| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,163,752 | T→A | I966N (ATC→AAC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,163,752 | 0 | T | A | 99.3% | 566.9 / NA | 139 | I966N (ATC→AAC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (99/39); minor base C (0/1); total (99/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 163 total aligned reads displayed.
ACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA > REL606/4163680‑4163824 | aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCt < 1:737540/76‑1 (MQ=255)aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCt < 1:5859982/76‑1 (MQ=255)aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCt > 2:305912/1‑76 (MQ=255)aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCt > 2:467843/1‑76 (MQ=255)aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCt > 2:6209237/1‑76 (MQ=255) cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTc < 2:329268/76‑1 (MQ=255) aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCg > 1:1738322/1‑76 (MQ=255) aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCg > 2:5911852/1‑76 (MQ=255) aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGa < 2:4973054/76‑1 (MQ=255) aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGaa < 1:982675/76‑1 (MQ=255) aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGaa < 2:5002229/76‑1 (MQ=255) gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGc > 2:5719504/1‑76 (MQ=255) tGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCg > 2:2029460/1‑76 (MQ=255) tGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCg > 1:3068113/1‑76 (MQ=255) cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCggg > 2:1490539/1‑76 (MQ=255) tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCt > 2:6126390/1‑76 (MQ=255) tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCCCGAAGCGGGTCt < 1:5080661/76‑1 (MQ=255) ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTg > 1:4321064/1‑76 (MQ=255) ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTg < 1:4511025/76‑1 (MQ=255) ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTg < 2:6527501/76‑1 (MQ=255) ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTg > 1:161464/1‑76 (MQ=255) ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGACTg > 1:1363147/1‑76 (MQ=255) aaaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGtt < 2:2030234/76‑1 (MQ=255) aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑c > 2:2163987/1‑76 (MQ=255) aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑Ca < 2:2465213/76‑1 (MQ=255) tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAg > 1:3998458/1‑76 (MQ=255) tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAg < 1:1775053/76‑1 (MQ=255) cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGc > 2:1808841/1‑76 (MQ=255) cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGc > 1:4459197/1‑76 (MQ=255) gaagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGcc > 1:1178633/1‑76 (MQ=255) gaagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGcc > 2:4338508/1‑76 (MQ=255) gaagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGcc > 2:468884/1‑76 (MQ=255) agaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGt < 1:5223446/76‑1 (MQ=255) agaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGt < 2:6706061/76‑1 (MQ=255) gaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTa > 1:1868137/1‑76 (MQ=255) aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTAt > 2:4507255/1‑76 (MQ=255) aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTAt < 2:2274776/76‑1 (MQ=255) aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTAt < 2:5143787/76‑1 (MQ=255) aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTAt > 2:6474577/1‑76 (MQ=255) aTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATcc < 2:3014997/76‑1 (MQ=255) tGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCg > 1:4158933/1‑76 (MQ=255) gCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCCGTT‑CAGCCGTATACgt > 1:1440477/1‑76 (MQ=255) aGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgc > 1:111657/1‑76 (MQ=255) gCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgct > 2:4810953/1‑76 (MQ=255) gCTAAAACAGGCGAAGAAAGACCTGTCTAAAAACCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgct < 2:1808016/76‑1 (MQ=255) cAAACAGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgtg < 1:2042893/76‑1 (MQ=255) cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgtg > 2:874041/1‑76 (MQ=255) aaaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgtgc > 1:1771044/1‑76 (MQ=255) aCAGGCGAAGAAAGCCCTGTCTGAAGAAGTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGtgctgtgctg < 2:4464277/76‑1 (MQ=255) aCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGtgctgtgctg < 2:3064051/76‑1 (MQ=255) aCAGGCGAAGAAAGACCTGTCTGAAGAACGGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCTGtgctgtgctg > 2:6959408/1‑76 (MQ=255) cAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCCCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTgg > 1:191988/1‑76 (MQ=255) aGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGt > 2:2026799/1‑76 (MQ=255) gggaaaaaAGACCTTTCTGAAGAACTGCAGAACCTCAAAGCGGTTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAg < 2:1127364/74‑1 (MQ=255) cGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGc > 2:2271965/1‑76 (MQ=255) gaagaaAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGcc > 2:5013960/1‑76 (MQ=255) aagaaagaCCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCg > 1:2157255/1‑76 (MQ=255) aaaaaagcCTTGTCGAAAGACCTGAAGACCCTCGAGGCGGTTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCg < 2:4654992/73‑1 (MQ=255) agaaagcCCTGTCTGAAGACCTGAAGACCCTGAAAGCGGGTCTTTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCgg < 2:3561820/76‑1 (MQ=255) aaaaagcCCGGTCTGAAGAACTGAAGACCCTCGAAGGGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCgg < 2:4875570/74‑1 (MQ=255) aaagcCCTGTCTGAAGACCTGCAGACCCTCGAAGGGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg < 1:2450979/76‑1 (MQ=255) aaagaCCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCggcg > 2:4118648/1‑74 (MQ=255) aagcCCTGTCGGAAAACCTGCAAACCCGCGAGGGGGGTCTTTC‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg < 2:326341/76‑1 (MQ=255) aagcCCTGTCGAAAAAACTGCAGACCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg < 1:466110/76‑1 (MQ=255) aagaCCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg > 1:4623968/1‑76 (MQ=255) aagaCCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg > 2:238467/1‑76 (MQ=255) gcccTGTCGGAAAATCTGCAGACCCTCGACGGGGTTTTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg < 1:6047796/74‑1 (MQ=255) cTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTg > 1:4256677/1‑76 (MQ=255) cTGTCTGAAAACCGGCAGACCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTg < 1:5974215/76‑1 (MQ=255) tGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGa > 1:1694547/1‑76 (MQ=255) gTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa > 2:1436886/1‑76 (MQ=255) tCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg > 1:4296079/1‑76 (MQ=255) tCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg > 2:6793500/1‑76 (MQ=255) cTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc > 2:72328/1‑76 (MQ=255) cTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc > 1:3689041/1‑76 (MQ=255) cTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc > 1:2763545/1‑76 (MQ=255) tGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt > 1:5361570/1‑76 (MQ=255) gaagaaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg > 2:2805556/1‑76 (MQ=255) gaagaaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg > 2:1717388/1‑76 (MQ=255) aaagaaCTGCAGACCCTCGAGGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg < 1:3420257/75‑1 (MQ=255) aagacCTGCAAACCCGCAAAGCGGGTCTTTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga < 1:2625113/76‑1 (MQ=255) aagaaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga > 1:1328426/1‑76 (MQ=255) aagaaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga > 1:2755612/1‑76 (MQ=255) aagaaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGAGCTGGTAGCCGGTGGCGTTGGAGCTga > 1:6440014/1‑76 (MQ=255) agaaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag > 2:4576038/1‑76 (MQ=255) aaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgagca > 2:5986324/1‑74 (MQ=255) aaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGaa > 1:6542140/1‑76 (MQ=255) aCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAg > 2:3947561/1‑76 (MQ=255) tggaGAACCTCCAAGCGGGTTTTTTCCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGc < 2:3766237/73‑1 (MQ=255) tGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt > 2:5520690/1‑76 (MQ=255) tGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGGGGCGTTGCAGCTGAGAAGCt > 2:2184749/1‑76 (MQ=255) gCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc > 1:6036279/1‑76 (MQ=255) gCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc > 1:4651638/1‑76 (MQ=255) cAGACCCTGGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg < 1:3019571/76‑1 (MQ=255) cAGACCCCCGAGGCGGGTCTGTC‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg < 1:383458/76‑1 (MQ=255) cAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg > 2:5461109/1‑76 (MQ=255) aGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGCGAAGCTCGa > 1:3349874/1‑76 (MQ=255) aGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa > 1:5727695/1‑76 (MQ=255) aGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa > 1:5736124/1‑76 (MQ=255) aGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa > 2:2911668/1‑76 (MQ=255) | ACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA > REL606/4163680‑4163824 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |