| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,875,625 | 1 | . | T | 99.2% | 533.3 / ‑2.7 | 127 | intergenic (‑59/+294) | glmU/atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (61/65); total (62/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01 | |||||||||||
Only 100 of 156 total aligned reads displayed.
ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC > REL606/3875550‑3875695 | tAGCATTATTCAACATACGCGTCCTGACTGTAATCTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑t > 2:451334/1‑76 (MQ=255) aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑tt > 1:518744/1‑76 (MQ=255) aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑tt < 1:1987184/76‑1 (MQ=255) gCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑ttt < 1:119676/76‑1 (MQ=255) gCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑ttt < 2:2310390/76‑1 (MQ=255) gCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑ttt < 2:1475447/76‑1 (MQ=255) attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAACACTACA‑‑ttttt > 2:115926/1‑76 (MQ=255) ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑tttttt > 2:1897060/1‑76 (MQ=255) ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑tttttt < 1:2898304/76‑1 (MQ=255) ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑tttttt > 2:483450/1‑76 (MQ=255) tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑ttttttt > 2:2436957/1‑76 (MQ=255) tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGATTCACCTTGAAAAAACTACA‑‑ttttttt > 2:4666696/1‑76 (MQ=255) attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑tttttttt > 1:2662530/1‑75 (MQ=255) attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑tttttttt > 1:4726637/1‑75 (MQ=255) ttCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑ttttttttc > 1:2071472/1‑74 (MQ=255) ttCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑ttttttttc > 2:4755496/1‑74 (MQ=255) tCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑ttttttttca < 1:1900992/76‑4 (MQ=255) tCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑ttttttttca < 1:3041573/76‑4 (MQ=255) aCATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCg > 2:3831637/1‑76 (MQ=255) cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGt > 2:2036644/1‑76 (MQ=255) aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑TTTTTTTCATCGTGa > 1:4313332/1‑76 (MQ=255) tACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGa > 2:5022811/1‑76 (MQ=255) tACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGa < 2:4461013/76‑1 (MQ=255) aCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGaa > 2:4099470/1‑76 (MQ=255) cgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATg > 1:3166830/1‑76 (MQ=255) tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGa > 2:1565980/1‑76 (MQ=255) tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGa > 1:3250883/1‑76 (MQ=255) tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGa > 2:4380532/1‑76 (MQ=255) tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGa > 2:592739/1‑76 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGAc < 2:4060332/76‑1 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGAc > 1:3736398/1‑76 (MQ=255) cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACa < 2:2427403/76‑1 (MQ=255) tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACag > 1:3534373/1‑76 (MQ=255) gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACaga > 1:706423/1‑76 (MQ=255) gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACaga > 1:1954354/1‑76 (MQ=255) aCTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACagag < 2:370264/76‑1 (MQ=255) aCTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACagag < 1:4651071/76‑1 (MQ=255) cTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAgg < 1:2303108/76‑1 (MQ=255) tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGa < 1:5017428/76‑1 (MQ=255) tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGa > 2:3900965/1‑76 (MQ=255) gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGAt < 1:4415549/76‑1 (MQ=255) gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGAt < 2:708465/76‑1 (MQ=255) gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGAt < 1:2832983/76‑1 (MQ=255) gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGAt < 1:2404499/76‑1 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATaa > 2:4902728/1‑76 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATaaa > 1:3218802/1‑76 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATaaa < 2:1859339/76‑1 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATaaa > 1:4063/1‑76 (MQ=255) aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATaaa < 1:84507/76‑1 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAAtt > 1:3670333/1‑76 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAAtt < 2:2112270/76‑1 (MQ=255) tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTg > 1:1344108/1‑76 (MQ=255) agaACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGtt < 1:1806585/76‑1 (MQ=255) agaACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGtt > 2:2891527/1‑76 (MQ=255) gaACGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTc < 1:3939678/76‑1 (MQ=255) aCGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCaa < 1:375947/76‑1 (MQ=255) cGAATTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt < 2:658170/76‑1 (MQ=255) aaTTTAAACCGCTTCATCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa < 1:3371944/76‑1 (MQ=255) aaTTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa < 2:4273099/76‑1 (MQ=255) aaTTTAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa > 2:469321/1‑76 (MQ=255) tttAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg > 1:3704358/1‑76 (MQ=255) tttAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg > 2:3131648/1‑76 (MQ=255) ttAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg > 2:1549560/1‑76 (MQ=255) ttAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg < 2:211722/76‑1 (MQ=255) tAAACCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 2:2817209/1‑76 (MQ=255) aaaCCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt < 1:1528860/76‑1 (MQ=255) aaCCGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 1:3540700/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 2:1883405/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 2:3609882/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 1:3811270/76‑1 (MQ=255) cGCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATc > 1:4257287/1‑76 (MQ=255) gCTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcc < 2:3534812/76‑1 (MQ=255) cTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATccc < 2:3091722/76‑1 (MQ=255) cTTCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATccc > 2:3619403/1‑76 (MQ=255) ttCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc < 2:97323/76‑1 (MQ=255) ttCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc > 2:750659/1‑76 (MQ=255) ttCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc > 2:1136217/1‑76 (MQ=255) tCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccca < 2:3489020/76‑2 (MQ=255) tCACCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg < 1:2828855/76‑1 (MQ=255) aCCTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCt > 1:429618/1‑76 (MQ=255) ccTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg > 2:3285654/1‑76 (MQ=255) ccTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg < 2:3706511/76‑1 (MQ=255) ccTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg < 2:1804665/76‑1 (MQ=255) cTTGAAAAAACTACATTTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg > 1:3329200/1‑76 (MQ=255) cTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa > 1:1122609/1‑76 (MQ=255) cTTGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa > 1:4162029/1‑76 (MQ=255) ttGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc > 1:1313063/1‑76 (MQ=255) tGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg > 1:2560271/1‑76 (MQ=255) tGAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg > 1:207472/1‑76 (MQ=255) gAAAAAGCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGa < 1:702972/76‑1 (MQ=255) gAAAAAACTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGa < 1:1775690/76‑1 (MQ=255) aaaaaaCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCCATAACGGATTATCCCCGCTGACGaa < 1:2726368/76‑1 (MQ=255) aaaaaCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa < 1:5049924/76‑1 (MQ=255) aaaaCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg < 1:5048343/76‑1 (MQ=255) aaaaCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg > 2:4786609/1‑76 (MQ=255) aaaaCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg < 1:1047561/76‑1 (MQ=255) aaaCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc > 2:4839133/1‑76 (MQ=255) aaaCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc < 1:1855553/76‑1 (MQ=255) aaCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcg > 1:1207390/1‑76 (MQ=255) aCTACAT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcgc > 1:4519255/1‑76 (MQ=255) | ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC > REL606/3875550‑3875695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |