| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,193,779 | 0 | G | T | 99.5% | 725.1 / NA | 191 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (59/131); minor base A (0/1); total (59/132) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 221 total aligned reads displayed.
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851 | gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATcc < 1:1050806/76‑1 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATcccc < 2:4726972/76‑1 (MQ=255) gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCcca < 1:5885352/76‑1 (MQ=255) gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCcca < 2:4116426/76‑1 (MQ=255) gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCGGAAAACAG‑CCCCCATAACAGCAAAACGCCAATCCcca > 1:5553209/1‑76 (MQ=255) aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCccac < 2:7699270/76‑1 (MQ=255) gCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCGGGAAATAG‑GCCCAAAAAAAGCAAAAACCCAAACccccccc > 2:7418669/1‑75 (MQ=255) gCATCCGGCGTCCAGGCGCGGCTGGGCTGGCCCCCCGGAATTTG‑CCCCACTAACAGCAAAAACCCCATCCgcacca > 2:3110075/1‑76 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGTGTTGCTGGCGGGAATCCA‑CCCCCAAAACAACAAAAACCCCAACCccaccca > 2:1863575/1‑74 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAg < 2:5668848/76‑1 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAGCCCCCAAAAAAACCAAAACGCCAACCCccccca > 2:3945294/1‑76 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCGGGAAACAG‑CCCCCATAACAGCAAAAACCCAAcccccccccg > 1:2374929/1‑74 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCGGGAAACAA‑CCCCCATAACAGCAAAAAGCCAATCCccacccc > 1:2931268/1‑74 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGGCGGAAAACAG‑CCCCAATAACAGCAAAACGCCAATCCCCCCCAGa > 1:1576463/1‑76 (MQ=255) tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGAt < 2:3255051/76‑1 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATa < 1:3203162/76‑1 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATa < 2:2130888/76‑1 (MQ=255) cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa < 2:3662774/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAATCAG‑CCCCCATAAAAGCAAAAAACCAAACCCCACCAGAaaaa > 1:382738/1‑76 (MQ=255) ggCGTCCAGGCGCGGCTGGGCTGGCTGTCGGAAAACAA‑CCCCCAATACCGCAAAACACCACACCCCACCAGATaaa > 2:6868827/1‑76 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 1:282526/76‑1 (MQ=255) gCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg < 1:5315360/76‑1 (MQ=255) cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc < 1:4441177/76‑1 (MQ=255) gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa < 1:5210448/76‑1 (MQ=255) ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg < 2:639061/76‑1 (MQ=255) ccAGGCGCGGCTGGGCTGGCTGTCGGAAATCAG‑CCCCCATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg > 1:7081496/1‑76 (MQ=255) cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa < 2:3795982/76‑1 (MQ=255) aGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc < 2:3986787/76‑1 (MQ=255) ggCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg < 2:4168596/76‑1 (MQ=255) ggCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg < 2:7318296/76‑1 (MQ=255) ggCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg < 1:164347/76‑1 (MQ=255) gcgGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 1:3700264/76‑1 (MQ=255) gcgGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 2:643282/76‑1 (MQ=255) ggCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt < 2:1833670/76‑1 (MQ=255) gCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa > 2:4036602/1‑76 (MQ=255) gCTGGTCTGGCTGTCGGAAATCAG‑CCCCAAGAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa < 2:1098551/76‑1 (MQ=255) cTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt < 2:1401730/76‑1 (MQ=255) cTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt < 2:6672653/76‑1 (MQ=255) cTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt > 1:2034979/1‑76 (MQ=255) tGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAGGCATGACGCCTGTAtt > 2:1514178/1‑76 (MQ=255) tGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt > 1:658073/1‑76 (MQ=255) tGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:6420672/76‑1 (MQ=255) tGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:3551861/76‑1 (MQ=255) tGGGCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:1316138/76‑1 (MQ=255) ggTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa < 1:3538213/76‑1 (MQ=255) ggTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa < 2:1271414/76‑1 (MQ=255) gTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 1:2660057/76‑1 (MQ=255) gTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 1:1844211/76‑1 (MQ=255) gTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 2:372116/76‑1 (MQ=255) tCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa > 1:2994738/1‑76 (MQ=255) tCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa > 2:2217779/1‑76 (MQ=255) cTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc > 1:3466063/1‑76 (MQ=255) cTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc > 1:2535494/1‑76 (MQ=255) tGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa < 2:5348044/76‑1 (MQ=255) tGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa > 2:5278763/1‑76 (MQ=255) ggCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg < 1:393305/76‑1 (MQ=255) ggCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg < 2:2296763/76‑1 (MQ=255) gCTGTCGGAAATCAG‑ACCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg < 1:5917990/76‑1 (MQ=255) tGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt < 1:2289149/76‑1 (MQ=255) tGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt > 1:7568925/1‑76 (MQ=255) gTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt < 1:3428586/76‑1 (MQ=255) gTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt < 2:3583930/76‑1 (MQ=255) gTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt < 1:609311/76‑1 (MQ=255) gTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt > 1:5183844/1‑76 (MQ=255) tCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt < 1:2969787/76‑1 (MQ=255) tCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt < 1:7674619/76‑1 (MQ=255) cGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt < 2:2012218/76‑1 (MQ=255) cGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt > 2:4599219/1‑76 (MQ=255) cGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt < 2:3326340/76‑1 (MQ=255) cGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt > 1:3635917/1‑76 (MQ=255) cGGAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt > 1:1464605/1‑76 (MQ=255) ggAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 1:2264473/76‑1 (MQ=255) gAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 2:2080522/76‑1 (MQ=255) gAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa > 2:4806376/1‑76 (MQ=255) gAAATCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa > 2:470473/1‑76 (MQ=255) aaaTCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 1:4148715/1‑76 (MQ=255) aTCAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg < 2:7070479/76‑1 (MQ=255) cAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt < 1:6943810/76‑1 (MQ=255) cAG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt < 1:7686908/76‑1 (MQ=255) aG‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg < 2:1923641/76‑1 (MQ=255) g‑CCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg < 2:1324746/76‑1 (MQ=255) ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc < 2:1718864/76‑1 (MQ=255) ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc < 2:5038996/76‑1 (MQ=255) aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt > 2:4191922/1‑76 (MQ=255) aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc < 1:4350034/76‑1 (MQ=255) aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 1:5229954/1‑76 (MQ=255) aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg < 1:401154/76‑1 (MQ=255) aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg < 2:5022201/76‑1 (MQ=255) aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa < 1:7719215/76‑1 (MQ=255) cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc > 1:7776955/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa > 1:4698347/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:2963330/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 1:4998198/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:4682532/76‑1 (MQ=255) aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc > 1:4901683/1‑76 (MQ=255) aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc < 1:2638925/76‑1 (MQ=255) aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc < 1:3226104/76‑1 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 1:7422654/1‑76 (MQ=255) cAATCCCCACCAGATAAAGCATGCCGCCTGTATTAAACAGGTTTCTTACCGATGGCGCATCGCGCCCCAGCCGCca < 2:6071924/76‑1 (MQ=255) cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca > 1:4548383/1‑76 (MQ=255) | GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAG‑CCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |