| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,735,608 | 0 | G | C | 45.5% | 34.3 / 19.9 | 33 | A409P (GCC→CCC) | ascF | fused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component |
| Reads supporting (aligned to +/- strand): ref base G (16/2); new base C (0/15); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATGAGCATCG > REL606/2735534‑2735683 | gCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑c < 1:2646571/76‑1 (MQ=255) ccTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑cc < 1:6427424/76‑1 (MQ=255) ccTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑cc < 1:4192894/76‑1 (MQ=255) cTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑cca < 2:6739776/76‑1 (MQ=255) cTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑cca < 1:1419423/76‑1 (MQ=255) cTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑cca < 1:3510221/76‑1 (MQ=255) tggaaCGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑cca‑g < 1:1773740/73‑1 (MQ=255) tGAACCGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑cca‑g < 2:6507858/76‑1 (MQ=255) tGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑cca‑g < 2:4204541/76‑1 (MQ=255) tGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑cca‑g < 1:71400/76‑1 (MQ=255) tGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑cca‑g < 1:2748822/76‑1 (MQ=255) tGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑cca‑g < 1:1235147/76‑1 (MQ=255) tGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑cca‑g < 1:1347919/76‑1 (MQ=255) tGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑cca‑g < 2:3693405/76‑1 (MQ=255) aaCGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTg‑cca‑gcca < 1:5224835/76‑1 (MQ=255) cttttATCGCCAGTTTTATCAGGGGCTTTATTGGCGGCGGGTTTGCCGTTATGGGGGGGCTTC‑CCA‑GCCACTCAAt < 2:5988532/73‑1 (MQ=255) gtttATCGCCAGTCTTATCAGCGGCTTTATTGGCGGCGCGGTTGCCGGTATGGCGGGGCTTC‑CCA‑GCCACTCAATg < 2:1698120/74‑1 (MQ=255) ttATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTC‑CCA‑GCCACTCAATGGc < 2:2593650/76‑1 (MQ=255) ttccGCCATTCTTATAAGCGGCTTTAGTGGCGGGGCGGTTTCGGGGGGGGGGGGGCTGG‑CCA‑GCCACTCAATGGCa < 2:776591/73‑1 (MQ=255) taccGCCATTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTC‑CCA‑GCCACTCAATGGCa < 1:2550483/73‑1 (MQ=255) tATCGCCAGTCTTAGCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTG‑CCA‑GCCACTCAATGGCa < 1:4212410/76‑1 (MQ=255) aTCGCCGGGCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTC‑CCA‑GCCACTCAATGGCAg < 1:6692774/76‑1 (MQ=255) aTCGCCAGTTTTATCAGCCGCTTTATTTGCGGCGGGGTTGCGGGTATGGCGGGTCTTG‑CCA‑GCCACTCAATGGCAg < 1:4545087/76‑1 (MQ=255) tccccAGTTTTACCAGCGTCTTTTTTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTC‑CCA‑GCCACTCAATGGCAgc < 2:6063656/73‑1 (MQ=255) tCGCCAGTCTTACCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTAGGGGGGGGCTTC‑CCA‑GCCACTCAATGGCAgc < 1:2570634/76‑1 (MQ=255) ccgcgAGTCTTACCAGGGGCTTTTTTTGGGGCGGGGTTGCGGGTAGGGGGGGGCTTC‑CCA‑GCCACTCAATGGCAgc < 2:4273306/75‑1 (MQ=255) ctccAGTCTGTTCAGCGGTTTTATTTGCGGCGCGGTTCCGAGTTGGGCGGGCCTTC‑CCA‑GCCACTCAATGGCAgcg < 2:6896566/74‑1 (MQ=255) gTCTTATCAGCGGCTTTATTGGCGGCGCGGTTGCCGGTATGGCGGGGCTTC‑CCA‑GCCACTCAATGGCAGCGCCggg < 1:6570170/76‑1 (MQ=255) tCTTATCAGCTGCTTTTTGTGCGGCGCGTTGGCGGGTAGGGGGGGGCTGC‑CCA‑GCCACTCAATGGCAGCGCCgggg < 1:2119938/76‑1 (MQ=255) cTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGGGTG‑GCAGGCCCCCCAATGGCcggggcgggg > 1:5306374/1‑76 (MQ=255) ttATCAGCGGCTTTATTGGCGGCGCGGTGGCCGGTAGGGCGGGGCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCt < 1:1425274/76‑1 (MQ=255) tATCAGCGGCTTTATTGGCGGCGCGGTTGCCGGTATGGCGGGGCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTa < 1:3278162/76‑1 (MQ=255) tccgCGGTTTTATTTGCGGCGGGGTTGGCGGTATGGCGGCGCTCC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTAtt < 1:4484403/73‑1 (MQ=255) cAGCGTTTTTATTTGCGGCGCGGTGGCGGGTAGGGCGGGGCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTAttt < 2:4680904/76‑1 (MQ=255) aGCGGTTTTTTTGGCGGCGCGGTTGCCGGTATGGCGGGGCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTa < 1:658557/76‑1 (MQ=255) gtggCTTTTTTGGCGGCGCGGTTGCCGGTGGGGCGGGGCTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTAc < 2:5861890/74‑1 (MQ=255) gctgCTTTATTTGCGGCGGGGTTGCGGGTAGGGGGGGGCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTAc < 2:6239019/73‑1 (MQ=255) gCGGTTTTATTGGCGGCGGGGTGGCGGGTAGGGGGGGGCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTAc < 1:890221/76‑1 (MQ=255) cGGTTTTTTTTGGGCCGGGGTGGCGGGTAGGGCGGGGCTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTAcc < 2:2620496/76‑1 (MQ=255) cGGTTTTTTTGGGGGCGGGGTTGCCGGTGTGGCGGGGCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTAcc < 1:6324976/76‑1 (MQ=255) ggttttATGGGCGGCGCGGTGGCGGGTAGGGGGGGGCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCa < 2:457818/73‑1 (MQ=255) gCTTTATTGGCGGCGCGGTTGCCGGTATGGCGGGGCTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAg < 1:5746961/76‑1 (MQ=255) ttttttGGCGGCGCGTTTGCCGGTATGGCGGGCCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCg < 1:4018520/76‑1 (MQ=255) ttATTGGCGGCTAGATTCCCGGGATGGCGGGCCTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGt < 2:4334639/76‑1 (MQ=255) tATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTg < 1:5350933/76‑1 (MQ=255) tATGTGCGGCGCTGTTCCCGGTATGGCGGGGCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTg < 2:1251968/76‑1 (MQ=255) ttcgcggggCGTTGGCGGTTAGGGGGGGGTTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCa < 2:426442/73‑1 (MQ=255) ttGCGCCGCGTTTGCGGGTAGGGCGGGGCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCaa < 2:1705203/76‑1 (MQ=255) gtGCGGCGGTGTTGCGGGTAGGGGGGGCTTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCaa < 2:6921235/75‑1 (MQ=255) ggggcgCGGTGGCGGGTAGGGGGGGGCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAAtt < 1:2998331/74‑1 (MQ=255) gcggcgGTGTTGCGGGTAGGGCGGGGCTCC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAAtt < 1:127542/76‑1 (MQ=255) cgacgcgGTTGCCGGTATGGGGGGGTTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAAttc < 2:2808229/73‑1 (MQ=255) ggcgGGGTTGCGGGTAGGGGGGGGCTGG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAAttct < 1:2537944/76‑1 (MQ=255) gcggGGTTGCGGGTGGGGCGGGGCTCC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAAttctt < 1:5471076/76‑1 (MQ=255) gtttGCGGGTGGGGGGGGGCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGAt < 2:4621904/74‑1 (MQ=255) ggTTGCGGGTGGGGGGGGCCTCC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGAt < 1:6904913/76‑1 (MQ=255) ggTTGCCGGTATGGCGGGCCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGAt < 1:1919690/76‑1 (MQ=255) ggTGGCCGGTGTGGCGGGCATGG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGAt < 2:7684236/76‑1 (MQ=255) gcgggTGTGGCGGGCCTAC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCgg < 1:104528/73‑1 (MQ=255) gTATGGGGGGGCTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGaa < 1:3775242/76‑1 (MQ=255) gTATGGCGGGGCTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTTTTTTCCAGCGGGCAATTCTTCGATCCGGCgga > 1:1233241/1‑74 (MQ=255) gTATGGCGGGGCTTG‑CCA‑GCCACTCAAAGGCAGCGCCGGGGGCCTTTTTCCGCGGGCAAATTTTCTATCCcgcgga > 2:3182756/1‑74 (MQ=255) gTAGGGGGGGCCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGaa < 1:4349568/76‑1 (MQ=255) ggggggtCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCa < 1:447076/73‑1 (MQ=255) ggCGGGGCTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGGATCCa > 1:5264157/1‑76 (MQ=255) gCGGGGCTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTAACAGCGTGCAATTCTTCGATCCGGCGAATCCaa > 1:7702079/1‑76 (MQ=255) cGGGGCTTG‑CCA‑GCCACTCAATGGCAGGGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCCAt > 2:1258461/1‑76 (MQ=255) cGGGGCTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCCGCGAATCCaca > 2:5082554/1‑74 (MQ=255) ggggCTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCCGCGTGCAATTCTTCGATCCCGCGGATCCAATg > 2:5742292/1‑76 (MQ=255) ggggCTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCCGCGTGCAATTCTTCGATCCCGCGAATCCCATg > 2:259283/1‑76 (MQ=255) gggCTTGCCCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATg < 1:1430561/76‑1 (MQ=255) gggCTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATGa < 1:4571381/76‑1 (MQ=255) gggCCTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATGa < 2:7739045/76‑1 (MQ=255) ggccTTC‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATGa < 2:5654064/73‑1 (MQ=255) ggCTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAACGTGCAATTCTTCGATCCGGCGAATCCAATGAg > 2:3899806/1‑76 (MQ=255) cTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATGAGCa > 2:193853/1‑76 (MQ=255) cTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATGAGCa > 1:4087326/1‑76 (MQ=255) cTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATGAGCa > 1:2447663/1‑76 (MQ=255) cTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATGAGCa > 2:596446/1‑76 (MQ=255) ttG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATGAGCAt > 1:936781/1‑76 (MQ=255) ttG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATGAGCAt > 2:888088/1‑76 (MQ=255) tG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATGAGCATc > 2:2643225/1‑76 (MQ=255) tG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATGAGCATc > 2:7432928/1‑76 (MQ=255) g‑cca‑gccaCTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATGAGCATCg > 2:91264/1‑76 (MQ=255) | GCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGCTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTG‑CCA‑GCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAATTCTTCGATCCGGCGAATCCAATGAGCATCG > REL606/2735534‑2735683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |