| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,118,209 | 0 | G | T | 36.2% | 55.6 / 40.1 | 47 | intergenic (‑444/‑311) | csgD/csgB | DNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex |
| Reads supporting (aligned to +/- strand): ref base G (7/23); new base T (17/0); total (24/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.63e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 104 total aligned reads displayed.
AAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACG > REL606/1118136‑1118284 | aaaCGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTtgtg < 2:6611089/76‑1 (MQ=255) aaCGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTgc < 1:2788225/76‑1 (MQ=255) cTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGTGCTAGa > 1:525266/1‑76 (MQ=255) cTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGa < 1:6649403/76‑1 (MQ=255) cTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGa < 2:2383924/76‑1 (MQ=255) tGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTTGCAAGACa > 1:5025975/1‑76 (MQ=255) tGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTCGCCAGACa > 2:4125253/1‑76 (MQ=255) tGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGCGCAAGGCa > 1:6887840/1‑76 (MQ=255) tGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACa < 2:6732053/76‑1 (MQ=255) tGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACa < 1:963349/76‑1 (MQ=255) tGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACa < 2:2501508/76‑1 (MQ=255) ttttttCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACata < 1:1510262/76‑1 (MQ=255) ttttttCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACata < 1:4169471/76‑1 (MQ=255) ttttttCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACata < 1:3707151/76‑1 (MQ=255) tttttCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTTGCCAGACatat > 1:6167960/1‑76 (MQ=255) tttttCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGTGCCAGAAatat > 1:5394601/1‑76 (MQ=255) ttttCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTTGCACAACATATc > 1:6129532/1‑76 (MQ=255) ttCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTCGCCAGACATATCgc > 1:676180/1‑76 (MQ=255) ttCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGTGCCAGACATATCgc > 1:3508943/1‑76 (MQ=255) ttCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGCGCAAGACAAATCgc > 2:6634383/1‑76 (MQ=255) tctcCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTGGCCAGACAAAACgca > 2:3773734/1‑76 (MQ=255) ctcCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTGGCCAGACATATCgtcg > 1:298946/1‑73 (MQ=255) ctcCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGTGCACGACCTATTgcac > 1:704091/1‑75 (MQ=255) tcCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTTGCAAGACATATCgcagc > 1:2234580/1‑76 (MQ=255) ccACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGTGGAAGACAAATCgcagca > 1:1411797/1‑76 (MQ=255) ccACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGTGCCAGACCTATCgcagca > 1:142807/1‑76 (MQ=255) ccACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGGGCCAGAAATATCtcagca > 2:4123744/1‑76 (MQ=255) accccTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAAt < 1:5793208/73‑1 (MQ=255) acacCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTCGCAAGACATATCGCAGCAAt > 2:5990207/1‑76 (MQ=255) acacCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGTGCCAGACATATCGCAGCaaa > 2:4515528/1‑75 (MQ=255) acacCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGGGCGCAACATATCgcagcgaa > 2:463756/1‑73 (MQ=255) ccccTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATc < 1:3287327/74‑1 (MQ=255) ccccTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATc < 1:1916200/74‑1 (MQ=255) ccccTCCGTGGAAATTTTTTTACTGCAAAAAGACGAGTTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATc < 1:2894071/74‑1 (MQ=255) ccTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCCCGGCTTGTGCGAAAGACATATCGCAGCAATCAg < 2:4387446/76‑1 (MQ=255) ccTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTCGCCAGACCAATTCCAGCAAACaa > 1:839052/1‑75 (MQ=255) cTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGTGCAAGACAAATCGCAGCAATCaac > 2:5407231/1‑74 (MQ=255) cTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGGGGAAGACATATTGCAGCAATCAGc > 1:2970202/1‑76 (MQ=255) cTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGCGCCAGACATATCGCAGCAATCCGc > 2:3279263/1‑76 (MQ=255) ccGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGCGCAAGAAATATCGCAGCCATCAGCgc > 2:3560592/1‑75 (MQ=255) cGTGGACTATTTTTTACTGAAAAAAGCGGAGGTTTGTCCGGTCTTGTGCAAAAGACATATCGCAGCAATCAGCGAc < 2:2692423/76‑1 (MQ=255) gTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGGGCCAGAAAAATTGCAGCAAACAGCGCCg > 1:1435748/1‑76 (MQ=255) tGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGTGCAAGACATATAGCAGCAATCAACGAccg > 2:1865278/1‑74 (MQ=255) ggACAATTTTTTACTGAAAAAAGACGAGGTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACggg < 1:3982923/76‑1 (MQ=255) gccAATTTTTTACGGCAAAAAGACGAGTTTTGTCGCGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGc < 1:4200328/74‑1 (MQ=255) cAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGCGCAAGACCAATCGCAGCAATCAGCGACggggca > 2:5450695/1‑73 (MQ=255) atttttttACTGCAAAAAGACGAGGTTTGTCCCGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCaag < 2:489027/74‑1 (MQ=255) aTTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTCGCAAGACAAATCGCAGCAATCAGCGACGGGCgaga > 2:1831686/1‑76 (MQ=255) aTTTTTTACTGAAAAAAGACGAGTTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCaaga < 1:4403513/76‑1 (MQ=255) ttttttACTGCAAAAAGACGAGTTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCaagaa < 1:1573059/76‑1 (MQ=255) ttttttACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCaagaa < 2:5989401/76‑1 (MQ=255) ttttttACTGAAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCaagaa < 2:5212182/76‑1 (MQ=255) ttttttACGGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCaagaa < 2:114280/76‑1 (MQ=255) tttttACTGAAAAAAGACGAGTTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCaagaag < 1:1884276/76‑1 (MQ=255) ttttACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGTGGAAGACATATCGCAGCAATCAGAGACCGGGCagaaga > 1:1815216/1‑76 (MQ=255) tttACTGCAAAAAGACGAGGTTTGTCACGGCTTTTGGGCCAGACATATTCCAGCAAACAACGACGGGGCAaaagaa > 2:3974039/1‑76 (MQ=255) tttACTGCAAAAAGACGAGGTTTGTCACGGCTTTCGCGGGAGACACAACTAAGACATCAACGACCGGGCAgaagaa > 2:6649489/1‑76 (MQ=255) tttACTGAAAAAAGACGAGTTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAgaagaa < 1:6482282/76‑1 (MQ=255) ttACTGCAAAAAGACGGGGTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAAt < 1:2964048/76‑1 (MQ=255) tACTGCAAAAAGACGAGGTTTGTCACGGCTTTTTGGCCAGGAATATCGCAGGAAATAGCCACGGGGCAgaagaaag > 1:3744854/1‑74 (MQ=255) tACTGANAAAAGACGAGTTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATg < 2:53/76‑1 (MQ=255) tACTGAAAAAAGACGAGGTTTGTCCCGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATg < 1:6348895/76‑1 (MQ=255) aCTGCAAAAAGAGGAGGTTTGTCACGGCTGGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGa < 1:6794487/76‑1 (MQ=255) aCTGCAAAAAGACGAGGTTTGTCACGGCTTTTGGGCACGACATATAGCAGCAAACAGCGACGGGCAAGaagaaaga > 2:4179029/1‑76 (MQ=255) aCTGAAAAAAGACGAGGTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGa < 2:1983555/76‑1 (MQ=255) tGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACt < 2:2296079/76‑1 (MQ=255) cAAAAAGACGAGGTTTGTCACGGCTTTTGTGCAAAACAAAACCCAGCAATAACCGACGCGCCAGAAGAAAGACTGt > 2:1459671/1‑76 (MQ=255) cAAAAAGACGAGGTTTGTCACGGCTTTTGGGCCAGAAATATCGCAGCAAACAGCGACGGGGAAAAAGAAAGACTGt > 2:5888607/1‑76 (MQ=255) aaaaaGACGAGGTTTGTCACGGCTTTTGGGCAAGACATAACGCCGCCAACAACGAAGGGGCAGAAAAAAGAATGTc > 2:112062/1‑76 (MQ=255) aaaGGCGAGGTTTGTCACGGTTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTg < 2:3164080/76‑1 (MQ=255) aaaGACGAGGTTTGTCCCGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTg < 2:1824628/76‑1 (MQ=255) aaaGACGAGGTTTGTCACGGCTTTTGCGCAAGACAAATCGCCGCAAAAAGCGACGGGGCAGAAGAAAGACTGTCTg > 1:3174517/1‑76 (MQ=255) aaGACGGGGTTTGCCCCGGTTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTgg < 2:1904515/76‑1 (MQ=255) aaGACGAGGTTTGTCGCGGTTTGTGCAAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTgg < 1:227171/76‑1 (MQ=255) aaGACGAGGTTTGTCACGGCTTTTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTgg > 1:2636891/1‑76 (MQ=255) aCGAGGTTTGTCACGGCTTTTTCGCAAGACATATATCCGCAATCAGCGACGGGGAAGAAGAATGACTGTCTtgtgg > 2:1370009/1‑75 (MQ=255) gggTTTGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCttt < 1:4941509/75‑1 (MQ=255) gttttGTCACGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCtttt < 2:1382377/74‑1 (MQ=255) ggTTTTTCACGTCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCtttt < 1:1634894/76‑1 (MQ=255) ggTTGGTCACGGCTTGGGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCtttt < 1:2385539/76‑1 (MQ=255) gTTTGTCAGGGCTTGTGCAAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCttttt < 1:5722083/76‑1 (MQ=255) tttGTCACGGTTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTg < 1:480351/76‑1 (MQ=255) tCACGTCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAg < 2:2796584/76‑1 (MQ=255) aCGGCTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCg < 1:5006148/76‑1 (MQ=255) cGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCgg < 2:4719498/76‑1 (MQ=255) cTTGTGCGCAAGATATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGaaa < 1:3317717/76‑1 (MQ=255) cTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGaaa > 1:6563977/1‑76 (MQ=255) cTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGaaa > 1:4682175/1‑76 (MQ=255) cTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGGGCTTTTTGATAGCGGaaa > 1:1271272/1‑76 (MQ=255) cTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGCGCTTTTCGATAGAGGtaa > 2:3938636/1‑76 (MQ=255) cTTGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGaaa < 2:3531129/76‑1 (MQ=255) ttGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGaaaa < 2:487659/76‑1 (MQ=255) ttGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGaaaa > 2:5550088/1‑76 (MQ=255) ttGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGaaaa > 2:407088/1‑76 (MQ=255) ttGTGCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGaaaa < 2:3468772/76‑1 (MQ=255) ttGTGCAAAAGACATATCGCAGCAATCAGCGACGGACAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGaaaa < 1:6484144/76‑1 (MQ=255) tgtgCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAAc < 1:6957661/76‑1 (MQ=255) ggtgCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAAc < 2:1459603/75‑1 (MQ=255) gtgCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACg > 1:2551094/1‑76 (MQ=255) gtgCGAAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACg < 1:3154807/76‑1 (MQ=255) | AAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACG > REL606/1118136‑1118284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |