| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,488,791 | A→C | F139C (TTC→TGC) | glpR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,488,791 | 0 | A | C | 99.1% | 483.5 / ‑4.5 | 115 | F139C (TTC→TGC) | glpR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (51/63); total (52/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
AACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTG > REL606/3488719‑3488865 | aaCTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGcaaa < 2:4269070/76‑1 (MQ=255) cTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAatg > 2:3150108/1‑76 (MQ=255) tGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAatga > 1:3351193/1‑76 (MQ=255) tGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAatga > 2:4415212/1‑76 (MQ=255) tGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAatga > 1:2453508/1‑76 (MQ=255) tGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAatga > 1:4524273/1‑76 (MQ=255) tGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGGAAATCGCCACCGGCGAAAatga > 1:675094/1‑76 (MQ=255) gggAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAatgat < 2:2513633/76‑1 (MQ=255) gggAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAatgat > 2:4456354/1‑76 (MQ=255) gggAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAatgat < 1:3854911/76‑1 (MQ=255) ggAGAAAAAATCGAGCGCCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAatgatg < 2:389444/76‑1 (MQ=255) agaTAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCg < 1:2140850/76‑1 (MQ=255) aTAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGa > 1:36665/1‑76 (MQ=255) tAAAATCGAGGGTCGCTTCGCCAATAATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGaa < 1:113671/76‑1 (MQ=255) tAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGaa > 1:2211882/1‑76 (MQ=255) aaaaTGGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGaaa < 1:1660611/76‑1 (MQ=255) aaaaTCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGaaa < 1:403588/76‑1 (MQ=255) aaaTCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAAt < 1:4368623/76‑1 (MQ=255) aaaTCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAAt < 1:2713194/76‑1 (MQ=255) aaaTCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAAt < 2:1483274/76‑1 (MQ=255) aaTCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATc > 1:2995562/1‑76 (MQ=255) tCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCtt > 1:1335495/1‑76 (MQ=255) tCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCtt < 1:4933023/76‑1 (MQ=255) tCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCtt > 1:1749532/1‑76 (MQ=255) cGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTg > 2:3416478/1‑76 (MQ=255) cGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTg > 2:4494095/1‑76 (MQ=255) gAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGt > 1:2733358/1‑76 (MQ=255) gCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGttt < 2:159370/76‑1 (MQ=255) cGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGtttt > 1:3183326/1‑76 (MQ=255) cGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGtttt < 2:2340276/76‑1 (MQ=255) gTCCCTTCGCCAATGCCCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTa < 1:3083313/76‑1 (MQ=255) cGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTAcc < 1:4058040/76‑1 (MQ=255) gCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGTTTCGGAAATCTTGTCTTACCa > 1:3862607/1‑76 (MQ=255) gCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCa < 1:349629/76‑1 (MQ=255) gCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCa < 1:4342274/76‑1 (MQ=255) cTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCAt > 1:3555360/1‑76 (MQ=255) cTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCAt > 1:4804958/1‑76 (MQ=255) cTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCATGTTTTACCAt > 2:5070308/1‑76 (MQ=255) ttCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATc < 2:4454279/76‑1 (MQ=255) ttCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATc > 2:2076863/1‑76 (MQ=255) tCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCCAATGATGCGGAAATCTTGTTTTACCATCa > 2:4063110/1‑76 (MQ=255) cGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCaa < 2:4858288/76‑1 (MQ=255) gCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAAc > 1:1372244/1‑76 (MQ=255) cAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACgt > 1:1975253/1‑76 (MQ=255) cAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACgt > 2:4718215/1‑76 (MQ=255) cAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACgt > 2:4258065/1‑76 (MQ=255) aaTGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACgtg < 2:3210173/76‑1 (MQ=255) tGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGtt > 2:4064671/1‑76 (MQ=255) tGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGtt < 1:4046300/76‑1 (MQ=255) gATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTa > 1:3874821/1‑76 (MQ=255) gATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTa > 1:4993473/1‑76 (MQ=255) aTCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAg > 2:964530/1‑76 (MQ=255) tCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGc > 2:1938154/1‑76 (MQ=255) ccGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCaa < 1:4171720/76‑1 (MQ=255) cGCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAAc < 2:941915/76‑1 (MQ=255) gCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACa < 1:1492088/76‑1 (MQ=255) gCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACa < 2:4504293/76‑1 (MQ=255) gCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACa < 2:1982525/76‑1 (MQ=255) gCCATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACa < 2:4804871/76‑1 (MQ=255) ccATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACAt < 1:4811745/76‑1 (MQ=255) cATCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACAtt > 2:4277832/1‑76 (MQ=255) aTCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTg < 2:698115/76‑1 (MQ=255) aTCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTg < 2:4446342/76‑1 (MQ=255) aTCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTg < 2:3206461/76‑1 (MQ=255) aTCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTg > 2:3291615/1‑76 (MQ=255) aTCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTg > 1:4048156/1‑76 (MQ=255) tCGCGGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTga > 2:1067626/1‑76 (MQ=255) cgcgGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTgag < 2:3094567/76‑1 (MQ=255) gcgGCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTgaga > 1:1762417/1‑76 (MQ=255) ggCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGAtt < 1:2382792/76‑1 (MQ=255) ggCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGAtt < 1:3372982/76‑1 (MQ=255) gCTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGAttg > 2:3436907/1‑76 (MQ=255) cTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGAttgt > 1:3849063/1‑76 (MQ=255) cTGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGAttgt < 1:3186936/76‑1 (MQ=255) tGCGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGAttgtc < 1:4776151/76‑2 (MQ=255) cGTAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTgg < 1:980230/76‑1 (MQ=255) tAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTg < 1:2721204/76‑1 (MQ=255) tAATTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTg < 1:2484690/76‑1 (MQ=255) aaTTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGa > 2:1857201/1‑76 (MQ=255) aaTTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGa < 1:2220776/76‑1 (MQ=255) aaTTCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGa > 1:5001160/1‑76 (MQ=255) ttCGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACa < 2:3745365/76‑1 (MQ=255) cGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAAt < 2:1080094/76‑1 (MQ=255) cGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAAt > 1:3510370/1‑76 (MQ=255) cGCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAAt < 1:2646306/76‑1 (MQ=255) gCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATa < 2:1270479/76‑1 (MQ=255) gCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATa < 2:1355035/76‑1 (MQ=255) gCCACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATa < 2:2642432/76‑1 (MQ=255) ccACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATAc < 1:2641633/76‑1 (MQ=255) ccACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATAc > 2:4249909/1‑76 (MQ=255) cACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACg < 2:2292594/76‑1 (MQ=255) cACCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACg < 2:3717561/76‑1 (MQ=255) aCCGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGc > 2:536403/1‑76 (MQ=255) ccGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCa > 2:2711631/1‑76 (MQ=255) ccGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCa < 1:1821299/76‑1 (MQ=255) ccGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCa > 2:4114369/1‑76 (MQ=255) ccGGCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCa < 1:4149124/76‑1 (MQ=255) ggCGCAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCaaa < 2:1632509/76‑1 (MQ=255) gcgcAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAAt < 2:777355/76‑1 (MQ=255) gAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTg > 2:4192993/1‑76 (MQ=255) | AACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTG > REL606/3488719‑3488865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |