| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,193,779 | 0 | G | T | 99.5% | 717.6 / NA | 181 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (67/113); minor base A (1/0); total (68/113) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 207 total aligned reads displayed.
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851 | gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATcc < 2:6670565/76‑1 (MQ=255) ccGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATccc < 2:3350401/76‑1 (MQ=255) ccGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGGTGGCGGGAAACAGCCCCCAAAACACGCAAAA‑GCCAAAccc > 1:1831573/1‑76 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTGGGGAAATAGCCCCCATAAAC‑GCAAAACCCCCAAcccc > 1:859530/1‑76 (MQ=255) gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCcca < 1:265197/76‑1 (MQ=255) aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCccac < 1:3054836/76‑1 (MQ=255) aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCccac < 2:6817718/76‑1 (MQ=255) aTGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCCGGAAACAACCCCCAAAACA‑ACAAAACGCCAATcccccc > 2:2878692/1‑74 (MQ=255) gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGGAAACAGCCCCCATAACA‑GCAAAAAGCCAAACCccacca > 1:1321061/1‑76 (MQ=255) gCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCGGGAATCAACCCCAATAACA‑AACAAACACCAAACCccacca > 2:150954/1‑76 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAATCAGCCCCAATAACA‑GCAAAAACCCAAACCCCACCAGa > 1:1073398/1‑76 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAGCCCCAATAAAA‑GCCAAACGCCAAACACCACCAGa > 1:504023/1‑76 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAAAAACCCCCATAAAA‑GCAAAACGCCCAACcccccccgc > 2:600211/1‑73 (MQ=255) tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGAt < 2:2696075/76‑1 (MQ=255) tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGAt < 1:3764626/76‑1 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATa < 2:6558312/76‑1 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATa < 1:1514302/76‑1 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATa < 1:3230235/76‑1 (MQ=255) ccGGCGTCCAGGCGCGGCTGGGCTGGCTGTTGGGAAACAACCCCAATAACA‑GCAAAAAGCCCATCCCCCCCCGATa > 2:4954602/1‑76 (MQ=255) ccGGCGTCCAGGCGCGGCTGGGCTGGCTGGCGGGAATCAGCCCCCATAACA‑GCAAAACGCCAAACCCCCCCAGATa > 1:3296833/1‑76 (MQ=255) cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATaa < 1:3601420/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATaaa < 1:6070942/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATaaa < 1:4360161/76‑1 (MQ=255) cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGc < 2:6624973/76‑1 (MQ=255) cGTCCAGGCGCGGCTGGGCTGGGTGGCGGGAAAAAGCCCCCATAACA‑GCCAAACCCCAAACCCCCCCAGATaaaaa > 2:2601862/1‑74 (MQ=255) cGTCCAGGCGCGGCTGGGCTGGCTGCCCGGAAACAGCGCCAATAACA‑ACAAAAAGCCACACCCCACCAGGTaaaag > 2:5825957/1‑74 (MQ=255) gTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAGCCCCAAAAAAA‑ACCAAAAGCCAAACCCCCCCCAATaaaaac > 2:2145594/1‑73 (MQ=255) gTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAAACGCCCCCAAAACA‑GCAAAAAGCCAAACCCCACCCAATAAAACa > 2:6084052/1‑76 (MQ=255) gTCCAGGCGCGGCTGGGCTGGCTGTCGGAAAACAGCCCCCATAACA‑GCAAAAAGCCCATCCCCACCAGAaaaaaaa > 2:1717969/1‑73 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCAt < 2:5487069/76‑1 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCAt < 2:6332210/76‑1 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCAt < 1:3939726/76‑1 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCAt < 1:6642724/76‑1 (MQ=255) cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGa < 1:2828118/76‑1 (MQ=255) cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGa < 2:2272659/76‑1 (MQ=255) gcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 1:6128830/76‑1 (MQ=255) cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg < 2:2954768/76‑1 (MQ=255) cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg < 2:3654027/76‑1 (MQ=255) cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg < 2:5547435/76‑1 (MQ=255) cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg < 2:1000614/76‑1 (MQ=255) cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg < 2:769289/76‑1 (MQ=255) ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt < 1:117091/76‑1 (MQ=255) ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt < 1:6152344/76‑1 (MQ=255) gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa > 2:6209245/1‑76 (MQ=255) gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa < 2:5359756/76‑1 (MQ=255) cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt < 2:5749220/76‑1 (MQ=255) cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt > 2:1962537/1‑76 (MQ=255) tGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt > 2:781645/1‑76 (MQ=255) tGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt > 2:6888824/1‑76 (MQ=255) ggTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa > 2:4275011/1‑76 (MQ=255) ggTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa < 1:1939053/76‑1 (MQ=255) gTCTNGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa > 2:6138291/1‑76 (MQ=255) gTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa > 1:1341300/1‑76 (MQ=255) cTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc > 1:1279398/1‑76 (MQ=255) tGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa < 2:1662373/76‑1 (MQ=255) tGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa > 2:5918637/1‑76 (MQ=255) gTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt > 1:6191327/1‑76 (MQ=255) tCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt < 1:3138810/76‑1 (MQ=255) cGGAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt < 2:2497959/76‑1 (MQ=255) ggAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:649661/76‑1 (MQ=255) ggAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:5632767/76‑1 (MQ=255) ggAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:1772210/76‑1 (MQ=255) ggAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt > 2:393693/1‑76 (MQ=255) gAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 2:5254429/76‑1 (MQ=255) gAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 1:835423/76‑1 (MQ=255) gAAATCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 1:423447/76‑1 (MQ=255) aaaTCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc < 1:2552120/76‑1 (MQ=255) aaTCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc > 2:6367653/1‑76 (MQ=255) aTCAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg < 2:2412260/76‑1 (MQ=255) cAGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt < 2:3246781/76‑1 (MQ=255) aGCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg > 1:5565251/1‑76 (MQ=255) gCCCCAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg > 2:5663696/1‑76 (MQ=255) ccccAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc < 1:4107237/76‑1 (MQ=255) cccAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg < 1:5156022/76‑1 (MQ=255) cccAATAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg < 2:4613/76‑1 (MQ=255) aTAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTACACAGGCTTTTTACCGATGGCGCATc > 1:3356159/1‑76 (MQ=255) tAACA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg > 1:6240840/1‑76 (MQ=255) aaCA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 1:2378368/1‑76 (MQ=255) aaCA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 1:1410915/1‑76 (MQ=255) cA‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc > 1:2091824/1‑76 (MQ=255) a‑GCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa < 1:6576715/76‑1 (MQ=255) cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc > 2:5876251/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa > 1:1672848/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:1138531/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 1:1546914/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa > 1:5900271/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:3575111/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa > 1:5335276/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa > 1:2865794/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa > 2:3193596/1‑76 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa > 2:4805767/1‑76 (MQ=255) aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcccg > 1:4606091/1‑74 (MQ=255) aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg < 2:3028973/76‑1 (MQ=255) aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc < 1:429302/76‑1 (MQ=255) aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc > 2:1652618/1‑76 (MQ=255) aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc < 2:4163938/76‑1 (MQ=255) accccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc < 2:3774495/73‑1 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 2:6659227/1‑76 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 1:6454942/1‑76 (MQ=255) cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca > 2:1903593/1‑76 (MQ=255) | GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACA‑GCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |