BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	649,601	T→C	I401V (ATC→GTC)	mrdA ←	transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	649,601	0	T	C	100.0%	855.6 / NA	196	I401V (ATC→GTC)	mrdA	transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (85/111); total (85/111)

Only 100 of 221 total aligned reads displayed.

CGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACG  >  REL606/649528‑649673

  ttcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACcccc                                                                      >  1:6354770/1‑76 (MQ=255)

   tcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCa                                                                     >  1:7858102/1‑76 (MQ=255)

    cttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCat                                                                    <  1:174392/76‑1 (MQ=255)

    cttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCat                                                                    <  1:2052013/76‑1 (MQ=255)

     ttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCata                                                                   >  2:3329541/1‑76 (MQ=255)

     ttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCata                                                                   >  1:2763437/1‑76 (MQ=255)

      tcCGCCAGTACGATGCCGGTGTAATTACCATAACCGAATTGCCCCATCCATTCGGAGAGGCGATCGACCCCCatat                                                                  <  1:7317793/76‑1 (MQ=255)

      tcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCatat                                                                  <  1:5350200/76‑1 (MQ=255)

      tcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCatat                                                                  <  2:2691970/76‑1 (MQ=255)

      tcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCatat                                                                  <  1:1133076/76‑1 (MQ=255)

       ccGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATc                                                                 >  1:5676890/1‑76 (MQ=255)

        cGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCg                                                                <  1:210254/76‑1 (MQ=255)

         gCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGt                                                               <  2:5978778/76‑1 (MQ=255)

           cAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATCTCGTAg                                                             >  1:3711013/1‑76 (MQ=255)

           cAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAg                                                             <  2:6745405/76‑1 (MQ=255)

           cAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAg                                                             <  1:3935371/76‑1 (MQ=255)

            aGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAgg                                                            >  1:3262818/1‑76 (MQ=255)

                 gATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATc                                                                 <  1:5985766/66‑1 (MQ=255)

                 gATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATc                                                                 >  2:5985766/1‑66 (MQ=255)

                 gATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCAcc                                                       >  1:1803021/1‑76 (MQ=255)

                  aTACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCt                                                      >  2:2612524/1‑76 (MQ=255)

                  aTACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCt                                                      <  2:7231421/76‑1 (MQ=255)

                  aTACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCt                                                      >  1:4376652/1‑76 (MQ=255)

                   tACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTg                                                     >  2:5284336/1‑76 (MQ=255)

                   tACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTg                                                     >  1:1163152/1‑76 (MQ=255)

                    aCCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGa                                                    <  2:5337138/76‑1 (MQ=255)

                     ccGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGAt                                                   <  1:5101345/76‑1 (MQ=255)

                       ggTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATag                                                 <  1:299612/76‑1 (MQ=255)

                       ggTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATag                                                 <  1:1437866/76‑1 (MQ=255)

                       ggTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATag                                                 <  1:594935/76‑1 (MQ=255)

                       ggTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATag                                                 <  2:3552972/76‑1 (MQ=255)

                        gtgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATaga                                                <  1:6821357/76‑1 (MQ=255)

                        gtgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATaga                                                <  2:2092303/76‑1 (MQ=255)

                        gtgtAAGGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATaga                                                <  2:4054415/76‑1 (MQ=255)

                         tgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaa                                               >  2:366608/1‑76 (MQ=255)

                         tgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaa                                               >  2:2553199/1‑76 (MQ=255)

                         tgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTACGCCACCTGATagaa                                               >  1:4299925/1‑76 (MQ=255)

                          gtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaag                                              <  1:3620286/76‑1 (MQ=255)

                          gtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaag                                              >  1:6604704/1‑76 (MQ=255)

                          gtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaag                                              <  1:6422418/76‑1 (MQ=255)

                           tAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaaga                                             <  2:6508622/76‑1 (MQ=255)

                           tAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaaga                                             >  2:101019/1‑76 (MQ=255)

                            aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAgaagaa                                            <  2:4699215/76‑1 (MQ=255)

                            aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAgaagaa                                            <  2:7408085/76‑1 (MQ=255)

                            aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAgaagaa                                            >  2:229862/1‑76 (MQ=255)

                            aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAgaagaa                                            <  1:1026170/76‑1 (MQ=255)

                              tGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAgg                                          >  1:268000/1‑76 (MQ=255)

                               gACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAgga                                         >  2:3907899/1‑75 (MQ=255)

                                aCCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTa                                        <  1:5557810/76‑1 (MQ=255)

                                aCCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTa                                        <  1:4416033/76‑1 (MQ=255)

                                 ccATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTAt                                       <  2:152040/76‑1 (MQ=255)

                                  cATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATc                                      <  2:7825919/76‑1 (MQ=255)

                                   aTAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATcc                                     <  2:578516/76‑1 (MQ=255)

                                   aTAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATcc                                     <  1:4732653/76‑1 (MQ=255)

                                   aTAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATcc                                     <  1:7134680/76‑1 (MQ=255)

                                     aaCCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGc                                   <  1:4127974/76‑1 (MQ=255)

                                     aaCCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGc                                   <  1:3379612/76‑1 (MQ=255)

                                     aaCCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGc                                   >  2:5733412/1‑76 (MQ=255)

                                     aaCCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGc                                   >  1:5467866/1‑76 (MQ=255)

                                       ccGAATTTCCCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAg                                 <  1:696986/76‑1 (MQ=255)

                                       ccGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAg                                 <  2:6876271/76‑1 (MQ=255)

                                        cGAATTTCCCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGa                                <  2:5505249/76‑1 (MQ=255)

                                        cGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGa                                >  2:2775912/1‑76 (MQ=255)

                                         gAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAt                               >  2:1359590/1‑76 (MQ=255)

                                         gAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGGATCCGCAGAt                               >  2:1981383/1‑76 (MQ=255)

                                          aaTTTCCCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAtt                              <  2:6637526/76‑1 (MQ=255)

                                          aaTTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAtt                              <  2:905342/76‑1 (MQ=255)

                                          aaTTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAtt                              >  1:6755951/1‑76 (MQ=255)

                                            tttACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttct                            >  2:5251076/1‑76 (MQ=255)

                                            tttACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAACGTATCCGCAGAttct                            >  2:5759853/1‑76 (MQ=255)

                                             ttACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttctt                           >  1:939578/1‑76 (MQ=255)

                                              tACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttattc                          >  1:7807840/1‑76 (MQ=255)

                                               gcccATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTcc                         <  2:5615089/75‑1 (MQ=255)

                                                 ccatccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAg                       <  2:1318107/76‑1 (MQ=255)

                                                 ccatccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAg                       <  2:4156956/76‑1 (MQ=255)

                                                  catccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGc                      <  2:4985859/76‑1 (MQ=255)

                                                  catccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGc                      >  2:6436960/1‑76 (MQ=255)

                                                  catccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGc                      >  1:4836632/1‑76 (MQ=255)

                                                   atccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCg                     <  1:6212363/76‑1 (MQ=255)

                                                    tccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGa                    >  1:2702192/1‑76 (MQ=255)

                                                     ccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt                   <  1:5769477/76‑1 (MQ=255)

                                                     ccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt                   >  1:2300688/1‑76 (MQ=255)

                                                     ccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt                   >  1:2257509/1‑76 (MQ=255)

                                                     ccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt                   >  1:1266069/1‑76 (MQ=255)

                                                     ccatTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt                   >  2:3986721/1‑76 (MQ=255)

                                                      catTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATc                  >  2:4234554/1‑76 (MQ=255)

                                                        ttCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCtt                <  1:3663088/76‑1 (MQ=255)

                                                         tCGGAGAGGCGATCGCCCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTtg               <  2:2469641/76‑1 (MQ=255)

                                                          cGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTtgt              >  1:5244217/1‑76 (MQ=255)

                                                          cGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTtgt              >  1:3175686/1‑76 (MQ=255)

                                                            gagaGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGa            >  2:4418552/1‑76 (MQ=255)

                                                             agagGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGAc           >  1:426609/1‑76 (MQ=255)

                                                              aagGAGATCGACCACAATATCGTAGGCAACTTGATAGAAGAAGGTATCCGCACATTCTTCCAGCGATCTTGTGACa          <  2:3289363/75‑1 (MQ=255)

                                                                 gCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTc       >  1:7505997/1‑76 (MQ=255)

                                                                   gatcgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAg     >  2:1187601/1‑76 (MQ=255)

                                                                    atcgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGa    <  2:5009761/76‑1 (MQ=255)

                                                                    atcgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGa    <  1:1035629/76‑1 (MQ=255)

                                                                     tcgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGAc   <  2:4745496/76‑1 (MQ=255)

                                                                     tcgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGAc   <  1:695107/76‑1 (MQ=255)

                                                                      cgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACg  >  1:2932444/1‑76 (MQ=255)

CGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACG  >  REL606/649528‑649673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`