| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 433,525 | T→A | I7N (ATC→AAC) | hupB → | HU, DNA‑binding transcriptional regulator, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 433,525 | 0 | T | A | 98.1% | 858.4 / 4.9 | 208 | I7N (ATC→AAC) | hupB | HU, DNA‑binding transcriptional regulator, beta subunit |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base A (119/85); total (121/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
Only 100 of 212 total aligned reads displayed.
GTTCGTACCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTC > REL606/433452‑433597 | gTTCGTACCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGATCg < 2:5338695/76‑1 (MQ=255) ttCGTACCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGa > 1:4494246/1‑76 (MQ=255) ttCGTACCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGa > 2:5826500/1‑76 (MQ=255) cGTACCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACa < 2:4970141/76‑1 (MQ=255) cGTACCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACa < 1:3470154/76‑1 (MQ=255) cGTACCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACa < 1:6478298/76‑1 (MQ=255) gTACCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACaa < 1:5742689/76‑1 (MQ=255) gTACCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACaa < 2:1248406/76‑1 (MQ=255) tACCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAg > 2:2546014/1‑76 (MQ=255) tACCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAg < 1:2730729/76‑1 (MQ=255) aCCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGa < 2:1956617/76‑1 (MQ=255) aCCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGa < 1:154198/76‑1 (MQ=255) aCCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGa > 2:3286581/1‑76 (MQ=255) cTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGAtt < 2:1756832/76‑1 (MQ=255) cTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGAtt > 2:5097373/1‑76 (MQ=255) ttGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATtg < 1:2656970/76‑1 (MQ=255) ttGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATtg > 1:898430/1‑76 (MQ=255) gAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATtgct > 1:175755/1‑76 (MQ=255) aGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATtgctgc > 2:6778964/1‑76 (MQ=255) ggATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCa < 1:5149988/76‑1 (MQ=255) gATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGATCGACAAGATTGCTGCAg > 2:6497732/1‑76 (MQ=255) gATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAg < 2:4682356/76‑1 (MQ=255) aTTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAgg > 1:776304/1‑76 (MQ=255) ttCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAggg > 1:591881/1‑76 (MQ=255) tCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAgggg < 1:6051429/76‑1 (MQ=255) cAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGc > 1:5269447/1‑76 (MQ=255) aaGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCt > 1:6125860/1‑76 (MQ=255) aaGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCt > 1:5675663/1‑76 (MQ=255) tGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGat > 1:3281262/1‑76 (MQ=255) tGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGat < 1:4797304/76‑1 (MQ=255) gCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGata < 1:3225514/76‑1 (MQ=255) gCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGata > 1:3761658/1‑76 (MQ=255) atatAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATAtct < 1:4890252/76‑1 (MQ=255) atatAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATAtct > 2:5242838/1‑76 (MQ=255) ataAATTAAAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATctct < 1:3820499/76‑1 (MQ=255) taAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTa > 2:6762302/1‑76 (MQ=255) taAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTa < 1:4287441/76‑1 (MQ=255) aaaTTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTaa > 1:6745300/1‑76 (MQ=255) aaTTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTaaa > 2:3453393/1‑76 (MQ=255) aaTTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTaaa < 1:1864141/76‑1 (MQ=255) aTTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAg < 1:5097373/76‑1 (MQ=255) aTTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAg < 1:3627547/76‑1 (MQ=255) tataAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCt > 1:2709834/1‑76 (MQ=255) ataAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTg > 2:199886/1‑76 (MQ=255) ataAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTg < 2:6566063/76‑1 (MQ=255) ataAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTg < 2:5029594/76‑1 (MQ=255) aaaGCGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCg > 2:1171775/1‑76 (MQ=255) aaaGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCg > 2:6648581/1‑76 (MQ=255) aaaGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCg > 1:5046470/1‑76 (MQ=255) aaaGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCg > 2:1016363/1‑76 (MQ=255) aaaGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCg > 2:2950313/1‑76 (MQ=255) aaGAGGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCgg > 1:1270242/1‑76 (MQ=255) gagGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAACCTGCGGCt < 2:6864211/76‑1 (MQ=255) agGAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTg > 2:5144347/1‑76 (MQ=255) ggAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTgg > 1:2329001/1‑76 (MQ=255) ggAAGAGAAGAGTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTgg > 2:3396427/1‑76 (MQ=255) aagagaagagTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATTTCTAAAGCTGCGGCTGGcc > 2:4186102/1‑76 (MQ=255) aagagaagagTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGcc > 1:5864736/1‑76 (MQ=255) aagagaagagTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGcc > 2:6463723/1‑76 (MQ=255) agagaagagTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGGCg > 1:4582419/1‑76 (MQ=255) agagaagagTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCg < 2:520369/76‑1 (MQ=255) agagaagagTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCg < 2:6063307/76‑1 (MQ=255) agaagagTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCggg > 2:1364411/1‑74 (MQ=255) aagagTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGGGCg > 2:5514721/1‑76 (MQ=255) agagTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGt < 2:6307990/76‑1 (MQ=255) agagTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGGGCGt > 2:2899684/1‑76 (MQ=255) gagTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGtt < 1:4687279/76‑1 (MQ=255) gagTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGtt < 1:5634893/76‑1 (MQ=255) gagTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGtt < 2:1120084/76‑1 (MQ=255) gagTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGtt < 1:2729121/76‑1 (MQ=255) agTGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTa < 2:2342791/76‑1 (MQ=255) tGAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGGCGTGCGGTAGa > 2:1860389/1‑76 (MQ=255) gAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGAt < 1:1581969/76‑1 (MQ=255) gAATAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGAt < 1:1345684/76‑1 (MQ=255) tAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCt > 2:2226718/1‑76 (MQ=255) tAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCt < 1:2107340/76‑1 (MQ=255) tAAATCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCt > 1:6302558/1‑76 (MQ=255) aaaTCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGGCGTGCGTTAGATGCta > 1:2859398/1‑76 (MQ=255) aaaTCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCta < 2:2152894/76‑1 (MQ=255) aaTCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCtat < 1:5106423/76‑1 (MQ=255) aaTCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCtat < 2:5577837/76‑1 (MQ=255) aaTCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGGTAGATGCtat > 2:4427472/1‑76 (MQ=255) aaTCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGGGCGGTAGATGCtat > 1:257432/1‑76 (MQ=255) aTCTCAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCtatt < 1:2015923/76‑1 (MQ=255) aTCTCAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGGGCGTTAGATGCtatt > 1:1411848/1‑76 (MQ=255) tctcAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGGCGGGCGTTTGATGCtattt > 1:6115255/1‑75 (MQ=255) tctcAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCtatta < 1:4328270/76‑1 (MQ=255) gctcAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCtatta < 2:5743276/75‑1 (MQ=255) ctcAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTTGATGCtattat > 1:3604896/1‑76 (MQ=255) ctcAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCtattat < 1:4954795/76‑1 (MQ=255) tcAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTTGGAGCTattatt > 1:3332163/1‑76 (MQ=255) cAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTattattt > 1:6487216/1‑75 (MQ=255) cAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTg > 1:987956/1‑76 (MQ=255) cAATTGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTg < 1:1094291/76‑1 (MQ=255) ttGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCtt < 2:6117691/76‑1 (MQ=255) ttGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCtt < 2:1587452/76‑1 (MQ=255) ttGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCtt < 2:325585/76‑1 (MQ=255) ttGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCtt < 2:3704252/76‑1 (MQ=255) ttGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCtt < 2:4884759/76‑1 (MQ=255) tGAACGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGGTAGATGCTATTATTGCTTc > 2:4009107/1‑76 (MQ=255) | GTTCGTACCTTGAAGGATTCAAGTGCGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATCTCAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTC > REL606/433452‑433597 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |