| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 925,639 | 0 | T | G | 36.2% | 216.9 / 33.4 | 116 | K121N (AAA→AAC) | ltaE | L‑allo‑threonine aldolase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (22/52); new base G (42/0); total (64/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 165 total aligned reads displayed.
ACGCGGCAGCATTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTGCGGCTGAATACTG > REL606/925564‑925714 | gcgGCAGCATTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAAAGGATATCGTCGGGgtt > 2:849672/1‑76 (MQ=255) cgGCAGCATTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTg < 2:2071169/76‑1 (MQ=255) cgGCAGCATTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTg < 1:2966061/76‑1 (MQ=255) ggCAGCATTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGa < 2:6525268/76‑1 (MQ=255) cagcaTTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGAtt < 2:1847855/76‑1 (MQ=255) agcaTTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGAttt < 1:6137411/76‑1 (MQ=255) agcaTTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGGttt > 1:417422/1‑76 (MQ=255) gcaTTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATTGATATCGTCGGGTTTGAtttt < 2:7794748/76‑1 (MQ=255) gcaTTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGAtttt > 1:7594724/1‑76 (MQ=255) caTTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGGttttt > 1:7438816/1‑75 (MQ=255) aTTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGAttttta > 2:4613418/1‑74 (MQ=255) tttGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATc < 2:2743131/76‑1 (MQ=255) tttGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATc < 2:5657065/76‑1 (MQ=255) ttGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGGTTTTTATCg > 2:3500799/1‑76 (MQ=255) gCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGGTTTTCATCTcc > 2:448445/1‑76 (MQ=255) gCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTTCTCGcc > 2:3254142/1‑76 (MQ=255) ccGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCa < 1:6965521/76‑1 (MQ=255) ccGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTTATCGccc > 2:4357986/1‑75 (MQ=255) ccGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTCATCGccc > 2:7092937/1‑75 (MQ=255) cGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTTATTGCCAc > 2:2979498/1‑76 (MQ=255) gTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGGTTTTTTTCTCCACt > 1:4254163/1‑76 (MQ=255) gTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTTATCGCCACt > 1:3276580/1‑76 (MQ=255) ttGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCCCtt > 2:7694718/1‑76 (MQ=255) ttGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTTATTTTCCTCGCCACtt > 2:681756/1‑76 (MQ=255) ttGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTTATTGGCACtt > 2:6038937/1‑76 (MQ=255) ttGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTTATTGCCACtt > 2:355315/1‑76 (MQ=255) tgtgGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACttt < 1:6642718/76‑1 (MQ=255) tgtgGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTTATCGCCACttt > 2:3290365/1‑76 (MQ=255) tgtgGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTCATCGCCCCttt > 2:1222871/1‑76 (MQ=255) gtgGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTa < 2:6975891/76‑1 (MQ=255) tgGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGTTTTTTATTGCCACTTTAt > 1:454582/1‑76 (MQ=255) tgGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTCATCGGCCCTCTAt > 2:6306856/1‑76 (MQ=255) gggTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATc < 2:5054395/76‑1 (MQ=255) gggTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTCATCGCCACTTTATc > 1:2265191/1‑76 (MQ=255) tgtTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGTCACTTTATCCAg < 1:1237281/76‑1 (MQ=255) gtTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGc < 2:3932536/76‑1 (MQ=255) ttttCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCg < 2:7215165/76‑1 (MQ=255) tttCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTTATCGCCACTTTATCCAGCgg > 1:5343162/1‑76 (MQ=255) ttCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGt < 1:5946462/76‑1 (MQ=255) ttCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGt < 2:1720491/76‑1 (MQ=255) ttCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGt < 1:3664355/76‑1 (MQ=255) tCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCggga > 1:3813665/1‑74 (MQ=255) tCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTCATCGCCCCTTTATCCAGCggga > 1:7196800/1‑74 (MQ=255) cAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTTATCGCCACTTTATCCAGCGGTaac > 2:1570224/1‑74 (MQ=255) aGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTTGTTTTCATCGCCACTTTATCCAGCGGTAGCg > 1:953527/1‑76 (MQ=255) aGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTCATTGCCACTTTATCCAGCCGGAGCg > 2:7035100/1‑76 (MQ=255) gACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGt < 2:7511338/76‑1 (MQ=255) cTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGGTTTTTATCGCCACTTTATCCAGCGGGAGGGTGc > 1:5340179/1‑76 (MQ=255) cTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTCATCGCCCCTTTATCCAGCGGGAGCGTGc > 1:1116843/1‑76 (MQ=255) tGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGcc > 2:1299716/1‑76 (MQ=255) gAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTTATCGCCCCTTTTTCCAACGGGAGGGTGGCg > 1:4433111/1‑76 (MQ=255) aGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTTATCGGCACTTTATCCAGCCGGCGCGTTCCgc > 2:7520887/1‑75 (MQ=255) tAATTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGCTTTTTATCGCCACTTTATCCAGCGGGAGCGTGCCGTCg > 2:6731879/1‑76 (MQ=255) aaTTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCgg < 1:3455493/76‑1 (MQ=255) aTTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGGTTTTCATCGCCACTTTATCCAGCGGTAGGGTGCCCGCGGc > 2:6550280/1‑76 (MQ=255) aTTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGc < 2:5197097/76‑1 (MQ=255) aTTTGGTGCGGGCGAAATGGATATCGTCGGGGTTTATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCggg > 2:4092882/1‑75 (MQ=255) aTTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTTATCGCCCCTTTATCCAGCGGTAGCGTGCCGTCggg > 1:688236/1‑75 (MQ=255) aTTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTTATCGCCACTTTATTCAGCGGTAGCGTGCCGTCggg > 2:6147469/1‑75 (MQ=255) aTTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTTATCGCCACTTTATCCAGCGGTAGCGTGCCCTCggg > 2:5392498/1‑75 (MQ=255) aTTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTCCTCGCCAATTTCTTCAACGGTAGCGCGCCGTCGGc > 2:7353533/1‑76 (MQ=255) aTTTGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTCATCGCCACTTTATCCAGCGGGAGCGTGCCGTCggg > 1:5294440/1‑75 (MQ=255) tttGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCa < 2:7859309/76‑1 (MQ=255) ttGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACCTTATCCAGCGGGAGCGTGCCGTCGGcgg > 1:5784548/1‑74 (MQ=255) tGGTGCGGGCGAAATGGATATCGTCGGGGTTGATTTTTATCGCCCCTTTATCCAGCGGTAACGTGTCGTCGGGAGc > 2:4390234/1‑76 (MQ=255) gCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCcgcg < 1:5811575/76‑1 (MQ=255) gCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCcgcg < 1:1644310/76‑1 (MQ=255) gggCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTc < 1:647959/76‑1 (MQ=255) gggCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTc < 1:4666488/76‑1 (MQ=255) ggCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCg > 1:5621542/1‑76 (MQ=255) gagAAATGGATATCGTCGGTTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGa < 2:4423456/74‑1 (MQ=255) ggAAAGGAATATCGTCGGGTTTGATTTCCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGAt < 2:743105/75‑1 (MQ=255) ctaaaTGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGAt < 1:3821869/74‑1 (MQ=255) gAAATGGATATCGTCGGTTTTAATTTTCATCGCCCCTTTACCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATg < 2:407861/76‑1 (MQ=255) gAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATg < 1:1906997/76‑1 (MQ=255) gAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATg < 1:4157063/76‑1 (MQ=255) aaaTGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATgg < 1:2040590/76‑1 (MQ=255) aaTGGATATCGTCGGGTTTGATTTTTATCGCCACTTTATCCAGCGGTAGCGGGCCGTCGGGAGGCGCCGCGGTggg > 2:7306841/1‑76 (MQ=255) aTGGATATCGTCGGTTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGt < 1:4420585/76‑1 (MQ=255) tGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGtt < 1:849672/76‑1 (MQ=255) gtatatCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTg < 1:3409903/74‑1 (MQ=255) gttatCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTGc < 1:5043956/74‑1 (MQ=255) gATATCGTCGGTTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTGc < 1:7190349/76‑1 (MQ=255) tcgtcgGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGGCGGTGGGGTGCgggt > 1:5019314/1‑74 (MQ=255) cgtcgGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGGAGGCCCGGCGGtggggtgggggcg > 2:1092210/1‑73 (MQ=255) cgtcgGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGCCGATGGGTTGCGGcgg > 2:1903077/1‑74 (MQ=255) cgtcgGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCCCGTCGCTGGGGTGCGGCtt > 2:4669566/1‑75 (MQ=255) tcgGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGTCGCCGCGTCGCTGGGTGGCGGCTgga > 1:281287/1‑74 (MQ=255) tcgGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTGCGGCTGaa < 1:100225/76‑1 (MQ=255) ggtgTTTGATTTTCATCGCCCCTTTACCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTGCGGCTGAAt < 2:2002635/73‑1 (MQ=255) ggggTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTGCGGCTGAAt < 2:5731450/75‑1 (MQ=255) cgtgtttgtttTCCATCGCCGCTATTTCCAGTGTGACCGTGCAGTCGCCAGCCGCGTCGATGGGTTGCGGCTGAAt < 2:2247693/73‑1 (MQ=255) cgGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTGGGGCTGAAt > 1:7337972/1‑76 (MQ=255) cgGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTGCGGCTGAAt < 1:3930450/76‑1 (MQ=255) cgGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGAGTTGCGGCTGaaa > 1:5830008/1‑75 (MQ=255) gggTTTGTTTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTGCGGCTGAATa < 2:6329422/76‑1 (MQ=255) gtttgtttTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTGCGGCTGAATACt < 2:2403541/76‑1 (MQ=255) gTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGAAGGGGTGCGGCTGGAAACt > 1:155445/1‑76 (MQ=255) tttGTTTTCCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTGCGGCTGAATACTg < 2:6507663/76‑1 (MQ=255) tttGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTGCGGCTGGATACTg > 1:458875/1‑76 (MQ=255) | ACGCGGCAGCATTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTGCGGCTGAATACTG > REL606/925564‑925714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |