| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,378,885 | 0 | T | G | 37.0% | 290.3 / 30.2 | 138 | T129P (ACC→CCC) | rplB | 50S ribosomal protein L2 |
| Reads supporting (aligned to +/- strand): ref base T (14/73); new base G (51/0); total (65/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-25 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 176 total aligned reads displayed.
GCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAT > REL606/3378810‑3378960 | gCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgt < 2:406712/76‑1 (MQ=255) cTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgtg < 2:2126223/76‑1 (MQ=255) tGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgtgt > 2:2355201/1‑76 (MQ=255) gACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGCCAGGGTGtt > 2:1937613/1‑76 (MQ=255) aCCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTa < 2:1752147/76‑1 (MQ=255) ccGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTAc < 2:879993/76‑1 (MQ=255) ccGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTAc < 2:4617366/76‑1 (MQ=255) ccGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTAc < 1:2377159/76‑1 (MQ=255) cGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTAcc < 1:3047679/76‑1 (MQ=255) cGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTAcc > 2:1625018/1‑76 (MQ=255) cGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTAcc > 1:3648003/1‑76 (MQ=255) gCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCt < 1:957286/76‑1 (MQ=255) ccTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTg > 2:1390087/1‑76 (MQ=255) ccTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTAACTg > 1:4523715/1‑76 (MQ=255) ccTTTACCTGGTTTCATTGCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTg < 2:3870075/76‑1 (MQ=255) cTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTgg < 1:493509/76‑1 (MQ=255) cTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTgg > 1:3806561/1‑76 (MQ=255) cTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTgg > 2:265111/1‑76 (MQ=255) cTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTTCCTgg > 1:4592626/1‑76 (MQ=255) cTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTgg > 1:3190543/1‑76 (MQ=255) tttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGt < 1:2865165/76‑1 (MQ=255) tttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGt > 1:1430886/1‑76 (MQ=255) ttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTgggt > 1:3483209/1‑74 (MQ=255) ttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGtt > 2:1261825/1‑76 (MQ=255) aCCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTg < 1:4041436/76‑1 (MQ=255) aCCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTg < 2:4822439/76‑1 (MQ=255) aCCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTg < 1:2977076/76‑1 (MQ=255) aCCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTTGTTTg > 2:3991270/1‑76 (MQ=255) ccTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGGTTGa > 2:4330081/1‑76 (MQ=255) tGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGAtt < 2:5089017/76‑1 (MQ=255) tGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTTCCTGGTTTGAtt > 2:4008581/1‑76 (MQ=255) tttCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTTGTTTTATTgca > 1:4874511/1‑76 (MQ=255) ttCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTTATTgcag > 1:3794765/1‑76 (MQ=255) ttCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTtcag > 2:4305335/1‑76 (MQ=255) ttCACTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgcag < 2:4485938/76‑1 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTATCTGGGTTGATTgaagca > 2:4252120/1‑76 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTgcagca < 1:2808825/76‑1 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGTTTGATTgcagca > 2:3363777/1‑76 (MQ=255) tttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATc > 2:1000780/1‑76 (MQ=255) tttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTTATTTCAGCATc > 2:2861386/1‑76 (MQ=255) tCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACCTGGGTTGATTGCAGCATTaa > 1:2957115/1‑76 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGGTACCTGGTTTGATTGCAGCATCAAc > 1:5104117/1‑76 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTTCCTGGTTTGATTGTAGCATCaaa > 1:3409542/1‑75 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAAc > 2:1530357/1‑76 (MQ=255) tACGTTACGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGCAGCATCAACg > 2:4497363/1‑76 (MQ=255) cGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGGAGCATCAACGcc > 1:1823127/1‑76 (MQ=255) cGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGCAGCATCCACGcc > 2:9073/1‑76 (MQ=255) gTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGGTACCTGGTTTTATTGCAGCATCAAAGCCa > 1:1101731/1‑76 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGcccg > 2:2536699/1‑74 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAg > 2:1953019/1‑76 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGCAGCATCAACGCCAg > 2:1129680/1‑76 (MQ=255) tGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACt < 2:1665343/76‑1 (MQ=255) tGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACt > 1:2383880/1‑76 (MQ=255) aaCAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGa > 2:2041236/1‑76 (MQ=255) cAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTTCCTGGTTTGATTGCAGCATCAACGCCAGACTGAAt > 2:3713972/1‑76 (MQ=255) gTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCt < 1:2127910/76‑1 (MQ=255) tAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGGTTGATTGCAGCATCAACGCCAGACTGAATCTg > 2:2582663/1‑76 (MQ=255) tAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAAACTg > 2:4017649/1‑76 (MQ=255) tAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTGGGTTGATTGCAGAATCAACGCCAGAATGAATCTg > 2:519110/1‑76 (MQ=255) aaccaaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTc < 1:3296243/76‑1 (MQ=255) ccaaccGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGc > 1:1950269/1‑76 (MQ=255) caaccGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCCGACTGAATCTGGTCGcc > 1:3387365/1‑76 (MQ=255) caaccGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGcc > 1:2820589/1‑76 (MQ=255) aaccGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCa < 2:4963646/76‑1 (MQ=255) aaccGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGGCGCCa > 1:4760598/1‑76 (MQ=255) ccGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGc > 1:4825721/1‑76 (MQ=255) cGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCt < 2:4552594/76‑1 (MQ=255) cGGGATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCt > 1:198233/1‑76 (MQ=255) gggATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCtt < 2:604535/76‑1 (MQ=255) ggATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATGGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCttt < 2:3271877/76‑1 (MQ=255) gATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTc < 1:2342788/76‑1 (MQ=255) gATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTc < 1:3225903/76‑1 (MQ=255) gATGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTGGCAAGCTTTc > 2:3063007/1‑76 (MQ=255) aTGTTGCGCATCGGCAGGGGGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCa > 1:220895/1‑76 (MQ=255) tGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAg > 1:700989/1‑76 (MQ=255) tGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGcc < 1:1511154/76‑1 (MQ=255) gcgcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCt < 1:1598972/76‑1 (MQ=255) cgcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCtt < 2:4670113/76‑1 (MQ=255) gcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCttt < 1:882236/76‑1 (MQ=255) tCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAgg < 1:3748879/76‑1 (MQ=255) cGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAggg < 2:2222626/76‑1 (MQ=255) ggCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAgggg < 2:1354507/76‑1 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc < 1:1055777/76‑1 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc > 2:1788029/1‑76 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc < 1:3420149/76‑1 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc > 2:3528685/1‑76 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc > 2:3557196/1‑76 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc < 1:2488001/76‑1 (MQ=255) cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGNCNCCAGCTTTCAGGCCTTTAGGGGcc < 1:613901/76‑1 (MQ=255) aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCa > 2:4215366/1‑76 (MQ=255) aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCa > 2:1536958/1‑76 (MQ=255) aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCa < 2:1456399/76‑1 (MQ=255) ggTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAgg < 1:3401152/76‑1 (MQ=255) ggTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAgg < 1:5005305/76‑1 (MQ=255) ggTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAgg > 1:2715081/1‑76 (MQ=255) tgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt > 1:3474305/1‑76 (MQ=255) tgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt < 1:4343211/76‑1 (MQ=255) tgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt < 2:4945022/76‑1 (MQ=255) tgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt > 1:1251003/1‑76 (MQ=255) tgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt > 1:574016/1‑76 (MQ=255) | GCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAT > REL606/3378810‑3378960 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |