| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,131,466 | (A)9→8 | intergenic (‑125/+175) | gatY ← / ← fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,131,458 | 0 | A | . | 97.0% | 486.6 / 8.1 | 101 | intergenic (‑117/+183) | gatY/fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base . (83/15); total (86/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01 | |||||||||||
Only 100 of 150 total aligned reads displayed.
AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATAT > REL606/2131383‑2131532 | aaatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCa < 2:6562722/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa < 1:1073621/76‑1 (MQ=255) aatgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaa > 1:4735436/1‑76 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa > 1:924511/1‑76 (MQ=255) atgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaa < 1:6827530/76‑1 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa < 2:6365538/76‑1 (MQ=255) tgaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaa < 2:5163105/76‑1 (MQ=255) gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa < 2:6191365/76‑1 (MQ=255) gaTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaa > 1:2204931/1‑76 (MQ=255) aTTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaa > 1:1131615/1‑76 (MQ=255) gTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCaaaaaaaacg > 1:5162263/1‑74 (MQ=255) tttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGc > 2:5693954/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 2:5542070/1‑76 (MQ=255) ttAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCt > 1:1917794/1‑76 (MQ=255) tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc > 1:1546701/1‑76 (MQ=255) tAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTc > 1:6080890/1‑76 (MQ=255) aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 2:4798660/1‑76 (MQ=255) aaaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTcc > 1:3894925/1‑76 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTcc > 2:2256803/1‑76 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 1:5601720/1‑76 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 1:5833971/1‑76 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 1:5405850/1‑76 (MQ=255) aaTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTccc > 2:4767151/1‑76 (MQ=255) aTGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCg > 1:3822963/1‑76 (MQ=255) tGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCgg > 2:2212408/1‑76 (MQ=255) tGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCgg > 1:6042801/1‑76 (MQ=255) tGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCgg > 2:258103/1‑76 (MQ=255) gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGCCCCCCCgg > 1:513260/1‑76 (MQ=255) gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGa > 1:1306899/1‑76 (MQ=255) gTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGa > 1:5447629/1‑76 (MQ=255) tCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAg > 2:4177423/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 1:3424543/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg > 2:1915317/1‑76 (MQ=255) cGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg < 1:6088927/76‑1 (MQ=255) gagaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg‑g < 2:4603310/76‑1 (MQ=255) gagaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg‑g < 1:6398332/76‑1 (MQ=255) gagaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAgg‑g < 2:2074416/76‑1 (MQ=255) gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑aa < 2:3507402/76‑1 (MQ=255) gaTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑aa < 2:5114010/76‑1 (MQ=255) aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAg > 1:568141/1‑76 (MQ=255) aTAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAg > 1:4854822/1‑76 (MQ=255) tAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGc > 1:6033556/1‑76 (MQ=255) aaCGATCACAAAAACGACAATGTGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGcc > 2:2851652/1‑76 (MQ=255) aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAgac > 2:3805935/1‑74 (MQ=255) aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGcc < 1:4632175/76‑1 (MQ=255) aaCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGcc > 2:3940403/1‑76 (MQ=255) aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCg < 1:1459410/76‑1 (MQ=255) aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCg < 2:1299446/76‑1 (MQ=255) aCGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGACg > 2:4457767/1‑76 (MQ=255) cGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGc > 1:5100416/1‑76 (MQ=255) aTCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCCCCCGGAGG‑G‑AAGCCGcaa < 1:6613248/76‑1 (MQ=255) cacaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGcaaca > 1:1267655/1‑76 (MQ=255) cacaAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGcaaca > 1:5169302/1‑76 (MQ=255) aaaaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAg > 2:5211567/1‑76 (MQ=255) aaaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGt > 1:6360542/1‑76 (MQ=255) aaaaCGAAAATATAAAAATTAATAGA‑GAAAGAAAAGAAAAAAAAACCGCCCCCCGGGAGG‑A‑AAGCCGCAACAAAGt < 2:4301451/76‑1 (MQ=255) aaaCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTg > 1:1973219/1‑76 (MQ=255) aacaacAAAATGAAAATAAAAAGAGAAATAAAAGG‑AAAAAAAACCGCCCCCCCGGAGG‑A‑AAGCCGCAACAAAGTgc < 2:1993629/76‑1 (MQ=255) aCGACAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTgcg > 1:79823/1‑76 (MQ=255) caaCAAAATAAAAATAAATAGAGGAATAAAAGG‑AAAAAAAAACGGCCCCCCGGGGG‑G‑AAGCCGCAACAAAGTgcgc < 1:2908681/74‑1 (MQ=255) aCAATATGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGt > 2:3850895/1‑76 (MQ=255) atatGAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGt > 1:2969446/1‑76 (MQ=255) agaaaaaTAAATAGAGAAATGAAAGG‑AAAAAAAAACGGCCCCCCGGAGG‑A‑AAGCCGCAACAAAGTGCGCGTGGttt < 2:4498495/73‑1 (MQ=255) tGAAAATTAATAGAGGAATGAAAGGC‑AAAAAGAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGtttt > 2:5396494/1‑76 (MQ=255) agAAAATAAAGAGA‑GAAAAAAAAGAAAAAAAAACCGCCCCCCGGGAGG‑A‑AAGCCGCAACAAAGTGCGCGTGGtttt < 2:275457/75‑1 (MQ=255) taaaaTTAAGAGAGAAATGAAAGGC‑AAAAAAACCGGCCCCCGGGAGG‑A‑AAGCCGCAACAAAGTGCGCGTGGTTTTa < 1:5606572/75‑1 (MQ=255) gAAAATTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTa > 2:6740287/1‑76 (MQ=255) aaaaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTAt > 2:3609491/1‑76 (MQ=255) aaaaTAAAAAGAGGAAGGAAAG‑‑AAAAAAAAACCGCCCCCCGGGGG‑GAAAGCCGCAACAAAGTGCGCGTGGTTTTAt < 2:5586364/76‑1 (MQ=255) aaataaatAGAGGAATGAAAGG‑AAAAAAAAACGCCCCCCGGGAGG‑A‑AAGCCGCAACAAAGTGCGCGTGGTTTTATg < 2:1306899/76‑1 (MQ=255) aaaTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCCCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATg < 1:2522079/76‑1 (MQ=255) aaaTTAATAGAGGAATGAAA‑GCAAAAAAAAACGGCCCCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATg < 1:3439237/76‑1 (MQ=255) aaaTAAAGAGAGGAAAAAAAGG‑AAAAAAAAACGCCCCCCGGGAGG‑A‑AAGCCGCAACAAAGTGCGCGTGGTTTTATg < 2:2967603/76‑1 (MQ=255) aaTTATTAGAGTAATGAAAGGC‑AAAAAATTCGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGc < 2:1552265/76‑1 (MQ=255) aTTAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCa > 1:661192/1‑76 (MQ=255) aTTAATAGAGGAATGAAAGG‑AAAAAAAACCGCCCCCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCa < 1:402236/76‑1 (MQ=255) aTTAATAGAGAAATAAAAGGC‑AAAAAAAACGGCCCCCCGGAGG‑A‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCa < 1:3929842/76‑1 (MQ=255) tAATAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAgg > 1:4181974/1‑76 (MQ=255) aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa > 2:770842/1‑76 (MQ=255) aaTAGAGGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGa > 1:494462/1‑76 (MQ=255) agagagGAAAGAAA‑GAAAAAAAAACCGCCCCCCCGGAGG‑A‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt < 2:6779705/74‑1 (MQ=255) aTAGAGAAATGAAAGG‑AAAAAAAAACGGCCCCCCGGAGG‑A‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGAt < 1:930868/76‑1 (MQ=255) agagGAATGAAAGGC‑AAAAAAAACGGCCTTCCGGAGG‑G‑AAGCCGAAACAAAGTGCGCGTGGATTTATGCAGGATgg > 1:4015768/1‑75 (MQ=255) agagAAAGGAAA‑GCAAAAAAAAGCGGCCCCCGGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc < 2:4421070/76‑1 (MQ=255) gagGAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcg > 2:4127184/1‑76 (MQ=255) aaagaaagaaaAGAAAAAAAAACCGGCCTCCGGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgc < 1:2260603/73‑1 (MQ=255) gAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggcg > 2:5983191/1‑76 (MQ=255) gAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggcg > 2:793845/1‑76 (MQ=255) gAATGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATgcggcg > 2:4020464/1‑76 (MQ=255) aaTGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGt > 2:6171142/1‑76 (MQ=255) aTGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa > 1:49947/1‑76 (MQ=255) aTGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGAGCGCGTGGTTTTATGCAGGATGCGGCGTa > 1:6751282/1‑76 (MQ=255) tGAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa > 2:2576681/1‑76 (MQ=255) agAAAGG‑AAAAAAAAACGGCCTCCCGGAGG‑A‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaa < 1:2539824/75‑1 (MQ=38) agAAAAGCAAAAAAAACCGGCCTCGCGGAGA‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTa < 2:4255712/75‑1 (MQ=38) gAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTaaa > 2:3154179/1‑76 (MQ=255) gAAAGGC‑AAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGGGCGCGTGGTTTTATGCAGGATGCGGCGTaaa > 1:739260/1‑76 (MQ=39) aggaaaaaaaacCGCCCCCCCGGAGG‑A‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACat < 1:3748556/73‑1 (MQ=37) ggaaaaaaaagCGGCCCCCGGGAGG‑A‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACata < 2:5927114/74‑1 (MQ=37) aaaaaaataCGTCCTCCCGGGGGAA‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACatat < 2:4553216/76‑1 (MQ=25) | AAATGATTTGTTTTTAAATGTCGAGATAACGATCACAAAAACGACAATATGAAAATTAATAGAGGAATGAAAGGCAAAAAAAAACGGCCTCCCGGAGG‑G‑AAGCCGCAACAAAGTGCGCGTGGTTTTATGCAGGATGCGGCGTAAACATAT > REL606/2131383‑2131532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |