BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	711,401	A→G	D58G (GAT→GGT)	ybfA →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	711,401	0	A	G	100.0%	482.1 / NA	108	D58G (GAT→GGT)	ybfA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/87); total (21/87)

Only 100 of 116 total aligned reads displayed.

AAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGA  >  REL606/711328‑711472

aaaGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTc                                                                       <  1:5631888/76‑1 (MQ=255)

aaaGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTc                                                                       <  1:2541040/76‑1 (MQ=255)

 aaGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCa                                                                      <  1:1653888/76‑1 (MQ=255)

 aaGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCa                                                                      <  1:685222/76‑1 (MQ=255)

 aaGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCa                                                                      <  2:5904060/76‑1 (MQ=255)

    aCCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAATCCAGCGATAAACCGGTGTGGATGGGTCAGGc                                                                   <  2:6070611/76‑1 (MQ=255)

     ccGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGcc                                                                  <  2:6436284/76‑1 (MQ=255)

      cgcgCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCg                                                                 <  2:4046192/76‑1 (MQ=255)

      cgcgCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCg                                                                 <  1:6675699/76‑1 (MQ=255)

      cgcgCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCg                                                                 <  2:4688685/76‑1 (MQ=255)

      cgcgCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCg                                                                 <  2:4695026/76‑1 (MQ=255)

       gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGa                                                                <  1:980170/76‑1 (MQ=255)

        cgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGaa                                                               <  2:4352764/76‑1 (MQ=255)

         gcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGaaa                                                              <  2:7820996/76‑1 (MQ=255)

         gcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGaaa                                                              <  1:3074750/76‑1 (MQ=255)

          cccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGaaaa                                                             <  1:7858714/76‑1 (MQ=255)

           ccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAg                                                            <  1:3586090/76‑1 (MQ=255)

           ccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAg                                                            <  1:2609200/76‑1 (MQ=255)

           ccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAg                                                            >  2:6594026/1‑76 (MQ=255)

           ccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAg                                                            <  1:6657640/76‑1 (MQ=255)

             gCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGc                                                          <  1:4777855/76‑1 (MQ=255)

             gCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGc                                                          <  1:514875/76‑1 (MQ=255)

             gCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGc                                                          <  2:4211437/76‑1 (MQ=255)

              cTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCa                                                         <  1:585202/76‑1 (MQ=255)

               ttttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCaa                                                        <  1:7133276/76‑1 (MQ=255)

               ttttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCaa                                                        <  2:7675493/76‑1 (MQ=255)

                tttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAAc                                                       >  2:6422532/1‑76 (MQ=255)

                tttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGGCAGGCCGAAAAGGCAAc                                                       >  1:63990/1‑76 (MQ=255)

                 ttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACt                                                      <  2:4587354/76‑1 (MQ=255)

                 ttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACt                                                      <  2:3239169/76‑1 (MQ=255)

                  tACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTg                                                     <  2:2632726/76‑1 (MQ=255)

                  tACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTg                                                     <  1:4300672/76‑1 (MQ=255)

                  tACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTg                                                     >  2:7806286/1‑76 (MQ=255)

                   aCAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTgg                                                    <  2:5969797/76‑1 (MQ=255)

                    cAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGt                                                   <  1:4303597/76‑1 (MQ=255)

                    cAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGt                                                   <  1:688089/76‑1 (MQ=255)

                     aGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTg                                                  <  1:3160398/76‑1 (MQ=255)

                     aGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTg                                                  <  1:1861299/76‑1 (MQ=255)

                     aGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTg                                                  <  1:1219042/76‑1 (MQ=255)

                     aGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTg                                                  <  1:3145858/76‑1 (MQ=255)

                      gCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGa                                                 >  1:6198932/1‑76 (MQ=255)

                      acTACCTGCAATGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGAATGGGTCAGGCCGAAAAGGCAACTGGTGa                                                 <  2:1989678/75‑1 (MQ=255)

                       cTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGAt                                                <  1:3023777/76‑1 (MQ=255)

                        tACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGAtt                                               <  1:6915739/76‑1 (MQ=255)

                         aCCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGAttt                                              >  2:6024230/1‑76 (MQ=255)

                         aCCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGAttt                                              >  2:6024232/1‑76 (MQ=255)

                         aCCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGAttt                                              <  2:7342090/76‑1 (MQ=255)

                          ccTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGAtttt                                             <  1:6491565/76‑1 (MQ=255)

                           cTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGAttttt                                            <  1:3293706/76‑1 (MQ=255)

                               aTCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTattg                                        <  2:4226858/76‑1 (MQ=255)

                               aTCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTattg                                        <  2:4507979/76‑1 (MQ=255)

                                tCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTattga                                       <  2:6052722/76‑1 (MQ=255)

                                 cgcgTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTattgat                                      <  1:3683570/76‑1 (MQ=255)

                                  gcgTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTattgatt                                     <  1:4279773/76‑1 (MQ=255)

                                  gcgTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTattgatt                                     <  1:4116467/76‑1 (MQ=255)

                                   cgTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTattgattg                                    <  2:7424940/76‑1 (MQ=255)

                                   cgTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTattgattg                                    <  1:3289331/76‑1 (MQ=255)

                                    gTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGc                                   <  2:4637326/76‑1 (MQ=255)

                                    ctatGGTCGAAAACCAGCGATAAACCGGTGTGGATGGTTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGc                                   <  2:7237621/73‑1 (MQ=255)

                                      cTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTg                                 >  2:1652381/1‑76 (MQ=255)

                                        ggTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAg                               >  2:2254448/1‑76 (MQ=255)

                                         gTCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGt                              <  2:2844255/76‑1 (MQ=255)

                                          tCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGtt                             <  2:7187990/76‑1 (MQ=255)

                                          tCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGtt                             <  2:651778/76‑1 (MQ=255)

                                          tCGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGtt                             <  1:5146782/76‑1 (MQ=255)

                                           cGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGttt                            >  1:1549204/1‑76 (MQ=255)

                                           cGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGttt                            >  1:2272788/1‑76 (MQ=255)

                                           cGAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGttt                            <  1:4242085/76‑1 (MQ=255)

                                            gAAAACCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGtttt                           <  2:7463615/76‑1 (MQ=255)

                                             aaaaCCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTg                          >  2:1806665/1‑76 (MQ=255)

                                                aCCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAAt                       <  2:3222902/76‑1 (MQ=255)

                                                aCCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAAt                       <  1:5588777/76‑1 (MQ=255)

                                                aCCAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAAt                       <  1:5816268/76‑1 (MQ=255)

                                                 ccAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATa                      <  2:1750895/76‑1 (MQ=255)

                                                 ccAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATa                      <  2:7605159/76‑1 (MQ=255)

                                                 ccAGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATa                      <  1:920165/76‑1 (MQ=255)

                                                   aGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACa                    >  1:2158583/1‑76 (MQ=255)

                                                   aGCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACa                    >  1:7498090/1‑76 (MQ=255)

                                                    gCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACAt                   >  1:849994/1‑76 (MQ=255)

                                                    gCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACAt                   <  2:4059696/76‑1 (MQ=255)

                                                    gCGATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACAt                   >  2:1545241/1‑76 (MQ=255)

                                                      gATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTg                 <  2:7798727/76‑1 (MQ=255)

                                                      gATAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTg                 <  1:7699143/76‑1 (MQ=255)

                                                       aTAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGa                >  2:6621768/1‑76 (MQ=255)

                                                        tAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGaa               <  1:7870255/76‑1 (MQ=255)

                                                        tAAACCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGaa               <  1:2058447/76‑1 (MQ=255)

                                                         aaaCCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGaaa              <  1:2098930/76‑1 (MQ=255)

                                                         aaaCCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGaaa              >  2:2748469/1‑76 (MQ=255)

                                                         aaaCCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGaaa              >  1:2874659/1‑76 (MQ=255)

                                                          aaCCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGaaaa             <  2:220974/76‑1 (MQ=255)

                                                          aaCCGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGaaaa             <  1:594993/76‑1 (MQ=255)

                                                            ccGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAAcc           <  2:2316112/76‑1 (MQ=255)

                                                             cGGTGTGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCg          <  2:7157243/76‑1 (MQ=255)

                                                               gtgtGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGcc        >  2:3838499/1‑76 (MQ=255)

                                                               gtgtGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGcc        <  1:5398017/76‑1 (MQ=255)

                                                               gtgtGGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGcc        <  2:5430317/76‑1 (MQ=255)

                                                                tgtgGATGGGTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCa       <  2:4704298/76‑1 (MQ=255)

                                                                    gatgggtCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAACCCGCCAGCgg   <  2:7522547/76‑1 (MQ=255)

                                                                     atgggtCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCCGCGGa  >  2:6518691/1‑76 (MQ=255)

                                                                     atgggtCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGa  <  2:3369414/76‑1 (MQ=255)

AAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGA  >  REL606/711328‑711472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`