BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	475,161	C→A	G49C (GGC→TGC)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	475,161	0	C	A	100.0%	640.4 / NA	164	G49C (GGC→TGC)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (98/66); total (98/66)

Only 100 of 213 total aligned reads displayed.

CCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTG  >  REL606/475088‑475231

ccGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACg                                                                      >  2:6934161/1‑76 (MQ=255)

ccGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACg                                                                      >  2:1189528/1‑76 (MQ=255)

ccGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACg                                                                      >  1:4639114/1‑76 (MQ=255)

 cGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACgg                                                                     <  1:1852748/76‑1 (MQ=255)

 cGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACgg                                                                     <  1:4478194/76‑1 (MQ=255)

  gACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGa                                                                    <  2:4668584/76‑1 (MQ=255)

  gACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGa                                                                    <  2:1866468/76‑1 (MQ=255)

    cGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGt                                                                  <  2:2077720/76‑1 (MQ=255)

    cGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGt                                                                  >  1:5744095/1‑76 (MQ=255)

     gCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTg                                                                 >  1:1607527/1‑76 (MQ=255)

     gCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTg                                                                 <  1:3844459/76‑1 (MQ=255)

      ccAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGa                                                                <  1:1174402/76‑1 (MQ=255)

      ccAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGa                                                                >  1:4373802/1‑76 (MQ=255)

       cAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGaa                                                               >  1:2050422/1‑76 (MQ=255)

       cAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGaa                                                               >  1:4094609/1‑76 (MQ=255)

       cAAACATCAACAGAATAACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGaa                                                               >  2:1144987/1‑76 (MQ=255)

         aaCATCTACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATg                                                             >  2:3852039/1‑76 (MQ=255)

         aaCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATg                                                             >  2:1745433/1‑76 (MQ=255)

           cATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGt                                                           >  2:6342515/1‑76 (MQ=255)

            aTCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTc                                                          <  2:6214667/76‑1 (MQ=255)

            aTCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTc                                                          >  1:6453830/1‑76 (MQ=255)

             tCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGGcc                                                         >  1:5627491/1‑76 (MQ=255)

              cAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCt                                                        >  2:4052306/1‑76 (MQ=255)

              cAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCt                                                        >  1:2693684/1‑76 (MQ=255)

              cAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCt                                                        >  1:613239/1‑76 (MQ=255)

              cAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCt                                                        >  1:3175466/1‑76 (MQ=255)

              cAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGGCCt                                                        >  1:1219947/1‑76 (MQ=255)

               aaCAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTg                                                       <  2:1079119/76‑1 (MQ=255)

               aaCAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTg                                                       <  1:2440528/76‑1 (MQ=255)

                  aGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCa                                                    <  1:1318195/76‑1 (MQ=255)

                  aGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCa                                                    >  2:3898698/1‑76 (MQ=255)

                   gAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAg                                                   <  2:2949541/76‑1 (MQ=255)

                   gAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAg                                                   >  1:5891504/1‑76 (MQ=255)

                   gAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAg                                                   <  1:3157703/76‑1 (MQ=255)

                    aaTGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTTCCAGc                                                  >  2:49739/1‑76 (MQ=255)

                      tGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGccc                                                >  2:6535780/1‑74 (MQ=255)

                      tGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGcac                                                >  2:3571938/1‑76 (MQ=255)

                      ggACGCCCGGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGcac                                                <  1:1492444/75‑1 (MQ=255)

                       gccGCCCAGTCCAGCCAGTTCGGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGCCCTGCCAGcaca                                               <  2:6166046/74‑1 (MQ=255)

                        acccccGGTTCAGCCAGTTCCGGGGAAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCacac                                              <  1:1874948/73‑1 (MQ=255)

                        aCGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCacac                                              >  1:6267231/1‑76 (MQ=255)

                          gCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGGCCTGCCAGCACAccc                                            >  2:2049188/1‑76 (MQ=255)

                          gCCCAGTCCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACAccc                                            <  2:3346309/76‑1 (MQ=255)

                            caaGTCCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGc                                          <  2:2594272/74‑1 (MQ=255)

                             cggTTCAGCCAGTGCCGGGGAAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCt                                         <  2:4544627/74‑1 (MQ=255)

                             cAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCt                                         >  1:6060411/1‑76 (MQ=255)

                             cAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCt                                         >  2:2400154/1‑76 (MQ=255)

                             cAGTTCAGCCAGTTCCGGGGCAAGCTGGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCt                                         <  2:1648318/76‑1 (MQ=255)

                              aGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTa                                        >  1:35588/1‑76 (MQ=255)

                               gTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTaa                                       <  1:5512631/76‑1 (MQ=255)

                               gTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTaa                                       >  1:4586357/1‑76 (MQ=255)

                               gTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTaa                                       >  2:5545455/1‑76 (MQ=255)

                               gTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCCaa                                       >  2:6088025/1‑76 (MQ=255)

                               gTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGGCCTGCCAGCACACCCGCTaa                                       >  2:5942627/1‑76 (MQ=255)

                                ttCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAAc                                      >  1:6718984/1‑76 (MQ=255)

                                 tCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACa                                     <  2:3773261/76‑1 (MQ=255)

                                 tCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCCCTAACa                                     >  2:3093915/1‑76 (MQ=255)

                                  cAGCCGGTGCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAg                                    <  1:6612896/76‑1 (MQ=255)

                                  cAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAAcca                                    >  2:804330/1‑74 (MQ=255)

                                  cAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAg                                    >  1:1716951/1‑76 (MQ=255)

                                  cAGCCAGTCCCGGGGCAAGCTGGGTATCGGCAAAAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAg                                    <  2:4185693/76‑1 (MQ=255)

                                    gCCAGTTCCGGGGAAACTTGGGTATCGGCAACAAAGCAGGGAGTGAATGATCCTGCCAGCCCACCCGCTAACAGat                                  <  2:1226444/76‑1 (MQ=255)

                                     ccAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGata                                 >  1:2661213/1‑76 (MQ=255)

                                      cAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGatat                                <  1:5010483/76‑1 (MQ=255)

                                          tCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCcca                            >  2:3162408/1‑76 (MQ=255)

                                          tCCGGGGCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGGCCTGGCAACACACCCGCTAACAGATATCcca                            >  2:2841878/1‑76 (MQ=255)

                                          tCCGGGGCAAGCTTGGTATCGCCAACAAAGCACGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATCcca                            <  1:2110961/76‑1 (MQ=255)

                                           cgggggCAGACTTGTTATCGGCAAGAAAGCACGGAGTGAATGGTCCTGCCAGCCCACCCGCTAACAGATATCccac                           <  1:931329/74‑1 (MQ=255)

                                           ccGGGGCAAGCTTGGGATCGCCAAAAAAGCACGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATCccac                           <  1:4247829/76‑1 (MQ=255)

                                            gggggCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCccacc                          <  1:5444188/75‑1 (MQ=255)

                                            gggggAAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCccacc                          <  1:283349/75‑1 (MQ=255)

                                              gggCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCag                        >  2:5100175/1‑76 (MQ=255)

                                              gggAAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCag                        <  2:912381/76‑1 (MQ=255)

                                               ggCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCaga                       <  1:3264724/76‑1 (MQ=255)

                                                gCAAGCTTGTTATCGTCAAAAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCagag                      <  1:1561625/76‑1 (MQ=255)

                                                gCAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCcgag                      >  2:4604872/1‑76 (MQ=255)

                                                 cAAGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAgagg                     >  2:1337675/1‑76 (MQ=255)

                                                  aaGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAgagga                    >  2:2161275/1‑76 (MQ=255)

                                                   aGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAgaggag                   <  1:5658692/76‑1 (MQ=255)

                                                   aGCTTGGTATCGGCAACAAAGCACGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAgaggag                   <  2:6518351/76‑1 (MQ=255)

                                                     gttGGTATCGGCAACAAAGCACGTAGTTAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaa                 <  2:6860853/75‑1 (MQ=255)

                                                      ttGGTATCGGCAACAAAGCAGGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaaa                <  1:3243919/76‑1 (MQ=255)

                                                       tGGTATCGACAACAAAGCACGGAGGGAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAAt               <  2:5357516/76‑1 (MQ=255)

                                                       gggTATCGGCAACAAAGCCCGGAGTTAACGGACCTGCCAGCTCACCCGCTAACAGATAGCCCACCAGAGGAGAAAt               <  1:4771661/75‑1 (MQ=255)

                                                       gggTATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAAt               <  1:357447/75‑1 (MQ=255)

                                                        ggTAGCGGCAACAAAGCACGGAGTGAATGTTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATa              <  2:1494225/76‑1 (MQ=255)

                                                         ttATCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATAc             <  1:4809838/75‑1 (MQ=255)

                                                         gTATCGGCAAAAAAGCACGTAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATAc             <  1:6890520/76‑1 (MQ=255)

                                                          tctCGCCAACAAACCAGGGAGAGAATGATCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACg            <  2:4432494/74‑1 (MQ=255)

                                                           aTCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGt           <  1:1025773/76‑1 (MQ=255)

                                                           aTCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATCCGt           >  2:4868293/1‑76 (MQ=255)

                                                           aTCGGCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGt           <  2:581616/76‑1 (MQ=255)

                                                              ggCAACAAAGCACGGAGGGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGt        <  1:3379096/76‑1 (MQ=255)

                                                               gCAACAAAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGtt       <  1:1976730/76‑1 (MQ=255)

                                                                caacaaAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGttt      >  2:5625568/1‑76 (MQ=255)

                                                                caacaaAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGttt      <  2:1546682/76‑1 (MQ=255)

                                                                caacaaAGCACGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGttt      <  2:3045111/76‑1 (MQ=255)

                                                                caaaaaAGCACGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGttt      <  2:3611263/76‑1 (MQ=255)

                                                                   caaCGCACGGGGGGAATGGCCCTGCGAGCACGCCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTAtt   <  2:4801387/76‑1 (MQ=255)

                                                                    aaaGCACGGAGTGAATGGCCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTg  <  1:63976/76‑1 (MQ=255)

CCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTG  >  REL606/475088‑475231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`