAGGAAAGCGGCGGGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACg < 2:5776410/76‑1 (MQ=255) agcTGGCGCCACCCGAGAAAAAAGAAAGGGGGGTGGCCGCC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACgc < 2:3000187/74‑1 (MQ=255) aaCTGGCGCAACAGGAGCAAGAAGGAAAGCGCCTGGCGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACgc < 1:6326757/76‑1 (MQ=255) aaCTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACgc < 1:1657328/76‑1 (MQ=255) aaCTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACgc < 1:7469120/76‑1 (MQ=255) cgggCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACgcag < 1:2816847/74‑1 (MQ=255) tGGCGCAACACGAGCAAGAAGAAAAGCGGCGGGCGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACgcagc < 2:5658659/76‑1 (MQ=255) tGGCCCAACACGAGAAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACgcagc < 1:3810073/76‑1 (MQ=255) gggCGCAACGCGAGAAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACgcagc < 1:5476475/75‑1 (MQ=255) ggCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACgcagca < 1:2079171/76‑1 (MQ=255) gcgcAACACGAGAAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCaa < 1:6729351/76‑1 (MQ=255) cgcAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCaaa < 1:3576024/76‑1 (MQ=255) gcAACAGGAGAAAGAAGGAAAGCGGCGGGCGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCaaaa < 2:23758/76‑1 (MQ=255) cAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCaaaaa < 1:5469768/76‑1 (MQ=255) cAACACGAGAAAGAAGGAAAGCGGCGGGCGAAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCaaaaa < 2:2111783/76‑1 (MQ=255) aaaCACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCaaaaa < 1:5106136/75‑1 (MQ=255) aaCAGGAGCAGAAAGGAAGGCGGCTGGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCaaaaaa < 2:2509700/76‑1 (MQ=255) acacGAGAAAGAAGGAAAGGGGCTGGCGGAC‑ACCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAac < 1:5927221/76‑1 (MQ=255) acacGAGAAAAAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAac < 2:3063922/76‑1 (MQ=255) cacGAGCAAGAAGGAAAGCGGCTGGGGGGT‑ATTGCCCAAAAAAAGCCGCCGGCCCCCCTCTCCCCCGCcaaaaaaa > 1:7242004/1‑74 (MQ=255) cacGAGAAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAaca < 1:4313582/76‑1 (MQ=255) cacGACAAAGAAGAAAAGCGGCTGGCGGCC‑ACCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAaca < 1:4460469/76‑1 (MQ=255) gagAGCAAGAAGGAAAGCGGCTGGGGAAC‑ACCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAacac < 2:389754/74‑1 (MQ=255) cGAGCAAGAAGAAAGGCGGCTGGCGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcacc < 2:2757459/76‑1 (MQ=255) cGAGAAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAAcacc < 1:999516/76‑1 (MQ=255) gCAAGAAGGAAAGCGGCTGGCGGCC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACg < 1:5764476/76‑1 (MQ=255) gCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACg < 2:2235548/76‑1 (MQ=255) cAAGAAGGAAAGCGGCTGGGGGGG‑AACGCCCAAAAAAAACCGCTGAACCCGCTCTACCCCGCAAAAAAaaacacac > 1:5087258/1‑74 (MQ=255) aagaagAAAAGCGGCTGGCGGAC‑ATCCCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCa < 1:5035650/76‑1 (MQ=255) agatgGAAAGGTGCTGGCGGAC‑GTCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAg < 1:4387404/76‑1 (MQ=255) gaagaaAAGCGGGTGGCGAAC‑ACCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGa < 2:4890829/76‑1 (MQ=255) agggAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGaa < 2:7781694/74‑1 (MQ=255) aagGAAACCGGCGGGCGGAC‑ATCGCAAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGaa < 1:5330502/76‑1 (MQ=255) agGAAAGCGGCTGGGGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAAc < 2:488623/76‑1 (MQ=255) aaaGGGGCGGGCGAAC‑ACCCCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCAt < 2:2371626/76‑1 (MQ=255) aaaGCGGCGGGCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCAt < 1:6856248/76‑1 (MQ=255) aGCGGCTGGCGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgc < 2:6507687/76‑1 (MQ=255) gcggcgGGCGCAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATgcg < 2:245834/76‑1 (MQ=255) gcgggCGCAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTg < 2:5311414/73‑1 (MQ=255) cTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTgg > 1:7561404/1‑76 (MQ=255) cTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTgg > 1:7289819/1‑76 (MQ=255) tGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGt > 2:7554755/1‑76 (MQ=255) tGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAACAAAAAACACCACGCAGAACCATGCGCTGGt > 2:6252722/1‑76 (MQ=255) tGGCGGAC‑ACCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGt < 1:2138753/76‑1 (MQ=255) ggCGGAC‑CCCACCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTg < 2:6602335/76‑1 (MQ=255) ggCGGAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTg < 2:4351923/76‑1 (MQ=255) ggCGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTg < 2:3632128/76‑1 (MQ=255) gagAC‑TTCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt < 2:7680179/74‑1 (MQ=255) cGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt > 1:6645250/1‑76 (MQ=255) cGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt > 2:402689/1‑76 (MQ=255) cGGAC‑ATCGCCAAAAAACAGCCGCTGACACTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGAGCt > 2:5941982/1‑76 (MQ=255) cGGAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCt < 2:3246265/76‑1 (MQ=255) ggAC‑ATCGCAAAAAACCAGCCGCTGACCCTGCTCTACGTAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTg < 2:2939303/76‑1 (MQ=255) gAC‑ATCGCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTgg < 1:4288492/76‑1 (MQ=255) c‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGcc > 2:119209/1‑76 (MQ=255) c‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGcc > 2:4291225/1‑76 (MQ=255) accGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCg < 1:2045987/74‑1 (MQ=255) tCGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGa < 1:3078184/76‑1 (MQ=255) cGCAAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGAc < 1:2842733/76‑1 (MQ=255) gcaaaaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACt < 1:5993989/73‑1 (MQ=255) gcaaaaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACt < 2:2926827/73‑1 (MQ=255) gaaaaaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACt < 1:6535675/73‑1 (MQ=255) gCCAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACt < 1:6285402/76‑1 (MQ=255) gCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACt > 2:3570750/1‑76 (MQ=255) ccaaaaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACt < 1:5302606/73‑1 (MQ=255) ccaaaaaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACt < 1:4664089/73‑1 (MQ=255) ccAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg < 1:7072892/76‑1 (MQ=255) ccAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg > 2:7736600/1‑76 (MQ=255) ccAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTg > 2:5659738/1‑76 (MQ=255) cAAAAACCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTgg < 1:4389059/76‑1 (MQ=255) cAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTgg > 1:2383409/1‑76 (MQ=255) aaaaaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGc > 1:940322/1‑76 (MQ=255) aaacCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTa < 1:5636300/76‑1 (MQ=255) aaaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTAc > 1:172480/1‑76 (MQ=255) aaCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGATGCTGGCCGACTTGCTaag > 2:1937359/1‑74 (MQ=255) aCAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTAAGt > 2:2824474/1‑76 (MQ=255) | CTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGAC‑ATCGCCAAAAAACAGCCGCTGACCCTGCTCTACGCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGT > REL606/1854326‑1854476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |