BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,213,517	C→T	A13T (GCT→ACT)	fruK ←	1‑phosphofructokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,213,517	0	C	T	100.0%	794.7 / NA	193	A13T (GCT→ACT)	fruK	1‑phosphofructokinase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (106/87); total (106/87)

Only 100 of 196 total aligned reads displayed.

TGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCG  >  REL606/2213444‑2213589

tGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCg                                                                        <  2:6515732/76‑1 (MQ=255)

tGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCg                                                                        >  1:6843879/1‑76 (MQ=255)

tGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCg                                                                        >  1:5448106/1‑76 (MQ=255)

tGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCg                                                                        >  1:4340416/1‑76 (MQ=255)

 gCAGACCGGTTGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCCGGCAGAAACCAACAAGGTCATAAGTCgg                                                                       <  2:6843364/76‑1 (MQ=255)

  cAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGa                                                                      >  2:2617581/1‑76 (MQ=255)

   aGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGAt                                                                     >  1:190745/1‑76 (MQ=255)

    gACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGAtt                                                                    <  2:1345333/76‑1 (MQ=255)

       cGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAg                                                                 <  2:5045529/76‑1 (MQ=255)

        ggtggtTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAgg                                                                >  1:706810/1‑76 (MQ=255)

        ggcggtTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAgg                                                                <  2:310738/73‑1 (MQ=255)

          tggtTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGt                                                              >  2:4705735/1‑76 (MQ=255)

          tggtTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGt                                                              >  1:951240/1‑76 (MQ=255)

          tggtTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGt                                                              <  1:4059158/76‑1 (MQ=255)

           ggtTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTg                                                             <  1:3802539/76‑1 (MQ=255)

             ttttCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGAt                                                           <  1:1154611/76‑1 (MQ=255)

              tttCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGAta                                                          <  1:3959835/76‑1 (MQ=255)

               ttCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGAtag                                                         >  1:6602650/1‑76 (MQ=255)

               ttCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGAtag                                                         <  1:312960/76‑1 (MQ=255)

                tCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGAtagt                                                        <  1:3210093/76‑1 (MQ=255)

                tCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGAtagt                                                        <  1:6688209/76‑1 (MQ=255)

                tCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGAtagt                                                        <  1:1328604/76‑1 (MQ=255)

                tCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGAtagt                                                        <  1:1552953/76‑1 (MQ=255)

                tCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGAtagt                                                        >  1:4477467/1‑76 (MQ=255)

                   ccAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGc                                                     <  1:2102429/76‑1 (MQ=255)

                    cAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCa                                                    <  1:5744568/76‑1 (MQ=255)

                     aGGTTCACGTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCaa                                                   <  2:1512688/76‑1 (MQ=255)

                       gTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAaca                                                 >  2:3567190/1‑76 (MQ=255)

                       gTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAaca                                                 <  2:5798720/76‑1 (MQ=255)

                        ttCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAacac                                                >  2:4176672/1‑76 (MQ=255)

                         tCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACg                                               >  2:3152441/1‑76 (MQ=255)

                         tCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACg                                               >  2:227879/1‑76 (MQ=255)

                         tCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACg                                               <  1:3715649/76‑1 (MQ=255)

                         tCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACg                                               >  2:1202309/1‑76 (MQ=255)

                          cACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGt                                              <  1:291720/76‑1 (MQ=255)

                          cACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGt                                              <  1:208181/76‑1 (MQ=255)

                          cACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGt                                              <  2:1941553/76‑1 (MQ=255)

                            cTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCt                                            >  2:5585027/1‑76 (MQ=255)

                             ttCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTg                                           >  1:6068001/1‑76 (MQ=255)

                              tCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGc                                          >  1:4958726/1‑76 (MQ=255)

                              tCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGc                                          >  2:3263247/1‑76 (MQ=255)

                                gccgcGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTc                                        <  2:2117512/76‑1 (MQ=255)

                                 ccgcGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCa                                       >  2:4306213/1‑76 (MQ=255)

                                  cgcgTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCAt                                      <  2:159761/76‑1 (MQ=255)

                                     gTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTAt                                   >  1:2835833/1‑76 (MQ=255)

                                     gTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTAt                                   >  1:3621820/1‑76 (MQ=255)

                                     gTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTAt                                   >  1:3777448/1‑76 (MQ=255)

                                      ttCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATg                                  <  1:1806687/76‑1 (MQ=255)

                                      ttCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATg                                  >  2:1751981/1‑76 (MQ=255)

                                      ttCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATg                                  <  2:6555667/76‑1 (MQ=255)

                                       tCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATgc                                 <  2:3288584/76‑1 (MQ=255)

                                       tCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATgc                                 <  2:1911825/76‑1 (MQ=255)

                                       tCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATgc                                 >  2:2974955/1‑76 (MQ=255)

                                       tCAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATgc                                 >  1:3356820/1‑76 (MQ=255)

                                        cAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATgcg                                <  1:481745/76‑1 (MQ=255)

                                        cAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATgcg                                >  2:50251/1‑76 (MQ=255)

                                        cAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATgcg                                >  2:6696964/1‑76 (MQ=255)

                                        cAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATgcg                                <  2:4109105/76‑1 (MQ=255)

                                        cAATTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATgcg                                >  2:2591995/1‑76 (MQ=255)

                                         aaTTTCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATgcgc                               >  1:6436973/1‑76 (MQ=255)

                                            ttCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCt                            >  1:574500/1‑76 (MQ=255)

                                             tCCGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTc                           >  2:2683334/1‑76 (MQ=255)

                                             tCCGGGCAGAAACCAACAAGGTCATAAGTCGGATCAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTc                           <  1:4200387/76‑1 (MQ=255)

                                              ccGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTcc                          >  1:5984138/1‑76 (MQ=255)

                                               cGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTccc                         >  2:4729320/1‑76 (MQ=255)

                                               cGGGCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTccc                         <  1:6243319/76‑1 (MQ=255)

                                                 ggCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCa                       <  2:5353504/76‑1 (MQ=255)

                                                 ggCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCa                       <  2:4823261/76‑1 (MQ=255)

                                                 ggCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCa                       <  1:584937/76‑1 (MQ=255)

                                                  gCAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCaa                      >  2:4433178/1‑76 (MQ=255)

                                                   cAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAg                     >  1:3040732/1‑76 (MQ=255)

                                                   cAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAg                     >  1:2502138/1‑76 (MQ=255)

                                                   cAGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAg                     >  2:6647566/1‑76 (MQ=255)

                                                    aGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGa                    <  2:2395401/76‑1 (MQ=255)

                                                    aGAAACCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGa                    >  2:6066799/1‑76 (MQ=255)

                                                      aaaCCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGAcc                  <  1:2619029/76‑1 (MQ=255)

                                                       aaCCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACca                 <  2:3503988/76‑1 (MQ=255)

                                                       aaCCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACca                 <  2:4501443/76‑1 (MQ=255)

                                                        aCCAACAAGGTCATAAGTCGGATTAATGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACcag                <  1:2744921/76‑1 (MQ=255)

                                                        aCCAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACcag                <  2:800259/76‑1 (MQ=255)

                                                         ccAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACcagc               <  1:4064671/76‑1 (MQ=255)

                                                         ccAACAAGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACcagc               >  2:4278381/1‑76 (MQ=255)

                                                           aacaaGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACcagcag             >  1:1660642/1‑76 (MQ=255)

                                                            acaaGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCagcagc            <  2:6364722/76‑1 (MQ=255)

                                                               aGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGAt         >  1:2316701/1‑76 (MQ=255)

                                                               aGGTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGAt         >  1:1577180/1‑76 (MQ=255)

                                                                ggTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATa        <  1:2230918/76‑1 (MQ=255)

                                                                ggTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATa        <  2:6230099/76‑1 (MQ=255)

                                                                ggTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATa        <  1:4754375/76‑1 (MQ=255)

                                                                 gTCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGCCCAGCAGCGATAg       <  2:3811362/76‑1 (MQ=255)

                                                                  tCATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGc      >  2:5681646/1‑76 (MQ=255)

                                                                   cATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCg     >  2:2193343/1‑76 (MQ=255)

                                                                   cATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCg     >  1:6109244/1‑76 (MQ=255)

                                                                   cATAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCg     >  1:6860081/1‑76 (MQ=255)

                                                                     tAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTc   >  2:2438056/1‑76 (MQ=255)

                                                                     tAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTc   <  1:126292/76‑1 (MQ=255)

                                                                     tAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTc   >  1:4008169/1‑76 (MQ=255)

                                                                     tAAGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTc   >  1:105095/1‑76 (MQ=255)

                                                                      aaGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCg  <  2:5249670/76‑1 (MQ=255)

                                                                      aaGTCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCg  <  2:4185763/76‑1 (MQ=255)

TGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCG  >  REL606/2213444‑2213589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`