| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,180,249 | 0 | T | G | 42.9% | 223.9 / 48.8 | 133 | G24G (GGT→GGG) | yjaH | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (21/55); new base G (57/0); total (78/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.16e-20 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 262 total aligned reads displayed.
GGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAG > REL606/4180174‑4180324 | ggCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGt < 1:5286569/76‑1 (MQ=255) aGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTgcg < 2:7128926/76‑1 (MQ=255) tGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGGGCt > 1:1549878/1‑76 (MQ=255) aaCAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGt > 1:4419348/1‑76 (MQ=255) aCAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGtt < 2:5571063/76‑1 (MQ=255) cAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGttt < 2:204773/76‑1 (MQ=255) cAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGttt < 1:5846416/76‑1 (MQ=255) cAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGgtt > 2:2436287/1‑76 (MQ=255) ttttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTtctc < 1:4519159/76‑1 (MQ=255) ttttAACGAAGGGGCGGGTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGGGGTTtttc > 2:2825649/1‑76 (MQ=255) tttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCa < 2:5670372/76‑1 (MQ=255) tttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGGTtctta > 2:4170936/1‑74 (MQ=255) tttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGGTtctcc > 1:5610701/1‑75 (MQ=255) ttAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCAc < 2:3694328/76‑1 (MQ=255) tAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACt < 2:5630691/76‑1 (MQ=255) tAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACt < 1:1422811/76‑1 (MQ=255) aaCGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTg < 2:4222131/76‑1 (MQ=255) aaCGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTg < 2:340048/76‑1 (MQ=255) aaCGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCCGTTTCTCACTg > 1:5765661/1‑76 (MQ=255) aCGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGc < 1:4668685/76‑1 (MQ=255) aCGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGc > 2:7829498/1‑76 (MQ=255) cGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTctctctc > 2:7013745/1‑73 (MQ=255) cGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCTCTTcc > 2:5146349/1‑76 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCt < 1:1155115/76‑1 (MQ=255) gAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTCTCACTGCCt > 2:6627085/1‑76 (MQ=255) aaGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTg < 2:5026000/76‑1 (MQ=255) aaGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTg > 2:3713729/1‑76 (MQ=255) aaGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTTCCTGCCtc > 1:2446682/1‑75 (MQ=255) aGGGGTGGTTTCACCCCTTTTGTCTTTCTGGTGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTTACTGCCTGt > 1:5171166/1‑76 (MQ=255) aGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGt < 1:2930775/76‑1 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTa < 2:2139497/76‑1 (MQ=255) ggggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTTACTGCCCGTa > 2:3799433/1‑76 (MQ=255) gggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAg < 2:4130091/76‑1 (MQ=255) gggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAg < 1:1730321/76‑1 (MQ=255) gggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAg < 1:5879075/76‑1 (MQ=255) gggTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACCGCCTGTAg > 2:1752078/1‑76 (MQ=255) ggtggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGc > 1:1931625/1‑76 (MQ=255) gtggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGGTGTTTTTCACTGCCTGTAGcc > 1:3940783/1‑76 (MQ=255) gtggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGGTGTTTCTTACTGCCCTTAGcc > 1:5568552/1‑76 (MQ=255) gtggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCTGTAggc > 1:4830456/1‑74 (MQ=255) gtggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACCGCCTTTAggc > 2:489486/1‑74 (MQ=255) ggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCCCTGCCTGTAGCcac > 1:7330220/1‑76 (MQ=255) ggtTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGcaca > 2:5908210/1‑73 (MQ=255) tttCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCTCTGCCTGTAGCCCCaa > 1:2156198/1‑76 (MQ=255) tttCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACaa > 1:3066686/1‑76 (MQ=255) tttCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCCGTTTCTTACTGACTGTAGCCACaa > 1:6100865/1‑76 (MQ=255) ttCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTTTCACTTCCTGTAGCCACAAc > 1:6524915/1‑76 (MQ=255) tccccctTTTTGTTTTTCTGGCGTCGATCATTGATGGTGGCGGGTGCGTTGTTTCTCCCTGCTTTTAGCCACAAct < 2:271639/73‑1 (MQ=255) tccccctTTTTGTTTTGCGGGCGTCAATCTTTGATGCTGGCAGGTGCTTTTTTTCTCCCTGCTTTTAGCCACAAct < 2:4540045/73‑1 (MQ=255) ccACCCTTTTTGTTTTTCTGGCGTCTTTCATTGATGCTGGCAGGGCCGCTTTTTCTCCCTCCTTTTAGCCACAAct < 2:6470471/75‑1 (MQ=255) cACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCTGTAGCCCCCActc > 2:6765298/1‑76 (MQ=255) aCCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCCGTAGCCACCActct > 2:1611763/1‑76 (MQ=255) aCCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCCGTTTCTCCCTCCCCGTAGCCACAActcc > 2:4634482/1‑75 (MQ=255) ccccTTTTGTTTTTGTGGCGTCGATCATTGATGCTGGCAGGTGCGTTTTTTCCCCCTGCCTTTAGCCACAACTCtt < 2:5771553/76‑1 (MQ=255) cccTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTc < 1:1015625/76‑1 (MQ=255) cccTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGCTTCTCACTGGCAACTGCCACAACACttg > 2:3194971/1‑75 (MQ=255) ccTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTTTCTCTGCCTTTAGCCACCACTCTTCa > 1:4723899/1‑76 (MQ=255) ccTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCTGTAGCCACAACTCTTCa > 2:6258454/1‑76 (MQ=255) ccTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACCACTCTTCa > 2:3027826/1‑76 (MQ=255) cTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCGGTTTTTCCCTGCCTGTTGCCACAACTCTTcca > 1:3267964/1‑74 (MQ=255) ttttTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCCCTGCCTTTAGCCACAACTCTTCACtt < 2:4261640/76‑1 (MQ=255) tttGTCTTTCTGGCGTCGATCATTGATGCTTGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACtt > 1:136785/1‑76 (MQ=255) ttGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTTTCCCTGCCTGTAGGCACAACTCTTCCCTtc > 1:4179894/1‑76 (MQ=255) tGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGGCCTGTTTTTCCCTGCCTGGAAGCACAACTCTTCACTtcc > 1:3589790/1‑76 (MQ=255) tGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTTTCACTGCCTGTAGCCACAACTCTTCACTtcc > 1:7067446/1‑76 (MQ=255) gTCTTTCAGGCGTCGATCATTGATGCTGGCAGGGGGGCTGTTTTTCACTGCCTGTAGACACAACTCTTCACTtcct > 2:858677/1‑76 (MQ=255) tCTTTCTGGCGTCTATCATTGATGCTGGCAGGTGCGCTGTTTCTCCCTGCCTGTAGCCACAACTCTTCACTtcctc < 1:6864874/76‑1 (MQ=255) tCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGGTGTTTATCACCCCCTGTAGCCACCACCCCTCACTtcccc > 2:7769144/1‑74 (MQ=255) tCTTTCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAAATCTTCACTttctc > 2:4764490/1‑76 (MQ=255) tttCTGGCGTCGATCATTGATGCTGGCAGGGGCGCGGTTTGTCTCTGCCCGTAGCCCCTACTTTTTCTTTCCTccc > 2:1471827/1‑76 (MQ=255) ttCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCCCTTCCTCCCt > 1:4092003/1‑76 (MQ=255) ttCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCt > 2:2287073/1‑76 (MQ=255) ttCTGGCGTCGATCATTGATGCTGGCAGGGGCGCTGGTTCACCCTGCCTGTAGTCACCACGCATCCCCTCCTCCCt > 2:6923688/1‑76 (MQ=255) tcgggCGTCGATTATTGTTGCTGGCAGGTTCGTGGTTTCCCCCGGCCTTTAGCCACAACTCTTCACTTCCTCCCtt < 2:2347210/73‑1 (MQ=255) tcgggCGTCATTCATTCATGTTGGCAGGTGCCCTGTTCCTCCCTGCCTGTAGCCACAACTCTTCACTTCCTCCCtt < 2:1297166/73‑1 (MQ=255) tCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCCCTGCCTTTAGCCACAACTCTTCACTTCCTCCCtt < 1:2662266/76‑1 (MQ=255) tCTGGCGTCGATCATTGATGCTGGCAGGTGCGCCGTTTCTCACTGCCTGTAGCCCCAAATCTTCACTTTCTTCCtt > 2:4060242/1‑76 (MQ=255) tGGCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCCCTGCCTGGAGCCACCAATCCTCACTTCCTCCCTTTa > 1:7547441/1‑76 (MQ=255) tGGCGTCGATCATTGATGCGGGCTGGTGCGTTTTTTCGCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTa < 2:7056287/76‑1 (MQ=255) ggCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCttttc > 1:4354814/1‑74 (MQ=255) ggCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAAATCTTCAATTCCTCCCTTTAc > 1:3452071/1‑76 (MQ=255) ggCGTCGATCATTGAGGCTGGCAGGTGCGCTTTTTCTCCCTGCCTTTAGCCACAACTCTTCACTTCCTCCCTTTAc < 1:6261795/76‑1 (MQ=255) gCGTCGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTAcc > 1:6657431/1‑76 (MQ=255) tCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGcc < 1:3852001/76‑1 (MQ=255) cGATCATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCCCTTCCTCCCTTTTCCGCCa > 2:1046745/1‑76 (MQ=255) aTCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGt > 2:5853695/1‑76 (MQ=255) cATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCCCCCGTgg > 1:1596408/1‑76 (MQ=255) cATTGATGCTGGCAGGGGCGCTGTTTCTCACTGCCTGTAGCCACAACCCTTCACTTCCTCCCTTTACCGCCAGCgg > 1:895416/1‑76 (MQ=255) ttGATGCTGGCAGGGGCGCTGTTTCTCACTGGCTGTAGACACAACCCTTCCCTTTCTCCCTTTCATGCCAGTGGAt > 2:1977863/1‑76 (MQ=255) tGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGa > 1:6339919/1‑76 (MQ=255) ggggTGCTCTTTTTCTCTCTGCTTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGa < 2:7253732/74‑1 (MQ=255) cAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGa > 2:6834776/1‑76 (MQ=255) cAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGa > 1:2395466/1‑76 (MQ=255) aGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAc > 1:5469117/1‑76 (MQ=255) aGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAc > 1:7798520/1‑76 (MQ=255) aGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGAc > 1:1518223/1‑76 (MQ=255) aGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTCGCTTTGCTGAAGAc > 2:7389263/1‑76 (MQ=255) gTGCGTTTTTTCCCCCTCCTTTTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCa < 2:2210572/76‑1 (MQ=255) gTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCa > 2:4439836/1‑76 (MQ=255) tGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAg > 1:6919864/1‑76 (MQ=255) | GGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGGCAGGTGCGCTGTTTCTCACTGCCTGTAGCCACAACTCTTCACTTCCTCCCTTTACCGCCAGTGGATTTGCTGAAGACCAG > REL606/4180174‑4180324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |