| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,676,636 | 0 | T | G | 37.0% | 428.0 / 96.5 | 238 | V149G (GTG→GGG) | smpB | SsrA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (14/136); new base G (87/1); total (101/137) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 268 total aligned reads displayed.
AAGTGAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCT > REL606/2676561‑2676712 | aGTGAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTg < 1:1569304/76‑1 (MQ=255) gTGAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGgggg > 1:5467577/1‑76 (MQ=255) gTGAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTgg < 2:1470804/76‑1 (MQ=255) tGAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGggggg > 2:5505442/1‑75 (MQ=255) tGAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGa < 1:833814/76‑1 (MQ=255) gAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGAt < 2:2034501/76‑1 (MQ=255) aaaaTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATa < 2:4527796/76‑1 (MQ=255) aaaaTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATa > 1:3515895/1‑76 (MQ=255) aaaTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATaa < 1:1065171/76‑1 (MQ=255) aaTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATaaa < 2:4400880/76‑1 (MQ=255) aaTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATaaa > 1:3976129/1‑76 (MQ=255) aTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAg < 1:4229936/76‑1 (MQ=255) aTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAg < 2:3155327/76‑1 (MQ=255) aTCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAg < 1:3062198/76‑1 (MQ=255) tCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGc < 2:4131761/76‑1 (MQ=255) tCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGc < 1:3394636/76‑1 (MQ=255) tCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGc < 2:3003287/76‑1 (MQ=255) gTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCAccga > 2:2213081/1‑73 (MQ=255) gTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGGAAAGAACGTa > 1:7311744/1‑76 (MQ=255) tCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTAt < 1:1996534/76‑1 (MQ=255) tCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGTAt > 1:2606053/1‑76 (MQ=255) gCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCa < 1:7155487/76‑1 (MQ=255) gCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGAACGAAtta > 1:5663945/1‑74 (MQ=255) ccAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCAt < 2:7797074/76‑1 (MQ=255) ccAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGTATCAt > 1:5368240/1‑76 (MQ=255) cAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATg < 2:6579256/76‑1 (MQ=255) cAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGGATCATg > 1:6827597/1‑76 (MQ=255) aaaGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGAATCATGa > 1:1769328/1‑76 (MQ=255) aaaGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGCACGTATCATGa > 1:6583486/1‑76 (MQ=255) aGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGaaa < 1:1428335/76‑1 (MQ=255) aGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGaaa < 1:6934308/76‑1 (MQ=255) aGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGaaa < 1:7043949/76‑1 (MQ=255) aGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGaaa < 2:1213181/76‑1 (MQ=255) aGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGTATCATGaaa > 1:4821806/1‑76 (MQ=255) ggTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGaaaa < 2:5666727/76‑1 (MQ=255) gTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGGTAAAGCACGTATCATTaaaaa > 2:7558215/1‑76 (MQ=255) gTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGaaaaa < 2:1338069/76‑1 (MQ=255) tAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAAc < 1:1613089/76‑1 (MQ=255) tAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAAc < 1:5597234/76‑1 (MQ=255) tAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAAc < 1:7810621/76‑1 (MQ=255) tAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACAGATCATGaaaaaa > 1:7418269/1‑75 (MQ=255) aaGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGGTAAAGCACCTATCATGAAAAACg > 2:7396209/1‑76 (MQ=255) aaGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGCACGGATCATGAAAAACg > 2:3611344/1‑76 (MQ=255) aGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGc < 1:1224202/76‑1 (MQ=255) aaCAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCa < 2:3214220/76‑1 (MQ=255) aaCAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCa < 2:3329983/76‑1 (MQ=255) aaCAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCa < 1:3311448/76‑1 (MQ=255) aCAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCAc < 2:2371575/76‑1 (MQ=255) cAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCAcc < 1:3182917/76‑1 (MQ=255) gCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGt < 2:610817/76‑1 (MQ=255) cACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGtt < 2:2855970/76‑1 (MQ=255) cACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGtt < 2:1711640/76‑1 (MQ=255) cACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGGATCATGAAAAACGCCCCCCgct > 1:191664/1‑74 (MQ=255) aCGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTa < 2:5739342/76‑1 (MQ=255) aCGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGCACGTATCATGAAAAACGACCACCGTTa > 2:5903815/1‑76 (MQ=255) cGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTaa < 1:3665239/76‑1 (MQ=255) gaaaaaCGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGATAAAGCACGTATCATGAAAAACGCCCACCGTTaaa < 2:1650959/73‑1 (MQ=255) gATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTaaa < 2:4579051/76‑1 (MQ=255) gATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTaaa < 2:4458957/76‑1 (MQ=255) gATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTAACATGAAAAACGCCCACCGTTaaa < 2:229746/76‑1 (MQ=255) gATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAACACGTATCATGAAAAACGCCCACCGTTcaa > 2:4912986/1‑76 (MQ=255) gATAAACGTTCAGATATCAAAGAGCGCGAAGGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTaaa < 2:5411372/76‑1 (MQ=255) aTAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGGTAAAGCACGTATCAAGAAAAAAGCCCACCGTTaaaa > 1:7678760/1‑75 (MQ=255) aTAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAAc < 2:766291/76‑1 (MQ=255) tAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGGACGTATCATGAAAAACGCCCACCCTTAAAcc > 2:7020788/1‑76 (MQ=255) aaaCGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCt < 2:4047981/76‑1 (MQ=255) aaaCGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCt < 1:6459481/76‑1 (MQ=255) aaCGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTg < 1:4635042/76‑1 (MQ=255) aCGTTCAGAAATAAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAATCGCCCACCGTTAAACCTGc < 1:596911/76‑1 (MQ=255) cGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCa < 1:3011403/76‑1 (MQ=255) gTTCAGATATCAAANAGCGCGAATGGCAGGGGGATAAAGCACGTATCATGAAAAAAGCCCACCGTTAAAACTGCAc > 1:4416040/1‑76 (MQ=255) gTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCAc < 1:2581915/76‑1 (MQ=255) gTTCAGATATCAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGTATCATGAAAAACGCCCAACGTTAAAACTGCAc > 2:129153/1‑76 (MQ=255) ttCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACt < 1:4285346/76‑1 (MQ=255) ttCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACt < 1:6118817/76‑1 (MQ=255) ttCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAAACCCCACCGTTAAACCTGCACt > 1:4923457/1‑76 (MQ=255) ttCAGATATCAAAGAGCGCGAATGGCAGGGGAAAAAAGCACGTATCATAAAAAACGCCCACCGTTAAACCTGCACt < 1:6895962/76‑1 (MQ=255) gATATCAAAGAGCGCGAATGGCAGGGGGATAAAACACGTATCATGAAAAACGCCCACCGTTAAACCCACACTCCaa > 2:2583986/1‑76 (MQ=255) atacCAAAGAGCGCGAATGGCAGGTGGATAAAGCAGGTATCATAAAAACCGCCCACCGTTAAACCTGCACTCCAat < 2:373522/76‑1 (MQ=255) tatCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAatt < 2:5269229/76‑1 (MQ=255) tatCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAatt < 2:3909249/76‑1 (MQ=255) tatCAAAGAGCGCGAATGGCAGGGGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAatt > 1:5806983/1‑76 (MQ=255) tCAAAGAGCGCGAATGGCAGGGGGGTAAAACACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAattat > 2:7087953/1‑76 (MQ=255) cAAAGAGCGCGAATGGCAGGGGGGTAAAGCACGAATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAattatt > 2:6784650/1‑76 (MQ=255) cAAAGAGCGCGAATGGCAGGGGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAattatt > 1:487159/1‑76 (MQ=255) aaaGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTg < 2:9034/76‑1 (MQ=255) aaaGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTg < 2:5756288/76‑1 (MQ=255) agCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCa < 1:2325982/76‑1 (MQ=255) aTGGCAGGGGGGTAAAACACGTATCATGAAAAAAGCCCACCGTTAAACCTGCACTCCCATTATTTACCAGTTCCTc > 2:2950328/1‑76 (MQ=255) tGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCa < 1:6356939/76‑1 (MQ=255) tGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCa < 2:1820191/76‑1 (MQ=255) tGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCa < 2:2473763/76‑1 (MQ=255) gggCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCa < 1:188473/75‑1 (MQ=255) ggCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCAc > 2:4765616/1‑76 (MQ=255) cAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACcg > 2:6849354/1‑76 (MQ=255) aGGTGAATAAAGCACGTATCATGAAAACCGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACcgc < 1:3823387/76‑1 (MQ=255) ggTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACcgcg < 2:4837717/76‑1 (MQ=255) gTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCgcgc > 2:62389/1‑76 (MQ=255) gTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCgcgc > 1:6245130/1‑76 (MQ=255) tGGATAAAGCACGTATCATG‑AAAACGCCCACGGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGcct > 2:6077712/1‑76 (MQ=255) | AAGTGAAAATCGGCGTCGCCAAAGGTAAGAAACAGCACGATAAACGTTCAGATATCAAAGAGCGCGAATGGCAGGTGGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCT > REL606/2676561‑2676712 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |