| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,163,127 | C→T | R758C (CGT→TGT) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,163,127 | 0 | C | T | 98.7% | 631.1 / ‑1.5 | 154 | R758C (CGT→TGT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (91/61); total (92/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
Only 100 of 155 total aligned reads displayed.
TATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACG > REL606/4163054‑4163199 | tATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAGCCTGACCAAATACACCTGt > 1:1192775/1‑76 (MQ=255)tATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGt > 1:3956248/1‑76 (MQ=255)tATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGt < 1:3030523/76‑1 (MQ=255) aTCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGtt > 2:3501633/1‑76 (MQ=255) aTCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGtt > 1:4502274/1‑76 (MQ=255) aTCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGtt > 1:3006935/1‑76 (MQ=255) aTCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGtt > 1:703317/1‑76 (MQ=255) cAAAGTTAACGAAGACGAGATGTATCCGTGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCt < 2:2869588/76‑1 (MQ=255) cAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCt < 2:4981133/76‑1 (MQ=255) cAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCt < 2:2706840/76‑1 (MQ=255) cAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCt > 2:2091969/1‑76 (MQ=255) aaaGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTa > 1:4262222/1‑76 (MQ=255) aaaGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTa < 2:3947991/76‑1 (MQ=255) aaaGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTa < 2:3300601/76‑1 (MQ=255) aaaGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTa < 1:1449106/76‑1 (MQ=255) aGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAAc > 1:1912704/1‑76 (MQ=255) aGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAAc < 2:5111847/76‑1 (MQ=255) aGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAAc > 2:1176772/1‑76 (MQ=255) gTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAAcc < 1:2421043/76‑1 (MQ=255) gTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAAcc > 1:3331735/1‑76 (MQ=255) tAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAg > 2:2845190/1‑76 (MQ=255) aaCGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGa < 1:4659263/76‑1 (MQ=255) aaCGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGa > 1:4561542/1‑76 (MQ=255) cGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAac > 2:1787511/1‑76 (MQ=255) gAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAaca > 1:3926854/1‑76 (MQ=255) gAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAaca > 2:2780663/1‑76 (MQ=255) aaGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAacac > 2:525875/1‑76 (MQ=255) aaGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAacac < 1:3118122/76‑1 (MQ=255) aGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACAcc > 2:1219205/1‑76 (MQ=255) gACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACAcca < 2:1377069/76‑2 (MQ=255) gataTGCATCCGGATGAAGCAGGTATCGACATCTACATCCTGACCAAATACCCCTGTTCTAACCAGAACACCTTTa < 2:1347659/73‑1 (MQ=255) gagaTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTa > 2:3084689/1‑76 (MQ=255) gagaTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTa > 2:2527127/1‑76 (MQ=255) gagaTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTa > 2:2439852/1‑76 (MQ=255) gaTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATc < 1:3973505/76‑1 (MQ=255) aTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCa < 1:4413575/76‑1 (MQ=255) aTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCa < 1:103998/76‑1 (MQ=255) tGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCaa < 2:253540/76‑1 (MQ=255) tGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCaa > 2:4018622/1‑76 (MQ=255) tGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCaa < 1:3754394/76‑1 (MQ=255) tATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAAcc > 2:158293/1‑76 (MQ=255) tATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAAcc > 2:3063547/1‑76 (MQ=255) aTCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCa > 2:3906376/1‑76 (MQ=255) ccGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGa > 1:4626635/1‑76 (MQ=255) ccGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGa > 2:2612821/1‑76 (MQ=255) cGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGAt < 2:577332/76‑1 (MQ=255) gggTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATg < 1:1815886/76‑1 (MQ=255) gAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCgt < 2:580661/76‑1 (MQ=255) gAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCgt > 2:832763/1‑76 (MQ=255) gAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCgt > 2:3231429/1‑76 (MQ=255) aaGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCgtg > 1:954873/1‑76 (MQ=255) aaGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCgtg > 1:2049941/1‑76 (MQ=255) aGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCgtgt > 1:2749585/1‑76 (MQ=255) cAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCgtgtgt > 1:1551715/1‑76 (MQ=255) gTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtc < 1:1155331/76‑1 (MQ=255) gTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtc < 1:4282160/76‑1 (MQ=255) gTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtc > 1:3466290/1‑76 (MQ=255) tATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtct > 1:1032408/1‑76 (MQ=255) aTCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGtctc > 1:767166/1‑76 (MQ=255) aTCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtctc < 1:2595159/76‑1 (MQ=255) aTCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtctc > 1:4954978/1‑76 (MQ=255) tCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTctct < 1:4161250/76‑1 (MQ=255) tCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTctct > 2:4858518/1‑76 (MQ=255) cGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTg > 1:2584087/1‑76 (MQ=255) cGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTg < 1:2689516/76‑1 (MQ=255) cATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGt > 1:240138/1‑76 (MQ=255) cATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGt < 1:4275282/76‑1 (MQ=255) aTCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTg > 2:2042861/1‑76 (MQ=255) aTCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTg > 2:4042384/1‑76 (MQ=255) aTCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTg > 1:3931051/1‑76 (MQ=255) tCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGa > 1:2806126/1‑76 (MQ=255) tCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGa < 1:124433/76‑1 (MQ=255) tCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGa < 1:2052610/76‑1 (MQ=255) cTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGaa < 1:2207921/76‑1 (MQ=255) cTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGaa > 2:4632654/1‑76 (MQ=255) tACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAc > 2:1702084/1‑76 (MQ=255) tACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAc > 2:3152066/1‑76 (MQ=255) tACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAc > 2:1444030/1‑76 (MQ=255) aCAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAcc > 1:1348498/1‑76 (MQ=255) cAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCg > 2:3870223/1‑76 (MQ=255) cAACCGGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCg > 2:964501/1‑76 (MQ=255) aCCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGt > 1:4378763/1‑76 (MQ=255) cTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTg < 2:3828975/76‑1 (MQ=255) cTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTg > 2:845176/1‑76 (MQ=255) cTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTg < 1:1922930/76‑1 (MQ=255) tGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGa > 2:2159441/1‑76 (MQ=255) tGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGa > 2:4452912/1‑76 (MQ=255) gACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGaa > 1:84334/1‑76 (MQ=255) aCCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAAc < 2:4228758/76‑1 (MQ=255) ccAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACg < 1:4785579/76‑1 (MQ=255) aaaTACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTg > 2:223169/1‑76 (MQ=255) aaaTACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTg > 1:967073/1‑76 (MQ=255) aaTACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTgg < 2:1685083/76‑1 (MQ=255) aaTACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTgg > 1:1505143/1‑76 (MQ=255) aTACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGc > 1:3486694/1‑76 (MQ=255) cacCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGAc < 1:1441213/76‑1 (MQ=255) cacCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGAc > 1:2347957/1‑76 (MQ=255) cacCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGAc > 2:3946946/1‑76 (MQ=255) acCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACg < 1:4566169/76‑1 (MQ=255) acCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACg > 2:2425573/1‑76 (MQ=255) | TATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACG > REL606/4163054‑4163199 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |