| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,392,556 | 0 | C | T | 41.9% | 96.6 / 73.4 | 105 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (45/16); new base T (0/44); total (45/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.43e-16 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 154 total aligned reads displayed.
CAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTG > REL606/4392483‑4392631 | cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGCTTTTCTa > 1:5005285/1‑76 (MQ=255)cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa < 2:5123936/76‑1 (MQ=255) aaCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAg > 2:4053793/1‑76 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTAGcc > 1:4891040/1‑76 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGGGTTTCTAggc > 1:3738996/1‑74 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCa > 2:3275928/1‑76 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAg < 1:2157810/76‑1 (MQ=255) ataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGt > 2:4697034/1‑76 (MQ=255) taCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTa > 2:3244424/1‑76 (MQ=255) cccTGTCTGAGGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCGGGGATAGCGTTTTTTTAGCCAGTAt < 2:4804037/75‑1 (MQ=255) aCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTAt > 1:3785420/1‑76 (MQ=255) ccggTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTAtt < 2:645021/73‑1 (MQ=255) ccTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGAAGCGGATTTCTCGCCAGTGtt > 2:1960174/1‑76 (MQ=255) cTGTCTGGGGGCCGGCTCGGCGAGGCGGTAACGCGTGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTg < 2:1403713/76‑1 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGCTTTTCTAGCCAGTATTg > 1:1215873/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCCGTATTg > 1:1626641/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTg > 2:2256657/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGGAttt > 1:4502198/1‑75 (MQ=255) tGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTgg > 1:4019158/1‑76 (MQ=255) tGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGGATTgg > 1:768547/1‑76 (MQ=255) tCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTTGCACGTATTTGTc > 1:3506180/1‑76 (MQ=255) tCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCCGTATTGGTc > 1:4231270/1‑76 (MQ=255) tCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGCAGCCAGTTTCCAGCCAGCTTTGGTc > 2:4903295/1‑76 (MQ=255) cggATGGCGCGCTCGGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTca < 2:2051508/74‑1 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTTGTca > 1:3660694/1‑76 (MQ=255) tGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTAGCCAGGATTGGTcag > 1:8717/1‑76 (MQ=255) tGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTcag > 1:163985/1‑76 (MQ=255) gggtGGCGCGCTCTGGGAAGCGGTAACGGGTGAAGGTGCGGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTcag < 2:516543/73‑1 (MQ=255) gATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGGcagc > 1:1893365/1‑76 (MQ=255) tGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTAGCCAGTATTGGTcagcag > 1:244080/1‑76 (MQ=255) tGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTcagcag > 1:2209732/1‑76 (MQ=255) gcggcTCTGGGAGGCGGTAGCGCGAAAAGGGGCTGGCTGGGAGAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTa < 1:3586772/73‑1 (MQ=255) cgcgcTCTGCGAGGCGGTAACGCGGGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTa < 2:332246/76‑1 (MQ=255) cgcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTa > 1:3408808/1‑76 (MQ=255) cgcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTa > 2:3005755/1‑76 (MQ=255) cgcgcTCGGCGAAGCGGTACGGCGTGAAGGTGCTGGCTGGGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTa < 1:3449620/76‑1 (MQ=255) cccgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTa < 1:4675277/74‑1 (MQ=255) gcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAg < 1:4488269/76‑1 (MQ=255) gcgcTCGGCGAAGGGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAg < 1:2901294/76‑1 (MQ=255) cgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAgg < 2:3562692/76‑1 (MQ=255) gcTCTGCAAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGa < 1:4120815/76‑1 (MQ=255) ctctGCGAAGCGGTAACGCTTGAGGGTGCTGGCGGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGaa < 1:3431648/76‑1 (MQ=255) ctctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGaa < 2:2347227/76‑1 (MQ=255) ctctGCGAAGCGGTAACGCGTGAAGGGGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGaa < 1:2147367/76‑1 (MQ=255) cgctGCAAAGCGGTAACGCGTGAAGGTGCTGGCGGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGaa < 1:4219880/74‑1 (MQ=255) tctGCGAGGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAAc < 2:3527660/76‑1 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACg > 1:3806226/1‑76 (MQ=255) gCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGGGATTTTCTAATCAGTATTTGGCAGCAGATAAGGACGG‑c > 2:500331/1‑76 (MQ=255) ggAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑Ct < 1:726359/75‑1 (MQ=255) cGAGGCGGTAACGGGTGAAGGTGCTGGTGGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑Ct < 1:598703/76‑1 (MQ=255) cGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑cg > 2:4180772/1‑75 (MQ=255) cGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACgggt > 1:50295/1‑74 (MQ=255) cGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑Ct > 1:4927170/1‑76 (MQ=255) gCGTTAACGCTTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAAt < 2:4346551/76‑1 (MQ=255) gCGGTAACGGGTGAGGGTGCTGGCTGGGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAAt < 1:1074378/76‑1 (MQ=255) gggTAACGCGTGAAGGTGCTGGTGGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATc < 1:4369804/75‑1 (MQ=255) cGGTAGCGCGTGAGGGTTCTGGCTGGGATAGCTTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATc < 1:279682/76‑1 (MQ=255) cGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGaaac > 1:3055478/1‑74 (MQ=255) ggTAACGGGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATcc < 1:2068888/76‑1 (MQ=255) ggTAACGCGTGAAGGTGCTGGCGGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATcc < 1:150720/76‑1 (MQ=255) ggTAACGCGGGAGGGTGCTGGCGGGGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATcc < 2:4322832/76‑1 (MQ=255) aaCGCGTGAGGGTGCGGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCtt < 1:1175102/76‑1 (MQ=255) aaCGCGTGAAGGTGCTGGCTGGGATAGTGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCtt < 1:796302/76‑1 (MQ=255) cgcgTGAAGGTGCTGGCGGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGa < 1:4979263/76‑1 (MQ=255) gcgTGAAGGTGCTGGCTTGGATAGGGATTTTCTAAACCGTATTTGTTAGCAGTTAGGGACGGCCGGAATCCCTTGa > 2:3992753/1‑76 (MQ=255) gTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACt < 1:3651706/76‑1 (MQ=255) gTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACt < 1:4716093/76‑1 (MQ=255) tGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTg < 1:5126752/76‑1 (MQ=255) aaGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTggg < 2:615216/76‑1 (MQ=255) aaGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGGATTGGTCAGCAGTTAGGAACGG‑GTGCATTCCTTGAACTtgg > 1:1321596/1‑76 (MQ=255) aGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTgggg < 1:4180252/76‑1 (MQ=255) ttGCGGGGTGGAATATCAATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGggcg < 1:3767819/75‑1 (MQ=255) tGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGggcgg > 1:3227504/1‑76 (MQ=255) tctggcgggGATAGTTTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGggcggc < 2:4903649/75‑1 (MQ=255) ctggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGggcgggt > 2:2967632/1‑74 (MQ=255) tggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGggcggccg > 2:1117663/1‑74 (MQ=255) gctggGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGc < 2:1969032/76‑1 (MQ=255) gctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTggg > 2:3353916/1‑75 (MQ=255) ctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTgggg > 1:325900/1‑74 (MQ=255) tggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGGGa > 1:1005428/1‑76 (MQ=255) tggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGa > 2:2959729/1‑76 (MQ=255) ggtaTAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGaa < 2:4976755/73‑1 (MQ=255) ggATAGTGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAAt < 1:3487159/76‑1 (MQ=255) ggATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAAt < 2:1814651/76‑1 (MQ=255) ggATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAAt < 1:3217516/76‑1 (MQ=255) ggATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCggaa > 1:1332948/1‑73 (MQ=255) aTAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAAtgt < 2:2817319/76‑1 (MQ=255) aTAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAAtgt < 1:602133/76‑1 (MQ=255) tAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGGGGAtgtg > 1:2752440/1‑76 (MQ=255) aGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGa < 2:4202166/76‑1 (MQ=255) gCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGaa < 2:1801803/76‑1 (MQ=255) cttttttttAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAAt < 1:1523821/73‑1 (MQ=255) cgtttttttAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAAt < 1:56781/73‑1 (MQ=255) ttttttttAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATc < 1:2103254/74‑1 (MQ=255) tttttttAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATcc < 1:4510659/75‑1 (MQ=255) ttttttAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCg < 2:4387644/76‑1 (MQ=255) ttttttAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCg < 2:1547276/76‑1 (MQ=255) ttttTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGc < 2:718675/76‑1 (MQ=255) tttCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGGATGTGAATCCGc > 1:2429907/1‑76 (MQ=255) ttCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGcc > 1:2253991/1‑76 (MQ=255) | CAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG‑CTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTG > REL606/4392483‑4392631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |