| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,193,779 | 0 | G | T | 99.0% | 364.7 / NA | 99 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (38/59); minor base C (0/1); total (38/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 116 total aligned reads displayed.
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851 | gCCGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GG‑GAAACAGCCCCAAAAAAAACAAAAAGCCAAAcc > 1:1416213/1‑76 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GG‑GAAACAGCCCCCAAAAAAGCAAAACACCAAAcccc > 1:2629129/1‑76 (MQ=255) aTGCATCCGGCGTCCTGGCGCGGCTGGTCTGGCGGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCccac < 1:622634/76‑1 (MQ=255) aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCccac < 1:3970546/76‑1 (MQ=255) tGCATCCGGCGTCCAGGCGCGGCTGGGCTGGGTGTC‑GG‑GAAACAGCCCCAATAACAGCAAAACACCAAACCccacc > 1:1751765/1‑76 (MQ=255) gCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GG‑GAATCAGCCCCAATAACAGCAAAACGCCAATCCccccca > 1:2354231/1‑76 (MQ=255) cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTT‑GG‑GAAACAGCCCCAAAAAACACAAAAAGCCCAACcccccccg > 1:2927440/1‑74 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGTCTTGCTGTC‑GG‑GAATAAGGCCCCATAACAGCAAAAAGCCAAACCCCACCAGa > 2:3676798/1‑76 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTT‑GGTAAAAAAGCCCCACAAAAAG‑AAAACACCAAAACccaccaaa > 2:1505523/1‑74 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GG‑GAATAAGCCCCAAAAAACGCAAAAAGCCAATCCccaccaca > 1:4993388/1‑74 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCCCCAGa < 2:1836117/76‑1 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGGT‑GG‑GAATTAACCCCAAAAACAACAAAAAGCCAAACCccacccaa > 2:4780126/1‑73 (MQ=255) aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGG‑AAACCGGCCCCCA‑AACAGCCAAACGCCAATCCCCCCCAGa > 2:4696903/1‑76 (MQ=255) tCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GG‑GAAACAGCCCCAATAACAGCAAAACGCCAAAACccaccacaa > 1:4136720/1‑73 (MQ=255) ccGGCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GG‑GAAACAGCCCCAATAAAAGCAAAACGCCAAACCCCCCCCGATa > 1:180629/1‑76 (MQ=255) cGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa < 1:3753322/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa < 1:2553074/76‑1 (MQ=255) ggCGTCCAGGCGCGGCTGGGCTGGCTGTC‑GG‑GAAACAGCCCCCATAAAAGCAAAAAGCCCAACCCCCCCCGATaaa > 2:3894983/1‑76 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt < 2:4691699/76‑1 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt < 2:3380906/76‑1 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt < 2:2985736/76‑1 (MQ=255) tCCAGGCGCGGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt < 2:3137974/76‑1 (MQ=255) ccAGGCGCGGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg < 1:4891741/76‑1 (MQ=255) ccAGGCGCGGCTGGGCTGGCTGTC‑GG‑GAATCAGCCCCCATAAAAACCAAACGCCAAACCCCACCCAATAAAGCaag > 2:1254213/1‑74 (MQ=255) cgcgGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGcc < 2:3081458/76‑1 (MQ=255) gcgGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 2:2411704/76‑1 (MQ=255) gcgGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt < 1:1809741/76‑1 (MQ=255) cgGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg < 2:5034390/76‑1 (MQ=255) ggCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt < 1:4827899/76‑1 (MQ=255) cTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt > 1:503763/1‑76 (MQ=255) tGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:1674639/76‑1 (MQ=255) gTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa > 2:1157845/1‑76 (MQ=255) gTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 2:3045507/76‑1 (MQ=255) gTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa < 2:2653647/76‑1 (MQ=255) tCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 2:4766946/76‑1 (MQ=255) tCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 2:4613769/76‑1 (MQ=255) cTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc > 1:850924/1‑76 (MQ=255) cTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc > 1:383064/1‑76 (MQ=255) cTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc < 2:2682336/76‑1 (MQ=255) cTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc < 1:1116629/76‑1 (MQ=255) ggCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg < 1:1503739/76‑1 (MQ=255) ggCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg < 1:2842807/76‑1 (MQ=255) cTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc > 1:425346/1‑76 (MQ=255) tGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt > 2:2247534/1‑76 (MQ=255) gTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt > 1:4009871/1‑76 (MQ=255) gTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt < 2:1369425/76‑1 (MQ=255) tC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt < 2:3167882/76‑1 (MQ=255) tC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt > 1:1943561/1‑76 (MQ=255) tC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt < 2:2977429/76‑1 (MQ=255) c‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt < 1:1247901/76‑1 (MQ=255) c‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt > 2:3613358/1‑76 (MQ=255) gg‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt > 1:3362851/1‑76 (MQ=255) gg‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt < 2:693082/76‑1 (MQ=255) g‑AAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 2:3722915/76‑1 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 2:3495550/1‑76 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 1:113896/1‑76 (MQ=255) aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc > 2:2466170/1‑76 (MQ=255) aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc > 2:4510820/1‑76 (MQ=255) aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg < 1:2381854/76‑1 (MQ=255) tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa < 1:140468/76‑1 (MQ=255) cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt < 2:2695193/76‑1 (MQ=255) cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt < 1:5121866/76‑1 (MQ=255) cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt < 2:2808510/76‑1 (MQ=255) cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt < 2:2190106/76‑1 (MQ=255) cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt > 2:1873061/1‑76 (MQ=255) cAGCCCCAATAACAGCAAAACGCCAAACCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt < 2:1379282/76‑1 (MQ=255) aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg > 1:3196823/1‑76 (MQ=255) aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg < 1:1515032/76‑1 (MQ=255) aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg > 1:3024199/1‑76 (MQ=255) aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg > 1:4885288/1‑76 (MQ=255) ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc > 1:3519609/1‑76 (MQ=255) ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc < 1:486141/76‑1 (MQ=255) ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTATTTACCGATGgc > 2:3253765/1‑76 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa > 2:3821301/1‑76 (MQ=255) cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa < 2:3128454/76‑1 (MQ=255) aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt > 2:633921/1‑76 (MQ=255) aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt < 2:5069971/76‑1 (MQ=255) aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc > 2:2095281/1‑76 (MQ=255) aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 2:4187502/1‑76 (MQ=255) aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc < 1:2111638/76‑1 (MQ=255) aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 2:2395642/1‑76 (MQ=255) aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc > 2:1097666/1‑76 (MQ=255) aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa < 2:4662984/76‑1 (MQ=255) aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa < 1:4876143/76‑1 (MQ=255) aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa > 2:1853138/1‑76 (MQ=255) gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc < 2:4683399/76‑1 (MQ=255) gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc > 2:904283/1‑76 (MQ=255) gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc < 2:2317177/76‑1 (MQ=255) aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa < 2:631894/76‑1 (MQ=255) aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg > 2:1832023/1‑76 (MQ=255) aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTATCGATGGCGCATCGCGCACCAgc > 1:3935532/1‑76 (MQ=255) aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc > 1:871668/1‑76 (MQ=255) aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc < 2:2265133/76‑1 (MQ=255) cGCCAACCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAGGGCGCATCGCGCCCCAgccg < 2:1854104/76‑1 (MQ=255) gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgc > 1:2303942/1‑76 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 2:4134982/1‑76 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 1:3768948/1‑76 (MQ=255) ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc > 1:2230657/1‑76 (MQ=255) ccAACCCCCACCAGATAAAGCATGCCGCCTGTATTAAACAGGCTTTTTACCGATAGCGCATCGCGCCCCAgccgcc < 2:732661/76‑1 (MQ=255) cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca > 2:4499862/1‑76 (MQ=255) | GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GG‑AAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA > REL606/2193706‑2193851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |