BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,145,707	C→T	intergenic (+38/‑157)	dnaG → / → rpoD	DNA primase/RNA polymerase sigma factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,145,707	0	C	T	100.0%	688.2 / NA	175	intergenic (+38/‑157)	dnaG/rpoD	DNA primase/RNA polymerase sigma factor
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (138/37); total (138/37)

Only 100 of 234 total aligned reads displayed.

CTCTGGACATTAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCC‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACC  >  REL606/3145634‑3145780

ctctGGACATTAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGcct‑cc                                                                         >  2:241830/1‑76 (MQ=255)

ctctGGACATTAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGcct‑cc                                                                         >  2:3737506/1‑76 (MQ=255)

 tctGGACATTAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCg                                                                        <  2:2671889/76‑1 (MQ=255)

  ctGGACATTAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGGCT‑CCGa                                                                       >  1:4551905/1‑76 (MQ=255)

  ctGGACATTAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGa                                                                       >  1:7817029/1‑76 (MQ=255)

  ctGGACATTAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGa                                                                       >  2:3591786/1‑76 (MQ=255)

  ctGGACATTAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGa                                                                       >  1:2790342/1‑76 (MQ=255)

   tGGACCTTAAACC‑AGCAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAACCCT‑CCGAc                                                                      <  2:5725597/76‑1 (MQ=255)

    ggACATTAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCAACa                                                                     <  2:5620088/76‑1 (MQ=255)

      aCATTAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGc                                                                   >  1:7223904/1‑76 (MQ=255)

        ataaaaCC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCGGAAGAGCGATCGGAAAGCCT‑CCGACAGCCg                                                                 <  2:2869628/73‑1 (MQ=255)

         ttAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGc                                                                >  2:1910931/1‑76 (MQ=255)

         ttAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGc                                                                >  2:6693449/1‑76 (MQ=255)

         ttAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGc                                                                >  1:1090502/1‑76 (MQ=255)

         atAAACCAAGGAGCGGGCAAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCAATCGGGAAGCCT‑CCGACAGCCg                                                                 <  2:7304089/75‑1 (MQ=255)

          tAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCa                                                               >  2:3142983/1‑76 (MQ=255)

           aaaCC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCAc                                                              >  1:2657103/1‑76 (MQ=255)

           aaaCC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCAc                                                              >  1:7459627/1‑76 (MQ=255)

           aaaCC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCAc                                                              >  2:7254947/1‑76 (MQ=255)

           aaaCC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCAc                                                              >  2:3499244/1‑76 (MQ=255)

           aaaCC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCAc                                                              <  1:4137979/76‑1 (MQ=255)

            aaCC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACt                                                             <  1:1827609/76‑1 (MQ=255)

             aCC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTg                                                            >  2:4909338/1‑76 (MQ=255)

             aCC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTg                                                            <  1:1257850/76‑1 (MQ=255)

               ccgggAGCGGGCGAAAAATTGATTTAGCGGCTTAGGTGCCGAAGAGCGATGGGGAAGCCT‑CCGACAGCCGCACTga                                                           <  2:6143249/73‑1 (MQ=255)

               c‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTgag                                                          >  2:3249195/1‑76 (MQ=255)

                 aGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTgaga                                                         >  1:3083498/1‑76 (MQ=255)

                 aGGAGCTGGCGAAAAAGTGATTAAACGTCTTAAGTGCCGAAGAGCGATGGGGAAGCCT‑CCGACAGCCGCACTgaga                                                         <  2:1033010/76‑1 (MQ=255)

                    ggCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGc                                                      >  1:6522613/2‑76 (MQ=255)

                    aGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGc                                                      >  1:6049654/1‑76 (MQ=255)

                    aGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGc                                                      <  1:3238201/76‑1 (MQ=255)

                     gcgggCAAAAAAGTGTTTTAACGGCTAAAGTGCCAAAGAGCGATCGGAAAGCCT‑CCGACAGCCGCACTGAGAGGCa                                                     <  2:5334927/73‑1 (MQ=255)

                     gCTGGCGAAAAAGTGATTTAACGGCTTAAGTCCCGAAGGGCGAGCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCa                                                     <  1:4519564/76‑1 (MQ=255)

                      cTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGGGAGGCAg                                                    >  2:2996448/1‑76 (MQ=255)

                      cTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAg                                                    >  1:6661731/1‑76 (MQ=255)

                        ggCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCg                                                  >  1:1910623/1‑76 (MQ=255)

                        ggCAAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCg                                                  <  1:728741/76‑1 (MQ=255)

                        ggCAAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCg                                                  >  1:2053572/1‑76 (MQ=255)

                          cGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGc                                                >  2:4516233/1‑76 (MQ=255)

                          cGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGc                                                >  1:893775/1‑76 (MQ=255)

                          cGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGc                                                >  1:6507339/1‑76 (MQ=255)

                           gAAAAATTATTTTACCGGTTAAAGTCCTGAAGAGGACACGGAAACCCC‑CCGACAGCCGCACTGAGAGGCAGCGGCa                                               <  2:3542966/76‑1 (MQ=255)

                           aaaaaaGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCa                                               <  1:2862279/75‑1 (MQ=255)

                            aaaaaGTGATTTAACGGCTTAAGTGCCGAAGAGCGATGGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCaa                                              <  1:2908427/76‑1 (MQ=255)

                            aaaaaGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCaa                                              >  2:5866253/1‑76 (MQ=255)

                            aaaaaGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCaa                                              >  2:4744111/1‑76 (MQ=255)

                            aaaaaGTAATTAAACGTTTAAGGTGCCAAAGAGC‑ACCGGGAAACCCTCCGACAGCCGCACTGAGAGGCAGCGGCaa                                              <  2:4373269/76‑1 (MQ=255)

                              aaaGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAat                                            >  1:3319902/1‑76 (MQ=255)

                               aaGTGATTTAACGGCTTAAGTGCAGAAGAGCGATGGGAAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAata                                           <  2:5475181/76‑1 (MQ=255)

                                 gTGATTCAACGGCTTAAGTGCGGAAGAGCGATGGGGAACCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAatata                                         <  2:530464/76‑1 (MQ=255)

                                  tattttAACGGTTAAAGGCCCAAAGGGCAACGGGAAACCCC‑CCGCCAGCCGCACTGAGAGGCAGCGGCAAatatat                                        <  2:6977933/73‑1 (MQ=255)

                                  tGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAatatat                                        >  1:1233532/1‑76 (MQ=255)

                                  tGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAatatat                                        >  1:3887105/1‑76 (MQ=255)

                                  tGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAatatat                                        >  2:6849900/1‑76 (MQ=255)

                                   gATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAAtatata                                       >  2:7358199/1‑76 (MQ=255)

                                    aTTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATaa                                      >  1:1488895/1‑76 (MQ=255)

                                     tttAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAg                                     >  2:5056322/1‑76 (MQ=255)

                                     tttAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAg                                     >  1:4398693/1‑76 (MQ=255)

                                      ttAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGt                                    >  2:7542780/1‑76 (MQ=255)

                                      ttAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGt                                    >  1:2559484/1‑76 (MQ=255)

                                       tAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTa                                   >  1:4259171/1‑76 (MQ=255)

                                        aaCGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTAc                                  >  1:1672450/1‑76 (MQ=255)

                                        aaCGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTAc                                  >  2:4423793/1‑76 (MQ=255)

                                         aCGGCTTAAGTTCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACg                                 >  1:7857746/1‑76 (MQ=255)

                                         aCGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACg                                 >  2:3307137/1‑76 (MQ=255)

                                          cGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTAAGc                                >  2:7076302/1‑76 (MQ=255)

                                            gCTTAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAACTATATGAGTACGccc                              >  2:2971721/1‑76 (MQ=255)

                                              ttAAGTGCCGAAGAGCGATGGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTc                            <  2:1847049/76‑1 (MQ=255)

                                              ttAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATACGTACGCCCtg                            >  1:5408290/1‑75 (MQ=255)

                                              ttAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTc                            >  1:4273150/1‑76 (MQ=255)

                                              ttAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTc                            >  2:5824128/1‑76 (MQ=255)

                                              ttAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTc                            >  2:6271426/1‑76 (MQ=255)

                                               tAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCCAATATATAAGTACGCCCTCg                           >  1:6775389/1‑76 (MQ=255)

                                               tAAGTGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCg                           >  2:246353/1‑76 (MQ=255)

                                                 agggCCGAAGAGCAATGGGAAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTa                         <  2:4662316/73‑1 (MQ=255)

                                                 aGTGCCGAAGAGCGAGCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTa                         <  1:5797282/76‑1 (MQ=255)

                                                   tGCCGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAAt                       <  1:3736389/76‑1 (MQ=255)

                                                    tccGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAAtt                      >  2:7739594/2‑76 (MQ=255)

                                                    gCCAAAGAGCGATGGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAAtt                      <  1:5502187/76‑1 (MQ=255)

                                                     ccGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTa                     >  1:5368317/1‑76 (MQ=255)

                                                      cGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTAt                    <  1:1716358/76‑1 (MQ=255)

                                                      cGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTAt                    >  2:2720823/1‑76 (MQ=255)

                                                      cGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTAt                    >  2:2281315/1‑76 (MQ=255)

                                                      cGAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTAt                    >  2:5553312/1‑76 (MQ=255)

                                                       gAAGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATc                   >  2:2157872/1‑76 (MQ=255)

                                                        aaGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCg                  >  1:3711815/1‑76 (MQ=255)

                                                        aaGAGCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCg                  >  1:6036195/1‑76 (MQ=255)

                                                         agagCGATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGt                 >  2:2600173/1‑76 (MQ=255)

                                                         agagCGAGGGGAAACCCC‑CCGCCAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGt                 <  2:1220883/76‑1 (MQ=255)

                                                              gATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCg            >  2:1505432/1‑76 (MQ=255)

                                                              gATCGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCg            >  1:484949/1‑76 (MQ=255)

                                                                tCGGGAAGCCT‑CCGCCAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGt          <  1:6814009/76‑1 (MQ=255)

                                                                ccGGGACGCCC‑CGCCCAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGt          <  2:4041063/75‑1 (MQ=255)

                                                                 cGGGAAGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTa         >  2:3836562/1‑76 (MQ=255)

                                                                  gggAACCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTaa        <  1:4625924/76‑1 (MQ=255)

                                                                    gAACCCC‑CCGCCAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAaac      <  1:2437469/76‑1 (MQ=255)

                                                                     aaGCCT‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAaaca     >  1:5846428/1‑76 (MQ=255)

                                                                      acccc‑cccACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAaacaa    <  2:1320970/74‑1 (MQ=255)

                                                                      aGCCC‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAaacaa    <  1:980152/76‑1 (MQ=255)

                                                                       cccc‑ccGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAaacaac   <  1:4867973/75‑1 (MQ=255)

CTCTGGACATTAAACC‑AGGAGCTGGCGAAAAAGTGATTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCC‑CCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACC  >  REL606/3145634‑3145780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`