| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,061,648 | 0 | A | C | 35.2% | 342.4 / 35.6 | 159 | G40G (GGT→GGG) | yccY | phosphotyrosine‑protein phosphatase |
| Reads supporting (aligned to +/- strand): ref base A (85/18); new base C (0/56); total (85/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.74e-27 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 202 total aligned reads displayed.
TCAAGAGATACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATGGGCGAACGGCAAATG > REL606/1061574‑1061720 | tCAAGAGATACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTTCCAGACCATGAacac > 1:743545/1‑76 (MQ=255)tCAAGAGATACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGGGTTTTACCAGACCATGAacac > 1:6764359/1‑76 (MQ=255) aaGAGATACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGTGTGTTTTACCAGACCATGAACACCt < 2:3264210/76‑1 (MQ=255) aaGAGATACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCt > 2:5125034/1‑76 (MQ=255) aaGAGATACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCt < 2:3169364/76‑1 (MQ=255) aaGAGATACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGGGTTTTACCAGACCCTGAAAACCt > 2:1523336/1‑76 (MQ=255) agagATACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTg < 2:901195/76‑1 (MQ=255) agagATACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTg < 1:4163269/76‑1 (MQ=255) agagATACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTg < 1:1892424/76‑1 (MQ=255) agaTACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGGGTTTTACCAGACCATGAACACCTGcc > 1:2241162/1‑76 (MQ=255) gaTCCGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCg < 1:6741995/76‑1 (MQ=255) gaTACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCg < 2:1155279/76‑1 (MQ=255) gaTACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCg < 1:6147520/76‑1 (MQ=255) aTACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGGGGTTTACCAGACCATGAACACCTGCCGa > 2:302434/1‑76 (MQ=255) tACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGAt > 2:6281658/1‑76 (MQ=255) tACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGGGTTTTACCAGACCATGAACACCTGCCGAt > 1:1415868/1‑76 (MQ=255) aCGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGAtt < 2:3321095/76‑1 (MQ=255) aCGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGAtt < 2:1956514/76‑1 (MQ=255) cGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGAttt < 2:4652006/76‑1 (MQ=255) cGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGAttt > 1:911388/1‑76 (MQ=255) ccATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGa < 2:5841613/76‑1 (MQ=255) gATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACttt > 1:2936530/1‑76 (MQ=255) tttAGCGGCACCAGCTGCTGCTGTCGCATCTGCAGGGTGTTTTCCCAGACCATGAACCCCTGCCGATTTGACtttt < 2:5181452/75‑1 (MQ=255) aTTGGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACtttt < 1:6347650/76‑1 (MQ=255) aTTAGCGGCACCATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACtttt < 1:3161454/76‑1 (MQ=255) aTTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACtttt > 1:2630754/1‑76 (MQ=255) aTTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACtttt > 1:2045801/1‑76 (MQ=255) gccGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACg < 1:4927137/74‑1 (MQ=255) gcgcCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGc < 1:6433960/76‑1 (MQ=255) gCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGc < 1:6695234/76‑1 (MQ=255) cGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGcc > 2:2791969/1‑76 (MQ=255) cGGCAACATCTGCTGCTGTCGCATCGGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGcc < 1:1580775/76‑1 (MQ=255) gaaaCCTCTGCTGCTGTCGCATCTGCAGGGTGTTTTCCCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTg < 2:4188369/74‑1 (MQ=255) cAACATCTGCTGCTGGCGCATCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTgg < 2:1927518/76‑1 (MQ=255) aaCATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGc > 2:838187/1‑76 (MQ=255) aCATGTTCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCa < 2:6900272/76‑1 (MQ=255) aCATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCa > 2:4810958/1‑76 (MQ=255) aCATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCa > 2:5534204/1‑76 (MQ=255) aCATCTGCTGCGTTCGCATCTGCAGGGTTTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCa < 1:1678710/76‑1 (MQ=255) cttCTGCTGCTGTCGCATCTGCAGGGTTTTTTCCCAGACCATAACCCCCTGCCGATTTGACTTTTACGCCTGGCAg < 2:271220/74‑1 (MQ=255) cttCTGCTGCTGTCGCATCTGCAGGGTTTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAg < 2:1010518/74‑1 (MQ=255) cATCTGCTGCTGTCGCATCGGCAGGGTGTTTTCCCAGACCATGACCCCCTGCCGATTTGACTTTTACGCCTGGCAg < 2:3486965/76‑1 (MQ=255) cATCTGCGCCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAg < 1:1097845/76‑1 (MQ=255) aaTCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGCCCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAg < 1:3111596/75‑1 (MQ=255) aTCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGa > 2:1387388/1‑76 (MQ=255) ctgctgctgTCGCATCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCt < 1:1500619/68‑1 (MQ=255) tgctgctgTCGCATCTGCAGGGTTTTTTACCAGCCCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGt < 2:5904315/76‑1 (MQ=255) ttgctgTCGCATCTGCGGGGTTTTTTCCCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGttt < 2:2313634/75‑1 (MQ=255) ctgctgTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGttt > 1:1034864/1‑76 (MQ=255) ctgctgTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGttt > 1:4305508/1‑76 (MQ=255) tgctgTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTa < 2:3381174/76‑1 (MQ=255) gctgTCGCATCTGCAGGGTGTTTTACCAGACCATGACCCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTAc < 2:3846375/76‑1 (MQ=255) gctgCCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTag > 2:1459900/1‑75 (MQ=255) ctgTCGCATCTGCAGGTTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACg < 2:6219816/76‑1 (MQ=255) ctgTCGCATCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACg < 1:3419399/76‑1 (MQ=255) ctgTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACg > 1:6633456/1‑76 (MQ=255) gTCGCTTCTGCAGGGTTTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACgca < 2:1809526/76‑1 (MQ=255) gTCGCATCTGCAGGGTGTTTTCCCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACgca < 1:5142041/76‑1 (MQ=255) gTCGCATCTGCAGGGTGTTTTCCCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACgca < 1:6833573/76‑1 (MQ=255) gTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACgca > 1:5688751/1‑76 (MQ=255) tCGCATCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACgcag < 2:3677126/76‑1 (MQ=255) cGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACgcagc > 2:1329383/1‑76 (MQ=255) cGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACgcagc > 1:6236430/1‑76 (MQ=255) gCATCTGCGGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACgcagca < 1:3671904/76‑1 (MQ=255) gCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACgcagca > 2:751214/1‑76 (MQ=255) gCATCGGCGGGGGTTTTTACCAGACCATGAACTCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACgcagca < 2:6687088/76‑1 (MQ=255) cATCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCaa < 2:1573969/76‑1 (MQ=255) cATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCaa > 1:1803760/1‑76 (MQ=255) cATCGGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCaa < 1:5215551/76‑1 (MQ=255) aTCTGCGGGGTGTTTTCCCAGCCCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAg < 1:3045341/76‑1 (MQ=255) aTCTGCAGGTTGTTTTACCAGACCATGACCCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAg < 2:3238046/76‑1 (MQ=255) aTCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAg > 1:4938434/1‑76 (MQ=255) aTCCGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAg > 1:440187/1‑76 (MQ=255) ttggCAGGGTGTTTTCCGACCCCAAGACCCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAgc < 1:3957404/73‑1 (MQ=255) tCTGCAGGGTTTTTTCCCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAgc < 2:2449808/76‑1 (MQ=255) tCTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAgc < 1:4735356/76‑1 (MQ=255) cTGCAGGGTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAgcg < 1:2287805/76‑1 (MQ=255) tGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAgcgc > 2:6076646/1‑76 (MQ=255) ggggggTGTTTTACCAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCt < 2:2227599/73‑1 (MQ=255) aGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCg > 1:2810145/1‑76 (MQ=255) aGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCg > 1:5264921/1‑76 (MQ=255) aGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCg > 2:215388/1‑76 (MQ=255) gggTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGc > 1:4138505/1‑76 (MQ=255) gtgtTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCa > 1:5519727/1‑76 (MQ=255) gtTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAAt > 1:194756/1‑76 (MQ=255) ttttACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATg > 2:2640492/1‑76 (MQ=255) tttcACCAGCCCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATg < 2:17226/76‑1 (MQ=255) tttACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATgg > 1:1370528/1‑76 (MQ=255) cccAGACCATGAACCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATGGGCg < 2:5956761/75‑1 (MQ=255) ccAGCCCATAACCCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATGGGCGa < 1:244670/76‑1 (MQ=255) ccAGACCATGACCCCCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATGGGCGa < 2:676817/76‑1 (MQ=255) ccAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATGGGCGa > 1:5824323/1‑76 (MQ=255) aGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATGGGCGAAc > 1:304146/1‑76 (MQ=255) gACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATGGGCGAACg > 1:127485/1‑76 (MQ=255) ccATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATGGGCGAACGGc < 1:3398887/76‑1 (MQ=255) cATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATGGGCGACCGGCa > 1:5650258/1‑76 (MQ=255) aTGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATGGGCGAACGGCaa > 2:5650466/1‑76 (MQ=255) tGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATGGGCGAACGGCaaa > 2:3869753/1‑76 (MQ=255) tGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATGGGCGAACGGCaaa > 2:6408199/1‑76 (MQ=255) aaCACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATGGGCGAACGGCAAATg > 1:4535455/1‑76 (MQ=255) | TCAAGAGATACGCCATGATTAGCGGCAACATCTGCTGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCTGGCAGACGTTTACGCAGCAAGCGCTCGCCAATGGGCGAACGGCAAATG > REL606/1061574‑1061720 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |