| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 124,938 | A→G | I15V (ATT→GTT) | pdhR → | transcriptional regulator of pyruvate dehydrogenase complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 124,938 | 0 | A | G | 97.3% | 777.6 / 6.6 | 188 | I15V (ATT→GTT) | pdhR | transcriptional regulator of pyruvate dehydrogenase complex |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base G (104/79); total (106/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01 | |||||||||||
Only 100 of 190 total aligned reads displayed.
GACTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGATTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAAC > REL606/124865‑125013 | gACTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGtt < 2:2780521/76‑1 (MQ=255) aCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTg < 1:4870839/76‑1 (MQ=255) aCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTg < 1:1740572/76‑1 (MQ=255) aCTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTg < 1:6048035/76‑1 (MQ=255) cTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGa > 1:2934733/1‑76 (MQ=255) ttCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGag > 2:2363235/1‑76 (MQ=255) ttCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGag < 2:4813221/76‑1 (MQ=255) ttCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGag > 1:2024518/1‑76 (MQ=255) ttCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGag < 2:4982009/76‑1 (MQ=255) ttCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGag > 2:5885/1‑76 (MQ=255) tCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGagc < 1:398036/76‑1 (MQ=255) gCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGagcagc > 1:1382326/1‑76 (MQ=255) gCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGagcagc > 2:5487632/1‑76 (MQ=255) cAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAgcagca > 2:1867645/1‑76 (MQ=255) cAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAgcagca < 1:2129806/76‑1 (MQ=255) aaGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCaa > 1:5306337/1‑76 (MQ=255) aGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAAc > 1:3451675/1‑76 (MQ=255) gTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACt > 1:692060/1‑76 (MQ=255) gTGGCTAAAGACAGGAACTCAGGCCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACt < 2:2207620/76‑1 (MQ=255) ggCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTgg < 2:6761496/76‑1 (MQ=255) gCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGa > 2:5671484/1‑76 (MQ=255) ttAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGt > 2:1633654/1‑76 (MQ=255) ttAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGt > 1:6764898/1‑76 (MQ=255) ttAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGt > 1:4464286/1‑76 (MQ=255) ttAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGt > 1:366167/1‑76 (MQ=255) tAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGtt > 2:292515/1‑76 (MQ=255) aGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGtttt > 1:5338287/1‑76 (MQ=255) gACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGCGCAGCAACTGGAGttttt > 1:2843175/1‑76 (MQ=255) aCAGGAACTCAGGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGATTGAGCAGCAACTGGAGTTTTTg < 1:2717978/76‑1 (MQ=255) ggACCTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATc < 2:4596852/76‑1 (MQ=255) gAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATcc < 2:6687626/76‑1 (MQ=255) aaCTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGATTGAGCAGCAACTGGAGTTTTTGATCCt < 2:5995077/76‑1 (MQ=255) aCTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTc > 2:6878461/1‑76 (MQ=255) aCTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTc < 2:6362957/76‑1 (MQ=255) cTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCg < 1:4183438/76‑1 (MQ=255) cTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCg < 2:4753376/76‑1 (MQ=255) tCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGa > 1:3758439/1‑76 (MQ=255) tCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGa > 2:5641827/1‑76 (MQ=255) tCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGa > 1:1498513/1‑76 (MQ=255) cATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGaa > 1:4734365/1‑76 (MQ=255) cATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGaa < 2:1397699/76‑1 (MQ=255) aTGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAg > 1:2519583/1‑76 (MQ=255) tGGCCTACAGAAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAgg < 2:2524568/76‑1 (MQ=255) ggCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGc > 2:3180238/1‑76 (MQ=255) ggCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGc > 1:1292227/1‑76 (MQ=255) gCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCa > 2:6623011/1‑76 (MQ=255) ccTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCAc > 2:1066153/1‑76 (MQ=255) ccTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCAc > 2:4251256/1‑76 (MQ=255) ccTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCAc > 1:2904100/1‑76 (MQ=255) cTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCAct > 1:5514774/1‑76 (MQ=255) cTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCAct < 1:2778562/76‑1 (MQ=255) tACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCActc > 1:4717871/1‑76 (MQ=255) aCAGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCActct > 2:3797248/1‑76 (MQ=255) aCAGCAAAACCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCActct < 2:4903031/76‑1 (MQ=255) aGCAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTcc > 2:1692634/1‑76 (MQ=255) gCAAAACCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCg < 1:6576406/76‑1 (MQ=255) cAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGc < 1:4370989/76‑1 (MQ=255) cAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGc < 2:3129386/76‑1 (MQ=255) cAAAATCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGc < 2:2689396/76‑1 (MQ=255) aaaaTCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGcc > 2:2601895/1‑76 (MQ=255) aacccGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCg < 2:22764/73‑1 (MQ=255) aaTCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCg > 1:232940/1‑76 (MQ=255) aTCCGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCgg > 1:1219782/1‑76 (MQ=255) aTCCCCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCgg < 1:649194/76‑1 (MQ=255) cGCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCg < 1:948597/76‑1 (MQ=255) gCCAACCAAAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGa > 2:3298360/1‑76 (MQ=255) ccaaccaaACCTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGaa < 2:3577728/76‑1 (MQ=255) ccaaccaaAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGaa > 2:1437232/1‑76 (MQ=255) caaccaaAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGaaa < 2:418236/76‑1 (MQ=255) aaccaaAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGaaaa < 2:1589243/76‑1 (MQ=255) accaaAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGaaaaa < 2:2343885/76‑1 (MQ=255) ccaaACCTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCCCTCTCCGCCCGGGCGAAAAAc < 1:5560395/76‑1 (MQ=255) caaAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACt > 2:6440113/1‑76 (MQ=255) caaAACTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACt > 1:3651871/1‑76 (MQ=255) aaaaCTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTc > 1:5919824/1‑76 (MQ=255) aaaCTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTcc > 1:1807545/1‑76 (MQ=255) aCTCTCCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCa > 2:1985725/1‑76 (MQ=255) tctcCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCAcc > 2:4039765/1‑76 (MQ=255) tctcCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCAcc < 2:1768058/76‑1 (MQ=255) ctcCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCg > 1:3935833/1‑76 (MQ=255) ctcCGATGTGGTTGACCAGCAACTGGAGTTTTTGATCCTCGAAGACACTCTCCGCCCGGGCGAAAAACTCCCACCg < 2:1211003/76‑1 (MQ=255) tcCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCgg < 2:3656459/76‑1 (MQ=255) tcCGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCgg > 2:1203805/1‑76 (MQ=255) tcCGATGGGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCgg < 1:3578003/76‑1 (MQ=255) ccGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGa < 2:5846397/76‑1 (MQ=255) ccGATGTGATTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGa > 1:164688/1‑76 (MQ=255) cGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGaa > 1:229959/1‑76 (MQ=255) cGATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGaa > 2:45963/1‑76 (MQ=255) gATGTGGTTGAGCAGCAACTTGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAAc < 2:3437969/76‑1 (MQ=255) gATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAAc > 2:5191273/1‑76 (MQ=255) gATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAAc < 1:5835143/76‑1 (MQ=255) gATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAAc > 1:816581/1‑76 (MQ=255) gATGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAAc < 1:1959772/76‑1 (MQ=255) aTGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAGCTCCCACCGGAAcg < 2:932739/76‑1 (MQ=255) aTGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAAcg < 2:1838377/76‑1 (MQ=255) aTGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAAcg > 2:4002143/1‑76 (MQ=255) aTGTGGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAAcg > 1:4567653/1‑76 (MQ=255) gtgGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAAcgcg > 1:3867411/1‑76 (MQ=255) gtgGTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAAcgcg > 2:4456804/1‑76 (MQ=255) aTTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAAc < 1:5008715/76‑1 (MQ=255) | GACTTCGGCAAGTGGCTTAAGACAGGAACTCATGGCCTACAGCAAAATCCGCCAACCAAAACTCTCCGATGTGATTGAGCAGCAACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAAC > REL606/124865‑125013 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |