| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,108,375 | C→T | R43H (CGT→CAT) | metJ ← | transcriptional repressor protein MetJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,108,375 | 0 | C | T | 98.7% | 622.0 / ‑1.9 | 153 | R43H (CGT→CAT) | metJ | transcriptional repressor protein MetJ |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (78/73); total (79/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
Only 100 of 166 total aligned reads displayed.
TAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCAC > REL606/4108304‑4108447 | tAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATgcgt > 1:30420/1‑76 (MQ=255) aaaGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATgcgtg > 2:441090/1‑76 (MQ=255) aaaGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATgcgtg > 1:3372862/1‑76 (MQ=255) aaGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATgcgtgc > 1:545453/1‑76 (MQ=255) aaGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATgcgtgc > 1:1372620/1‑76 (MQ=255) aaGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATgcgtgc > 2:1264036/1‑76 (MQ=255) aGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGAtgcgtgcg > 1:2067818/1‑76 (MQ=255) ggCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcgt > 1:2668563/1‑76 (MQ=255) cATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTc < 1:4418675/76‑1 (MQ=255) cATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTc > 1:2890713/1‑76 (MQ=255) cATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTc < 1:2713799/76‑1 (MQ=255) cATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTc < 1:2347607/76‑1 (MQ=255) aTGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCa > 2:204167/1‑76 (MQ=255) aTGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCa > 2:3617156/1‑76 (MQ=255) tGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCAt > 2:1712196/1‑76 (MQ=255) gCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATc < 1:1783955/76‑1 (MQ=255) gCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATc > 1:1688582/1‑76 (MQ=255) cAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCg < 1:4831549/76‑1 (MQ=255) aGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCgg < 1:675570/76‑1 (MQ=255) gAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGt > 2:365322/1‑76 (MQ=255) aaaCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTg < 2:1575322/76‑1 (MQ=255) aaCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCCCCTGACGATGCGTGCGTTCATCGGTGa < 1:4514966/76‑1 (MQ=255) aaCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGa < 2:2035422/76‑1 (MQ=255) aaCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGa < 2:3367906/76‑1 (MQ=255) aaCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGa > 1:473779/1‑76 (MQ=255) aaCGCTTCGCACAGCAGCTCGCTGTTGGAAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGa > 1:2204012/1‑76 (MQ=255) aCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAg < 1:1889431/76‑1 (MQ=255) cGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAgg > 1:4130453/1‑76 (MQ=255) cTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGAt > 2:2878279/1‑76 (MQ=255) cTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGAt < 2:4910126/76‑1 (MQ=255) ttCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGAtt > 2:672589/1‑76 (MQ=255) tCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGAttt < 1:3820476/76‑1 (MQ=255) cGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAtttt < 1:2945669/76‑1 (MQ=255) cacaGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTa < 1:1008129/76‑1 (MQ=255) cacaGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTa < 1:61778/76‑1 (MQ=255) cacaGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTa < 2:548631/76‑1 (MQ=255) acaGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTaa > 2:4930477/1‑76 (MQ=255) acaGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTaa > 1:4049086/1‑76 (MQ=255) cagcagCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAac < 1:3452010/76‑1 (MQ=255) agcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCt < 1:3145436/76‑1 (MQ=255) gcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCtt > 2:4489749/1‑76 (MQ=255) gcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCtt < 2:369311/76‑1 (MQ=255) gcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCtt > 2:3467570/1‑76 (MQ=255) gcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCta < 2:1156496/76‑2 (MQ=255) cTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTa > 1:3495176/1‑76 (MQ=255) cTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTa < 2:3952975/76‑1 (MQ=255) cTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTa < 2:1298105/76‑1 (MQ=255) cTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTa < 1:477859/76‑1 (MQ=255) cTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTa > 2:1439535/1‑76 (MQ=255) tCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTaa < 2:3921025/76‑1 (MQ=255) cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAag > 2:4601952/1‑76 (MQ=255) cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAag > 2:3405224/1‑76 (MQ=255) cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAag > 1:4913145/1‑76 (MQ=255) cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAag > 2:4586037/1‑76 (MQ=255) cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAag < 1:4593260/76‑1 (MQ=255) gCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAaga > 1:940468/1‑76 (MQ=255) gCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAaga > 2:4366088/1‑76 (MQ=255) gCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAaga > 2:2151512/1‑76 (MQ=255) cTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGGTTTTTAACACCTTAagag > 2:1349576/1‑76 (MQ=255) cTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAagag < 1:4274791/76‑1 (MQ=255) cTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAagag > 1:4915929/1‑76 (MQ=255) cTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAagag > 1:3571132/1‑76 (MQ=255) gTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGa > 2:3504849/1‑76 (MQ=255) gTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGa > 1:573135/1‑76 (MQ=255) gTTGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGa > 2:1630922/1‑76 (MQ=255) tGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAAt < 1:2826014/76‑1 (MQ=255) tGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAAt < 2:2892522/76‑1 (MQ=255) tGGTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAAt < 1:2849341/76‑1 (MQ=255) tGGTAGCGTGACGCAGGTTGTTCACCCGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAAt > 1:2564131/1‑76 (MQ=255) ggTAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATg < 2:32529/76‑1 (MQ=255) tAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa < 1:5075708/76‑1 (MQ=255) tAGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa > 2:2136896/1‑76 (MQ=255) aGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGaa < 1:4373844/76‑1 (MQ=255) aGCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGaa > 1:111124/1‑76 (MQ=255) gCGTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGaaa < 1:446381/76‑1 (MQ=255) gTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAcc < 1:685617/76‑1 (MQ=255) gTGACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAcc > 1:990598/1‑76 (MQ=255) gACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGt < 1:3367994/76‑1 (MQ=255) gACGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGt < 2:3782708/76‑1 (MQ=255) aCGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTa < 1:822087/76‑1 (MQ=255) cGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTaa > 2:2502635/1‑76 (MQ=255) cGCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTaa < 1:4722692/76‑1 (MQ=255) gCAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAAt < 1:1514114/76‑1 (MQ=255) cAGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATc > 2:3023827/1‑76 (MQ=255) gttgttCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCttt > 1:1466166/1‑76 (MQ=255) gttgttCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCttt < 2:4225327/76‑1 (MQ=255) ttgttCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCtttt > 1:1573808/1‑76 (MQ=255) tgttCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCttttt > 2:1500237/1‑76 (MQ=255) tgttCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCttttt < 1:4159377/76‑1 (MQ=255) tCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGAc > 1:4597043/1‑76 (MQ=255) aCCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACtt < 1:3933200/76‑1 (MQ=255) ccTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTg > 2:4805448/1‑76 (MQ=255) cTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACGCCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGt > 1:1271123/1‑76 (MQ=255) cTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGt < 2:5013064/76‑1 (MQ=255) tGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGtt < 2:619398/76‑1 (MQ=255) gacgatGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTc < 2:3063466/76‑1 (MQ=255) gacgatGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTc > 1:1243241/1‑76 (MQ=255) gacgatGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTc < 2:2798873/76‑1 (MQ=255) acgatgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCa < 2:1002443/76‑1 (MQ=255) cgatgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCAc < 2:3544476/76‑1 (MQ=255) | TAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCAC > REL606/4108304‑4108447 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |