| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,655,307 | T→C | N92S (AAT→AGT) | clpB ← | protein disaggregation chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,655,307 | 0 | T | C | 99.5% | 923.6 / ‑4.4 | 216 | N92S (AAT→AGT) | clpB | protein disaggregation chaperone |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (103/112); total (104/112) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
Only 100 of 219 total aligned reads displayed.
AAGTGCCGCCAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGATTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGTAAACGATTTA > REL606/2655234‑2655379 | aGTGCCGCCAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTaa < 2:6960254/76‑1 (MQ=255) aGTGCCGCCAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTaa < 2:3537029/76‑1 (MQ=255) gTGCCGCCAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAg < 2:5670537/76‑1 (MQ=255) gTGCCGCCAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAg < 1:3869646/76‑1 (MQ=255) gTGCCGCCAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAg < 2:6354529/76‑1 (MQ=255) gTGCCGCCAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAg > 1:2372204/1‑76 (MQ=255) tGCCGCCAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGa > 2:3815922/1‑76 (MQ=255) tGCCGCCAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGa < 2:731857/76‑1 (MQ=255) tGCCGCCAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGa < 2:5363374/76‑1 (MQ=255) tGCCGCCAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGa < 1:2852722/76‑1 (MQ=255) ccgccAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAAc < 1:6272539/76‑1 (MQ=255) cgccAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAAcg < 2:3112523/76‑1 (MQ=255) cgccAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAAcg < 2:6558649/76‑1 (MQ=255) cAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACgcgc > 2:6052700/1‑76 (MQ=255) cAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACgcgc > 2:5034465/1‑76 (MQ=255) aGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCa > 1:3750143/1‑76 (MQ=255) aGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCa > 1:5281050/1‑76 (MQ=255) acgaacAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCa < 2:1987421/76‑1 (MQ=255) gaacAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGa < 2:5773651/76‑1 (MQ=255) gaacAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGa < 1:5034504/76‑1 (MQ=255) aacAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGAt > 1:655830/1‑76 (MQ=255) aacAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGAt < 1:4476539/76‑1 (MQ=255) acAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATc < 2:1440273/76‑1 (MQ=255) aGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCt > 1:6133444/1‑76 (MQ=255) aGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCt < 2:4939674/76‑1 (MQ=255) ttCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCtgt > 1:240369/1‑76 (MQ=255) ttCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCtgt > 2:8677/1‑76 (MQ=255) ttCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCtgt > 1:3436886/1‑76 (MQ=255) tCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCtgtg > 2:4060083/1‑76 (MQ=255) tGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGAt < 1:4223217/76‑1 (MQ=255) tGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGAt > 2:3348885/1‑76 (MQ=255) gACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATg < 1:35971/76‑1 (MQ=255) gACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATg < 2:4263403/76‑1 (MQ=255) gACGAGATAAAGTTAGCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATg < 1:1571791/76‑1 (MQ=255) aCGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATgg > 2:4213984/1‑76 (MQ=255) agagaTAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGc < 1:2024768/75‑1 (MQ=255) gagaTAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCt > 2:2291224/1‑76 (MQ=255) agaTAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTg > 2:539979/1‑76 (MQ=255) gaTAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTgg > 2:3709570/1‑76 (MQ=255) aTAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGa < 1:2911803/76‑1 (MQ=255) tAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGAc > 1:5244605/1‑76 (MQ=255) aaaGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACa < 1:100496/76‑1 (MQ=255) aaaGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACa > 2:6439797/1‑76 (MQ=255) aaGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACAt > 1:6856914/1‑76 (MQ=255) aaGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACAt < 2:1740672/76‑1 (MQ=255) gTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATca > 2:515620/1‑76 (MQ=255) gTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATca < 1:4289060/76‑1 (MQ=255) gTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATca < 2:2859608/76‑1 (MQ=255) ttATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATcac > 2:4807634/1‑76 (MQ=255) ttATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATcac > 2:4455140/1‑76 (MQ=255) ttATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATcac > 1:6731784/1‑76 (MQ=255) tATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATcacc < 1:5902740/76‑1 (MQ=255) tATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATcacc > 2:6736714/1‑76 (MQ=255) tATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATcacc > 2:3020666/1‑76 (MQ=255) aTCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATcacca < 1:6009151/76‑1 (MQ=255) aTCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATcacca < 1:1700201/76‑1 (MQ=255) tCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATcaccac < 2:3981651/76‑1 (MQ=255) tCACCACGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATcaccac > 2:5273325/1‑76 (MQ=255) caccacGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATTaccacc > 1:5752102/1‑76 (MQ=255) caccacGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCaccacc > 1:1110882/1‑76 (MQ=255) caccacGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCaccacc > 1:4621287/1‑76 (MQ=255) caccacGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCaccacc > 2:1261229/1‑76 (MQ=255) cacGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGt < 2:3442164/76‑1 (MQ=255) cacGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGt > 1:5583998/1‑76 (MQ=255) cGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTAc < 2:4538620/76‑1 (MQ=255) cGTTTTTGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTAc < 1:730261/76‑1 (MQ=255) ttttGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCtt < 2:5737826/76‑1 (MQ=255) tttGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTc > 2:2561836/1‑76 (MQ=255) tttGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTc > 2:5865186/1‑76 (MQ=255) tttGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTc > 1:308755/1‑76 (MQ=255) ttGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCa > 1:2729537/1‑76 (MQ=255) ttGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCGGGACATCACCACCAGTACCTTCa < 2:723031/76‑1 (MQ=255) tGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCaa < 2:5426156/76‑1 (MQ=255) tGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCaa < 1:276494/76‑1 (MQ=255) tGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCaa > 1:982007/1‑76 (MQ=255) tGCGCCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCaa < 2:1776280/76‑1 (MQ=255) cgcCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAAcc > 1:1289268/1‑76 (MQ=255) gcCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCt < 2:1363154/76‑1 (MQ=255) gcCAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCt < 1:3709396/76‑1 (MQ=255) cAGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGc < 1:335631/76‑1 (MQ=255) aGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCg > 2:6838256/1‑76 (MQ=255) aGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCg < 1:1174486/76‑1 (MQ=255) aGCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCg > 2:2257275/1‑76 (MQ=255) gCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCgg > 2:169087/1‑76 (MQ=255) gCTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCgg > 2:2166458/1‑76 (MQ=255) cTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGt < 2:2783698/76‑1 (MQ=255) cTTGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGt > 1:4712093/1‑76 (MQ=255) ttGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGTa > 1:3965503/1‑76 (MQ=255) tGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGTaa < 2:3197605/76‑1 (MQ=255) tGTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGTaa < 1:2140564/76‑1 (MQ=255) gTCGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGTaaa < 2:5990938/76‑1 (MQ=255) cGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGTAAACg > 1:4081125/1‑76 (MQ=255) cGCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGTAAACg > 1:2241646/1‑76 (MQ=255) gCAAAGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGTAAACGa < 1:4633463/76‑1 (MQ=255) aaaGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGTAAACGAtt < 1:5110301/76‑1 (MQ=255) aaaGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGCAAACGAtt > 2:5590673/1‑76 (MQ=255) aaGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGTAAACGAttt < 1:540650/76‑1 (MQ=255) aaGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGTAAACGAttt > 2:5537186/1‑76 (MQ=255) aGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCGCCACCAGTACCTTCAACCTGCGGTAAACGATTTa < 2:6704097/76‑1 (MQ=255) aGACTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGTAAACGATTTa < 1:78943/76‑1 (MQ=255) | AAGTGCCGCCAGAACGAACAGTTCTGACGAGATAAAGTTATCACCACGTTTTTGCGCCAGCTTGTCGCAAAGATTAAGAACGCGCACCAGATCCTGTGATGGCTGGACATCACCACCAGTACCTTCAACCTGCGGTAAACGATTTA > REL606/2655234‑2655379 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |