| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,376,348 | 0 | T | G | 43.7% | 211.2 / 42.9 | 151 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
| Reads supporting (aligned to +/- strand): ref base T (21/64); new base G (66/0); total (87/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.43e-24 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 203 total aligned reads displayed.
GCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376273‑2376423 | cACCAGATGAGGCGATCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtg < 1:6039424/76‑1 (MQ=255) cccAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtgg < 1:2603165/75‑1 (MQ=255) aCCAGATGAGGCAACCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtgg < 2:410307/76‑1 (MQ=255) cAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGACTCGACTCCAGCGTACGGCAggtgggg > 1:1583276/1‑74 (MQ=255) aGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGggggtgg > 2:2064951/1‑75 (MQ=255) tGAGGCGAACTGTAGACTCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCtt < 1:1527410/76‑1 (MQ=255) tGAGGCGAACTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCtt < 2:1940732/76‑1 (MQ=255) gAGGCGAACTGTAGCCGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCttt < 1:3324930/76‑1 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCttt < 2:1244130/76‑1 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCttt < 1:3986220/76‑1 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCttt < 1:2945941/76‑1 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCtct > 1:5544769/1‑74 (MQ=255) aGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGGGCCCTTg > 2:2601839/1‑76 (MQ=255) aGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCtttt > 1:908927/1‑75 (MQ=255) gCGACCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGcc < 2:2086456/76‑1 (MQ=255) gCGAACTGGAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGGTTTGcc > 1:3222967/1‑76 (MQ=255) cGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGGGCCTTTGCCa > 2:6789731/1‑76 (MQ=255) cGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCCTTggcc > 1:2317594/1‑73 (MQ=255) gAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCAt > 2:1068046/1‑76 (MQ=255) gAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCCTTGGCAt > 2:1827355/1‑76 (MQ=255) aaCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCact > 1:6495515/1‑74 (MQ=255) aaCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGCCAtt > 1:2010686/1‑76 (MQ=255) cTGTAGACGCCCTCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCg < 1:5913589/76‑1 (MQ=255) cTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCg < 2:5308748/76‑1 (MQ=255) cTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGGGGCTTTGCCATTCg > 1:4303679/1‑76 (MQ=255) tGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGt > 1:2782857/1‑76 (MQ=255) gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCCTTGCCCTTTGTa > 1:5814623/1‑76 (MQ=255) aGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCCTTGCCCTTCGTAcc > 1:415066/1‑76 (MQ=255) aGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGCCATTCGTAcc > 1:2148575/1‑76 (MQ=255) gccGCCCACTTGTTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAccc < 1:6288151/74‑1 (MQ=255) gACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGGGTACGGCAGGTGGTGCCTTTGCCATTCGTAccc < 1:850739/76‑1 (MQ=255) gCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGCCATTCGTAACCGCa > 1:3928256/1‑76 (MQ=255) cgttttGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACg < 1:1481680/73‑1 (MQ=255) cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCCTTGCCATTCGTAACCCCaaag > 2:5581788/1‑74 (MQ=255) cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGGCTTTGCCATTCGTACCCCCAACg > 2:2837682/1‑76 (MQ=255) cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGGCATTCGTACCCGCAACg > 2:4840382/1‑76 (MQ=255) aCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACgg < 1:5746451/76‑1 (MQ=255) aCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACgg > 1:1947449/1‑76 (MQ=255) aCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACgg > 2:1783266/1‑76 (MQ=255) ttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTTGCATTTGTACCCGCAACGGTaa > 2:5804306/1‑76 (MQ=255) ttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGGaa > 1:511949/1‑76 (MQ=255) tgcccTGCCGCCTTCAGAATCGACTCCAGCGTTCGTCAGGTGGTGCCTTTTCCATTCGTACCCGCAACGGTAAaca < 2:1646643/74‑1 (MQ=255) tcccccGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAaca < 2:3910286/74‑1 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGGGCCTTTTCCATTCGTACCCGCAACCGTAAaca > 2:5651523/1‑76 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCCACGGGAAaca > 2:6516848/1‑76 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCCACGGGAAacac > 2:1145520/1‑76 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAAACGGAAacac > 2:4329550/1‑76 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGGAAacac > 2:3039769/1‑76 (MQ=255) cccTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAAaaca > 2:3238313/1‑76 (MQ=255) cggcccccATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaaa < 1:4428763/74‑1 (MQ=255) cTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaaa < 1:497700/76‑1 (MQ=255) tGCCGCCATCAGAATCGACTCCAGCGTACGGCCGGGGGGGCCCTTTTCCTTCGTAACCGCAACGGCAAACACAAAt > 1:1827761/1‑76 (MQ=255) tGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt > 1:2409723/1‑76 (MQ=255) gccgccATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTTCCAATCGTACCCGCAACGGTAAACACCAAtc > 1:6901458/1‑75 (MQ=255) cccgccATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATg < 1:2074349/75‑1 (MQ=255) ccgccATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGGCATTCGTACCCGCAACGGGAAACACAAAAgg > 1:1378198/1‑76 (MQ=255) cgccATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAAGgc > 2:6657299/1‑76 (MQ=255) gccATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACCAATGgcg > 2:4416918/1‑76 (MQ=255) ccATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTTCCATTCGTACCCGCAACGGTAAACACAAATGgcgc > 2:1501836/1‑76 (MQ=255) ccATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAAAgcgc > 2:5518808/1‑76 (MQ=255) cATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCt > 1:1079674/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCAGGTGGGGCCTTTGCCATTCGTACCCGCAACGGTAAAAACAACAGGGGCTg > 2:3468552/1‑76 (MQ=255) tCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAAGGCGCTgg > 2:2895147/1‑76 (MQ=255) tCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTgg > 1:1801570/1‑76 (MQ=255) cAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTTCACTTCGTACCCGCAACGGTAAACACCAACGGCGCTggg > 2:3360971/1‑75 (MQ=255) cAGAATCGACTCCAGCGTACGGCAGGGGGGGCCCTTGCCATTCGTACCCGCAACGGAGAACACAAATGGCGCTggg > 2:6299520/1‑75 (MQ=255) aGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGtt < 2:5744562/76‑1 (MQ=255) aGAATCGACTCCAGCGTACGGCAGGTGGGGCCTTTGCCATTCGTACCCGCCACCGTAAACACAAATGGCGCCgggt > 1:4537657/1‑74 (MQ=255) aGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGACATTTTTACCCCCAACGGTAAACACCAATTGGGCTgggt > 1:359165/1‑74 (MQ=255) aGAATCGACTCCAGCGTACGGCAGGGGGGGCCCTTTTCCTTCGTACCCGCAACGGTAAACACAAAAGGCGCTGGtt > 1:5451691/1‑76 (MQ=255) gAATCGCCTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGttt < 2:2266951/76‑1 (MQ=255) tCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAg < 2:5143729/76‑1 (MQ=255) cgccTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 2:775316/73‑1 (MQ=255) cGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 2:519784/76‑1 (MQ=255) cGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 1:432210/76‑1 (MQ=255) cGACCCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 2:3724806/76‑1 (MQ=255) gccTCGAGCATACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGa < 2:6239000/74‑1 (MQ=255) gACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGa < 2:214075/76‑1 (MQ=255) cTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACg < 2:4277983/76‑1 (MQ=255) cTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACg < 2:6015025/76‑1 (MQ=255) ccAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGcc < 1:2698017/76‑1 (MQ=255) aGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCaa < 2:2770503/76‑1 (MQ=255) gCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 2:2742429/76‑1 (MQ=255) gCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 2:4578231/76‑1 (MQ=255) gCGTACGGCAGGGGGTGCCTTTGCCATTAATACCCGCAACGGTAAACACAAATGGCGCTGGGTTCAGGACGGCAAg > 2:6740586/1‑76 (MQ=255) cGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGa < 1:2488348/76‑1 (MQ=255) gtccggcggGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAc < 2:2969085/73‑1 (MQ=255) gTAGGGCGGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAc < 1:1213405/76‑1 (MQ=255) gTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAc > 1:172570/1‑76 (MQ=255) tACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcg < 1:4623210/76‑1 (MQ=255) tACGGCAGGGGGGGGCTTTTCCATTCGTTCCCGCAACCGGAAACACAAACAGCGCCGGGTTTAGGACGCCAAGAcc > 2:4246791/1‑75 (MQ=255) aCGGCAGGGGGGGCCTTTGCCATTCGTAACCGCAACGGGAAACACAAAAGGGGCTGGTTTTAGGGCGCCCAGAccc > 2:1958423/1‑74 (MQ=255) cAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccg > 1:6161269/1‑76 (MQ=255) cAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccg < 1:710906/76‑1 (MQ=255) cAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccg > 1:2747651/1‑76 (MQ=255) cAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccg > 2:4002180/1‑76 (MQ=255) aGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccgc > 1:3633750/1‑76 (MQ=255) aGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGcgccgc > 1:884087/1‑76 (MQ=255) ggtggtGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCcgcg < 2:3623656/76‑1 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 1:5263593/1‑76 (MQ=255) | GCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376273‑2376423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |