BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,911,972	(T)_7→8	intergenic (‑173/‑150)	yifB ← / → ilvL	predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,911,965	1	.	T	100.0%	521.6 / NA	121	intergenic (‑166/‑157)	yifB/ilvL	predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide
Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (40/81); total (40/81)

Only 100 of 138 total aligned reads displayed.

GGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAA  >  REL606/3911890‑3912034

ggAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGaa                                                                        <  1:2230944/76‑1 (MQ=255)

ggAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGaa                                                                        <  2:281640/76‑1 (MQ=255)

ggAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGaa                                                                        <  2:347401/76‑1 (MQ=255)

 gAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑t                                                                      <  1:1145995/76‑1 (MQ=255)

  aaTGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tt                                                                     >  1:3634128/1‑76 (MQ=255)

   atgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt                                                                    >  1:2993719/1‑76 (MQ=255)

    tgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttt                                                                   >  1:2391445/1‑76 (MQ=255)

      atgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttttt                                                                 <  1:4558769/76‑1 (MQ=255)

      atgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttttt                                                                 >  2:2913770/1‑76 (MQ=255)

       tgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttttttt                                                                >  1:1874763/1‑76 (MQ=255)

       tgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttttttt                                                                >  2:3027384/1‑76 (MQ=255)

        gCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttttttt                                                               >  1:2613276/1‑75 (MQ=255)

        gCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttttttt                                                               >  1:4740927/1‑75 (MQ=255)

         cTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttttttttc                                                              <  2:484312/76‑3 (MQ=255)

         cTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttttttttc                                                              >  1:1173094/1‑74 (MQ=255)

          tCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttttttttca                                                             <  2:5039394/76‑4 (MQ=255)

              aaaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactca                                                          <  2:380395/76‑1 (MQ=255)

              aaaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactca                                                          >  1:262851/1‑76 (MQ=255)

              aaaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactca                                                          <  1:3008332/76‑1 (MQ=255)

               aaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactcac                                                         <  2:2648873/76‑1 (MQ=255)

                aaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTcactcact                                                        <  2:2461773/76‑1 (MQ=255)

                 aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta                                                       >  1:4040620/1‑76 (MQ=255)

                 aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta                                                       <  2:4456860/76‑1 (MQ=255)

                  aTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtat                                                      <  2:3525369/76‑1 (MQ=255)

                  aTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtat                                                      <  2:3677985/76‑1 (MQ=255)

                  aTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtat                                                      <  2:1505056/76‑1 (MQ=255)

                  aTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtat                                                      <  1:898882/76‑1 (MQ=255)

                  aTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtat                                                      <  1:5103185/76‑1 (MQ=255)

                   tGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAAATGAATTTTTTTTCACTCACtatt                                                     <  1:3003372/76‑1 (MQ=255)

                     cAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatttt                                                   <  2:3849917/76‑1 (MQ=255)

                      aGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatttta                                                  <  2:4612961/76‑1 (MQ=255)

                      aGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatttta                                                  <  1:4794227/76‑1 (MQ=255)

                      aGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatttta                                                  <  2:1306199/76‑1 (MQ=255)

                      aGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatttta                                                  <  1:2204630/76‑1 (MQ=255)

                      aGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatttta                                                  <  2:5129436/76‑1 (MQ=255)

                        cGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattttatt                                                <  1:4247469/76‑1 (MQ=255)

                        cGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattttatt                                                <  1:4928375/76‑1 (MQ=255)

                          gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTattttatttt                                              <  2:3265071/76‑1 (MQ=255)

                          gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTattttatttt                                              <  1:1300961/76‑1 (MQ=255)

                              aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaa                                          >  1:2168455/1‑76 (MQ=255)

                              aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaa                                          >  2:1291045/1‑76 (MQ=255)

                              aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaa                                          <  1:3110715/76‑1 (MQ=255)

                              aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaa                                          <  1:154981/76‑1 (MQ=255)

                               aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaa                                         <  1:407380/76‑1 (MQ=255)

                               aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaa                                         <  2:1215971/76‑1 (MQ=255)

                                 gATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaaaa                                       <  2:2284378/76‑1 (MQ=255)

                                 gATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaaaa                                       <  2:3785075/76‑1 (MQ=255)

                                  aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaac                                      <  1:2127232/76‑1 (MQ=255)

                                    gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaa                                    <  1:3431326/76‑1 (MQ=255)

                                    gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaa                                    <  1:2268358/76‑1 (MQ=255)

                                    gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaa                                    <  1:599502/76‑1 (MQ=255)

                                     aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaac                                   >  2:2619784/1‑76 (MQ=255)

                                      aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAacaaca                                  <  1:259059/76‑1 (MQ=255)

                                       cTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAAcaacaa                                 <  1:3109526/76‑1 (MQ=255)

                                        tACGAGGAAGGGAACAACATTCATGCTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAt                                <  2:2667447/76‑1 (MQ=255)

                                        tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAt                                <  1:3228972/76‑1 (MQ=255)

                                        tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAt                                >  2:3302720/1‑76 (MQ=255)

                                         aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAtt                               <  1:2362484/76‑1 (MQ=255)

                                         aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAtt                               <  2:4300620/76‑1 (MQ=255)

                                          cGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAttt                              >  2:1146403/1‑76 (MQ=255)

                                           gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTta                             <  2:2494798/76‑1 (MQ=255)

                                           gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTta                             >  2:4003534/1‑76 (MQ=255)

                                            aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat                            <  1:5070196/76‑1 (MQ=255)

                                             ggAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtata                           >  1:420926/1‑76 (MQ=255)

                                             ggAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtata                           >  1:3536297/1‑76 (MQ=255)

                                              gAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTatat                          <  1:585732/76‑1 (MQ=255)

                                              gAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTatat                          <  2:760705/76‑1 (MQ=255)

                                               aaGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATAtt                         >  2:3740575/1‑76 (MQ=255)

                                                aGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg                        <  1:1291186/76‑1 (MQ=255)

                                                aGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg                        <  1:1606870/76‑1 (MQ=255)

                                                aGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg                        <  2:765684/76‑1 (MQ=255)

                                                aGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg                        >  1:269173/1‑76 (MQ=255)

                                                 gggAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGa                       <  1:1288752/76‑1 (MQ=255)

                                                 gggAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGa                       <  1:1668982/76‑1 (MQ=255)

                                                  ggAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa                      <  1:3396732/76‑1 (MQ=255)

                                                  ggAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa                      <  1:4650134/76‑1 (MQ=255)

                                                  ggAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa                      <  2:3647377/76‑1 (MQ=255)

                                                   gAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaaa                     <  1:705210/76‑1 (MQ=255)

                                                     acaacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatt                   <  2:1194143/76‑1 (MQ=255)

                                                      caacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatta                  <  1:4796909/76‑1 (MQ=255)

                                                       aacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattat                 <  1:1515469/76‑1 (MQ=255)

                                                       aacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattat                 >  2:357861/1‑76 (MQ=255)

                                                        acaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattatt                <  1:2320123/76‑1 (MQ=255)

                                                         caTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAAttatta               <  1:2780251/76‑1 (MQ=255)

                                                          aTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaa              >  2:4263885/1‑76 (MQ=255)

                                                          aTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaa              <  2:2110264/76‑1 (MQ=255)

                                                           ttCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaaa             <  1:1103897/76‑1 (MQ=255)

                                                            tCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAAc            >  1:1894504/1‑76 (MQ=255)

                                                            tCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAAc            <  1:3997097/76‑1 (MQ=255)

                                                             cATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACg           <  2:2331108/76‑1 (MQ=255)

                                                             cATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACg           >  1:2024147/1‑76 (MQ=255)

                                                             cATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACg           <  2:1942569/76‑1 (MQ=255)

                                                              aTACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGc          <  1:1988343/76‑1 (MQ=255)

                                                               tACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGca         >  1:893242/1‑76 (MQ=255)

                                                                aCTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat        >  1:16517/1‑76 (MQ=255)

                                                                aCTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat        <  2:4950816/76‑1 (MQ=255)

                                                                 cTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatc       >  2:2411052/1‑76 (MQ=255)

                                                                  tGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatca      >  1:1915802/1‑76 (MQ=255)

                                                                  tGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatca      >  1:3880920/1‑76 (MQ=255)

                                                                     taTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa   >  2:3947513/2‑76 (MQ=255)

GGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAA  >  REL606/3911890‑3912034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`