| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,485,450 | 0 | A | G | 36.0% | 351.9 / 134.0 | 200 | G193G (GGA→GGG) | ydcV | predicted spermidine/putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (2/126); new base G (72/0); total (74/126) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 241 total aligned reads displayed.
GGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTA > REL606/1485375‑1485525 | ggATCTTGGGGCAAAGGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCagga < 1:1858150/76‑1 (MQ=255) aTCTGGGGGCCAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggagg < 2:5461793/76‑1 (MQ=255) aTCTGGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggagg < 2:5737709/76‑1 (MQ=255) tCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAggagga < 2:5692106/76‑1 (MQ=255) tCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAggagga < 1:1614064/76‑1 (MQ=255) tCTGGGGGCCAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAggagga < 1:1322836/76‑1 (MQ=255) ttGGGGCCAATGGCTGGCAAACCTTCCGCTACGTCGTGGTTCCCAATCTCAGTTCGGCGCTACTGGCcggggggga > 2:2068340/1‑73 (MQ=255) tGGGGCCAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATg < 1:572952/76‑1 (MQ=255) tGGGGCCAATGGCGGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATg < 1:2655319/76‑1 (MQ=255) aTGGCTGCCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGtt < 2:6204360/76‑1 (MQ=255) tggctggcAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGttt < 2:2648968/76‑1 (MQ=255) tggctggcAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGttt < 2:820289/76‑1 (MQ=255) tggctggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGAAAGCTGGCGgtt > 2:40384/1‑76 (MQ=255) tggctggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGttt < 2:3042352/76‑1 (MQ=255) tggcgggaAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGttt < 2:4972046/76‑1 (MQ=255) gctggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAAGGTGGGGGTTGc > 1:5005510/1‑76 (MQ=255) gctggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGc < 1:2720055/76‑1 (MQ=255) ctggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGGGTTTggc > 1:2881052/1‑74 (MQ=255) tggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGGGGTTGCCt > 2:3822402/1‑76 (MQ=255) tggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGccc > 1:4899557/1‑75 (MQ=255) tggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAAGCTGGGGTTTTCCt > 2:373767/1‑76 (MQ=255) tggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGAATGCTGGCGGTTGcgt > 2:1967063/1‑74 (MQ=255) tggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCt < 1:1736348/76‑1 (MQ=255) tggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCt < 2:1450559/76‑1 (MQ=255) ggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGGATGCTGGCGTTTGCCtt > 1:677959/1‑76 (MQ=255) gcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAGGGTGGGGGTTGCCttt > 1:3959720/1‑75 (MQ=255) gcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAAGCTGGCGGTTTTCTTg > 1:4681776/1‑76 (MQ=255) gcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTg < 1:3383560/76‑1 (MQ=255) gcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTg < 2:4239642/76‑1 (MQ=255) cAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGGTGGCGGTTGGCtttt > 2:166557/1‑74 (MQ=255) aaaCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGAAAGGCGGCGGTTGCCTTGTc > 2:1142524/1‑76 (MQ=255) aCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTTCCTTGTCGt > 1:1678439/1‑76 (MQ=255) ccTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGGAGCTGGCGGTTTGCTTgttgtt > 1:4094428/1‑76 (MQ=255) ccTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGGTTGCCTTGTCGtt > 2:5936528/1‑76 (MQ=255) ccTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGtt < 2:2038525/76‑1 (MQ=255) ccTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGtt < 2:4121750/76‑1 (MQ=255) cTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTc < 1:2472848/76‑1 (MQ=255) ttCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCg < 2:2257901/76‑1 (MQ=255) tCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCTTGTCGTTCgt > 2:5151789/1‑75 (MQ=255) cGCTACGTAGTGTTGCCTAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATg < 1:2158777/76‑1 (MQ=255) cGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGGTTGTCTTGTCGTTCGagg > 1:3067695/1‑74 (MQ=255) cTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCTTGTCGTTCGATGaa > 2:5271522/1‑76 (MQ=255) cTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAAGCTGGGGGTTGCCTTGTCGTTCGGTGaa > 1:4280345/1‑76 (MQ=255) cTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGaa < 2:6398751/76‑1 (MQ=255) tACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGaaa < 1:4167138/76‑1 (MQ=255) tACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGaaa < 1:3866062/76‑1 (MQ=255) aCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAat < 2:1612218/76‑1 (MQ=255) cGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAatc < 2:3640663/76‑1 (MQ=255) cGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAatc < 1:3720797/76‑1 (MQ=255) gTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGGTTGCCTTGTCGTTTGATGGAatca > 1:5572871/1‑76 (MQ=255) gTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGGTTGCCTTGTCGTTCGATGAAatca > 2:2448506/1‑76 (MQ=255) gTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAAGGTGGCGTTTTCCTTGTTGTTTGGTGGAatca > 1:1078503/1‑76 (MQ=255) gTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGAATGGTGGCGGTTTCCTTGTCGTTGGATGaaaaga > 1:234481/1‑73 (MQ=255) gTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAatca < 1:5936155/76‑1 (MQ=255) gTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAatca < 1:2003104/76‑1 (MQ=255) tAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCCTTTCGTTCGATGAaataat > 2:2579137/1‑76 (MQ=255) aGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGGAGGTGGGGGTTGCGTTTTCGTTTGATGAAatcatt > 1:345159/1‑75 (MQ=255) aGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGGTGGCGGTTTGCTTTTCGTTTTATGGAattatt > 1:1125250/1‑75 (MQ=255) aGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAAGCTGGGGGTTGGCTTTTTGTTTGATGAAataatc > 2:6225844/1‑76 (MQ=255) gtgtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCg < 1:5098146/76‑1 (MQ=255) tgtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGGCGGAGCTTTCCTTGTCTTTCGTTGATATCCTCGt > 1:6332766/1‑76 (MQ=255) gtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTTTCCTTTCGTTCGATGGAATAATCGtt > 1:1410169/1‑76 (MQ=255) gtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGGTTGCCTTGTCGTTCGATGAAATAATCGtt > 2:4809960/1‑76 (MQ=255) gtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGtt < 2:1923413/76‑1 (MQ=255) gtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGtt < 1:5067169/76‑1 (MQ=255) ttGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTTCCTTGTCGTTCGATGAAATCATCGTTa > 1:4378522/1‑76 (MQ=255) ttGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTa > 2:519359/1‑76 (MQ=255) tGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTAc < 2:5946495/76‑1 (MQ=255) tGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTAc < 1:3265056/76‑1 (MQ=255) tGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTAc < 2:5665678/76‑1 (MQ=255) gCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACg < 1:5552546/76‑1 (MQ=255) aaTCTCAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTTCGACCt > 2:6554735/1‑76 (MQ=255) ctcAGTTCGGCGTTACTGGCAGGGGGGATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCtttt > 1:6307853/1‑75 (MQ=255) ctcAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTa < 1:6438394/76‑1 (MQ=255) ctcAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTa < 1:1798131/76‑1 (MQ=255) ctcAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTa < 2:1292578/76‑1 (MQ=255) tcAGTTCGGCGTTACTGGCAGGGGGGATGGTGGGGTTTGTCTTGTTGTTCGATGAAATAATCGTTACTACCttttc > 2:4947201/1‑74 (MQ=255) tcAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTAc < 1:3004120/76‑1 (MQ=255) tcAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTAc < 2:4478227/76‑1 (MQ=255) cAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACg < 1:4812143/76‑1 (MQ=255) cAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACg < 1:4194068/76‑1 (MQ=255) aGTTCGGCGTTACTGGCAGGGGGAATGCTGGGGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGAACTTTTCgg > 1:5160235/1‑76 (MQ=255) ttCGGCGTTACTGGCAGGGGGGATGCTGGCGGTTGCCCTGTTGTTACTTGAAATCAACGTTACGACCTTTACCGCa > 1:2531454/1‑76 (MQ=255) tCGGCGTTACTGGCAGGGGGGATGCTGGGGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTATGGCAg > 2:5498275/1‑76 (MQ=255) tCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAg < 1:1192019/76‑1 (MQ=255) cGGCGTTACTGGCAGGGGGGAAGCTGGCGTTTTGCTTGTCGTTCGATGAAATCATCGTTACGACCTTTTCGGCAgg > 2:4724714/1‑76 (MQ=255) cGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCa < 1:5323241/76‑1 (MQ=255) ttACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATg < 2:4334962/76‑1 (MQ=255) tACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATga < 2:3498000/76‑1 (MQ=255) tACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATga < 2:131009/76‑1 (MQ=255) aCTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaa < 2:75929/76‑1 (MQ=255) cTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaac < 2:2873164/76‑1 (MQ=255) cTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaac < 2:6942410/76‑1 (MQ=255) tGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGGGGTgaacg > 1:4364275/1‑76 (MQ=255) ggCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacga < 2:2027666/76‑1 (MQ=255) gCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaa < 2:1070043/76‑1 (MQ=255) cAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaac < 2:2994858/76‑1 (MQ=255) cAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaac < 1:6744543/76‑1 (MQ=255) aggaggAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCCTgaacgaaca > 2:3177860/1‑75 (MQ=255) gaggaATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGtt < 1:864251/76‑1 (MQ=255) | GGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTA > REL606/1485375‑1485525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |