| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,941,586 | G→A | V396M (GTG→ATG) | wecF → | putative enterobacterial common antigen polymerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,941,586 | 0 | G | A | 99.4% | 690.2 / ‑6.2 | 164 | V396M (GTG→ATG) | wecF | putative enterobacterial common antigen polymerase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (90/73); total (91/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 165 total aligned reads displayed.
CAACCGCGAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGGCGCATGTC > REL606/3941513‑3941658 | cAACCGCGAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATg < 2:244998/76‑1 (MQ=255) aaCCGCGAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGc < 2:3702383/76‑1 (MQ=255) aaCCGCGAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGc < 2:4970837/76‑1 (MQ=255) ccGCGAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTg < 2:2684212/76‑1 (MQ=255) ccGCGAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTg < 1:978327/76‑1 (MQ=255) cgcgAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTgg < 1:5015861/76‑1 (MQ=255) cgAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTggag > 2:4371194/1‑74 (MQ=255) gaCTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGt > 2:3909897/1‑76 (MQ=255) gaCTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGt < 2:325668/76‑1 (MQ=255) gaCTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGt > 1:1364067/1‑76 (MQ=255) aCTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTg < 1:1700972/76‑1 (MQ=255) cTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGa > 2:1247612/1‑76 (MQ=255) tAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGaa < 1:4936749/76‑1 (MQ=255) tAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGaa < 1:4356055/76‑1 (MQ=255) tAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGaa > 2:3814648/1‑76 (MQ=255) tAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGaa < 2:1529706/76‑1 (MQ=255) aaTCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAg < 1:3090544/76‑1 (MQ=255) aaTCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAg < 2:695900/76‑1 (MQ=255) aTCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAgg < 1:1876887/76‑1 (MQ=255) aTCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAgg > 2:3432852/1‑76 (MQ=255) cGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGc > 1:2709680/1‑76 (MQ=255) cGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGc > 2:4031086/1‑76 (MQ=255) gCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCt < 2:1486057/76‑1 (MQ=255) gCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCt < 1:3728674/76‑1 (MQ=255) gCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCt > 2:2838348/1‑76 (MQ=255) cTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTg > 1:399729/1‑76 (MQ=255) cTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTg < 2:3025130/76‑1 (MQ=255) cTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTg < 2:2669298/76‑1 (MQ=255) cTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTg < 1:3481290/76‑1 (MQ=255) cTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTg > 1:3162498/1‑76 (MQ=255) cTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTg > 1:287484/1‑76 (MQ=255) tataAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTgg < 2:239962/76‑1 (MQ=255) tataAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTgg > 1:616032/1‑76 (MQ=255) tataAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTgg > 1:4894828/1‑76 (MQ=255) tataAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTgg < 1:4320095/76‑1 (MQ=255) ataAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGa < 2:3817392/76‑1 (MQ=255) ataAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGa > 1:3880635/1‑76 (MQ=255) ataAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGa > 2:4401655/1‑76 (MQ=255) taAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGAt > 1:4158648/1‑76 (MQ=255) taAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGAt > 2:4224225/1‑76 (MQ=255) aaGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGGtt > 1:431314/1‑76 (MQ=255) aGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTc > 1:4680721/1‑76 (MQ=255) aGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTc < 1:1052940/76‑1 (MQ=255) aGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTc < 1:3621516/76‑1 (MQ=255) aGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTc < 1:2745273/76‑1 (MQ=255) cTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGtt > 1:2816884/1‑76 (MQ=255) cTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGtt < 2:1185525/76‑1 (MQ=255) cTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGtt < 2:2397785/76‑1 (MQ=255) tGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGttt > 1:82195/1‑76 (MQ=255) gCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTg < 2:2240819/76‑1 (MQ=255) cGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGt > 2:3482370/1‑76 (MQ=255) cGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGt > 2:1460366/1‑76 (MQ=255) atatTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGtct > 1:3455067/1‑76 (MQ=255) atatTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGtct < 2:5016568/76‑1 (MQ=255) atatTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGtct > 1:1849446/1‑76 (MQ=255) tatTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGtctc < 1:2272595/76‑1 (MQ=255) atTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCa < 2:775152/76‑1 (MQ=255) ttACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAc < 2:4508399/76‑1 (MQ=255) ttACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAc < 2:4237571/76‑1 (MQ=255) tACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAcg < 1:74601/76‑1 (MQ=255) acacAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAcgc > 2:3081705/1‑76 (MQ=255) acacAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAcgc > 2:4728655/1‑76 (MQ=255) acacAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAcgc > 1:2148954/1‑76 (MQ=255) acacAGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAcgc > 2:4657447/1‑76 (MQ=255) cacaGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCAcgcg < 2:129401/76‑1 (MQ=255) aGTTTCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTgg > 2:4498483/1‑76 (MQ=255) tttCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTc > 1:3392659/1‑76 (MQ=255) ttCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCt > 1:2635064/1‑76 (MQ=255) ttCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCt > 1:1805413/1‑76 (MQ=255) ttCTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCt < 1:1736620/76‑1 (MQ=255) cTGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCttt > 1:958988/1‑76 (MQ=255) tGCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGGCtttt > 1:1326276/1‑76 (MQ=255) gCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCttttt > 1:2117193/1‑76 (MQ=255) gCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCttttt > 2:4972825/1‑76 (MQ=255) gCTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGCCttttt > 1:4540972/1‑76 (MQ=255) cTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCtttttt < 2:3647609/76‑1 (MQ=255) cTTCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCtttttt < 1:1104105/76‑1 (MQ=255) tCGGGGCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTAt > 2:3854787/1‑76 (MQ=255) gCGATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTgg > 2:2209438/1‑76 (MQ=255) gATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTc > 2:943843/1‑76 (MQ=255) gATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTc < 1:1578041/76‑1 (MQ=255) gATCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTc > 2:1783773/1‑76 (MQ=255) aTCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCt > 2:4399082/1‑76 (MQ=255) aTCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCt > 1:2940499/1‑76 (MQ=255) aTCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCt > 1:4661707/1‑76 (MQ=255) tCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCtt < 2:1723739/76‑1 (MQ=255) tCTTCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCtt < 1:1154716/76‑1 (MQ=255) ttCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCg < 2:150844/76‑1 (MQ=255) ttCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCg > 1:2564779/1‑76 (MQ=255) ttCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCg > 1:1243065/1‑76 (MQ=255) tCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCgg < 1:4783501/76‑1 (MQ=255) tCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCgg > 1:2893292/1‑76 (MQ=255) tCAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCgg < 1:1306213/76‑1 (MQ=255) cAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGgc > 2:360904/1‑76 (MQ=255) cAATATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCATGGTCTTCGgc > 2:2954857/1‑76 (MQ=255) aaTATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGgcg > 1:4550759/1‑76 (MQ=255) aaTATGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGgcg > 1:4209075/1‑76 (MQ=255) tatGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGGCTCa < 1:2045147/76‑1 (MQ=255) tatGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGGCGCa > 1:4236813/1‑76 (MQ=255) tatGATCATGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGGCGCa < 2:2209089/76‑1 (MQ=255) | CAACCGCGAGACTAATCGCTATAAGGCTGCGATATTACACAGTTTCTGCTTCGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGGCGCATGTC > REL606/3941513‑3941658 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |