| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,044,357 | 0 | A | C | 35.1% | 301.1 / 69.1 | 148 | V284G (GTG→GGG) | rfbD | dTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase |
| Reads supporting (aligned to +/- strand): ref base A (86/10); new base C (0/52); total (86/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.36e-30 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 166 total aligned reads displayed.
CATCATGAGCGAGATGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTCGA > REL606/2044282‑2044431 | catcatGAGCGAGATGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCAcaccca < 2:1641595/76‑1 (MQ=255) catGAGCGAGATGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCAcacccacca < 2:724875/76‑2 (MQ=255) catGAGCGAGATGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCt < 2:272295/76‑1 (MQ=255) catGAGCGAGATGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCt > 1:2480703/1‑76 (MQ=255) catGAGCGAGATGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCt > 1:2407766/1‑76 (MQ=255) atGAGCGAGATGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTg < 2:1838519/76‑1 (MQ=255) atGAGCGAGATGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTg > 1:2148948/1‑76 (MQ=255) tGAGCGAGATGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGc > 2:1071083/1‑76 (MQ=255) gAGCGAGATGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGcc > 1:4119901/1‑76 (MQ=255) gAGCGAGATGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGcc < 1:216026/76‑1 (MQ=255) gCGAGATGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAg < 2:1370737/76‑1 (MQ=255) cGAGATGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGt > 1:726116/1‑76 (MQ=255) agaTGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCa > 2:1197935/1‑76 (MQ=255) tGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGc > 2:4249719/1‑76 (MQ=255) taaaaaaTTTT‑TTAAATTGCCCGTAGTCGAAAA‑AATTTGTTTGGCCTTTCTTTCCCCCCCCCCCTGCCAGTCAGGc < 2:3175544/74‑1 (MQ=255) taaaaaaTTTGTTTAAATTG‑CCGTAGTCGAAAA‑TAATTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGc < 2:2410759/74‑1 (MQ=255) cAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCaa < 1:2833756/76‑1 (MQ=255) cAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCaa > 2:4141197/1‑76 (MQ=255) cAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCaa > 1:1507792/1‑76 (MQ=255) cAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCaa > 1:3230895/1‑76 (MQ=255) aaatTTTG‑TAAAATGG‑CCGTAGTCGAAAA‑TAATTCGTTGAGCTTTCGTTTCCCCCCCCCCTGCCAGTCAGGCAAGa < 2:3019017/76‑1 (MQ=255) aaaaTTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGa > 1:4190642/1‑76 (MQ=255) aaaTTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGAc > 2:3492967/1‑76 (MQ=255) aaTTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACa > 1:1481914/1‑76 (MQ=255) aTTTT‑TTAAATTG‑CCGTAGTCTAAAA‑TAATTCGTGGAGCATCCGTTTCACACCCCCCTGCCAGTCAGGCAAGACaa < 2:1085421/76‑1 (MQ=255) aTTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACaa > 1:3955518/1‑76 (MQ=255) tttG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAg < 2:2663279/76‑1 (MQ=255) tttG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAg > 1:2335519/1‑76 (MQ=255) tttG‑TTAAATGG‑CCGTAGTCAAAAA‑TAATTCGTTGAGCATTCGTTCCACCCCCCCCTGCCAGTCAGGCAAGACAAg < 1:1459639/76‑1 (MQ=255) tttG‑TAAAATTG‑CCGTAGTGGTAAA‑TATTTCGTTGAGCTTTCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAg < 1:1389543/76‑1 (MQ=255) ttG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAgc > 2:2311837/1‑76 (MQ=255) tG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAgcg > 2:712552/1‑76 (MQ=255) ttttAAGTTG‑CCGTAGTCGGAAA‑TAATCCTTTGGACATTCGTTGCACCCCCCCCTGCCAGTCAGGCAAGACAAgcg < 2:449367/74‑1 (MQ=255) g‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAgcgc > 1:1284105/1‑76 (MQ=255) ttAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCa < 1:1219909/76‑1 (MQ=255) ttAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCa > 1:288194/1‑76 (MQ=255) aatttG‑CCGTAGT‑GTAAATTATTTCTTGGACCATTCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCaaa < 1:4173681/73‑1 (MQ=255) aaaTGG‑CGGTAGTCGAAAA‑TAATTCGTGGAGCTTTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCaaa < 2:343442/76‑1 (MQ=255) aaTTG‑CCGTAGTCGAAAA‑TATTTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAg < 2:2589196/76‑1 (MQ=255) ttgg‑CCGTAGTCGTAAA‑TATTTCGTTGAGCTTTCTTTTCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGt < 2:2823954/73‑1 (MQ=255) aTTG‑CCGTAGTCTAAAA‑TAATTCGTTGAGCATTCGTTCCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGt < 1:1589338/76‑1 (MQ=255) aTTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGt > 1:2857091/1‑76 (MQ=255) aTTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGt > 2:2113243/1‑76 (MQ=255) tG‑CTGTATTCGTAAA‑TAATTGTTTGACCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTc < 2:4647925/76‑1 (MQ=255) tG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTc < 2:423269/76‑1 (MQ=255) g‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTct > 2:2063696/1‑76 (MQ=255) g‑CCGTAGTCGAAAA‑TAATTGTTGGAGCATTCTTTCCACACCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTct < 2:3639078/76‑1 (MQ=255) cccGTAGTCGAAAA‑TAATTCTTTGAGCTTTCTTTCCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTct < 2:4023764/75‑1 (MQ=255) tAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctgctg > 2:1403290/1‑76 (MQ=255) aGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGa > 2:1973466/1‑76 (MQ=255) aGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGa > 1:3841988/1‑76 (MQ=255) aGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGa > 2:291480/1‑76 (MQ=255) gTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGaa > 2:2255594/1‑76 (MQ=255) tCGAAAA‑TATTTCGTGGAGCATTCTTTTCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGaaa < 2:531565/76‑1 (MQ=255) gTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACt > 1:3386730/1‑76 (MQ=255) gTAAA‑TAATTCGTGGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACt < 2:1192195/76‑1 (MQ=255) atAAA‑TAATTCGTTGAGCTTTCTTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACt < 1:298400/75‑1 (MQ=255) tAAA‑TATTTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtt < 2:650679/76‑1 (MQ=255) tAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtt > 2:3199481/1‑76 (MQ=255) tAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtt > 2:3779397/1‑76 (MQ=255) aaa‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACttt > 2:2498/1‑76 (MQ=255) aa‑taatTCGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtttt < 2:1280005/76‑1 (MQ=255) aa‑taatTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtttt > 2:3751360/1‑76 (MQ=255) aa‑taatTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtttt > 1:832013/1‑76 (MQ=255) aa‑taatTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtttt > 1:4792828/1‑76 (MQ=255) a‑tattTCGTGGAGCATCCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTc < 1:4608004/76‑1 (MQ=255) a‑taatTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTc > 2:1045191/1‑76 (MQ=255) atTCGTTGAGCTTTCTTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGt < 1:3884050/76‑1 (MQ=255) atTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGt > 1:12692/1‑76 (MQ=255) tgcGTTGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTa < 1:2541799/74‑1 (MQ=255) tgcGTGGAGCATTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTa < 2:4901470/74‑1 (MQ=255) gtttGACCTTTCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTAtt < 2:2611841/74‑1 (MQ=255) cGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTAtt > 1:4895411/1‑76 (MQ=255) cGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTAtt > 2:3612339/1‑76 (MQ=255) cGTTGACCTTTCGTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTAtt < 2:3538992/76‑1 (MQ=255) gtcgAGCATTCTTTTCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTg < 1:1217110/73‑1 (MQ=255) tGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAg > 1:2589818/1‑76 (MQ=255) tGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAg > 2:4280832/1‑76 (MQ=255) tGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAg > 2:2340364/1‑76 (MQ=255) gAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAgg > 1:5056402/1‑76 (MQ=255) cATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCga > 1:3711494/1‑76 (MQ=255) aTTCGTTCCACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCgag < 1:983174/76‑1 (MQ=255) ttCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCgaga > 2:1507294/1‑76 (MQ=255) ttCGTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCgaga < 1:1710827/76‑1 (MQ=255) tCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGagag > 1:3280319/1‑76 (MQ=255) tttCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTa > 2:4794290/1‑76 (MQ=255) tttCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTa > 2:2284294/1‑76 (MQ=255) ttCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTAt > 1:2031180/1‑76 (MQ=255) ttCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTAt > 1:4428624/1‑76 (MQ=255) tccaccCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTAt < 1:4661262/74‑1 (MQ=255) tCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATg > 2:4725752/1‑76 (MQ=255) tCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATg > 2:2193655/1‑76 (MQ=255) tCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATg > 1:4053653/1‑76 (MQ=255) tCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATg > 2:84448/1‑76 (MQ=255) cacaCCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATgg > 1:2767164/1‑76 (MQ=255) ccccccccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATgggg < 2:493853/74‑1 (MQ=255) ccccccccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATgggg < 2:5030358/74‑1 (MQ=255) cacccaccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATgggg > 2:1950144/1‑76 (MQ=255) cccccccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGt < 2:1634458/75‑1 (MQ=255) caccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTCGa > 1:4982790/1‑76 (MQ=255) | CATCATGAGCGAGATGCAAAAATTTG‑TTAAATTG‑CCGTAGTCGTAAA‑TAATTCGTTGAGCATTCGTTTCACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTCGA > REL606/2044282‑2044431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |