| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,096,032 | 0 | C | G | 39.7% | 93.2 / 57.8 | 68 | G148G (GGC→GGG) | putP | proline:sodium symporter |
| Reads supporting (aligned to +/- strand): ref base C (3/38); new base G (27/0); total (30/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.96e-16 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTGCGCATTATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGCTCTGTGGGCGGGAGCTGCGGCGACGATCCTT > REL606/1095960‑1096107 | tttGCGCATTATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGgcgcg < 1:993847/76‑1 (MQ=255)tttGCGCATTATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGgcgcg < 1:2995084/76‑1 (MQ=255)tttGCGCATTATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTAGCAGgcgcg < 1:962233/76‑1 (MQ=255) ttGCGCATTATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGgcgcgg > 2:1244625/1‑75 (MQ=255) ttGCGCATTATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGgcgcgc < 2:2485457/76‑1 (MQ=255) tGCGCATTATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAggcgggcg > 2:297548/1‑76 (MQ=255) tGCGCATTATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGggcgcg > 1:1750931/1‑76 (MQ=255) gcgcATTATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGt < 2:829322/76‑1 (MQ=255) cgcATTATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGCGGGTc > 2:4648410/1‑76 (MQ=255) gcATTATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGCGCGGCt > 1:3824772/1‑76 (MQ=255) cATTATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTg < 2:3439605/76‑1 (MQ=255) aTTATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGCGCGGCTG‑t > 2:4676829/1‑76 (MQ=255) ttATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGCCGCTG‑tt > 2:2153221/1‑76 (MQ=255) ttATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑tt < 1:4625019/76‑1 (MQ=255) tATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGCGGCTG‑ttt > 2:628914/1‑76 (MQ=255) tATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGCGGGGCTG‑ttt > 1:3925549/1‑76 (MQ=255) tATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑ttt < 1:1936202/76‑1 (MQ=255) aTCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGGGGCTG‑TTTg > 1:3744745/1‑76 (MQ=255) aTCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGCGGCTG‑tttt > 1:286010/1‑75 (MQ=255) aTCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGCGGCTG‑tttt > 1:4945516/1‑75 (MQ=255) aTCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTg < 1:110798/76‑1 (MQ=255) tctcCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGCGGCTG‑TTTgt > 2:1001175/1‑75 (MQ=255) ctcCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGCGCGTCTG‑cttcta > 2:3641854/1‑73 (MQ=255) tcCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGaaa < 1:2564123/76‑1 (MQ=255) tcCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGaaa < 1:4262923/76‑1 (MQ=255) ccGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAg < 2:2888250/76‑1 (MQ=255) gcgcTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTa < 2:3801509/76‑1 (MQ=255) cgcTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTAc < 2:2067254/76‑1 (MQ=255) cTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGGGGCTG‑TTTTTAAGTACCt > 1:2579574/1‑76 (MQ=255) tGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGCGGCTG‑TTTTTAAATACCtt > 1:4185046/1‑76 (MQ=255) tGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGCGGCTG‑TTTGAAAGTACCtt > 2:1970292/1‑76 (MQ=255) ggTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCttt < 2:3865567/76‑1 (MQ=255) gTGATTTTGCTGTTCTTCGCCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTg < 1:1033728/76‑1 (MQ=255) gTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGCGGCCG‑TTTTGTAGTACCtttt > 1:3842301/1‑75 (MQ=255) gTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTg < 2:4524455/76‑1 (MQ=255) tGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGGGGCGG‑TTTTTTAGTACCTTTgt > 1:1569560/1‑75 (MQ=255) tGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGCGTCTG‑TTTGTAAGTACCttttg > 2:1587403/1‑74 (MQ=255) tGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTgg < 2:4959955/76‑1 (MQ=255) gATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGGGGCTGTTTTGAAAG‑ACCttttgg > 1:4188494/1‑73 (MQ=255) gATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGCGCGGCTC‑TTTGTAAGTACCTTTTGc > 2:3861642/1‑76 (MQ=255) aTTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGCGCGGGGG‑TTTGTAAGTACCTTTGTCa > 1:4533848/1‑76 (MQ=255) ttttGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCAt < 1:4970569/76‑1 (MQ=255) ttttGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCAt < 1:4195785/76‑1 (MQ=255) tttGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGGCGCTG‑TTTTGTAGTACCTTTCGCCTg > 1:1609748/1‑76 (MQ=255) tttGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGCGGCTG‑TTTTTAAGTACCTTTTGCacg > 1:40218/1‑74 (MQ=255) tttGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATg < 2:4305880/76‑1 (MQ=255) tGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGGGGCTG‑TTTGTAAGTACCTTTGGCATggg > 1:2495806/1‑74 (MQ=255) cTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCCCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCt < 2:526690/76‑1 (MQ=255) tGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTa < 2:3936812/76‑1 (MQ=255) tGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTa < 2:1884360/76‑1 (MQ=255) ttcttcACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGGGGCTG‑TTTTTAAATAACTTTTGGATGAGCTAca > 2:50722/1‑75 (MQ=255) ttcttcACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACg < 1:4780517/76‑1 (MQ=255) tcttcACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑a < 2:136801/76‑1 (MQ=255) tcttcACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑a < 1:4901800/76‑1 (MQ=255) cttcACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑aa < 2:2721110/76‑1 (MQ=255) cttcACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑aa < 2:4264372/76‑1 (MQ=255) ttcACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑aaa < 1:3790081/76‑1 (MQ=255) tcACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGGGGCCG‑GTTTTAAGTACCCTTTGCATGGGCTACG‑caaa > 1:474186/1‑75 (MQ=255) tcACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGCGGCTG‑TTTTTAAGTACCTTTTGCATGAGCTACG‑GAAc > 2:1718912/1‑76 (MQ=255) cACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGCGGCTG‑TTTGGAAGTAACTTTGGCATGAGCTACG‑caaaa > 2:1446297/1‑74 (MQ=255) cACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGCGCGGCTG‑TTTTTAAGTAACTTTGGCATGAGCTACG‑AAAca > 2:2624189/1‑75 (MQ=255) cACCATTTATTGCGCTTCGGGCATTGTGGCAGGGGCGCGGCTG‑TTTTGAAGGACCTTTGGTGTGAGGTACG‑GAACg > 1:3302046/1‑76 (MQ=255) cACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACg < 2:3910911/76‑1 (MQ=255) cACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACg < 2:3321357/76‑1 (MQ=255) cACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACg < 2:1952340/76‑1 (MQ=255) aCCATTTATTGCGCTTCGGGCATTGTGGCAGGGGGGGGGCCG‑TTTGGAAGTAACTTTTGCATGAGCTACG‑ACACgg > 1:4708439/1‑76 (MQ=255) aCCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGGGGCTT‑GTTGATAGGATCTATGGGATGAGATATG‑AAAcag > 1:4104790/1‑74 (MQ=255) aCCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACgg < 1:1659392/76‑1 (MQ=255) ccATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGc < 2:4636327/76‑1 (MQ=255) cATTTATTGCGCTTCGGGCATTGTGGCAGGGGCGCGGCTG‑TTTGGAAGGAACCTTGGTGTGGACTACG‑CAAAGGct > 1:2765346/1‑76 (MQ=255) aTTTATTGCGCTTCGGGCATTGTGGCAGGGGGGCGGCTG‑TTTTTAAGAAACTTTTGCATGAGCTACG‑AAACAGctc > 2:1511708/1‑76 (MQ=255) aTTTATTGCGCTTCGGGCATTGTGGCAGGGGCGCGGCTG‑TTTGTAAGTACCTTTGGCATGAGCTACG‑CAACGGcgc > 2:2536499/1‑74 (MQ=255) tATTGCGCTTCGGGCATTGTGGCAGGGGGGCGGCTG‑TTTTTAAGTACCTTTTGCATGAGCTAAG‑AAACGGCTCtgt > 2:3524549/1‑76 (MQ=255) tATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGCTCtgt < 1:3983419/76‑1 (MQ=255) atggcgcTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGCTCtgtg < 1:5000364/73‑1 (MQ=255) aTTGCGCTTCGGGCATTGTGGCAGGGGGGGGGCTG‑TTTTAAAGGAACCTTTGCATGGGATACA‑AAACGGCGCtggg > 2:1997733/1‑74 (MQ=255) aTTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGCTCtgtg < 2:3171514/76‑1 (MQ=255) tGCGCTTCGGGCATTGTGGCAGGGGGGGGCCTG‑TTTGTAAGTCCCTTTTGCTTGAGCTACG‑AAAAGGATCTGTggg > 2:2678416/1‑76 (MQ=255) tGCGCTTCGGGCATTGTGGCAGGGGGGCGGCTG‑TTTTTAAGGACCTTTGGCCTGAGCTACG‑AAACGGCTCTGTggg > 1:4777433/1‑76 (MQ=255) tGCGCTTCGGGCATTGTGGCAGGGGGGCGGCTG‑TTTTGAAGAAACTTTTGCCTGGGGGACG‑AAAAGGGTCCCTggg > 2:1022647/1‑76 (MQ=255) tGCGCTTCGGGCATTGTGGCAGGGGGGCGGCTG‑TTTGTAAGTACCTTTGGCATGAGCGACG‑AAACGGCTCCTTggg > 1:4077048/1‑76 (MQ=255) gcgcTTCGGGCATTGTGGCAGGGGCGCGGCTG‑TTTTTAAAGACCCTTTGCATGAGCTACG‑AAAAGGGGCTGggggg > 1:434841/1‑75 (MQ=255) tCGGGCATTGTGGCAGGGGGGGGGGTG‑TTTTTAAGTACCCTTGTGTTGAGCTACG‑AAAACGCTCTTTGgggggggg > 2:1507948/1‑74 (MQ=255) cGGGCATTGTGGCAGGGGGGCGGCTGTTTTTAAAAAACCTTTGG‑ATTTGGTACGAAAACGGC‑CTGTGggggggggg > 1:3403462/1‑73 (MQ=255) cGGGCATTGTGGCAGGGGGGCGCCTGTTTTTAAACAACCTTT‑GCATGAGGTACG‑CAACGGCTCCGTGggggggggg > 2:204426/1‑73 (MQ=255) gggCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGCTCTGTGGGCGGGAGCt < 2:667560/76‑1 (MQ=255) tgtgGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGCTCTGTGGGCGGGAGCTgcggcg < 2:1363781/76‑1 (MQ=255) tgGCAGGCGCGCGTCTT‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGCTCTGTGGGCGGGAGCTGCGGcgac > 2:2038860/1‑76 (MQ=255) tgGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGCTCTGTGGGCGGGAGCTGCGGcgac < 1:965549/76‑1 (MQ=255) tgGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGCTCTGTGGGCGGGAGCTGCGGcgac < 2:1414115/76‑1 (MQ=255) ggCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGCTCTTTGGGCGGGAGCTgcggcggcg > 1:663655/1‑76 (MQ=255) cAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGCTCTGTGGGCGGGAGCTGCGGCGACGAt < 2:4289195/76‑1 (MQ=255) aGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGCTCTGTGGGCGGGAGCTGCGGCGACGATc > 2:2383399/1‑76 (MQ=255) cgcgcgTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGCTCTGTGGGCGGGAGCTGCGGCGACGATCCtt > 1:5109874/1‑76 (MQ=255) | TTTGCGCATTATCTCCGCGCTGGTGATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTG‑TTTGAAAGTACCTTTGGCATGAGCTACG‑AAACGGCTCTGTGGGCGGGAGCTGCGGCGACGATCCTT > REL606/1095960‑1096107 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |