| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 691,852 | 0 | T | G | 38.2% | 187.7 / 18.9 | 110 | intergenic (+17/‑33) | ybfM/ybfN | predicted outer membrane porin/predicted lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (33/35); new base G (42/0); total (75/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 162 total aligned reads displayed.
TCTTCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGCTGG > REL606/691777‑691927 | tCTTCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGt > 2:2195090/1‑76 (MQ=255) cTTCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGtt < 1:4300228/76‑1 (MQ=255) cTTCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGtt < 1:1415334/76‑1 (MQ=255) ttCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGttt > 2:4858898/1‑76 (MQ=255) tCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGcttt > 2:3858490/1‑76 (MQ=255) cAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTtgt > 1:3896922/1‑76 (MQ=255) cAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTtgt > 1:119485/1‑76 (MQ=255) aGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTtgtg > 1:970912/1‑76 (MQ=255) aGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTtgtg > 2:550099/1‑76 (MQ=255) ggATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGtgc > 2:1192930/1‑76 (MQ=255) aTGAGCGTGACGTAAACGTTACGCGAATCTGACCATTCACCTACTTCTGATGCCCGACAGGGCAATTTTTGtgcct < 2:4240255/76‑1 (MQ=255) aTGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGtgcct < 2:1948839/76‑1 (MQ=255) aTGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGtgcct > 1:867211/1‑76 (MQ=255) aTGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGtggct > 2:1825723/1‑76 (MQ=255) tGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGtggctg > 1:4218342/1‑76 (MQ=255) gCGTGCCGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑g < 2:1404451/76‑1 (MQ=255) gTGACGTAAAATTGATGGTAATCGCACCTTTCACCTTTTTCTGATGCCCGCCGCGGCGGGTTTTGTCCCTGCC‑GCa < 2:1146658/76‑1 (MQ=255) tGACGTAAATTTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCGGGTTTTGTGCCTGCC‑GCAc < 1:2973914/76‑1 (MQ=255) tGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCAc < 1:2413952/76‑1 (MQ=255) tGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTTTGCCTGAC‑Gccc > 1:2405404/1‑74 (MQ=255) gACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCACg > 1:671087/1‑76 (MQ=255) aCGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCACGt > 2:2023387/1‑76 (MQ=255) cgaaaatTTTAGGGAAACCGCCCCTTTCCCCATCTCCTGATCCCCGACGGGGCGGTTTTTGTCCCTGCC‑GCACGtt < 2:3014867/73‑1 (MQ=255) cGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGGC‑GCCCGtt > 1:1973518/1‑76 (MQ=255) cGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGtt < 2:2376267/76‑1 (MQ=255) cGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTTTGCCTGCC‑GCAccgt > 1:717055/1‑73 (MQ=255) gTAAATTTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTCCCTGCC‑GCACGttt < 2:4334281/76‑1 (MQ=255) gTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGttt < 2:2413568/76‑1 (MQ=255) gTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCCCGttt > 2:3740241/1‑76 (MQ=255) aaaaTTTTTTGTAATCTGTCCATTCACTTTTTTCATAGGCCCGACCAGGCAGGTTTGGTTCTTGCC‑GCACGTTTGa < 2:1890911/76‑1 (MQ=255) aaaaTTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGa < 2:2304158/76‑1 (MQ=255) aaaaTTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGGTTgg > 2:4818544/1‑75 (MQ=255) aaaaTTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTCCCTGCC‑GCACGTTTGa < 2:3117699/76‑1 (MQ=255) aaaaTTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTCCCTGCC‑GCACGTTTGa < 2:2077368/76‑1 (MQ=255) aaaaTTTATGGAATGCGCACATATCACCATCTTCTGACGCCCGCCGCGGCTGTTTTTGTGCCTGCC‑GCACGTTTGa < 2:4448110/76‑1 (MQ=255) aaaTTTATGGTAAGCGCGCCATGCCCCTTCTCCTGATGCCCGACCCGCAAGGTTTTGTGCCTGCC‑GCACGTTTGAg < 2:2529318/76‑1 (MQ=255) aTTTATGGTAATCGCCCCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGa < 2:799519/76‑1 (MQ=255) aTTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGa > 1:3367549/1‑76 (MQ=255) aTTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCCGGCCCGTTTggga > 1:1736075/1‑73 (MQ=255) aTTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCACGTTTGAGGa > 1:3500752/1‑76 (MQ=255) aTTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCACGGTTGAGGa > 2:76766/1‑76 (MQ=255) ttATGTTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAAt < 2:4673115/76‑1 (MQ=255) ttATGGTACTCTCACCATTCCCCATCTTCTGATGCCCGCCGGGTCCGGTTTTGGGCCCGCC‑GCACGTTTGAGGAAt < 2:4512486/76‑1 (MQ=255) ttATGGTAATCGCCCCATTCACCATCTTCTGAGGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAAt < 1:4760068/76‑1 (MQ=255) tATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAAtt < 2:1240607/76‑1 (MQ=255) tATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCACGTTTGAGGAAtt > 1:975312/1‑76 (MQ=255) aTGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑CCACGTTTGAGGGAttt > 2:781814/1‑76 (MQ=255) gTAATCGCCCCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCt < 2:1547441/76‑1 (MQ=255) gTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCt > 2:5060623/1‑76 (MQ=255) gTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCt > 1:4968695/1‑76 (MQ=255) gTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGGTTGAGGAATTTGCt > 2:3460219/1‑76 (MQ=255) gTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCACGTTTGAGGGATTTGCt > 2:774129/1‑76 (MQ=255) gTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCt > 2:2352408/1‑76 (MQ=255) tAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTa > 2:4476337/1‑76 (MQ=255) aaTCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCACGTTTGAGGGATTTGCTAt > 2:617575/1‑76 (MQ=255) aaTCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCACGTTTGAGGGATTTGCTAt > 2:1746693/1‑76 (MQ=255) aTCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCCCGTTTGAGGAATTTGCTATg > 1:2288501/1‑76 (MQ=255) aTCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGGATTTTCTATg > 2:2898396/1‑76 (MQ=255) aTCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCCATg > 2:827056/1‑76 (MQ=255) tCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGGTCCTGCC‑GCCCGGTTGAGGGATTTGCTATGa > 1:791936/1‑76 (MQ=255) gCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGaaa > 2:4383561/1‑76 (MQ=255) cACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGaaaa < 2:3586632/76‑1 (MQ=255) cACCATTCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCCCGTTTGAGGGATTTGCTATGaaaa > 2:2712366/1‑76 (MQ=255) aCCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGGATTTGCTATGaaaaa > 1:1010360/1‑76 (MQ=255) cctttCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGaaaaaa < 2:4652393/73‑1 (MQ=255) ctttCCCCTTCTTCGGACCCCCGCCGGGGGTGTTTTTGTCCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAAc < 1:1730788/74‑1 (MQ=255) ttCACCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTg > 2:1481553/1‑76 (MQ=255) cACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAAATGAt > 2:3601396/1‑76 (MQ=255) aCCATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAAATGAtt > 2:3307469/1‑76 (MQ=255) ccATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATtc > 1:1945358/1‑76 (MQ=255) cATCTTCTGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑GCCCGGTTGAGGAATTTGCTATGAAAAAACTGAtttt > 1:4335640/1‑74 (MQ=255) cttctGATGCACGACGCGGCAGGTTTTGTGCCTGCC‑GCCCCTTTGAGGAATTTGCCATGAACAAAATGAATCTAAt > 1:3858462/1‑76 (MQ=255) ttctGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATc < 2:2037101/76‑1 (MQ=255) ttctGATGCCCGACGCGGCAGGGTTTGTGCCTGCC‑CCCCGGTTTAGGGATTTGCTATGAAAAAACTGGTTTTCATc > 2:4875332/1‑76 (MQ=255) tctGATGCCCGACGCGGCAGGTTTTGTGGCTGCC‑GCACGGTTGAGGGATTTGCTATGAAAAAAATGATTCTCAtgg > 2:690177/1‑74 (MQ=255) tctGATGCCCGACGCGGCAGGGTTTGGGGCCGCC‑GCACGGTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCg > 1:1066298/1‑76 (MQ=255) ctGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGc > 1:3031785/1‑76 (MQ=255) ctGANGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGc > 2:3797640/1‑76 (MQ=255) tccccGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCatg < 2:2370070/74‑1 (MQ=255) tGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCatg > 2:4968799/1‑76 (MQ=255) cccGACGCGGCAGGTTTTGTCCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCatgat < 1:4876165/76‑1 (MQ=255) ccgccgCGGGAGTTTTTGTCCCCGCC‑GAACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCatgatg < 1:827056/76‑1 (MQ=255) ccGACGCGGCAGGTTTTGTGCCTGCC‑GCACCTTTGAGGAATTTGCTATGAAAAAACTGATTGTCATCGCCatgatg > 2:4392395/1‑76 (MQ=255) cgccgcgGCAGTTTTTGTGCCGGCC‑TCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATgg < 2:3524317/73‑1 (MQ=255) cGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATgg < 2:4442387/76‑1 (MQ=255) cGACGCGGCAGGGTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATgg > 2:1178650/1‑76 (MQ=255) cGACGCGGCAGGGTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTAATCGCCATGATgg > 2:1032699/1‑76 (MQ=255) gACGCGGCAGGTTTTGGGCCTGCC‑GCACGGTTGAGGAATTTGCTCTGAAAAAACTGATTCTCATCGCCATGATGGc > 2:2723988/1‑76 (MQ=255) gACGCGGCAGGGTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGc > 2:4060019/1‑76 (MQ=255) cgcgGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCAt < 1:2515546/76‑1 (MQ=255) cgGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCg > 1:1311029/1‑76 (MQ=255) ggCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCgg > 2:110882/1‑76 (MQ=255) gcgggTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCggg < 2:2110465/73‑1 (MQ=255) gcgggTTTGGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCggg < 2:1395498/73‑1 (MQ=255) cAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCgggg > 2:2925137/1‑76 (MQ=255) aGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGc < 2:1034224/76‑1 (MQ=255) aGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGc > 2:620768/1‑76 (MQ=255) gTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGCTg > 1:806666/1‑76 (MQ=255) ttttGTGCCTGCC‑GCACGTTTGAGGAATTTGTTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGCTgg > 1:3710597/1‑76 (MQ=255) tttgGTCCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGCTgg < 2:1329173/76‑1 (MQ=255) | TCTTCCAGGATGAGCGTGACGTAAAATTTATGGTAATCGCACCATTCACCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCC‑GCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATGATGGCATCGGGGCTGG > REL606/691777‑691927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |